pik93  (Echelon Biosciences)


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    Echelon Biosciences pik93
    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with <t>PIK93</t> or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Pik93, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pik93/product/Echelon Biosciences
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    pik93 - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    2) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    3) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    4) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    5) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    6) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    7) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    8) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    9) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    10) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    11) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    12) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    13) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    14) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    15) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    16) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    17) Product Images from "Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1"

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700243

    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Figure Legend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Techniques Used: Inhibition, Concentration Assay, Binding Assay

    18) Product Images from "PKC-ε Regulates Vesicle Delivery and Focal Exocytosis for Efficient IgG-mediated Phagocytosis"

    Article Title: PKC-ε Regulates Vesicle Delivery and Focal Exocytosis for Efficient IgG-mediated Phagocytosis

    Journal: bioRxiv

    doi: 10.1101/2021.05.07.443102

    PKC-ε delivery is reversibly inhibited by nocodazole or PIK93 BMDM expressing PKC-ε -GFP were treated with DMSO (Solvent control), PIK93 (100nM), or nocodazole (10 μM) for 30 min then spread on IgG surface for 30 min and fixed. For washouts, the inhibitor-containing media was replaced with HBSS + + for 5 min prior to fixation. Fire LUT was applied for optimal visualization of puncta, inserts represent GFP expression. Samples were imaged by TIRFM and the number of puncta was quantitated by FIJI software. Data are presented as number of puncta/cell area to account for differences in cell spreading. Data are presented as mean ± SEM of 3 independent experiments (total of 39-45 cells per condition), each data point represents a cell. Statistical significance was determined by one-way ANOVA with Bonferroni post-test. ****p
    Figure Legend Snippet: PKC-ε delivery is reversibly inhibited by nocodazole or PIK93 BMDM expressing PKC-ε -GFP were treated with DMSO (Solvent control), PIK93 (100nM), or nocodazole (10 μM) for 30 min then spread on IgG surface for 30 min and fixed. For washouts, the inhibitor-containing media was replaced with HBSS + + for 5 min prior to fixation. Fire LUT was applied for optimal visualization of puncta, inserts represent GFP expression. Samples were imaged by TIRFM and the number of puncta was quantitated by FIJI software. Data are presented as number of puncta/cell area to account for differences in cell spreading. Data are presented as mean ± SEM of 3 independent experiments (total of 39-45 cells per condition), each data point represents a cell. Statistical significance was determined by one-way ANOVA with Bonferroni post-test. ****p

    Techniques Used: Expressing, Software

    PIK93 Treatment Does Not Alter Golgi Structure 3D projections of RAW cells treated with DMSO or 100nM PIK93 and stained for (A) cis-medial Golgi marker, GM130, or (B) trans-Golgi marker (Golgin-245). Images were collected with spinning-disc confocal microscopy. Imaris generated 3D renderings visualize overall Golgi structure (right panels). Scale bar = 10 μm. n = 3 (33-36 cells per condition/treatment).
    Figure Legend Snippet: PIK93 Treatment Does Not Alter Golgi Structure 3D projections of RAW cells treated with DMSO or 100nM PIK93 and stained for (A) cis-medial Golgi marker, GM130, or (B) trans-Golgi marker (Golgin-245). Images were collected with spinning-disc confocal microscopy. Imaris generated 3D renderings visualize overall Golgi structure (right panels). Scale bar = 10 μm. n = 3 (33-36 cells per condition/treatment).

    Techniques Used: Staining, Marker, Confocal Microscopy, Generated

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    Echelon Biosciences pik93
    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with <t>PIK93</t> or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p
    Pik93, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pik93/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
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    Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Golgi-associated PKC-ε is delivered to phagocytic cups: Role of PI4P

    doi: 10.4049/jimmunol.1700243

    Figure Lengend Snippet: Inhibition of PI4K blocks membrane fusion, PKC-ε concentration at cups, and slows target uptake (A) Wild type BMDM, treated with PIK93 or Wt, and PKC-ε -/- ) and tracked from attachment to phagosome closure; 0 defines the time of target binding. Bars = 2 μm. From the movies, PKC-ε concentration at cups (C) and the rate of internalization (D) were determined. Results are reported as mean ± SEM (n = 40-49 events compiled from 3 independent experiments). Significance was tested by ANOVA with Bonferroni's correction. *** p

    Article Snippet: Using whole cell patch clamping, we determined that cells treated with Wt or PIK93 have significantly lower capacitance than solvent treated controls ( ).

    Techniques: Inhibition, Concentration Assay, Binding Assay