b multivorans atcc 17616  (ATCC)


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    Name:
    Burkholderia multivorans 249 S 4 An
    Description:

    Catalog Number:
    17616
    Price:
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    Applications:
    Genomic analysisOpportunistic pathogen researchRespiratory research
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    Structured Review

    ATCC b multivorans atcc 17616
    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. <t>multivorans</t> <t>ATCC</t> 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

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    Images

    1) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    2) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    3) Product Images from "“Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates"

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00181-19

    (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.
    Figure Legend Snippet: (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Techniques Used: Activity Assay, Purification

    4) Product Images from "Comparative Genome Sequence Analysis Reveals the Extent of Diversity and Conservation for Glycan-Associated Proteins in Burkholderia spp."

    Article Title: Comparative Genome Sequence Analysis Reveals the Extent of Diversity and Conservation for Glycan-Associated Proteins in Burkholderia spp.

    Journal: Comparative and Functional Genomics

    doi: 10.1155/2012/752867

    Multiple sequence alignment showed glycosylated amino acid in (a) BPSL1902, (b) BPSS1434, and (c) BPSS2053 with its respective orthologs. The amino acids in red represent O-glycosylation; blue color represents N-glycosylation and secondary structures shown are B. pseudomallei K96243 secondary structures. Note. BPSL1902, BPSS1434, and BPSS2053 are B. pseudomallei K96243 protein, B_mallei23344 is B. mallei ATCC 23344, B_mv17616 is B. multivorans ATCC 17616, B_ambifariaMC40-6 is B. ambifaria MC40-6, B_vietG4 is B. vietnamiensis G4, B_cenoJ2315 is B. cenocepacia J2315, B_thailE264 is B. thailandensis E264, and B_xenoLB400 is B. xenovorans LB400.
    Figure Legend Snippet: Multiple sequence alignment showed glycosylated amino acid in (a) BPSL1902, (b) BPSS1434, and (c) BPSS2053 with its respective orthologs. The amino acids in red represent O-glycosylation; blue color represents N-glycosylation and secondary structures shown are B. pseudomallei K96243 secondary structures. Note. BPSL1902, BPSS1434, and BPSS2053 are B. pseudomallei K96243 protein, B_mallei23344 is B. mallei ATCC 23344, B_mv17616 is B. multivorans ATCC 17616, B_ambifariaMC40-6 is B. ambifaria MC40-6, B_vietG4 is B. vietnamiensis G4, B_cenoJ2315 is B. cenocepacia J2315, B_thailE264 is B. thailandensis E264, and B_xenoLB400 is B. xenovorans LB400.

    Techniques Used: Sequencing

    Glycosylated serine and threonine tandem repeats in (a) BPSS1434, (b) BPSS1439 and (c) BPSL1705 with its respective orthologs in Burkholderia spp. (if any). The amino acids in red represent O-glycosylation; blue color represents N-glycosylation. Note. BPSL1434, BPSL1705, and BPSS1439 are B. pseudomallei K96243 proteins, B_mv17616 is B. multivorans ATCC 17616, B_ambifariaMC40-6 is B. ambifaria MC40-6, B_vietG4 is B. vietnamiensis G4, B_thailE264 is B. thailandensis E264, and B_xenoLB400 is B. xenovorans LB400.
    Figure Legend Snippet: Glycosylated serine and threonine tandem repeats in (a) BPSS1434, (b) BPSS1439 and (c) BPSL1705 with its respective orthologs in Burkholderia spp. (if any). The amino acids in red represent O-glycosylation; blue color represents N-glycosylation. Note. BPSL1434, BPSL1705, and BPSS1439 are B. pseudomallei K96243 proteins, B_mv17616 is B. multivorans ATCC 17616, B_ambifariaMC40-6 is B. ambifaria MC40-6, B_vietG4 is B. vietnamiensis G4, B_thailE264 is B. thailandensis E264, and B_xenoLB400 is B. xenovorans LB400.

    Techniques Used:

    5) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    6) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    7) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    8) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    9) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    10) Product Images from "Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans"

    Article Title: Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00602-11

    Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.
    Figure Legend Snippet: Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.

    Techniques Used: Permeability

    Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans
    Figure Legend Snippet: Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans

    Techniques Used: Binding Assay, Mutagenesis, Fluorescence

    Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published
    Figure Legend Snippet: Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published

    Techniques Used: Mutagenesis

    11) Product Images from "Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans"

    Article Title: Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00602-11

    Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.
    Figure Legend Snippet: Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.

    Techniques Used: Permeability

    Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans
    Figure Legend Snippet: Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans

    Techniques Used: Binding Assay, Mutagenesis, Fluorescence

    Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published
    Figure Legend Snippet: Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published

    Techniques Used: Mutagenesis

    12) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    13) Product Images from "Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro"

    Article Title: Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0082522

    Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.
    Figure Legend Snippet: Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.

    Techniques Used: Incubation, Variant Assay

    14) Product Images from "Genomic analysis and relatedness of P2-like phages of the Burkholderia cepacia complex"

    Article Title: Genomic analysis and relatedness of P2-like phages of the Burkholderia cepacia complex

    Journal: BMC Genomics

    doi: 10.1186/1471-2164-11-599

    Isolation of the KS14 plasmid prophage . DNA was isolated using a QIAprep Spin Miniprep plasmid isolation kit (Qiagen) and digested with EcoRI (Invitrogen). Lane 1: 1 Kb Plus DNA ladder (Invitrogen), lane 2: B. cenocepacia C6433, lane 3: B. multivorans ATCC 17616, lane 4: B. cenocepacia CEP511 lane 5: B. cenocepacia K56-2, lane 6: blank, lane 7: 1 Kb Plus DNA ladder, lane 8: KS14-resistant C6433 isolate I, lane 9: KS14-resistant C6433 isolate II, lane 10: KS14-resistant C6433 isolate III, lane 11: KS14-resistant C6433 isolate IV, lane 12: KS14-resistant C6433 isolate V. The size of the markers (in kbp) is shown on the left. A KS14 EcoRI DNA digest and the size of the bands predicted for this digest ( > 1 kbp in size) are shown on the far right.
    Figure Legend Snippet: Isolation of the KS14 plasmid prophage . DNA was isolated using a QIAprep Spin Miniprep plasmid isolation kit (Qiagen) and digested with EcoRI (Invitrogen). Lane 1: 1 Kb Plus DNA ladder (Invitrogen), lane 2: B. cenocepacia C6433, lane 3: B. multivorans ATCC 17616, lane 4: B. cenocepacia CEP511 lane 5: B. cenocepacia K56-2, lane 6: blank, lane 7: 1 Kb Plus DNA ladder, lane 8: KS14-resistant C6433 isolate I, lane 9: KS14-resistant C6433 isolate II, lane 10: KS14-resistant C6433 isolate III, lane 11: KS14-resistant C6433 isolate IV, lane 12: KS14-resistant C6433 isolate V. The size of the markers (in kbp) is shown on the left. A KS14 EcoRI DNA digest and the size of the bands predicted for this digest ( > 1 kbp in size) are shown on the far right.

    Techniques Used: Isolation, Plasmid Preparation

    15) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    16) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    17) Product Images from "The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616"

    Article Title: The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00479-17

    Organizations of hemP and hmu genes in B. multivorans ATCC 17616 and comparison with those in other species. (A) Comparison of the ATCC 17616 genes with those in other bacterial species. Genes are color-coded and are annotated as follows: hemP , regulator of hmu operon; hmuR , TonB-dependent outer membrane heme receptor; hmuS , cytoplasmic heme-binding protein/heme oxygenase; hmuT , periplasmic heme-binding protein; hmuU , permease subunit of inner membrane heme transporter; hmuV , ATP-binding cassette subunit of inner membrane heme transporter. Chr. 1 and Chr. 2, chromosomes 1 and 2, respectively. The red lines indicate the binding sites of Fur or its functional homologue. Abbreviations of strain names are as follows: Bmul, Burkholderia multivorans ATCC 17616; Bcen, Burkholderia cenocepacia J2315; Bmal, Burkholderia mallei ATCC 23344; Bpse, Burkholderia pseudomallei 1710b; Yent, Yersinia enterocolitica 0:8; Bjap, Bradyrhizobium japonicum USDA110; Smel, Sinorhizobium meliloti 1021; Ecol, Escherichia coli O157:H7. The hemP homologues from B. japonicum and S. meliloti are designated hmuP . Numbers under the maps show the percent identity of amino acid sequences, compared with that encoded by ATCC 17616. (B) Sequence upstream of hemP in the ATCC 17616 genome. The putative Fur box is shown in red.
    Figure Legend Snippet: Organizations of hemP and hmu genes in B. multivorans ATCC 17616 and comparison with those in other species. (A) Comparison of the ATCC 17616 genes with those in other bacterial species. Genes are color-coded and are annotated as follows: hemP , regulator of hmu operon; hmuR , TonB-dependent outer membrane heme receptor; hmuS , cytoplasmic heme-binding protein/heme oxygenase; hmuT , periplasmic heme-binding protein; hmuU , permease subunit of inner membrane heme transporter; hmuV , ATP-binding cassette subunit of inner membrane heme transporter. Chr. 1 and Chr. 2, chromosomes 1 and 2, respectively. The red lines indicate the binding sites of Fur or its functional homologue. Abbreviations of strain names are as follows: Bmul, Burkholderia multivorans ATCC 17616; Bcen, Burkholderia cenocepacia J2315; Bmal, Burkholderia mallei ATCC 23344; Bpse, Burkholderia pseudomallei 1710b; Yent, Yersinia enterocolitica 0:8; Bjap, Bradyrhizobium japonicum USDA110; Smel, Sinorhizobium meliloti 1021; Ecol, Escherichia coli O157:H7. The hemP homologues from B. japonicum and S. meliloti are designated hmuP . Numbers under the maps show the percent identity of amino acid sequences, compared with that encoded by ATCC 17616. (B) Sequence upstream of hemP in the ATCC 17616 genome. The putative Fur box is shown in red.

    Techniques Used: Binding Assay, Functional Assay, Sequencing

    18) Product Images from "Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans"

    Article Title: Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00602-11

    Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.
    Figure Legend Snippet: Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.

    Techniques Used: Permeability

    Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans
    Figure Legend Snippet: Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans

    Techniques Used: Binding Assay, Mutagenesis, Fluorescence

    Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published
    Figure Legend Snippet: Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published

    Techniques Used: Mutagenesis

    19) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    20) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    21) Product Images from "“Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates"

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00181-19

    (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.
    Figure Legend Snippet: (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Techniques Used: Activity Assay, Purification

    22) Product Images from "“Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates"

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00181-19

    (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.
    Figure Legend Snippet: (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Techniques Used: Activity Assay, Purification

    23) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    24) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    25) Product Images from "The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616"

    Article Title: The Small Protein HemP Is a Transcriptional Activator for the Hemin Uptake Operon in Burkholderia multivorans ATCC 17616

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00479-17

    Organizations of hemP and hmu genes in B. multivorans ATCC 17616 and comparison with those in other species. (A) Comparison of the ATCC 17616 genes with those in other bacterial species. Genes are color-coded and are annotated as follows: hemP , regulator of hmu operon; hmuR , TonB-dependent outer membrane heme receptor; hmuS , cytoplasmic heme-binding protein/heme oxygenase; hmuT , periplasmic heme-binding protein; hmuU , permease subunit of inner membrane heme transporter; hmuV , ATP-binding cassette subunit of inner membrane heme transporter. Chr. 1 and Chr. 2, chromosomes 1 and 2, respectively. The red lines indicate the binding sites of Fur or its functional homologue. Abbreviations of strain names are as follows: Bmul, Burkholderia multivorans ATCC 17616; Bcen, Burkholderia cenocepacia J2315; Bmal, Burkholderia mallei ATCC 23344; Bpse, Burkholderia pseudomallei 1710b; Yent, Yersinia enterocolitica 0:8; Bjap, Bradyrhizobium japonicum USDA110; Smel, Sinorhizobium meliloti 1021; Ecol, Escherichia coli O157:H7. The hemP homologues from B. japonicum and S. meliloti are designated hmuP . Numbers under the maps show the percent identity of amino acid sequences, compared with that encoded by ATCC 17616. (B) Sequence upstream of hemP in the ATCC 17616 genome. The putative Fur box is shown in red.
    Figure Legend Snippet: Organizations of hemP and hmu genes in B. multivorans ATCC 17616 and comparison with those in other species. (A) Comparison of the ATCC 17616 genes with those in other bacterial species. Genes are color-coded and are annotated as follows: hemP , regulator of hmu operon; hmuR , TonB-dependent outer membrane heme receptor; hmuS , cytoplasmic heme-binding protein/heme oxygenase; hmuT , periplasmic heme-binding protein; hmuU , permease subunit of inner membrane heme transporter; hmuV , ATP-binding cassette subunit of inner membrane heme transporter. Chr. 1 and Chr. 2, chromosomes 1 and 2, respectively. The red lines indicate the binding sites of Fur or its functional homologue. Abbreviations of strain names are as follows: Bmul, Burkholderia multivorans ATCC 17616; Bcen, Burkholderia cenocepacia J2315; Bmal, Burkholderia mallei ATCC 23344; Bpse, Burkholderia pseudomallei 1710b; Yent, Yersinia enterocolitica 0:8; Bjap, Bradyrhizobium japonicum USDA110; Smel, Sinorhizobium meliloti 1021; Ecol, Escherichia coli O157:H7. The hemP homologues from B. japonicum and S. meliloti are designated hmuP . Numbers under the maps show the percent identity of amino acid sequences, compared with that encoded by ATCC 17616. (B) Sequence upstream of hemP in the ATCC 17616 genome. The putative Fur box is shown in red.

    Techniques Used: Binding Assay, Functional Assay, Sequencing

    26) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    27) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    28) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    29) Product Images from "Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro"

    Article Title: Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0082522

    Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.
    Figure Legend Snippet: Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.

    Techniques Used: Incubation, Variant Assay

    30) Product Images from "Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans"

    Article Title: Identification of Hopanoid Biosynthesis Genes Involved in Polymyxin Resistance in Burkholderia multivorans

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00602-11

    Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.
    Figure Legend Snippet: Effect of increasing concentrations of polymyxin B on the outer membrane permeability of B. multivorans ATCC 17616 compared to mutants 26D7 and RMI19. Polymyxin B was titrated into suspensions of whole bacteria at an OD 600 of 0.5 and 10 μM NPN.

    Techniques Used: Permeability

    Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans
    Figure Legend Snippet: Binding of DPX to whole parent and mutant cells. Bacterial cells were treated with increasing concentrations of DPX, and fluorescence was determined by emission at 485 nm upon excitation at 340 nm. ■, B. multivorans ATCC 17616; □, B. multivorans

    Techniques Used: Binding Assay, Mutagenesis, Fluorescence

    Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published
    Figure Legend Snippet: Map of the B. multivorans ATCC 17616 locus affected by Tn 5-751S in mutant 26D7. The region from base pairs 2344808 to 2350836 of the B. multivorans ) is shown in the published

    Techniques Used: Mutagenesis

    31) Product Images from "Characterization of the AmpC β-Lactamase from Burkholderia multivorans"

    Article Title: Characterization of the AmpC β-Lactamase from Burkholderia multivorans

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01140-18

    Immunoblotting reveals that protein expression of AmpC1 and PenA is inducible in B . multivorans ATCC 17616 after growth in subinhibitory concentrations of imipenem for different amounts of time. The anti-RecA antibody was used as a loading control.
    Figure Legend Snippet: Immunoblotting reveals that protein expression of AmpC1 and PenA is inducible in B . multivorans ATCC 17616 after growth in subinhibitory concentrations of imipenem for different amounts of time. The anti-RecA antibody was used as a loading control.

    Techniques Used: Expressing

    32) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    33) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    34) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    35) Product Images from "Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation"

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01343-17

    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
    Figure Legend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Techniques Used: Mutagenesis, Produced

    36) Product Images from "“Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates"

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00181-19

    (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.
    Figure Legend Snippet: (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Techniques Used: Activity Assay, Purification

    37) Product Images from "Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro"

    Article Title: Stress Conditions Triggering Mucoid Morphotype Variation in Burkholderia Species and Effect on Virulence in Galleria mellonella and Biofilm Formation In Vitro

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0082522

    Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.
    Figure Legend Snippet: Morphotype variation among clinical and environmental isolates of Burkholderia . (a) The frequency of the nonmucoid morphotype was determined under prolonged stationary phase for 21 days (black bars) and in the presence of 2.5 times the MIC of ciprofloxacin of each mucoid isolate (white bars) after 7 days of static incubation at 37°C for Bc c isolates or 30°C for non- Bc c isolates. The data are based on mean values from the results of three independent cell cultivations. Error bars show SD. Clinical isolates are depicted in bold. (b) PFGE separation of the SpeI restriction fragments of the genomic DNA from the mucoid parental isolate (P) and the nonmucoid variant (V) of: B. cepacia IST408 (1); B. multivorans ATCC 17616 (2) and D2095 (3); B. stabilis LMG18888 (4); B. dolosa CEP0743 (5); B. ambifaria CEP0958 (6); B. anthina FC0974 (7); B. phymatum STM815 (8); and B. xenovorans LB400 (9). Arrowheads indicate differences in banding patterns.

    Techniques Used: Incubation, Variant Assay

    38) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    39) Product Images from "Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient"

    Article Title: Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a Cystic Fibrosis Patient

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2017.01027

    Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.
    Figure Legend Snippet: Genetic organization of gene clusters for core-lipid A and OAg biosynthesis in B. cenocepacia IST439 (A) and the reference strains K56-2 and J2315 (B) . The flanking genes ureG and apaH are indicated in black and the four genes represented in gray encode proteins putatively involved in lipid A-core biosynthesis. The genes in dark-red correspond to genes present in IST439 without a counterpart in the reference strain J2315 (Supplementary Figure S3 ), but with a degree of homology to B. multivorans ATCC 17616 (see also Supplementary Figure S4B ). The red-vertical arrows above the genes represent the two non-synonymous point mutations in genes bmul_2510 and wbiI of the 10 sequential isolates (comparative genomic analysis to be described elsewhere). A GC content plot is also represented for IST439 [drawn using Artemis ( Carver et al., 2008 )] above the display line of the sequence, where the genes annotated as B. multivorans are highlighted in a black rectangle. wλ , 3-deoxy- D -manno-octulosonic acid transferase; kdoO , Kdo dioxygenase; waaC , heptosyltransferase I; manB , phosphomannomutase; wzx , OAg exporter; wbxA , glycosyltransferase; wbxB , glycosyltransferase; galE , UDP-glucose epimerase; wecA , UDP- N -acetylglucosamine 1-P transferase; wbiI , nucleotide sugar epimerase-dehydratase; wbiH , UDP- N -acetylglucosamine 1-P transferase; wbiG , nucleotide sugar epimerase-dehydratase; wbiF , glycosyltransferase; bmul_2508 , conserved hypothetical protein; Bmul_2509 , group 1 glycosyl transferase; Bmul_2510 , conserved hypothetical protein; Bmul_2514 , type 11 methyltransferase; wzt , ABC transporter ATP-binding protein; wzm , ABC transporter membrane permease; rmlDCAB , dTDP-rhamnose biosynthesis; wbxC , acetyltransferase; wbxD , glycosyltransferase; wbxE , glycosyltransferase; vioA , nucleotide sugar aminotransferase.

    Techniques Used: Sequencing, Binding Assay

    Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).
    Figure Legend Snippet: Genetic organization of the OAg biosynthetic gene clusters of B. multivorans strains ATCC 17616 (B) and IST419 (A) , including genes for lipid A-core biosynthesis and OAg biosynthesis within the apaH and ureG flanking genes (in black). Conserved genes among Burkholderia species involved in lipid A-core biosynthesis are indicated in gray. GC content plots are represented for both clusters [drawn using Artemis ( Carver et al., 2008 )], where the B. multivorans ATCC 17616 with homology in the corresponding cluster of B. cenocepacia IST439 are highlighted in a black rectangle. A silver nitrate-stained SDS-PAGE gel (C) shows the banding pattern of LPS samples extracted from B. cenocepacia IST439 ( Bc IST439), B. multivorans IST419 ( Bm IST419), and B. multivorans ATCC 17616 ( Bm ATCC17616).

    Techniques Used: Staining, SDS Page

    40) Product Images from "Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿"

    Article Title: Distribution of Cepacian Biosynthesis Genes among Environmental and Clinical Burkholderia Strains and Role of Cepacian Exopolysaccharide in Resistance to Stress Conditions ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.01828-09

    Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed
    Figure Legend Snippet: Protective role of EPS against desiccation and iron ion stress. Cells from overnight grown cultures of B. xenovorans LB400 (▪), B. multivorans ATCC 17616 (▴), and B. cepacia IST408 (•) were harvested by centrifugation and exposed

    Techniques Used: Centrifugation

    EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8
    Figure Legend Snippet: EPS production by Burkholderia strains. Cells from different Burkholderia species (a) and from B. cepacia IST408 (•), B. cepacia IST408 bceR ::pIS58-2 (▪), B. multivorans ATCC 17616 (▴), and B. multivorans ATCC 17616 bceS ::pSF71-8

    Techniques Used:

    Related Articles

    Mutagenesis:

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation
    Article Snippet: .. Plasmids pMM137-2, pLM016-2, and pARG015-1 were mobilized into B. multivorans ATCC 17616 and the Δ ldhR mutant by triparental conjugation. ..

    Conjugation Assay:

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation
    Article Snippet: .. Plasmids pMM137-2, pLM016-2, and pARG015-1 were mobilized into B. multivorans ATCC 17616 and the Δ ldhR mutant by triparental conjugation. ..

    Expressing:

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates
    Article Snippet: .. Moreover, piperacillin, tazobactam, ceftazidime, and avibactam, as well as combinations thereof, did not induce expression of bla penA1 and bla ampC1 in the B. multivorans ATCC 17616 background. .. When ceftazidime-avibactam is combined with piperacillin-tazobactam, the susceptibility of Bcc and B. gladioli to ceftazidime and piperacillin is restored in vitro .

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    ATCC b multivorans atcc 17616
    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. <t>multivorans</t> <t>ATCC</t> 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P
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    LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Journal: Applied and Environmental Microbiology

    Article Title: Regulator LdhR and d-Lactate Dehydrogenase LdhA of Burkholderia multivorans Play Roles in Carbon Overflow and in Planktonic Cellular Aggregate Formation

    doi: 10.1128/AEM.01343-17

    Figure Lengend Snippet: LdhR decreases exopolysaccharide production. (A) Production of EPS by the WT B. multivorans ATCC 17616 (WT) and the Δ ldhR mutant in the presence of different sugars as the main carbon source for 3 days at 37°C. EPS production is expressed as ethanol precipitate (dry weight) (g/liter). A significantly greater amount of EPS was produced by the Δ ldhR mutant. *, P

    Article Snippet: Our data on the consumption of sugars, such as d -fructose and d -mannose, and the sugar alcohol d -mannitol by B. multivorans ATCC 17616 showed their dissimilation through the phosphorylative pathway.

    Techniques: Mutagenesis, Produced

    (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Journal: Journal of Clinical Microbiology

    Article Title: “Switching Partners”: Piperacillin-Avibactam Is a Highly Potent Combination against Multidrug-Resistant Burkholderia cepacia Complex and Burkholderia gladioli Cystic Fibrosis Isolates

    doi: 10.1128/JCM.00181-19

    Figure Lengend Snippet: (A and B) B. multivorans ATCC 17616 (Bm) was grown in LB to log phase, selected β-lactam and β-lactam/β-lactamase inhibitor combinations were added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared and run on an analytic isoelectric focusing gel. A nitrocefin overlay was used to develop the gel and visualize β-lactamase activity. Purified PenA1 and AmpC1 were used as controls. Abbreviations: CAZ, ceftazidime; AVI, avibactam; PIP, piperacillin; TAZO, tazobactam. (C) Samples: pure PenA, 200 ng (lane 1); pure AmpC, 200 ng (lane 2); ATCC 17616, no induction (lane 3); ATCC 17616, with imipenem (lane 4); ATCC 17616, with piperacillin (lane 5); ATCC 17616, with piperacillin-tazobactam (lane 6); ATCC 17616, with ceftazidime (lane 7); ATCC 17616, with ceftazidime-avibactam (lane 8); ATCC 17616, with ceftazidime-piperacillin-tazobactam (lane 9); ATCC 17616, with piperacillin-tazobactam-avibactam (lane 10); ATCC 17616, with piperacillin-ceftazidime-avibactam (lane 11); ATCC 17616, with piperacillin-tazobactam-ceftazidime-avibactam (lane 12); ATCC 17616, with piperacillin-avibactam (lane 13). Cells were grown in LB to log phase, antibiotic(s) was added at sub-MIC levels, and cells were grown for an additional hour. Crude extracts were prepared, and immunoblotting was conducted with α-PenA and α-AmpC polyclonal antibodies.

    Article Snippet: Moreover, piperacillin, tazobactam, ceftazidime, and avibactam, as well as combinations thereof, did not induce expression of bla penA1 and bla ampC1 in the B. multivorans ATCC 17616 background.

    Techniques: Activity Assay, Purification