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Axygen axyprep mag pcr clean up beads
Axyprep Mag Pcr Clean Up Beads, supplied by Axygen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axyprep mag pcr clean up beads/product/Axygen
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
axyprep mag pcr clean up beads - by Bioz Stars, 2020-09
90/100 stars

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Flow Cytometry:

Article Title: Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial
Article Snippet: .. Amplicons were purified using AxyPrep Mag PCR clean-up beads (Axygen; Corning, NY) quantified with Quanti-iT PicoGreen,dsDNA Assay Kit (Invitrogen; Eugene, OR), and pooled in equimolar ratios prior to sequencing at the Colorado State University Genomics Core facility using a 2 × 250 MiSeq flow cell (Illumina, San Diego, CA) , . .. Paired-end sequence reads were concatenated and all combined 16s sequences were filtered, trimmed and processed with the DADA2 (R bioconductor package) ).

Amplification:

Article Title: The Metabolic Response to a Low Amino Acid Diet is Independent of Diet-Induced Shifts in the Composition of the Gut Microbiome
Article Snippet: .. Following initial amplification, reactions were cleaned using a 0.7x volume of AxyPrep Mag PCR clean-up beads (Axygen Biosciences, Union City, CA). .. In a subsequent PCR, Illumina dual indexes and Sequencing adapters were added using the following primers (Forward primer: 5′-AATGATACGGCGACCACCGAGATCTACAC[55555555]ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′, Reverse Primer: 5′-CAAGCAGAAGACGGCATACGAGAT[77777777] G TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′, where bracketed sequences are equivalent to the Illumina Dual Index adapters D501-D508 and D701-D712,N716,N718-N724,N726-N729).

Picogreen Assay:

Article Title: Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial
Article Snippet: .. Amplicons were purified using AxyPrep Mag PCR clean-up beads (Axygen; Corning, NY) quantified with Quanti-iT PicoGreen,dsDNA Assay Kit (Invitrogen; Eugene, OR), and pooled in equimolar ratios prior to sequencing at the Colorado State University Genomics Core facility using a 2 × 250 MiSeq flow cell (Illumina, San Diego, CA) , . .. Paired-end sequence reads were concatenated and all combined 16s sequences were filtered, trimmed and processed with the DADA2 (R bioconductor package) ).

Purification:

Article Title: A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
Article Snippet: .. The reactions were purified using 90 μl of AxyPrep Mag PCR Clean-Up beads with two 70% ethanol washes (450 μl) and elution with 24 μl of nuclease-free water. .. The final libraries were quantified by Qubit double-stranded DNA high sensitivity assay and analysed electrophoretically on a Bioanalyser high sensitivity DNA chip.

Article Title: Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial
Article Snippet: .. Amplicons were purified using AxyPrep Mag PCR clean-up beads (Axygen; Corning, NY) quantified with Quanti-iT PicoGreen,dsDNA Assay Kit (Invitrogen; Eugene, OR), and pooled in equimolar ratios prior to sequencing at the Colorado State University Genomics Core facility using a 2 × 250 MiSeq flow cell (Illumina, San Diego, CA) , . .. Paired-end sequence reads were concatenated and all combined 16s sequences were filtered, trimmed and processed with the DADA2 (R bioconductor package) ).

Article Title: The landscape of miRNA editing in animals and its impact on miRNA biogenesis and targeting
Article Snippet: .. Preamplified product was purified using Axygen AxyPrep Mag PCR clean-up beads. .. Preamplified cDNAs and primer pools were loaded into the 48.48 Access Array IFC (Fluidigm), primed, mixed, amplified, and harvested.

Article Title: A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
Article Snippet: .. During the bisulphite treatment (2.5 h at 64 °C, followed by 4 °C hold), the other, neutralised aliquot was purified using 1.8 × AxyPrep Mag PCR Clean-Up beads. ..

Polymerase Chain Reaction:

Article Title: The Metabolic Response to a Low Amino Acid Diet is Independent of Diet-Induced Shifts in the Composition of the Gut Microbiome
Article Snippet: .. Following initial amplification, reactions were cleaned using a 0.7x volume of AxyPrep Mag PCR clean-up beads (Axygen Biosciences, Union City, CA). .. In a subsequent PCR, Illumina dual indexes and Sequencing adapters were added using the following primers (Forward primer: 5′-AATGATACGGCGACCACCGAGATCTACAC[55555555]ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′, Reverse Primer: 5′-CAAGCAGAAGACGGCATACGAGAT[77777777] G TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′, where bracketed sequences are equivalent to the Illumina Dual Index adapters D501-D508 and D701-D712,N716,N718-N724,N726-N729).

Article Title: A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
Article Snippet: .. The reactions were purified using 90 μl of AxyPrep Mag PCR Clean-Up beads with two 70% ethanol washes (450 μl) and elution with 24 μl of nuclease-free water. .. The final libraries were quantified by Qubit double-stranded DNA high sensitivity assay and analysed electrophoretically on a Bioanalyser high sensitivity DNA chip.

Article Title: Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial
Article Snippet: .. Amplicons were purified using AxyPrep Mag PCR clean-up beads (Axygen; Corning, NY) quantified with Quanti-iT PicoGreen,dsDNA Assay Kit (Invitrogen; Eugene, OR), and pooled in equimolar ratios prior to sequencing at the Colorado State University Genomics Core facility using a 2 × 250 MiSeq flow cell (Illumina, San Diego, CA) , . .. Paired-end sequence reads were concatenated and all combined 16s sequences were filtered, trimmed and processed with the DADA2 (R bioconductor package) ).

Article Title: The landscape of miRNA editing in animals and its impact on miRNA biogenesis and targeting
Article Snippet: .. Preamplified product was purified using Axygen AxyPrep Mag PCR clean-up beads. .. Preamplified cDNAs and primer pools were loaded into the 48.48 Access Array IFC (Fluidigm), primed, mixed, amplified, and harvested.

Article Title: The Metabolic Response to a Low Amino Acid Diet is Independent of Diet-Induced Shifts in the Composition of the Gut Microbiome
Article Snippet: .. Following PCR, reactions were cleaned using a 0.7x volume of AxyPrep Mag PCR clean-up beads (Axygen Biosciences). .. Quality and quantity of the finished libraries were assessed using an Agilent DNA 1000 kit (Agilent Technologies, Santa Clara, CA) and Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific), respectively.

Article Title: Effect of Sweetened Dried Cranberry Consumption on Urinary Proteome and Fecal Microbiome in Healthy Human Subjects
Article Snippet: .. Following PCR, samples were cleaned using a 1x volume of AxyPrep Mag PCR clean-up beads (Axygen Biosciences). .. Quality and quantity of the finished libraries were assessed using an Agilent DNA1000 chip and Qubit dsDNA HS Assay Kit, respectively.

Article Title: A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample
Article Snippet: .. During the bisulphite treatment (2.5 h at 64 °C, followed by 4 °C hold), the other, neutralised aliquot was purified using 1.8 × AxyPrep Mag PCR Clean-Up beads. ..

Sequencing:

Article Title: Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial
Article Snippet: .. Amplicons were purified using AxyPrep Mag PCR clean-up beads (Axygen; Corning, NY) quantified with Quanti-iT PicoGreen,dsDNA Assay Kit (Invitrogen; Eugene, OR), and pooled in equimolar ratios prior to sequencing at the Colorado State University Genomics Core facility using a 2 × 250 MiSeq flow cell (Illumina, San Diego, CA) , . .. Paired-end sequence reads were concatenated and all combined 16s sequences were filtered, trimmed and processed with the DADA2 (R bioconductor package) ).

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    Axygen axyprep mag pcr clean up beads
    Axyprep Mag Pcr Clean Up Beads, supplied by Axygen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep mag pcr clean up beads/product/Axygen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    axyprep mag pcr clean up beads - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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