Journal: Chemmedchem

Article Title: Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells

doi: 10.1002/cmdc.70308

Figure Lengend Snippet: Cytotoxic effect of SQ‐B1 in SK‐MEL‐5 cells pretreated or not with NAC. MTT assay results expressed as percentage of cell viability (%), obtained from three independent experiments ( n = 3) performed in triplicate. Data were analyzed by one‐way ANOVA followed by Tukey’s test. Different letters denote significant differences among conditions ( p < 0.05).

Article Snippet: Molecular dynamics confirmed stable interactions between SQ‐B1 and GSR (RMSD plateau ~10 ns) with a total interaction energy of −201.0 kJ/mol.

Techniques: MTT Assay

Journal: Chemmedchem

Article Title: Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells

doi: 10.1002/cmdc.70308

Figure Lengend Snippet: Effect of SQ‐B1 on GSH/GSSG (reduced to oxidized) levels in SK‐MEL‐5 cells measured after 24 h treatment. Quantification of the GSH/GSSG ratio by mass spectrometry. Values were normalized to protein content and expressed as µM/mg of protein. Data were obtained from two independent experiments ( n = 2) performed in duplicate and were analyzed by one‐way ANOVA followed by Tukey’s test (* p < 0.05 vs. control; # p < 0.05 vs. SQ‐B1 4 µM).

Article Snippet: Molecular dynamics confirmed stable interactions between SQ‐B1 and GSR (RMSD plateau ~10 ns) with a total interaction energy of −201.0 kJ/mol.

Techniques: Mass Spectrometry, Control

Journal: Chemmedchem

Article Title: Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells

doi: 10.1002/cmdc.70308

Figure Lengend Snippet: Effect of SQ‐B1 on superoxide anion (O 2 • ‒ ) and nitric oxide (NO•) production in SK‐MEL‐5 cells following 24 h treatment with NAC (3 mM), SQ‐B1 (4 µM), SQ‐B1 + NAC, or Cytokines (IL‐1β 10 ng/mL, TNF‐α 100 ng/mL, and IFN‐γ 10 ng/mL), used as a positive control. (A‐C) O 2 • ‒ production measured inside the cells (A), outside the cells (B), and total O 2 • ‒ levels (C). (D‐F) NO• production measured inside the cells (D), outside the cells (E), and total NO• levels (F) under the same experimental conditions. Data are expressed as arbitrary units (a.u.) normalized to µg of protein. Results represent four independent experiments ( n = 4) performed in duplicate. Statistical analysis was performed using one‐way ANOVA followed by Tukey’s test (* p < 0.05, ** p < 0.01 vs. control).

Article Snippet: Molecular dynamics confirmed stable interactions between SQ‐B1 and GSR (RMSD plateau ~10 ns) with a total interaction energy of −201.0 kJ/mol.

Techniques: Positive Control, Control

Journal: Chemmedchem

Article Title: Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells

doi: 10.1002/cmdc.70308

Figure Lengend Snippet: Molecular docking analysis of the compound with target proteins: 3D binding poses and 2D interaction maps. (A,B). 3D structures between 6‐hydroxy‐3‐oxo‐3 H ‐xanthene‐9‐propionic acid (A) and SQ‐B1 (B) against glutathione reductase. (C,D). Amino acid interactions between the same molecules.

Article Snippet: Molecular dynamics confirmed stable interactions between SQ‐B1 and GSR (RMSD plateau ~10 ns) with a total interaction energy of −201.0 kJ/mol.

Techniques: Binding Assay

Journal: Chemmedchem

Article Title: Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells

doi: 10.1002/cmdc.70308

Figure Lengend Snippet: (A) Root mean square deviation (RMSD) of the protein backbone in complex with PDBL (blue) and SQ‐B1 (red) during 100 ns of molecular dynamics simulation. (B) Root mean square fluctuation (RMSF) of residues in the protein backbone for complexes with PDBL (blue) and SQ‐B1 (red) across the 100 ns trajectory.

Article Snippet: Molecular dynamics confirmed stable interactions between SQ‐B1 and GSR (RMSD plateau ~10 ns) with a total interaction energy of −201.0 kJ/mol.

Techniques: