wheat germ agglutinin succ wga  (Vector Laboratories)


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    Name:
    Biotinylated succinylated Wheat Germ Agglutinin WGA
    Description:
    Wheat germ agglutinin WGA This derivative has been reported to have properties distinct from the native lectin Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues unlike the native form but retains its specificity toward N acetylglucosamine Biotinylated succinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide
    Catalog Number:
    B-1025S
    Price:
    None
    Size:
    5 mg
    Category:
    Proteins
    Buy from Supplier


    Structured Review

    Vector Laboratories wheat germ agglutinin succ wga
    Biotinylated succinylated Wheat Germ Agglutinin WGA
    Wheat germ agglutinin WGA This derivative has been reported to have properties distinct from the native lectin Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues unlike the native form but retains its specificity toward N acetylglucosamine Biotinylated succinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide
    https://www.bioz.com/result/wheat germ agglutinin succ wga/product/Vector Laboratories
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin succ wga - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "Human secretory immunoglobulin A may contribute to biofilm formation in the gut"

    Article Title: Human secretory immunoglobulin A may contribute to biofilm formation in the gut

    Journal: Immunology

    doi: 10.1046/j.1365-2567.2003.01700.x

    Exposure of ‘neoepitopes’ on cultured human epithelial cells following incubation with human enteric bacteria. The binding of succinylated wheat germ agglutinin (succ-WGA) and sIgA were evaluated using ELA as described in the Materials and Methods section. The binding of succ-WGA was used to evaluate the exposure of saccharides normally buried on the cell surface, as described in the text. Twelve wells were used for each condition, and the standard errors are shown. The experiment shown is representative of three experiments performed.
    Figure Legend Snippet: Exposure of ‘neoepitopes’ on cultured human epithelial cells following incubation with human enteric bacteria. The binding of succinylated wheat germ agglutinin (succ-WGA) and sIgA were evaluated using ELA as described in the Materials and Methods section. The binding of succ-WGA was used to evaluate the exposure of saccharides normally buried on the cell surface, as described in the text. Twelve wells were used for each condition, and the standard errors are shown. The experiment shown is representative of three experiments performed.

    Techniques Used: Cell Culture, Incubation, Binding Assay, Whole Genome Amplification

    2) Product Images from "Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin"

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin

    Journal: Stem Cells International

    doi: 10.1155/2019/4341286

    Evaluation of AC-GlcNAc coatings. (a) Structure of AC-GlcNAc. (b) Evaluation of AC-GlcNAc coating on polystyrene dishes using the interaction between biotinylated sWGA and AC-GlcNAc. The amount of biotinylated sWGA on dishes was measured by HRP-neutravidin and TMB solution (absorbance at 450 nm). (c) Western blots demonstrating expression of cell-surface vimentin in human immortalized MSCs. (d) UE7T-13 adherence to AC-GlcNAc-coated dishes at 37 (left) and 4°C (right). ∗ P
    Figure Legend Snippet: Evaluation of AC-GlcNAc coatings. (a) Structure of AC-GlcNAc. (b) Evaluation of AC-GlcNAc coating on polystyrene dishes using the interaction between biotinylated sWGA and AC-GlcNAc. The amount of biotinylated sWGA on dishes was measured by HRP-neutravidin and TMB solution (absorbance at 450 nm). (c) Western blots demonstrating expression of cell-surface vimentin in human immortalized MSCs. (d) UE7T-13 adherence to AC-GlcNAc-coated dishes at 37 (left) and 4°C (right). ∗ P

    Techniques Used: Western Blot, Expressing

    3) Product Images from "Molecular Dissection of the secA2 Locus of Group B Streptococcus Reveals that Glycosylation of the Srr1 LPXTG Protein Is Required for Full Virulence ▿ Locus of Group B Streptococcus Reveals that Glycosylation of the Srr1 LPXTG Protein Is Required for Full Virulence ▿ †"

    Article Title: Molecular Dissection of the secA2 Locus of Group B Streptococcus Reveals that Glycosylation of the Srr1 LPXTG Protein Is Required for Full Virulence ▿ Locus of Group B Streptococcus Reveals that Glycosylation of the Srr1 LPXTG Protein Is Required for Full Virulence ▿ †

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01673-08

    Lectin-binding patterns of S. agalactiae NEM316 and isogenic mutant derivatives. GBS strains were immobilized within the wells of 96-well plates, and the binding of seven biotinylated lectins was quantitated with streptavidin-conjugated HRP. sWGA, succinylated
    Figure Legend Snippet: Lectin-binding patterns of S. agalactiae NEM316 and isogenic mutant derivatives. GBS strains were immobilized within the wells of 96-well plates, and the binding of seven biotinylated lectins was quantitated with streptavidin-conjugated HRP. sWGA, succinylated

    Techniques Used: Binding Assay, Mutagenesis

    4) Product Images from "Modification of Streptococcus mutans Cnm by PgfS Contributes to Adhesion, Endothelial Cell Invasion, and Virulence"

    Article Title: Modification of Streptococcus mutans Cnm by PgfS Contributes to Adhesion, Endothelial Cell Invasion, and Virulence

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01783-14

    WGA and sWGA lectin analysis of Cnm. Detection and identification of carbohydrates attached to Cnm in S. mutans strains using biotinylated lectins is shown. Whole-cell lysates were separated by SDS-PAGE, transferred onto PVDF membranes, and probed with 20 μg ml −1 WGA (A) or 20 μg ml −1 sWGA (C). (B) Purified Cnm was treated with sialidase for 20 h and probed with WGA. (D) WGA detection of purified Cnm was blocked using increasing concentrations of N -acetylglucosamine.
    Figure Legend Snippet: WGA and sWGA lectin analysis of Cnm. Detection and identification of carbohydrates attached to Cnm in S. mutans strains using biotinylated lectins is shown. Whole-cell lysates were separated by SDS-PAGE, transferred onto PVDF membranes, and probed with 20 μg ml −1 WGA (A) or 20 μg ml −1 sWGA (C). (B) Purified Cnm was treated with sialidase for 20 h and probed with WGA. (D) WGA detection of purified Cnm was blocked using increasing concentrations of N -acetylglucosamine.

    Techniques Used: Whole Genome Amplification, SDS Page, Purification

    Related Articles

    Concentration Assay:

    Article Title: GtfA and GtfB Are Both Required for Protein O-Glycosylation in Lactobacillus plantarum
    Article Snippet: .. The membranes were then incubated with biotinylated sWGA (Vector Laboratories, Burlingame, CA; final concentration of 14.3 μg/ml), Dolichos biflorus lectin (Sigma-Aldrich, Zwijndrecht, the Netherlands; final concentration of 14.3 μg/ml), or Lens culinaris lectin (EY Labs Inc., San Mateo, CA; final concentration of 5 μg/ml) in the blocking solution, followed by incubation with 0.1 nl/ml (1:10,000 dilution) of streptavidin poly-horseradish peroxidase (poly-HRP; ImmunoTools GmbH, Friesoythe, Germany). ..

    Incubation:

    Article Title: Modification of Streptococcus mutans Cnm by PgfS Contributes to Adhesion, Endothelial Cell Invasion, and Virulence
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 1 h at room temperature, the membranes were incubated with 20 μg ml−1 of biotinylated wheat germ agglutinin (WGA), succinylated wheat germ agglutinin (sWGA), concanavalin A (ConA), or peanut agglutinin (PNA) (Vector Laboratories, Burlingame, CA) in PBS containing 0.5% BSA for 1 h at room temperature. .. Membranes were washed three times with PBS containing 0.1% Tween 20, followed by incubation with HRP-conjugated streptavidin (Cell Signaling Technology, Danvers, MA).

    Article Title: GtfA and GtfB Are Both Required for Protein O-Glycosylation in Lactobacillus plantarum
    Article Snippet: .. The membranes were then incubated with biotinylated sWGA (Vector Laboratories, Burlingame, CA; final concentration of 14.3 μg/ml), Dolichos biflorus lectin (Sigma-Aldrich, Zwijndrecht, the Netherlands; final concentration of 14.3 μg/ml), or Lens culinaris lectin (EY Labs Inc., San Mateo, CA; final concentration of 5 μg/ml) in the blocking solution, followed by incubation with 0.1 nl/ml (1:10,000 dilution) of streptavidin poly-horseradish peroxidase (poly-HRP; ImmunoTools GmbH, Friesoythe, Germany). ..

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
    Article Snippet: .. After blocking, wells were incubated with 100 μ L of 0.25 μ g/mL biotinylated-succinylated wheat germ agglutinin (sWGA) (Vector Laboratories, Inc., Burlingame, CA, USA) in PBS for 2 h at 25°C. .. After incubation, the wells were incubated with 100 μ L of 0.05 μ g/mL horseradish peroxidase- (HRP-) conjugated NeutrAvidin (Thermo Fisher Scientific Inc., Waltham, MA, USA) for 2 h at 25°C, and 100 μ L of substrate TMB solution (SeraCare Life Sciences, Inc., Milford, MA, USA) was added to each well.

    Whole Genome Amplification:

    Article Title: Modification of Streptococcus mutans Cnm by PgfS Contributes to Adhesion, Endothelial Cell Invasion, and Virulence
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 1 h at room temperature, the membranes were incubated with 20 μg ml−1 of biotinylated wheat germ agglutinin (WGA), succinylated wheat germ agglutinin (sWGA), concanavalin A (ConA), or peanut agglutinin (PNA) (Vector Laboratories, Burlingame, CA) in PBS containing 0.5% BSA for 1 h at room temperature. .. Membranes were washed three times with PBS containing 0.1% Tween 20, followed by incubation with HRP-conjugated streptavidin (Cell Signaling Technology, Danvers, MA).

    Article Title: The Cytoplasmic Tail Dileucine Motif LL572 Determines the Glycosylation Pattern of Membrane-type 1 Matrix Metalloproteinase *
    Article Snippet: .. For lectin precipitation, 900 μl of cell lysate were preincubated with 25 μg of biotinylated wheat germ agglutinin (WGA) or biotinylated, succinylated wheat germ agglutinin (sWGA) (Vector Laboratories, Burlingame, CA) for 4 h at 4 °C before 60 μl of a washed 50% slurry of streptavidin conjugated beads (Pierce Biotechnology, Rockford, IL) were added. .. After overnight incubation at 4 °C on a rotating platform, beads were washed five times in 1% Triton X-100 lysis buffer.

    Avidin-Biotin Assay:

    Article Title: A Putative O-Linked β-N-Acetylglucosamine Transferase Is Essential for Hormogonium Development and Motility in the Filamentous Cyanobacterium Nostoc punctiforme
    Article Snippet: .. Detection of O-GlcNAcylated proteins using the anti- O -GlcNAc monoclonal antibody CTD110.6 (Enzo Life Sciences, Inc.) in conjunction with goat anti-mouse IgG–horseradish-conjugated peroxidase (HRP) (sc2005; Santa Cruz Biotechnology), or biotinylated succinylated wheat germ agglutinin (sWGA) (Vector Laboratories) in conjunction with the avidin-HRP conjugate Vectastain ABC reagent, was performed as previously described ( , ). ..

    Blocking Assay:

    Article Title: Modification of Streptococcus mutans Cnm by PgfS Contributes to Adhesion, Endothelial Cell Invasion, and Virulence
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 1 h at room temperature, the membranes were incubated with 20 μg ml−1 of biotinylated wheat germ agglutinin (WGA), succinylated wheat germ agglutinin (sWGA), concanavalin A (ConA), or peanut agglutinin (PNA) (Vector Laboratories, Burlingame, CA) in PBS containing 0.5% BSA for 1 h at room temperature. .. Membranes were washed three times with PBS containing 0.1% Tween 20, followed by incubation with HRP-conjugated streptavidin (Cell Signaling Technology, Danvers, MA).

    Article Title: GtfA and GtfB Are Both Required for Protein O-Glycosylation in Lactobacillus plantarum
    Article Snippet: .. The membranes were then incubated with biotinylated sWGA (Vector Laboratories, Burlingame, CA; final concentration of 14.3 μg/ml), Dolichos biflorus lectin (Sigma-Aldrich, Zwijndrecht, the Netherlands; final concentration of 14.3 μg/ml), or Lens culinaris lectin (EY Labs Inc., San Mateo, CA; final concentration of 5 μg/ml) in the blocking solution, followed by incubation with 0.1 nl/ml (1:10,000 dilution) of streptavidin poly-horseradish peroxidase (poly-HRP; ImmunoTools GmbH, Friesoythe, Germany). ..

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin
    Article Snippet: .. After blocking, wells were incubated with 100 μ L of 0.25 μ g/mL biotinylated-succinylated wheat germ agglutinin (sWGA) (Vector Laboratories, Inc., Burlingame, CA, USA) in PBS for 2 h at 25°C. .. After incubation, the wells were incubated with 100 μ L of 0.05 μ g/mL horseradish peroxidase- (HRP-) conjugated NeutrAvidin (Thermo Fisher Scientific Inc., Waltham, MA, USA) for 2 h at 25°C, and 100 μ L of substrate TMB solution (SeraCare Life Sciences, Inc., Milford, MA, USA) was added to each well.

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  • 96
    Vector Laboratories biotinylated wga
    Optimization of SPA binding to HBE cultures The localization of KS (A), MUC1 (B), and <t>WGA</t> (C) were studied in primary HBE cells as indicated under “Experimental Procedures.” Anti-KS antibody and WGA bound to the apical plasma membrane ( arrow ) and cilia ( arrowhead ), while MUC1 antibody recognized mostly the apical plasma membrane ( arrow ). Bar = 10 μ m. D , binding of SPA-HRP to apical HBE cell surface as quantitated with the colorimetric reaction of HRP and o -phenylenediamine under varied antibody/lectin concentrations. Live primary HBE cultures were incubated with anti-KS antibody ( blue diamond ), anti-MUC1 antibody ( orange triangle ), or <t>biotinylated</t> WGA ( green circle ) at indicated concentration. WGA binding quantitation required incubation with 2 μ g/ml anti-biotin antibody. All samples were subsequently incubated with 1 μ g/ml SPA-HRP ( solid symbols ) or buffer ( open symbols ). Values are mean ± S.E. of three Transwells/subject established from two different subjects.
    Biotinylated Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated wga/product/Vector Laboratories
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    biotinylated wga - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    93
    Vector Laboratories wheat germ agglutinin succ wga
    Exposure of ‘neoepitopes’ on cultured human epithelial cells following incubation with human enteric bacteria. The binding of succinylated wheat germ agglutinin <t>(succ-WGA)</t> and sIgA were evaluated using ELA as described in the Materials and Methods section. The binding of succ-WGA was used to evaluate the exposure of saccharides normally buried on the cell surface, as described in the text. Twelve wells were used for each condition, and the standard errors are shown. The experiment shown is representative of three experiments performed.
    Wheat Germ Agglutinin Succ Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin succ wga/product/Vector Laboratories
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin succ wga - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Optimization of SPA binding to HBE cultures The localization of KS (A), MUC1 (B), and WGA (C) were studied in primary HBE cells as indicated under “Experimental Procedures.” Anti-KS antibody and WGA bound to the apical plasma membrane ( arrow ) and cilia ( arrowhead ), while MUC1 antibody recognized mostly the apical plasma membrane ( arrow ). Bar = 10 μ m. D , binding of SPA-HRP to apical HBE cell surface as quantitated with the colorimetric reaction of HRP and o -phenylenediamine under varied antibody/lectin concentrations. Live primary HBE cultures were incubated with anti-KS antibody ( blue diamond ), anti-MUC1 antibody ( orange triangle ), or biotinylated WGA ( green circle ) at indicated concentration. WGA binding quantitation required incubation with 2 μ g/ml anti-biotin antibody. All samples were subsequently incubated with 1 μ g/ml SPA-HRP ( solid symbols ) or buffer ( open symbols ). Values are mean ± S.E. of three Transwells/subject established from two different subjects.

    Journal: The Journal of biological chemistry

    Article Title: Physiological Regulation of ATP Release at the Apical Surface of Human Airway Epithelia *

    doi: 10.1074/jbc.M603019200

    Figure Lengend Snippet: Optimization of SPA binding to HBE cultures The localization of KS (A), MUC1 (B), and WGA (C) were studied in primary HBE cells as indicated under “Experimental Procedures.” Anti-KS antibody and WGA bound to the apical plasma membrane ( arrow ) and cilia ( arrowhead ), while MUC1 antibody recognized mostly the apical plasma membrane ( arrow ). Bar = 10 μ m. D , binding of SPA-HRP to apical HBE cell surface as quantitated with the colorimetric reaction of HRP and o -phenylenediamine under varied antibody/lectin concentrations. Live primary HBE cultures were incubated with anti-KS antibody ( blue diamond ), anti-MUC1 antibody ( orange triangle ), or biotinylated WGA ( green circle ) at indicated concentration. WGA binding quantitation required incubation with 2 μ g/ml anti-biotin antibody. All samples were subsequently incubated with 1 μ g/ml SPA-HRP ( solid symbols ) or buffer ( open symbols ). Values are mean ± S.E. of three Transwells/subject established from two different subjects.

    Article Snippet: The solution was replaced with 50 μ l of PBS/BSA containing various concentrations of an anti-MUC1 antibody (mouse IgG2b , ab8323, Abcam, Cambridge, MA), an anti-KS antibody (mouse IgG2b , Chemicon, Temecula, CA), or biotinylated WGA (Vector Laboratories, Burlingame, CA).

    Techniques: Binding Assay, Whole Genome Amplification, Incubation, Concentration Assay, Quantitation Assay

    Exposure of ‘neoepitopes’ on cultured human epithelial cells following incubation with human enteric bacteria. The binding of succinylated wheat germ agglutinin (succ-WGA) and sIgA were evaluated using ELA as described in the Materials and Methods section. The binding of succ-WGA was used to evaluate the exposure of saccharides normally buried on the cell surface, as described in the text. Twelve wells were used for each condition, and the standard errors are shown. The experiment shown is representative of three experiments performed.

    Journal: Immunology

    Article Title: Human secretory immunoglobulin A may contribute to biofilm formation in the gut

    doi: 10.1046/j.1365-2567.2003.01700.x

    Figure Lengend Snippet: Exposure of ‘neoepitopes’ on cultured human epithelial cells following incubation with human enteric bacteria. The binding of succinylated wheat germ agglutinin (succ-WGA) and sIgA were evaluated using ELA as described in the Materials and Methods section. The binding of succ-WGA was used to evaluate the exposure of saccharides normally buried on the cell surface, as described in the text. Twelve wells were used for each condition, and the standard errors are shown. The experiment shown is representative of three experiments performed.

    Article Snippet: Biotinylated, succinylated wheat germ agglutinin (succ-WGA) was obtained from Vector Laboratories, Inc. (Burlingame, CA).

    Techniques: Cell Culture, Incubation, Binding Assay, Whole Genome Amplification

    Evaluation of AC-GlcNAc coatings. (a) Structure of AC-GlcNAc. (b) Evaluation of AC-GlcNAc coating on polystyrene dishes using the interaction between biotinylated sWGA and AC-GlcNAc. The amount of biotinylated sWGA on dishes was measured by HRP-neutravidin and TMB solution (absorbance at 450 nm). (c) Western blots demonstrating expression of cell-surface vimentin in human immortalized MSCs. (d) UE7T-13 adherence to AC-GlcNAc-coated dishes at 37 (left) and 4°C (right). ∗ P

    Journal: Stem Cells International

    Article Title: Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin

    doi: 10.1155/2019/4341286

    Figure Lengend Snippet: Evaluation of AC-GlcNAc coatings. (a) Structure of AC-GlcNAc. (b) Evaluation of AC-GlcNAc coating on polystyrene dishes using the interaction between biotinylated sWGA and AC-GlcNAc. The amount of biotinylated sWGA on dishes was measured by HRP-neutravidin and TMB solution (absorbance at 450 nm). (c) Western blots demonstrating expression of cell-surface vimentin in human immortalized MSCs. (d) UE7T-13 adherence to AC-GlcNAc-coated dishes at 37 (left) and 4°C (right). ∗ P

    Article Snippet: After blocking, wells were incubated with 100 μ L of 0.25 μ g/mL biotinylated-succinylated wheat germ agglutinin (sWGA) (Vector Laboratories, Inc., Burlingame, CA, USA) in PBS for 2 h at 25°C.

    Techniques: Western Blot, Expressing