cholera toxin b conjugated to alexa fluor 555  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cholera toxin b conjugated to alexa fluor 555
    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with <t>Alexa</t> Fluor <t>555-labeled</t> CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P
    Cholera Toxin B Conjugated To Alexa Fluor 555, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cholera toxin b conjugated to alexa fluor 555/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cholera toxin b conjugated to alexa fluor 555 - by Bioz Stars, 2022-11
    95/100 stars

    Images

    1) Product Images from "TPC1 Has Two Variant Isoforms, and Their Removal Has Different Effects on Endo-Lysosomal Functions Compared to Loss of TPC2"

    Article Title: TPC1 Has Two Variant Isoforms, and Their Removal Has Different Effects on Endo-Lysosomal Functions Compared to Loss of TPC2

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.00113-14

    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with Alexa Fluor 555-labeled CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P
    Figure Legend Snippet: Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with Alexa Fluor 555-labeled CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P

    Techniques Used: Knock-Out, Expressing, Incubation, Labeling, Immunolabeling, Marker, Binding Assay, shRNA, Sequencing, Quantitative RT-PCR

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    Thermo Fisher cholera toxin b conjugated to alexa fluor 555
    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with <t>Alexa</t> Fluor <t>555-labeled</t> CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P
    Cholera Toxin B Conjugated To Alexa Fluor 555, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cholera toxin b conjugated to alexa fluor 555/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cholera toxin b conjugated to alexa fluor 555 - by Bioz Stars, 2022-11
    95/100 stars
      Buy from Supplier

    96
    Thermo Fisher buffer b 1
    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with <t>Alexa</t> Fluor <t>555-labeled</t> CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P
    Buffer B 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer b 1/product/Thermo Fisher
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    94
    Thermo Fisher kif 23 fp b
    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with <t>Alexa</t> Fluor <t>555-labeled</t> CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P
    Kif 23 Fp B, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with Alexa Fluor 555-labeled CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P

    Journal: Molecular and Cellular Biology

    Article Title: TPC1 Has Two Variant Isoforms, and Their Removal Has Different Effects on Endo-Lysosomal Functions Compared to Loss of TPC2

    doi: 10.1128/MCB.00113-14

    Figure Lengend Snippet: Impact of knockout expression of Tpcn1A/B or Tpcn2 in cholera toxin trafficking. (A) Schematic representation of CTxB trafficking from the plasma membrane to the Golgi apparatus. (B) Representative images for 0-min (no chase) and 120-min chase periods at 37°C, after 30 min incubation on ice with Alexa Fluor 555-labeled CTxB. Cells were immunolabeled with an antibody against the Golgi apparatus marker protein GM130. Cell boundaries are identified with a white broken line. (C) Quantification of CTxB levels in the Golgi apparatus at the indicated chase time after the CTxB binding period ( n = 56 to 93). (D, E) Effects of treatment of WT MEFs with shRNA (shRNA targeting Rab7b or a scrambled sequence control) on relative Rab7b mRNA levels determined by RT-qPCR (D) and on CTxB levels in the Golgi apparatus after a chase period of 120 min (E) ( n = 32 to 36). Data points correspond to the mean ± SEM. ns, no significant difference ( P > 0.05); **, P

    Article Snippet: Primary MEFs grown on poly-d-lysine-treated coverslips were incubated on ice for 30 min in Hanks balanced salt solution buffer (Sigma) containing 1 μg/ml Alexa Fluor 555-labeled subunit B of cholera toxin (CTxB; Invitrogen).

    Techniques: Knock-Out, Expressing, Incubation, Labeling, Immunolabeling, Marker, Binding Assay, shRNA, Sequencing, Quantitative RT-PCR