maa1  (Vector Laboratories)


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    Vector Laboratories maa1
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins <t>MAA1,</t> MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Maa1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maa1/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    maa1 - by Bioz Stars, 2024-05
    94/100 stars

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    1) Product Images from "MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33"

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2019.1665971

    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Figure Legend Snippet: Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Techniques Used: Immunocytochemistry

    Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.
    Figure Legend Snippet: Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Techniques Used: Clone Assay

    biotinylated maackia amurensis lectin  (Vector Laboratories)


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  • 94

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    Vector Laboratories biotinylated maackia amurensis lectin
    Biotinylated Maackia Amurensis Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis lectin/product/Vector Laboratories
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    94/100 stars

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    sialic acids  (Vector Laboratories)


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  • 94

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    Vector Laboratories sialic acids
    Sialic Acids, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maackia amurensis  (Vector Laboratories)


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  • 94

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    Vector Laboratories biotinylated maackia amurensis
    Lectin specificity.
    Biotinylated Maackia Amurensis, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    biotinylated maackia amurensis - by Bioz Stars, 2024-05
    94/100 stars

    Images

    1) Product Images from "Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells"

    Article Title: Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells

    Journal: Journal of Extracellular Vesicles

    doi: 10.1080/20013078.2019.1648995

    Lectin specificity.
    Figure Legend Snippet: Lectin specificity.

    Techniques Used:

    biotinylated maackia amurensis ii  (Vector Laboratories)


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  • 94

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    Vector Laboratories biotinylated maackia amurensis ii
    Lectin specificity.
    Biotinylated Maackia Amurensis Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis ii/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
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    biotinylated maackia amurensis ii - by Bioz Stars, 2024-05
    94/100 stars

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    1) Product Images from "Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells"

    Article Title: Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells

    Journal: Journal of Extracellular Vesicles

    doi: 10.1080/20013078.2019.1648995

    Lectin specificity.
    Figure Legend Snippet: Lectin specificity.

    Techniques Used:

    biotinylated maackia amurensis maa  (Vector Laboratories)


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  • 94

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    Vector Laboratories biotinylated maackia amurensis maa
    Biotinylated Maackia Amurensis Maa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis maa/product/Vector Laboratories
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    biotinylated maa lectin i  (Vector Laboratories)


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    Vector Laboratories biotinylated maa lectin i
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Lectin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maa lectin i/product/Vector Laboratories
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    1) Product Images from "Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris"

    Article Title: Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0001551

    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Figure Legend Snippet: (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Techniques Used: Fluorescence, Purification, Recombinant, Activity Assay, Cell Culture, Staining

    biotinylated maackia amurensis lectin ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin ii
    Biotinylated Maackia Amurensis Lectin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maackia amurensis lectin ii mal ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin ii mal ii
    Biotinylated Maackia Amurensis Lectin Ii Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories maa1
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins <t>MAA1,</t> MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Maa1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maa1/product/Vector Laboratories
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    94
    Vector Laboratories biotinylated maackia amurensis lectin
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins <t>MAA1,</t> MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Biotinylated Maackia Amurensis Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis lectin/product/Vector Laboratories
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    Vector Laboratories sialic acids
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins <t>MAA1,</t> MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Sialic Acids, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis
    Lectin specificity.
    Biotinylated Maackia Amurensis, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis/product/Vector Laboratories
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    Vector Laboratories biotinylated maackia amurensis ii
    Lectin specificity.
    Biotinylated Maackia Amurensis Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis maa
    Lectin specificity.
    Biotinylated Maackia Amurensis Maa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis maa/product/Vector Laboratories
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    Vector Laboratories biotinylated maa lectin i
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Lectin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis lectin ii
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maackia Amurensis Lectin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Vector Laboratories biotinylated maa ii
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Vector Laboratories biotinylated maackia amurensis lectin ii mal ii
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maackia Amurensis Lectin Ii Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Journal: Emerging Microbes & Infections

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    doi: 10.1080/22221751.2019.1665971

    Figure Lengend Snippet: Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Article Snippet: Cells seeded on glass coverslips were stained with biotinylated SNA (Vector Labs, Cat# B-1305-2), MAA1 (Vector Labs, Cat# B-1315-2), MAA2 (Vector Labs, Cat# B-1265-1), DBA (Vector Labs, Cat# B-1035-5) or mouse monoclonal CT-1 antibody (courtesy of Dr Karl Klisch) and then with alkaline phosphate conjugated streptavidin (Vector Labs).

    Techniques: Immunocytochemistry

    Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Journal: Emerging Microbes & Infections

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    doi: 10.1080/22221751.2019.1665971

    Figure Lengend Snippet: Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Article Snippet: Cells seeded on glass coverslips were stained with biotinylated SNA (Vector Labs, Cat# B-1305-2), MAA1 (Vector Labs, Cat# B-1315-2), MAA2 (Vector Labs, Cat# B-1265-1), DBA (Vector Labs, Cat# B-1035-5) or mouse monoclonal CT-1 antibody (courtesy of Dr Karl Klisch) and then with alkaline phosphate conjugated streptavidin (Vector Labs).

    Techniques: Clone Assay

    Lectin specificity.

    Journal: Journal of Extracellular Vesicles

    Article Title: Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells

    doi: 10.1080/20013078.2019.1648995

    Figure Lengend Snippet: Lectin specificity.

    Article Snippet: Glycan-specific lectins were used to identify glycan groups: biotinylated Concanavalin A (ConA, specific for high mannose, terminal mannose, bi-antennary glycans, α-linked mannose, Vector Laboratories, B-1005–5), biotinylated Galanthus nivalis (GNA, specific for α1-3 mannose), biotinylated Helix pomatia agglutinin (HPA, specific for αGalNAc (Tn antigen) and type A erythrocytes, Sigma Aldrich, L6512), biotinylated Lotus Tetragonolobus (LTA, specific for α1-6 polymannose, Vector Laboratories, B-1325–2), biotinylated Maackia Amurensis I (MAL-1, specific for O -linked α-2,3 sialic acids, Vector laboratories, B-1315), biotinylated Maackia Amurensis II (MAL-II, specific for N -linked α-2,3 sialic acids, Vector laboratories, B-1265), biotinylated Sambucus nigra (SNA, Specific for N- linked α-2,6 sialic acids, Vector laboratories, B-1305) ( ).

    Techniques:

    Lectin specificity.

    Journal: Journal of Extracellular Vesicles

    Article Title: Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells

    doi: 10.1080/20013078.2019.1648995

    Figure Lengend Snippet: Lectin specificity.

    Article Snippet: Glycan-specific lectins were used to identify glycan groups: biotinylated Concanavalin A (ConA, specific for high mannose, terminal mannose, bi-antennary glycans, α-linked mannose, Vector Laboratories, B-1005–5), biotinylated Galanthus nivalis (GNA, specific for α1-3 mannose), biotinylated Helix pomatia agglutinin (HPA, specific for αGalNAc (Tn antigen) and type A erythrocytes, Sigma Aldrich, L6512), biotinylated Lotus Tetragonolobus (LTA, specific for α1-6 polymannose, Vector Laboratories, B-1325–2), biotinylated Maackia Amurensis I (MAL-1, specific for O -linked α-2,3 sialic acids, Vector laboratories, B-1315), biotinylated Maackia Amurensis II (MAL-II, specific for N -linked α-2,3 sialic acids, Vector laboratories, B-1265), biotinylated Sambucus nigra (SNA, Specific for N- linked α-2,6 sialic acids, Vector laboratories, B-1305) ( ).

    Techniques:

    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Journal: PLoS ONE

    Article Title: Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris

    doi: 10.1371/journal.pone.0001551

    Figure Lengend Snippet: (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Article Snippet: After washing, the cells were incubated at 37°C for 15 min with 10 µg/ml of biotinylated MAA lectin I (Vector Laboratories, Burlingame, CA), which was prepared with 1% (w/v) bovine serum albumin (BSA) in PBS.

    Techniques: Fluorescence, Purification, Recombinant, Activity Assay, Cell Culture, Staining