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    Structured Review

    Millipore b megaterium
    Construction and expression of B. <t>megaterium</t> tyrosinase in E. coli . ( a ) Map of the expression vector pETDuet-BmTYR. ( b ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-BmTYR. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 24 h induction (lane 3) were separated using SDS-PAGE. Arrows indicate the expressed tyrosinase. M represents the molecular weight markers. ( c ) Tyrosinase activity assay using l -DOPA as substrate. Either 20 μg (─■─), 100 μg (─▲─), or 400 μg (─●─) of the lyophilized recombinant cells was mixed with 2.5 mM of l -DOPA in 1 mL phosphate-buffered saline (PBS, pH 6.8) and the resulting dopachrome was monitored at 475 nm with a spectrophotometer. One unit of tyrosinase activity is the amount of cells that produces one μmole of dopachrome (absorption efficiency ε = 3600 cm −1 M −1 ) per minute from the reaction.
    B Megaterium, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b megaterium/product/Millipore
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b megaterium - by Bioz Stars, 2020-03
    81/100 stars

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    1) Product Images from "Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli"

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli

    Journal: Molecules

    doi: 10.3390/molecules21121723

    Construction and expression of B. megaterium tyrosinase in E. coli . ( a ) Map of the expression vector pETDuet-BmTYR. ( b ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-BmTYR. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 24 h induction (lane 3) were separated using SDS-PAGE. Arrows indicate the expressed tyrosinase. M represents the molecular weight markers. ( c ) Tyrosinase activity assay using l -DOPA as substrate. Either 20 μg (─■─), 100 μg (─▲─), or 400 μg (─●─) of the lyophilized recombinant cells was mixed with 2.5 mM of l -DOPA in 1 mL phosphate-buffered saline (PBS, pH 6.8) and the resulting dopachrome was monitored at 475 nm with a spectrophotometer. One unit of tyrosinase activity is the amount of cells that produces one μmole of dopachrome (absorption efficiency ε = 3600 cm −1 M −1 ) per minute from the reaction.
    Figure Legend Snippet: Construction and expression of B. megaterium tyrosinase in E. coli . ( a ) Map of the expression vector pETDuet-BmTYR. ( b ) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of recombinant E. coli harboring pETDuet-BmTYR. Crude proteins from whole cell lysis with 0 h induction (lane 1), 4 h induction (lane 2), and 24 h induction (lane 3) were separated using SDS-PAGE. Arrows indicate the expressed tyrosinase. M represents the molecular weight markers. ( c ) Tyrosinase activity assay using l -DOPA as substrate. Either 20 μg (─■─), 100 μg (─▲─), or 400 μg (─●─) of the lyophilized recombinant cells was mixed with 2.5 mM of l -DOPA in 1 mL phosphate-buffered saline (PBS, pH 6.8) and the resulting dopachrome was monitored at 475 nm with a spectrophotometer. One unit of tyrosinase activity is the amount of cells that produces one μmole of dopachrome (absorption efficiency ε = 3600 cm −1 M −1 ) per minute from the reaction.

    Techniques Used: Expressing, Plasmid Preparation, Polyacrylamide Gel Electrophoresis, SDS Page, Recombinant, Lysis, Molecular Weight, Activity Assay, Spectrophotometry

    Diagram of the biotransformation of the soy isoflavone glycosides daidzin and genistin by the recombinant E. coli expressing B. megaterium tyrosinase.
    Figure Legend Snippet: Diagram of the biotransformation of the soy isoflavone glycosides daidzin and genistin by the recombinant E. coli expressing B. megaterium tyrosinase.

    Techniques Used: Recombinant, Expressing

    Related Articles

    Centrifugation:

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Purified MBP (0.5 μg) in 50 μl buffer D containing 1 mM benzamidine and 0.01% 1-phenyl-2-thiourea (PTU) was mixed with the pellet from 200 μl cell suspension at 4 C for 1 h. After centrifugation at 4,000g for 15 min, the supernatant was treated with 5× SDS sample buffer and 1/5 of it analyzed as the unbound fraction. .. Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

    Amplification:

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: .. Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. The recombinant E. coli harboring the expression vector was confirmed to produce considerable tyrosinase protein (36 kDa) after isopropyl-β-d -thiogalactopyranoside (IPTG) induction ( b).

    Mutagenesis:

    Article Title: Amino Acid Substitutions in Transmembrane Domains 9 and 10 of GerVB That Affect the Germination Properties of Bacillus megaterium Spores ▿
    Article Snippet: .. Escherichia coli strains used for site-directed mutagenesis (XL1-Blue [Stratagene]) or preparation of plasmids for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. Protoplasts were prepared by inoculating 25 ml of RHAF medium ( ) with 200 μl of an overnight suspension of B. megaterium cells and incubated (37°C at 225 rpm) until an optical density at 660 nm (OD660 ) of ∼0.6 was attained.

    Cell Culture:

    Article Title: Amino Acid Substitutions in Transmembrane Domains 9 and 10 of GerVB That Affect the Germination Properties of Bacillus megaterium Spores ▿
    Article Snippet: .. Escherichia coli strains used for site-directed mutagenesis (XL1-Blue [Stratagene]) or preparation of plasmids for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. Protoplasts were prepared by inoculating 25 ml of RHAF medium ( ) with 200 μl of an overnight suspension of B. megaterium cells and incubated (37°C at 225 rpm) until an optical density at 660 nm (OD660 ) of ∼0.6 was attained.

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Beauveria bassiana was cultured on a YPG plate (0.2% yeast extract, 1% peptone, 2% glucose, 1.7% agar) at 25°C for 3 days. .. Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

    Article Title: Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor ▿
    Article Snippet: .. Escherichia coli strains used for SDM (XL1-Blue and XL10-Gold [Stratagene]) or preparation of plasmid for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. B . megaterium spores were prepared in 200-ml aliquots of supplemented nutrient broth ( ) in 2-liter flasks (at 30°C and 200 rpm) supplemented with 1 μg/ml erythromycin to provide selective pressure for maintenance of the plasmid-borne receptor operon.

    Purification:

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Purified MBP (0.5 μg) in 50 μl buffer D containing 1 mM benzamidine and 0.01% 1-phenyl-2-thiourea (PTU) was mixed with the pellet from 200 μl cell suspension at 4 C for 1 h. After centrifugation at 4,000g for 15 min, the supernatant was treated with 5× SDS sample buffer and 1/5 of it analyzed as the unbound fraction. .. Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

    Variant Assay:

    Article Title: Amino Acid Substitutions in Transmembrane Domains 9 and 10 of GerVB That Affect the Germination Properties of Bacillus megaterium Spores ▿
    Article Snippet: The Bacillus megaterium strains employed in this study (Table ) are all derivatives of strain PV361 , a plasmidless variant of the wild-type QM B1551 strain that lacks the GerU operon and GerVB structural gene required to initiate germination in response to single trigger compounds. .. Escherichia coli strains used for site-directed mutagenesis (XL1-Blue [Stratagene]) or preparation of plasmids for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin.

    Article Title: Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor ▿
    Article Snippet: B . megaterium strains employed in this study (Table ) are essentially all derivatives of strain PV361, a plasmidless variant of the wild-type QM B1551 strain that lacks the GerU operon and GerVB structural gene required for efficient spore germination. .. Escherichia coli strains used for SDM (XL1-Blue and XL10-Gold [Stratagene]) or preparation of plasmid for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin.

    Incubation:

    Article Title: Production of Interleukin-12 by Murine Macrophages in Response to Bacterial Peptidoglycan
    Article Snippet: .. A sample of PG from B. megaterium (1 mg/ml) was treated for 3 min in a boiling water bath and incubated overnight at 37°C, in sterile conditions, with 40 μg of egg white lysozyme (Sigma Chemical Co., L’Isle d’Abeau Chesnes, France) per ml in 0.15 M phosphate buffer (pH 6.2). .. The mouse macrophage cell line J774 was cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, 2 mM glutamine, 50 μg of gentamicin per ml, and 100 U of penicillin per ml.

    Selection:

    Article Title: Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor ▿
    Article Snippet: Preparation of B. megaterium protoplasts and polyethylene glycol-mediated transformation of plasmid DNA was performed as described previously , with direct selection for transformants on RHAF agar plates containing 1 μg/ml erythromycin and 25 μg/ml lincomycin. .. Escherichia coli strains used for SDM (XL1-Blue and XL10-Gold [Stratagene]) or preparation of plasmid for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin.

    Activity Assay:

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. In addition, the tyrosinase activity of the recombinant cells was determined using l -3,4-dihydroxyphenylalanine (l -DOPA) as a substrate.

    Expressing:

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: .. Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. The recombinant E. coli harboring the expression vector was confirmed to produce considerable tyrosinase protein (36 kDa) after isopropyl-β-d -thiogalactopyranoside (IPTG) induction ( b).

    Polymerase Chain Reaction:

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: .. Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. The recombinant E. coli harboring the expression vector was confirmed to produce considerable tyrosinase protein (36 kDa) after isopropyl-β-d -thiogalactopyranoside (IPTG) induction ( b).

    Recombinant:

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: .. Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. The recombinant E. coli harboring the expression vector was confirmed to produce considerable tyrosinase protein (36 kDa) after isopropyl-β-d -thiogalactopyranoside (IPTG) induction ( b).

    Transformation Assay:

    Article Title: Amino Acid Substitutions in Transmembrane Domains 9 and 10 of GerVB That Affect the Germination Properties of Bacillus megaterium Spores ▿
    Article Snippet: .. Escherichia coli strains used for site-directed mutagenesis (XL1-Blue [Stratagene]) or preparation of plasmids for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. Protoplasts were prepared by inoculating 25 ml of RHAF medium ( ) with 200 μl of an overnight suspension of B. megaterium cells and incubated (37°C at 225 rpm) until an optical density at 660 nm (OD660 ) of ∼0.6 was attained.

    Article Title: Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor ▿
    Article Snippet: .. Escherichia coli strains used for SDM (XL1-Blue and XL10-Gold [Stratagene]) or preparation of plasmid for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. B . megaterium spores were prepared in 200-ml aliquots of supplemented nutrient broth ( ) in 2-liter flasks (at 30°C and 200 rpm) supplemented with 1 μg/ml erythromycin to provide selective pressure for maintenance of the plasmid-borne receptor operon.

    Binding Assay:

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Paragraph title: 2.6. Binding of MBP to microbial cells, insoluble PGs and curdlan ... Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

    SDS Page:

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Following 10% SDS-PAGE and electro-transfer, immunoblot analysis was performed using 1:3000 diluted MBP antiserum as the first antibody. .. Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

    Plasmid Preparation:

    Article Title: Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor ▿
    Article Snippet: .. Escherichia coli strains used for SDM (XL1-Blue and XL10-Gold [Stratagene]) or preparation of plasmid for transformation of B. megaterium ( E. coli NovaBlue [Novagen]) were cultured in LB medium at 37°C supplemented with 75 μg/ml carbenicillin. .. B . megaterium spores were prepared in 200-ml aliquots of supplemented nutrient broth ( ) in 2-liter flasks (at 30°C and 200 rpm) supplemented with 1 μg/ml erythromycin to provide selective pressure for maintenance of the plasmid-borne receptor operon.

    Article Title: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3′-Hydroxylation Using Recombinant Escherichia coli
    Article Snippet: .. Construction of Recombinant E. coli Expressing Tyrosinase from B. megaterium The tyrosinase gene from B. megaterium was amplified by polymerase chain reaction (PCR) and subcloned into pETDuet-1TM (Novagen, Madison, WI, USA) to form the expression vector pETDuet-BmTYR ( a). .. The recombinant E. coli harboring the expression vector was confirmed to produce considerable tyrosinase protein (36 kDa) after isopropyl-β-d -thiogalactopyranoside (IPTG) induction ( b).

    Software:

    Article Title: Recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ?-1,3-glucanase-related protein in Manduca sexta larval plasma
    Article Snippet: Intensities (I) of MBP bands in the bound and unbound fractions were quantified using Kodak Digital Analysis Software to estimate the binding extent: 4 × Ibound ÷ (4 × Ibound + 5 × Iunbound ). .. Similarly, insoluble DAP-PGs from E. coli K12 (0.5 mg, InvivoGen), B. megaterium (2.0 mg) and B. subtilis (2.0 mg), insoluble Lys-PGs from M. luteus and S. aureus (2.0 mg each) , and insoluble β-1,3-glucan from Alcaligenes faecalis ( i.e . curdlan, 2.0 mg, Sigma-Aldrich) were mixed at 4 C for 1 h with 0.5 μg MBP in 50 μl of buffer D containing benzamidine and PTU.

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    Millipore b megaterium mj1212
    Beneficial Effect of B. <t>megaterium</t> <t>mj1212</t> on Plant Growth
    B Megaterium Mj1212, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b megaterium mj1212/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b megaterium mj1212 - by Bioz Stars, 2020-03
    80/100 stars
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    Beneficial Effect of B. megaterium mj1212 on Plant Growth

    Journal: Indian Journal of Microbiology

    Article Title: Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

    doi: 10.1007/s12088-014-0476-6

    Figure Lengend Snippet: Beneficial Effect of B. megaterium mj1212 on Plant Growth

    Article Snippet: To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35 °C, and filtreted through 0.22 μm Millipore filter and 10 μl of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 × 300 mm) at 40 °C.

    Techniques:

    Effect of B. megaterium mj1212 application on plant amino acids

    Journal: Indian Journal of Microbiology

    Article Title: Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

    doi: 10.1007/s12088-014-0476-6

    Figure Lengend Snippet: Effect of B. megaterium mj1212 application on plant amino acids

    Article Snippet: To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35 °C, and filtreted through 0.22 μm Millipore filter and 10 μl of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 × 300 mm) at 40 °C.

    Techniques:

    Phosphate solubilization ability of Bacillus megaterium mj1212 on National Botanical Research Institute’s Phosphate (NBRIP) media plates

    Journal: Indian Journal of Microbiology

    Article Title: Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

    doi: 10.1007/s12088-014-0476-6

    Figure Lengend Snippet: Phosphate solubilization ability of Bacillus megaterium mj1212 on National Botanical Research Institute’s Phosphate (NBRIP) media plates

    Article Snippet: To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35 °C, and filtreted through 0.22 μm Millipore filter and 10 μl of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 × 300 mm) at 40 °C.

    Techniques:

    Influence of B. megaterium mj1212 on soluble sugar content in mustard leaves

    Journal: Indian Journal of Microbiology

    Article Title: Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

    doi: 10.1007/s12088-014-0476-6

    Figure Lengend Snippet: Influence of B. megaterium mj1212 on soluble sugar content in mustard leaves

    Article Snippet: To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35 °C, and filtreted through 0.22 μm Millipore filter and 10 μl of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 × 300 mm) at 40 °C.

    Techniques:

    HPLC analysis of organic acids in culture filtrate of B. megaterium mj1212

    Journal: Indian Journal of Microbiology

    Article Title: Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth

    doi: 10.1007/s12088-014-0476-6

    Figure Lengend Snippet: HPLC analysis of organic acids in culture filtrate of B. megaterium mj1212

    Article Snippet: To determine the organic acids production in culture medium, B. megaterium mj1212 was inoculated into tricalcium phosphate medium (pH 7.0) and cultured at 35 °C, and filtreted through 0.22 μm Millipore filter and 10 μl of filtrates were injected to High Performance Liquid Chromatography (Model: Waters 600E) equipped with a Refractive Index Detector (Model: Waters 410) and RSpak KC-811 column (8.0 × 300 mm) at 40 °C.

    Techniques: High Performance Liquid Chromatography