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biotinylated lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated lycopersicon esculentum tomato lectin
    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
    Biotinylated Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+1175/Biotinylated+Lycopersicon+Esculentum+(Tomato)+Lectin+(LEL%2C+TL)/pmc12893876-310-8-13
    Average 95 stars, based on 431 article reviews
    biotinylated lycopersicon esculentum tomato lectin - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation"

    Article Title: Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation

    Journal: Journal of Materials Chemistry. B

    doi: 10.1039/d5tb02179k

    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
    Figure Legend Snippet: Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Techniques Used: Staining

    Related Articles

    other:

    Article Title: Reactive microglia and IL1β/IL-1R1-signaling mediate neuroprotection in excitotoxin-damaged mouse retina
    Article Snippet: Tomato Lectin , 1:1000 , n/a , B-1175 , Vector Laboratories.

    Article Title: Development of a paediatric model of diffuse traumatic brain injury in ferrets
    Article Snippet: Tomato Lectin (Lycopersicon esculentum; 1:1000, Vector Laboratories cat# B-1175, RRID: AB_2315475) , Streptavidin Alexa fluor 488 conjugate (Thermo Fisher, Cat# S11223 , No RRID) , Round 2 488 nm.

    Article Title: Neuroprotective effects of SMTP-44D in mice stroke model in relation to neurovascular unit and trophic coupling.
    Article Snippet: Then calculated the difference value TA B L E 1 Primary antibodies used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used TNF‐α Escherichia coli‐derived recombinant mouse TNF‐alpha R and D Systems, goat polyclonal, AF‐410‐NA, AB_354479 1:100 Iba‐1 Synthetic peptide corresponding to C‐terminus of Iba1 Wako, rabbit polyclonal, 019‐19741, AB_839504 1:1,000 Cleaved caspase‐3 Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino‐termi‐ nal residues adjacent to (Asp175) in human caspase‐3 Cell Signaling Technology, rabbit polyclonal, 9661, AB_2341188 1:300 Nitrotyrosine Nitrated klh (keyhole limpet hemocyanin) Sigma, rabbit polyclonal, N0409, AB_260745 1:200 RAGE Synthetic peptide, (C)WRKRQP(R/L) EERKAP ESQED(N), corresp. to aa 362‐380 of rat RAGE Abcam, rabbit polyclonal, ab3611, AB_303947 1:200 4HNE Synthetic 4‐Hydroxynonenal modified Keyhole Limpet Kemocyanin (KLH) JalCA, mouse monoclonal, MHN‐100P, AB_1106813 1:50 8‐OHdG 8‐hydroxy‐2′‐deoxyguanosine is produced by reactive oxygen and nitrogen species, including hydroxyl radical and peroxynitrite JalCA, mouse monoclonal, MOG‐100P, AB_1106818 1:20 LEL LEL binds well to glycophorin and Tamm‐Horsfall glycoprotein Vector Laboratories, B‐1175, AB_2315475 1:200 NG2 HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592‐2222 Millipore, mouse monoclonal, 05‐710, AB_309925 1:1,000 GFAP Purified glial filament Abcam, goat polyclonal, ab53554, AB_880202 1:1,000 Collagen IV Full length native protein (extracted and purified from tumor tissues) (Mouse) Abcam, rabbit polyclonal, ab19808, AB_445160 1:500 BDNF A synthetic peptide from human mature BDNF conjugated to an immunogenic carrier protein Abcam, sheep polyclonal, ab75040, AB_1280756 1:100 TrkB A peptide mapping within a C‐terminalcy‐ toplasmic domain of Trk B of mouse origin Santa Cruz Biotechnology, rabbit polyclonal, sc‐8316, AB_2155274 1:100 NeuN Purified cell nuclei from mouse brain Millipore, mouse monoclonal, MAB377, AB_2298772 1:200 before and after the operation.

    Concentration Assay:

    Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2.
    Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, B-1175, AB_2315475 1:200 GST-p Purified human glutathione S-transferase pi MBL, rabbit polyclonal, 312, AB_591792 1:500 GFAP GFAP isolated from cow spinal cord Dako, rabbit polyclonal, Z0334, AB_10013382 1:1,000 Iba-1 Synthetic peptide corresponding to C- terminus of Iba1 Wako, rabbit polyclonal, 019-19741, AB_839504 1:1,000 Journal of Neuroscience Research Fig. 3. ..

    Recombinant:

    Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2.
    Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, B-1175, AB_2315475 1:200 GST-p Purified human glutathione S-transferase pi MBL, rabbit polyclonal, 312, AB_591792 1:500 GFAP GFAP isolated from cow spinal cord Dako, rabbit polyclonal, Z0334, AB_10013382 1:1,000 Iba-1 Synthetic peptide corresponding to C- terminus of Iba1 Wako, rabbit polyclonal, 019-19741, AB_839504 1:1,000 Journal of Neuroscience Research Fig. 3. ..

    Purification:

    Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2.
    Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, B-1175, AB_2315475 1:200 GST-p Purified human glutathione S-transferase pi MBL, rabbit polyclonal, 312, AB_591792 1:500 GFAP GFAP isolated from cow spinal cord Dako, rabbit polyclonal, Z0334, AB_10013382 1:1,000 Iba-1 Synthetic peptide corresponding to C- terminus of Iba1 Wako, rabbit polyclonal, 019-19741, AB_839504 1:1,000 Journal of Neuroscience Research Fig. 3. ..

    Isolation:

    Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2.
    Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, B-1175, AB_2315475 1:200 GST-p Purified human glutathione S-transferase pi MBL, rabbit polyclonal, 312, AB_591792 1:500 GFAP GFAP isolated from cow spinal cord Dako, rabbit polyclonal, Z0334, AB_10013382 1:1,000 Iba-1 Synthetic peptide corresponding to C- terminus of Iba1 Wako, rabbit polyclonal, 019-19741, AB_839504 1:1,000 Journal of Neuroscience Research Fig. 3. ..

    Immunofluorescence:

    Article Title: Nicotinamide riboside rescues angiotensin II–induced cerebral small vessel disease in mice
    Article Snippet: The nuclei were counterstained with 4′‐6‐diamidino‐2‐phenylindole (DAPI; Santa Cruz Biotechnology). .. To visualize brain blood vessels, biotinylated Lycopersicon esculentum (tomato) lectin (LEL, TL) (1:200; Vector Laboratories, B‐1175) was used to label endothelial cells, and the experimental procedure was the same as that for immunofluorescence. .. The sections were then incubated with DyLight 488–conjugated Streptavidin (1:200; Vector Laboratories, SA‐5488) overnight at 4°C.



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    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
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    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
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    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
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    Image Search Results


    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Journal: Journal of Materials Chemistry. B

    Article Title: Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation

    doi: 10.1039/d5tb02179k

    Figure Lengend Snippet: Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Article Snippet: Under anesthesia, a 50 μL retro-orbital injection of biotinylated Lycopersicon esculentum (tomato) Lectin (Vector Labs, Cat. No. B-1175-1) was administered and allowed to circulate for 10 minutes.

    Techniques: Staining