biotinylated dolichos biflorus agglutinin (dba)  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin (dba)
    Biotinylated Dolichos Biflorus Agglutinin (Dba), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated dolichos biflorus agglutinin (dba)  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin (dba)
    Biotinylated Dolichos Biflorus Agglutinin (Dba), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dba  (Vector Laboratories)


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    Vector Laboratories dba
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with <t>lectins</t> <t>MAA1,</t> MAA2,SNA, and <t>DBA</t> and monoclonal antibody CT-1. Scale bar = 100 μm.
    Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dba/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dba - by Bioz Stars, 2024-05
    94/100 stars

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    1) Product Images from "MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33"

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2019.1665971

    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.
    Figure Legend Snippet: Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Techniques Used: Immunocytochemistry

    Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.
    Figure Legend Snippet: Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Techniques Used: Clone Assay

    biotinylated dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin
    Biotinylated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated dolichos biflorus agglutinin dba  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin dba
    Biotinylated Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated dolichos biflorus agglutinin lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin lectin
    Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos <t>biflorus</t> agglutinin (DBA) <t>lectin.</t> eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.
    Biotinylated Dolichos Biflorus Agglutinin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated dolichos biflorus agglutinin lectin/product/Vector Laboratories
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    Images

    1) Product Images from "High fat feeding promotes obesity and renal inflammation and protects against post cardiopulmonary bypass acute kidney injury in swine"

    Article Title: High fat feeding promotes obesity and renal inflammation and protects against post cardiopulmonary bypass acute kidney injury in swine

    Journal: Critical Care

    doi: 10.1186/cc13092

    Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos biflorus agglutinin (DBA) lectin. eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.
    Figure Legend Snippet: Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos biflorus agglutinin (DBA) lectin. eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.

    Techniques Used: Immunofluorescence, Immunocytochemistry, Staining, Filtration, Preserving

    biotinylated dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin
    Biotinylated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dba  (Vector Laboratories)


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    Vector Laboratories dba
    Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated dolichos biflorus agglutinin dba lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin dba lectin
    A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. <t>DBA</t> <t>lectin</t> staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).
    Biotinylated Dolichos Biflorus Agglutinin Dba Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Notch Signaling Regulates Bile Duct Morphogenesis in Mice"

    Article Title: Notch Signaling Regulates Bile Duct Morphogenesis in Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0001851

    A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. DBA lectin staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).
    Figure Legend Snippet: A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. DBA lectin staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).

    Techniques Used: Staining, Immunostaining

    dba biotinylated  (Vector Laboratories)


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    Vector Laboratories dba biotinylated
    Primary antibodies
    Dba Biotinylated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Axon fasciculation in the developing olfactory nerve"

    Article Title: Axon fasciculation in the developing olfactory nerve

    Journal: Neural Development

    doi: 10.1186/1749-8104-5-20

    Primary antibodies
    Figure Legend Snippet: Primary antibodies

    Techniques Used: Plasmid Preparation

    biotinylated dolichos biflorus agglutinin  (Vector Laboratories)


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    Vector Laboratories biotinylated dolichos biflorus agglutinin
    Biotinylated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated dolichos biflorus agglutinin (dba)
    Biotinylated Dolichos Biflorus Agglutinin (Dba), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories dba
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with <t>lectins</t> <t>MAA1,</t> MAA2,SNA, and <t>DBA</t> and monoclonal antibody CT-1. Scale bar = 100 μm.
    Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated dolichos biflorus agglutinin
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with <t>lectins</t> <t>MAA1,</t> MAA2,SNA, and <t>DBA</t> and monoclonal antibody CT-1. Scale bar = 100 μm.
    Biotinylated Dolichos Biflorus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated dolichos biflorus agglutinin/product/Vector Laboratories
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    Vector Laboratories biotinylated dolichos biflorus agglutinin dba
    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with <t>lectins</t> <t>MAA1,</t> MAA2,SNA, and <t>DBA</t> and monoclonal antibody CT-1. Scale bar = 100 μm.
    Biotinylated Dolichos Biflorus Agglutinin Dba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated dolichos biflorus agglutinin dba/product/Vector Laboratories
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    Vector Laboratories biotinylated dolichos biflorus agglutinin lectin
    Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos <t>biflorus</t> agglutinin (DBA) <t>lectin.</t> eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.
    Biotinylated Dolichos Biflorus Agglutinin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated dolichos biflorus agglutinin dba lectin
    A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. <t>DBA</t> <t>lectin</t> staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).
    Biotinylated Dolichos Biflorus Agglutinin Dba Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories dba biotinylated
    Primary antibodies
    Dba Biotinylated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Journal: Emerging Microbes & Infections

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    doi: 10.1080/22221751.2019.1665971

    Figure Lengend Snippet: Immunocytochemistry of MDCK, MDCK-SiaT1 and MDCK-B4GalNT2 cells with lectins MAA1, MAA2,SNA, and DBA and monoclonal antibody CT-1. Scale bar = 100 μm.

    Article Snippet: Cells seeded on glass coverslips were stained with biotinylated SNA (Vector Labs, Cat# B-1305-2), MAA1 (Vector Labs, Cat# B-1315-2), MAA2 (Vector Labs, Cat# B-1265-1), DBA (Vector Labs, Cat# B-1035-5) or mouse monoclonal CT-1 antibody (courtesy of Dr Karl Klisch) and then with alkaline phosphate conjugated streptavidin (Vector Labs).

    Techniques: Immunocytochemistry

    Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Journal: Emerging Microbes & Infections

    Article Title: MDCK-B4GalNT2 cells disclose a α2,3-sialic acid requirement for the 2009 pandemic H1N1 A/California/04/2009 and NA aid entry of A/WSN/33

    doi: 10.1080/22221751.2019.1665971

    Figure Lengend Snippet: Enzyme-linked lectin assay of cell lysates from MDCK, MDCK-SiaT1 (SiaT1), MDCK-YFP (YFP) and 3 MDCK-B4GalNT2 clones bound by (A)MAA1, (B) MAA2, (C) SNA_and (D) DBA. Signals are normalized to wild-type MDCK cells. Mean ± 1sd, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, one way-ANOVA.

    Article Snippet: Cells seeded on glass coverslips were stained with biotinylated SNA (Vector Labs, Cat# B-1305-2), MAA1 (Vector Labs, Cat# B-1315-2), MAA2 (Vector Labs, Cat# B-1265-1), DBA (Vector Labs, Cat# B-1035-5) or mouse monoclonal CT-1 antibody (courtesy of Dr Karl Klisch) and then with alkaline phosphate conjugated streptavidin (Vector Labs).

    Techniques: Clone Assay

    Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos biflorus agglutinin (DBA) lectin. eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.

    Journal: Critical Care

    Article Title: High fat feeding promotes obesity and renal inflammation and protects against post cardiopulmonary bypass acute kidney injury in swine

    doi: 10.1186/cc13092

    Figure Lengend Snippet: Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos biflorus agglutinin (DBA) lectin. eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. * P <0.05 versus sham, † P <0.05 versus Sham + HF, ‡ P <0.05 versus CPB.

    Article Snippet: Vascular endothelium was identified by percent staining of immunocytochemistry for biotinylated dolichos biflorus agglutinin lectin (Vector Laboratories).

    Techniques: Immunofluorescence, Immunocytochemistry, Staining, Filtration, Preserving

    A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. DBA lectin staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).

    Journal: PLoS ONE

    Article Title: Notch Signaling Regulates Bile Duct Morphogenesis in Mice

    doi: 10.1371/journal.pone.0001851

    Figure Lengend Snippet: A,B. Hematoxylin and eosin-stained sections at P7 of control littermate (CT) and Notch2-cko mice using the Notch2 del2 allele. Bile ducts (arrow) are observed in the periportal region of the control littermate (A), but not the Notch2-cko mouse (B). C–F. DBA lectin staining. C,D. Control littermate and Notch2-cko mice using the Notch2 del2 allele at P7. E,F. Control littermate and Notch2-cko mice using the Notch2 del3 allele at P3. DBA-positive cells form patent bile ducts (arrows) adjacent to the portal veins in control mice (C,E). In Notch2-cko mice using either the Notch2 del2 (D) or Notch2 del3 (F) allele, small numbers of DBA-positive cells (arrowheads) are present adjacent to the portal veins, but these cells have not formed patent ducts. G,H. Cytokeratin immunostaining of control littermate and Notch2-cko mice ( Notch2 del2 allele) at P7. The ductal plate of the control liver (G) has remodeled into epithelial bile ducts (arrows), while the Notch2-cko liver (H) exhibits only ductal plate remnants (arrowheads).

    Article Snippet: The antibodies and lectins used in these studies were rabbit polyclonal anti-human cytokeratins (Dako, Cat. A0575); rabbit polyclonal anti-HNF1β (Santa Cruz, Cat. sc-22840); rabbit polyclonal anti-HNF6 (Santa Cruz, Cat. sc-13050); and biotinylated Dolichos biflorus agglutinin (DBA) lectin (Vector Laboratories, Cat. B-1035).

    Techniques: Staining, Immunostaining

    Primary antibodies

    Journal: Neural Development

    Article Title: Axon fasciculation in the developing olfactory nerve

    doi: 10.1186/1749-8104-5-20

    Figure Lengend Snippet: Primary antibodies

    Article Snippet: DBA (Biotinylated) , Vector Laboratories , NA , 1:50.

    Techniques: Plasmid Preparation