b per  (Thermo Fisher)


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    Thermo Fisher b per
    B Per, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b per/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b per - by Bioz Stars, 2022-11
    97/100 stars

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    Thermo Fisher b per reagent
    B Per Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b per reagent/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b per reagent - by Bioz Stars, 2022-11
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    97
    Thermo Fisher bacterial protein extraction reagent
    Comparison of OTS efficiency upon alteration of the number and spacing pattern of in-frame stop codons. Ribosomal incorporation of m - o NB-Dopa (1 mM) into ELP-sfGFP constructs containing a varying number of in-frame amber stop codons. Constructs are shown as pairs of identical ELP fragment length (e.g. ELP(5x amber) and ELP(8x amber)). Normal = one amber stop codon <t>per</t> three VPGXG repeats, equidistant placement. Alternating = alternating amber stop codons, see Supplementary Material for sequence details. Intact cell fluorescence of E. coli <t>B-95.ΔA,</t> endpoint measurements after 18 h of incubation. Data (incl. Standard deviation) represent the mean of three biological replicates.
    Bacterial Protein Extraction Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial protein extraction reagent/product/Thermo Fisher
    Average 97 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    bacterial protein extraction reagent - by Bioz Stars, 2022-11
    97/100 stars
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    Comparison of OTS efficiency upon alteration of the number and spacing pattern of in-frame stop codons. Ribosomal incorporation of m - o NB-Dopa (1 mM) into ELP-sfGFP constructs containing a varying number of in-frame amber stop codons. Constructs are shown as pairs of identical ELP fragment length (e.g. ELP(5x amber) and ELP(8x amber)). Normal = one amber stop codon per three VPGXG repeats, equidistant placement. Alternating = alternating amber stop codons, see Supplementary Material for sequence details. Intact cell fluorescence of E. coli B-95.ΔA, endpoint measurements after 18 h of incubation. Data (incl. Standard deviation) represent the mean of three biological replicates.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Engineered bacterial host for genetic encoding of physiologically stable protein nitration

    doi: 10.3389/fmolb.2022.992748

    Figure Lengend Snippet: Comparison of OTS efficiency upon alteration of the number and spacing pattern of in-frame stop codons. Ribosomal incorporation of m - o NB-Dopa (1 mM) into ELP-sfGFP constructs containing a varying number of in-frame amber stop codons. Constructs are shown as pairs of identical ELP fragment length (e.g. ELP(5x amber) and ELP(8x amber)). Normal = one amber stop codon per three VPGXG repeats, equidistant placement. Alternating = alternating amber stop codons, see Supplementary Material for sequence details. Intact cell fluorescence of E. coli B-95.ΔA, endpoint measurements after 18 h of incubation. Data (incl. Standard deviation) represent the mean of three biological replicates.

    Article Snippet: Harvested cell pellets were resuspended (50 mM sodium phosphate, 300 mM NaCl, 20 mM imidazole, pH 8.0) and lysed with B-PER Bacterial Protein Extraction Reagent (ThermoFisher Scientific, Waltham, MA, United States) according to protocol, with the addition of phenylmethanesulfonyl fluoride (PMSF, 1 mM final concentration), DNAse and RNAse.

    Techniques: Construct, Sequencing, Fluorescence, Incubation, Standard Deviation