Structured Review

Carl Zeiss axiovert 200m microscope
In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss <t>Axiovert</t> <t>200M</t> microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P
Axiovert 200m Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 2063 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axiovert 200m microscope/product/Carl Zeiss
Average 95 stars, based on 2063 article reviews
Price from $9.99 to $1999.99
axiovert 200m microscope - by Bioz Stars, 2020-08
95/100 stars

Images

1) Product Images from "The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP"

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2018.00320

In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P
Figure Legend Snippet: In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

Techniques Used: In Vitro, Mutagenesis, Infection, Staining, Microscopy

2) Product Images from "Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT"

Article Title: Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT

Journal: Scientific Reports

doi: 10.1038/srep07850

CDTa-specific nanobody l+8 and CDTb-specific nanobody l-15.1g inhibit CDTa+b induced cytotoxicity. Before addition to cultures of adherent HT29 cells, CDTa and CDTb were preincubated with a 16 fold excess of CDTa- or CDTb-specific nanobodies. Four hours after addition of toxins, cells were fixed and cellular morphology was assessed by differential interference microscopy. The cellular cytoskeleton was stained with the F-actin-staining dye phalloidin-rhodamine, cell nuclei were stained with the DNA staining dye Hoechst 33342. Apotome-images were captured with a Zeiss Axiovert 200M microscope. Control cells (top) were incubated for four hours in the absence or presence of CDTa+b.
Figure Legend Snippet: CDTa-specific nanobody l+8 and CDTb-specific nanobody l-15.1g inhibit CDTa+b induced cytotoxicity. Before addition to cultures of adherent HT29 cells, CDTa and CDTb were preincubated with a 16 fold excess of CDTa- or CDTb-specific nanobodies. Four hours after addition of toxins, cells were fixed and cellular morphology was assessed by differential interference microscopy. The cellular cytoskeleton was stained with the F-actin-staining dye phalloidin-rhodamine, cell nuclei were stained with the DNA staining dye Hoechst 33342. Apotome-images were captured with a Zeiss Axiovert 200M microscope. Control cells (top) were incubated for four hours in the absence or presence of CDTa+b.

Techniques Used: Microscopy, Staining, Incubation

3) Product Images from "Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated heterozygous deletion"

Article Title: Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated heterozygous deletion

Journal: bioRxiv

doi: 10.1101/2020.05.13.093914

LeishIF4E2(+/-) mutant cells easily transform to axenic amastigote-like cells. (A) Promastigotes of the LeishIF4E2(+/-) mutant, control wild type, and Cas9/T7 expressing cells grown under normal conditions are shown. (B) Morphology of cells transferred to conditions that induce differentiation to axenic-amastigotes (33°C/pH 5.5) during four days. Images were captured at100x magnification with a Zeiss Axiovert 200M microscope equipped with AxioCam HRm CCD camera. The scale bar is 10 µm. (C) Upregulated proteins in the mutant LeishIF4E2(+/-) promastigotes (compared to WT cells) and in published amastigotes proteome. The total protein of the LeishIF4E2(+/-) mutant promastigotes was compared to the proteome of WT cells. The list of upregulated proteins was further compared with the proteins enriched in the amastigote proteome of virulent L. amazonensis PH8 strain (de Rezende et al, PLOS NTD 2017). and of L. mexicana amastigotes (Paape et al, Mol Cell Prot 2008).
Figure Legend Snippet: LeishIF4E2(+/-) mutant cells easily transform to axenic amastigote-like cells. (A) Promastigotes of the LeishIF4E2(+/-) mutant, control wild type, and Cas9/T7 expressing cells grown under normal conditions are shown. (B) Morphology of cells transferred to conditions that induce differentiation to axenic-amastigotes (33°C/pH 5.5) during four days. Images were captured at100x magnification with a Zeiss Axiovert 200M microscope equipped with AxioCam HRm CCD camera. The scale bar is 10 µm. (C) Upregulated proteins in the mutant LeishIF4E2(+/-) promastigotes (compared to WT cells) and in published amastigotes proteome. The total protein of the LeishIF4E2(+/-) mutant promastigotes was compared to the proteome of WT cells. The list of upregulated proteins was further compared with the proteins enriched in the amastigote proteome of virulent L. amazonensis PH8 strain (de Rezende et al, PLOS NTD 2017). and of L. mexicana amastigotes (Paape et al, Mol Cell Prot 2008).

Techniques Used: Mutagenesis, Expressing, Microscopy

4) Product Images from "Heterogeneous distribution of Candida albicans cell-surface antigens demonstrated with an Als1-specific monoclonal antibody"

Article Title: Heterogeneous distribution of Candida albicans cell-surface antigens demonstrated with an Als1-specific monoclonal antibody

Journal: Microbiology

doi: 10.1099/mic.0.043851-0

Photomicrographs of Alexa Fluor 594-labelled and anti-Als1-labelled C. albicans yeast cells to demonstrate decreasing cell-surface Als1 abundance on successive generations of yeast culture growth as well as stability of Als1 over time. Cells from a saturated YPD culture of strain CAI12 were transferred to fresh medium and grown at 30 °C for the indicated times [0 h, (a); 1 h, (b); 4 h, (c); 24 h, (d)]. In (a–c), replicate photomicrographs of the same image are shown illuminated with individual or combinations of wavelengths of light, as indicated. A Zeiss Axiovert 200M microscope was used to image cells. Anti-Als1 mAb was detected by a FITC-conjugated secondary antibody and 488 nm light (488). Alexa 594 covalently linked to the C. albicans cell surface was detected with 594 nm light (594). Bright-field microscopy was used to visualize all cells in a field (Br). Als1-negative buds are indicated by white arrowheads in (c). The photomicrograph in (d) was illuminated with all three light sources and shows many Als1-negative yeasts and a rare dual-labelled Als1/Alexa 594-positive cell (asterisk). Als1-positive cells in (d) are indicated by black arrows. Faint outlines of cells were the result of either autofluorescence (green) or bleeding of the FITC signal into the 594 channel (purple). Together, the images show that Als1-positive cells were rare in saturated cultures, that transfer of cells to fresh medium produced a coating of Als1, and that the protein coat persisted on the cell surface as the culture aged. (e) Real-time RT-PCR quantification of ALS1 transcriptional activity over the course of culture growth to demonstrate the burst in transcription that occurs following inoculation into fresh culture medium and the decreasing abundance of ALS1 RNA as culture growth progresses. Data from a representative biological replicate of the experiment are shown; triplicate data points were gathered for each time point. The threshold value ( C t ) is graphed so that lower values indicate greater RNA abundance. (f) False-coloured image from a replicate time-course experiment viewed with a Zeiss LSM 710 NLO system. False colouring with a gradient of colour emphasizes the difference in anti-Als1 labelling intensity between the inoculum yeast (Alexa 594-positive, purple; Als1-positive, cyan gradient) and its daughter (Als1-positive, cyan gradient). Decreased anti-Als1 labelling intensity suggests that the transcriptional burst occurs in the inoculum cell and is diluted by cell division in each subsequent generation until anti-Als1 labelling intensity falls below the limit of detection for the assay.
Figure Legend Snippet: Photomicrographs of Alexa Fluor 594-labelled and anti-Als1-labelled C. albicans yeast cells to demonstrate decreasing cell-surface Als1 abundance on successive generations of yeast culture growth as well as stability of Als1 over time. Cells from a saturated YPD culture of strain CAI12 were transferred to fresh medium and grown at 30 °C for the indicated times [0 h, (a); 1 h, (b); 4 h, (c); 24 h, (d)]. In (a–c), replicate photomicrographs of the same image are shown illuminated with individual or combinations of wavelengths of light, as indicated. A Zeiss Axiovert 200M microscope was used to image cells. Anti-Als1 mAb was detected by a FITC-conjugated secondary antibody and 488 nm light (488). Alexa 594 covalently linked to the C. albicans cell surface was detected with 594 nm light (594). Bright-field microscopy was used to visualize all cells in a field (Br). Als1-negative buds are indicated by white arrowheads in (c). The photomicrograph in (d) was illuminated with all three light sources and shows many Als1-negative yeasts and a rare dual-labelled Als1/Alexa 594-positive cell (asterisk). Als1-positive cells in (d) are indicated by black arrows. Faint outlines of cells were the result of either autofluorescence (green) or bleeding of the FITC signal into the 594 channel (purple). Together, the images show that Als1-positive cells were rare in saturated cultures, that transfer of cells to fresh medium produced a coating of Als1, and that the protein coat persisted on the cell surface as the culture aged. (e) Real-time RT-PCR quantification of ALS1 transcriptional activity over the course of culture growth to demonstrate the burst in transcription that occurs following inoculation into fresh culture medium and the decreasing abundance of ALS1 RNA as culture growth progresses. Data from a representative biological replicate of the experiment are shown; triplicate data points were gathered for each time point. The threshold value ( C t ) is graphed so that lower values indicate greater RNA abundance. (f) False-coloured image from a replicate time-course experiment viewed with a Zeiss LSM 710 NLO system. False colouring with a gradient of colour emphasizes the difference in anti-Als1 labelling intensity between the inoculum yeast (Alexa 594-positive, purple; Als1-positive, cyan gradient) and its daughter (Als1-positive, cyan gradient). Decreased anti-Als1 labelling intensity suggests that the transcriptional burst occurs in the inoculum cell and is diluted by cell division in each subsequent generation until anti-Als1 labelling intensity falls below the limit of detection for the assay.

Techniques Used: Microscopy, Produced, Quantitative RT-PCR, Activity Assay

5) Product Images from "Therapeutic potential of Focal Adhesion Kinase inhibition in small cell lung cancer"

Article Title: Therapeutic potential of Focal Adhesion Kinase inhibition in small cell lung cancer

Journal: Molecular cancer therapeutics

doi: 10.1158/1535-7163.MCT-18-0328

PF-228’s effect on migration and invasion in SCLC cell lines. A. Migration evaluation by wound healing assay associated with time-lapse microscopy. Two adherent SCLC cell lines were grown to confluence, wounded, incubated overnight with culture medium, and then treated with PF-228 or DMSO for 12h. Cells were monitored during these 12h using a Zeiss Axiovert 200M microscope (Zeiss, Thornwood, NY). Images were captured every 15min. Velocity of cell migration was measured using ImageJ. Decreased motility was observed in cell lines treated with PF-228 as compared to those treated with DMSO. Error bars represent mean +/− SD from three independent experiments. B. Invasion evaluation by modified Boyden Chamber assay. Two adherent SCLC cell lines (one adherent and one with mixed-morphology) were seeded on the top of an insert pre-coated with matrigel and separating the two chambers of a transwell. Culture medium containing 1%-FBS was placed in the upper chamber and 10%-FBS in the lower chamber. After 12h-treatment with PF-228 or DMSO, cells that moved through the pores towards the bottom of the insert were stained with crystal violet, digitally pictured, and quantified by the free software ImageJ (NIH, Bethesda, MD, USA). Right panels are pictures of SCLC cell lines stained with crystal violet on the lower side of the insert which are representative of the numerous fields (x10 magnification) analyzed in two independent experiments performed in duplicate wells. Left panels represent quantification of the number of cells per field on the bottom of the insert. Decreased invasion was observed in cell lines treated with PF-228 for 12h as compared to those treated with DMSO. Error bars represent mean +/− SD from two independent experiments.
Figure Legend Snippet: PF-228’s effect on migration and invasion in SCLC cell lines. A. Migration evaluation by wound healing assay associated with time-lapse microscopy. Two adherent SCLC cell lines were grown to confluence, wounded, incubated overnight with culture medium, and then treated with PF-228 or DMSO for 12h. Cells were monitored during these 12h using a Zeiss Axiovert 200M microscope (Zeiss, Thornwood, NY). Images were captured every 15min. Velocity of cell migration was measured using ImageJ. Decreased motility was observed in cell lines treated with PF-228 as compared to those treated with DMSO. Error bars represent mean +/− SD from three independent experiments. B. Invasion evaluation by modified Boyden Chamber assay. Two adherent SCLC cell lines (one adherent and one with mixed-morphology) were seeded on the top of an insert pre-coated with matrigel and separating the two chambers of a transwell. Culture medium containing 1%-FBS was placed in the upper chamber and 10%-FBS in the lower chamber. After 12h-treatment with PF-228 or DMSO, cells that moved through the pores towards the bottom of the insert were stained with crystal violet, digitally pictured, and quantified by the free software ImageJ (NIH, Bethesda, MD, USA). Right panels are pictures of SCLC cell lines stained with crystal violet on the lower side of the insert which are representative of the numerous fields (x10 magnification) analyzed in two independent experiments performed in duplicate wells. Left panels represent quantification of the number of cells per field on the bottom of the insert. Decreased invasion was observed in cell lines treated with PF-228 for 12h as compared to those treated with DMSO. Error bars represent mean +/− SD from two independent experiments.

Techniques Used: Migration, Wound Healing Assay, Time-lapse Microscopy, Incubation, Microscopy, Modification, Boyden Chamber Assay, Staining, Software

6) Product Images from "A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB"

Article Title: A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB

Journal: PLoS ONE

doi: 10.1371/journal.pone.0011672

Delayed infection kinetics of the ORF4 − Tet + and ORF4.STOP mutants as determined by fluorescence microscopy. For immunofluorescence staining, NIH3T3 cells were seeded on coverslips in 12 well plates the day before staining. Cells were infected at a multiplicity of infection of 5–10 for 1h at 4°C to allow adsorption. Then, cells were washed three times and were either immediately (0h – upper panel) fixed with 4% paraformaldehyde or prewarmed medium was added and the cells were further incubated at 37°C. Three hours later (3h – median panel) or eight hours later (8h – lower panel), cells were fixed and permeabilized with 0.1% Triton-X100. Virus particles were visualized with polyclonal rabbit anti-MHV-68 antiserum followed by goat anti-rabbitCy3 antibody. Stained cells were mounted in ProLong® Gold antifade reagent with DAPI and analysed by using a Zeiss Axiovert 200M microscope. Cells were viewed with a 100× oil immersion objective. The top panel shows staining of uninfected cells as control. One representative experiment of 5 is shown.
Figure Legend Snippet: Delayed infection kinetics of the ORF4 − Tet + and ORF4.STOP mutants as determined by fluorescence microscopy. For immunofluorescence staining, NIH3T3 cells were seeded on coverslips in 12 well plates the day before staining. Cells were infected at a multiplicity of infection of 5–10 for 1h at 4°C to allow adsorption. Then, cells were washed three times and were either immediately (0h – upper panel) fixed with 4% paraformaldehyde or prewarmed medium was added and the cells were further incubated at 37°C. Three hours later (3h – median panel) or eight hours later (8h – lower panel), cells were fixed and permeabilized with 0.1% Triton-X100. Virus particles were visualized with polyclonal rabbit anti-MHV-68 antiserum followed by goat anti-rabbitCy3 antibody. Stained cells were mounted in ProLong® Gold antifade reagent with DAPI and analysed by using a Zeiss Axiovert 200M microscope. Cells were viewed with a 100× oil immersion objective. The top panel shows staining of uninfected cells as control. One representative experiment of 5 is shown.

Techniques Used: Infection, Fluorescence, Microscopy, Immunofluorescence, Staining, Adsorption, Incubation

7) Product Images from "IL17: potential therapeutic target in Sj?gren's syndrome using adenovirus-mediated gene transfer"

Article Title: IL17: potential therapeutic target in Sj?gren's syndrome using adenovirus-mediated gene transfer

Journal: Laboratory investigation; a journal of technical methods and pathology

doi: 10.1038/labinvest.2010.164

Histological analyses of salivary glands Examination of the salivary glands in mice cannulated at 8 wks or indicated “Early Treatment” (n=15) ( A-F ) or 17 wks of age or indicated “Late Treatment” (n=18) ( G-L ) with either Ad5-LacZ or Ad5-IL17R:Fc vectors at 10 7 viral particles per gland. . Black arrows indicate representative lymphocytic infiltrate in H E sections ( A, D, G J ), immunofluorescent staining for CD3 + T and B220+B cells ( B H) ) and immunohistochemical staining for IL17 cells ( C, E, I K ). Isotype control for IL-17 antibody was done with rabbit IgG ( F L ). Images were taken at 200× magnification at constant exposure of 0.3 second using Zeiss Axiovert 200M microscope (Carl Zeiss, Thornwood, NY).
Figure Legend Snippet: Histological analyses of salivary glands Examination of the salivary glands in mice cannulated at 8 wks or indicated “Early Treatment” (n=15) ( A-F ) or 17 wks of age or indicated “Late Treatment” (n=18) ( G-L ) with either Ad5-LacZ or Ad5-IL17R:Fc vectors at 10 7 viral particles per gland. . Black arrows indicate representative lymphocytic infiltrate in H E sections ( A, D, G J ), immunofluorescent staining for CD3 + T and B220+B cells ( B H) ) and immunohistochemical staining for IL17 cells ( C, E, I K ). Isotype control for IL-17 antibody was done with rabbit IgG ( F L ). Images were taken at 200× magnification at constant exposure of 0.3 second using Zeiss Axiovert 200M microscope (Carl Zeiss, Thornwood, NY).

Techniques Used: Mouse Assay, Staining, Immunohistochemistry, Microscopy

8) Product Images from "Immunostaining and time-lapse analysis of vinblastine-induced paracrystal formation in human A549 cells"

Article Title: Immunostaining and time-lapse analysis of vinblastine-induced paracrystal formation in human A549 cells

Journal: Oncology Letters

doi: 10.3892/ol.2014.2549

Paracrystal formation in human A549 cells. A549 cells were treated with a vinblastine solution and fixed. The cells were stained with an anti-tubulin antibody followed by an appropriate secondary antibody. Nuclei were stained with DAPI. Images were acquired using an Axiovert 200M microscope. Bar = 10 μm.
Figure Legend Snippet: Paracrystal formation in human A549 cells. A549 cells were treated with a vinblastine solution and fixed. The cells were stained with an anti-tubulin antibody followed by an appropriate secondary antibody. Nuclei were stained with DAPI. Images were acquired using an Axiovert 200M microscope. Bar = 10 μm.

Techniques Used: Staining, Microscopy

Overlapping RFP-Tubulin immunostaining. RFP-Tubulin was introduced into the A549 cells, which were immunostained with either an anti-tubulin antibody or an anti-RBM8A antibody and appropriate secondary antibodies conjugated with Alexa 488. Images were acquired using an Axiovert 200M microscope. Bar = 10 μm.
Figure Legend Snippet: Overlapping RFP-Tubulin immunostaining. RFP-Tubulin was introduced into the A549 cells, which were immunostained with either an anti-tubulin antibody or an anti-RBM8A antibody and appropriate secondary antibodies conjugated with Alexa 488. Images were acquired using an Axiovert 200M microscope. Bar = 10 μm.

Techniques Used: Immunostaining, Microscopy

9) Product Images from "The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP"

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2018.00320

In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P
Figure Legend Snippet: In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

Techniques Used: In Vitro, Mutagenesis, Infection, Staining, Microscopy

10) Product Images from "The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP"

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2018.00320

In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P
Figure Legend Snippet: In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

Techniques Used: In Vitro, Mutagenesis, Infection, Staining, Microscopy

11) Product Images from "Distant Cytosolic Residues Mediate a Two-way Molecular Switch That Controls the Modulation of Inwardly Rectifying Potassium (Kir) Channels by Cholesterol and Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2) *"

Article Title: Distant Cytosolic Residues Mediate a Two-way Molecular Switch That Controls the Modulation of Inwardly Rectifying Potassium (Kir) Channels by Cholesterol and Phosphatidylinositol 4,5-Bisphosphate (PI(4,5)P2) *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M111.336339

A , images of HEK293 cells transfected with HA-Kir2.1 and its L222I, N251D, and L222I/N251D mutants viewed using a Zeiss AxioVert 200M microscope. B , current-voltage curves obtained from single channel recordings of Kir2.1 and its L222I, N251D, and L222I/N251D mutants. The conductance of each construct, which was calculated from the slope of the linear fit of the currents recorded between −100 and −10 mV, is displayed in the figure. C , representative traces of single channel recordings obtained from Kir2.1 and its L222I, N251D, and L222I/N251D mutants recorded at −80 mV under a cell-attached mode. D , open probability of the channel and its L222I, N251D, and L222I/N251D mutants obtained from single channel recordings. Significant differences are indicated by asterisks (*, p ≤ 0.05). E , mean peak normalized currents for HEK293 cell populations co-transfected with enhanced GFP and HA-Kir2.1 or its L222I, N251D, and L222I/N251D mutants ( n = 18–68). Error bars represent S.E.
Figure Legend Snippet: A , images of HEK293 cells transfected with HA-Kir2.1 and its L222I, N251D, and L222I/N251D mutants viewed using a Zeiss AxioVert 200M microscope. B , current-voltage curves obtained from single channel recordings of Kir2.1 and its L222I, N251D, and L222I/N251D mutants. The conductance of each construct, which was calculated from the slope of the linear fit of the currents recorded between −100 and −10 mV, is displayed in the figure. C , representative traces of single channel recordings obtained from Kir2.1 and its L222I, N251D, and L222I/N251D mutants recorded at −80 mV under a cell-attached mode. D , open probability of the channel and its L222I, N251D, and L222I/N251D mutants obtained from single channel recordings. Significant differences are indicated by asterisks (*, p ≤ 0.05). E , mean peak normalized currents for HEK293 cell populations co-transfected with enhanced GFP and HA-Kir2.1 or its L222I, N251D, and L222I/N251D mutants ( n = 18–68). Error bars represent S.E.

Techniques Used: Transfection, Microscopy, Construct

12) Product Images from "Autoantibodies against Muscarinic Type 3 Receptor in Sj?gren's Syndrome Inhibit Aquaporin 5 Trafficking"

Article Title: Autoantibodies against Muscarinic Type 3 Receptor in Sj?gren's Syndrome Inhibit Aquaporin 5 Trafficking

Journal: PLoS ONE

doi: 10.1371/journal.pone.0053113

Inhibition of hAQP5 trafficking in rhAQP5 vector-transfected HSG cells in the presence of 4-DAMP. (A) rhAQP5 vector-transfected HSG cells were stimulated with CCh (100 μM) in the presence or absence of 4-DAMP (10 μM), prenzepine (10 μM), and mixture of 4-DAMP and prenzepine (M3R or M1R specific antagonist, respectively). (B) To monitor hAQP5 trafficking, original GFP pictures in (A) were converted into ‘Fire’ 3D LUT (lookup tables) setting in ImageJ software and only GFP signal above the fluorescent signal threshold (≥80, red or yellow color in converted picture), was captured in (B) as positive GFP signal. Yellow arrows indicate the positive GFP signal on the membrane and green arrows indicates signal in the cytoplasm in HSG cells. Cells were observed at ×200 magnification using a Zeiss Axiovert 200M microscope and images were obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).
Figure Legend Snippet: Inhibition of hAQP5 trafficking in rhAQP5 vector-transfected HSG cells in the presence of 4-DAMP. (A) rhAQP5 vector-transfected HSG cells were stimulated with CCh (100 μM) in the presence or absence of 4-DAMP (10 μM), prenzepine (10 μM), and mixture of 4-DAMP and prenzepine (M3R or M1R specific antagonist, respectively). (B) To monitor hAQP5 trafficking, original GFP pictures in (A) were converted into ‘Fire’ 3D LUT (lookup tables) setting in ImageJ software and only GFP signal above the fluorescent signal threshold (≥80, red or yellow color in converted picture), was captured in (B) as positive GFP signal. Yellow arrows indicate the positive GFP signal on the membrane and green arrows indicates signal in the cytoplasm in HSG cells. Cells were observed at ×200 magnification using a Zeiss Axiovert 200M microscope and images were obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).

Techniques Used: Inhibition, Plasmid Preparation, Transfection, Software, Microscopy

Presence of anti-M3R autoantibodies in SjS plasma detected by immunocytochemistry. (A) HSG cells were pre-incubated with (a) healthy control (HC) plasma, (b, e) Sjögren's syndrome (SjS) plasma, (c) pre-absorbed SjS plasma, or (d) IgG depleted SjS plasma for 24 hours. Endogenous expressions of M3R (red color) on HSG cells were detected by using poly- (a–d) or mono-(e) clonal antibody. Immunofluorescence staining of HSG cells following pre-incubation with SjS plasma indicated co-localization [yellow or orange color in merged picture (b) and (e)] of human IgG (green color) and M3R (red color) on HSG cells. This specific binding was confirmed by pre-absorbed (c), IgG depleted plasma (d), or monoclonal antibody specific for M3R (e). Stained cells were observed at a ×200 magnification. (pM3R* Ab: polyclonal anti-hM3R antibody; mM3R Ab: monoclonal anti-hM3R antibody). (B) HSG cells were incubated with purified IgG from five pooled-SjS plasma samples and polyclonal anti-M3R antibody followed by Alexa Fluor 488 and 568-labeled secondary antibody incubation, respectively. Yellow (b, c, d, e, and h) or white arrows (a, f, and g) in the image picture indicate co-localization or adjacent staining of IgG and M3R, respectively. poly α-M3R ab, polyclonal antibody; mono α-M3R ab, monoclonal antibody; Green arrows, IgG staining; red arrows, M3R staining; white arrows, M3R and IgG adjacent staining; yellow arrows, co-localization of IgG and M3R. Cells were observed at ×400 magnification using a Zeiss Axiovert 200M microscope and images were obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).
Figure Legend Snippet: Presence of anti-M3R autoantibodies in SjS plasma detected by immunocytochemistry. (A) HSG cells were pre-incubated with (a) healthy control (HC) plasma, (b, e) Sjögren's syndrome (SjS) plasma, (c) pre-absorbed SjS plasma, or (d) IgG depleted SjS plasma for 24 hours. Endogenous expressions of M3R (red color) on HSG cells were detected by using poly- (a–d) or mono-(e) clonal antibody. Immunofluorescence staining of HSG cells following pre-incubation with SjS plasma indicated co-localization [yellow or orange color in merged picture (b) and (e)] of human IgG (green color) and M3R (red color) on HSG cells. This specific binding was confirmed by pre-absorbed (c), IgG depleted plasma (d), or monoclonal antibody specific for M3R (e). Stained cells were observed at a ×200 magnification. (pM3R* Ab: polyclonal anti-hM3R antibody; mM3R Ab: monoclonal anti-hM3R antibody). (B) HSG cells were incubated with purified IgG from five pooled-SjS plasma samples and polyclonal anti-M3R antibody followed by Alexa Fluor 488 and 568-labeled secondary antibody incubation, respectively. Yellow (b, c, d, e, and h) or white arrows (a, f, and g) in the image picture indicate co-localization or adjacent staining of IgG and M3R, respectively. poly α-M3R ab, polyclonal antibody; mono α-M3R ab, monoclonal antibody; Green arrows, IgG staining; red arrows, M3R staining; white arrows, M3R and IgG adjacent staining; yellow arrows, co-localization of IgG and M3R. Cells were observed at ×400 magnification using a Zeiss Axiovert 200M microscope and images were obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).

Techniques Used: Immunocytochemistry, Incubation, Immunofluorescence, Staining, Binding Assay, Purification, Labeling, Microscopy, Software

Increased membrane expression of GFP-tagged-recombinant human aquaporin 5 ( rhAQP5 ) following carbachol stimulation in HSG cells. (A) M3R expression was detected in HSG cells (red color) using polyclonal (a, b) or monoclonal (c) antibody by immunocytochemistry. Stained cells were observed at ×200 (a) and ×400 (b, c) magnifications. (B) hAQP5 protein expressions were examined by western blotting in total HSG cell lysates purified from non- or rhAQP5 vector-transfected HSG cells (a) and in HSG cell membrane fractions purified from transfected cells with or without CCh treatment (b) in duplicates #1 and #2. (C) To confirm the hAQP5 trafficking in HSG cells, control GFP (a) or rhAQP5 (c) vector transfected HSG cells were treated with carbachol (CCh, 100 μM) (a and c) or no stimulation (b). Images were taken under microscope with a ×400 magnification using a Zeiss Axiovert 200M microscope and obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).
Figure Legend Snippet: Increased membrane expression of GFP-tagged-recombinant human aquaporin 5 ( rhAQP5 ) following carbachol stimulation in HSG cells. (A) M3R expression was detected in HSG cells (red color) using polyclonal (a, b) or monoclonal (c) antibody by immunocytochemistry. Stained cells were observed at ×200 (a) and ×400 (b, c) magnifications. (B) hAQP5 protein expressions were examined by western blotting in total HSG cell lysates purified from non- or rhAQP5 vector-transfected HSG cells (a) and in HSG cell membrane fractions purified from transfected cells with or without CCh treatment (b) in duplicates #1 and #2. (C) To confirm the hAQP5 trafficking in HSG cells, control GFP (a) or rhAQP5 (c) vector transfected HSG cells were treated with carbachol (CCh, 100 μM) (a and c) or no stimulation (b). Images were taken under microscope with a ×400 magnification using a Zeiss Axiovert 200M microscope and obtained with AxioVs40 software (Ver. 4.7.1.0, Zeiss).

Techniques Used: Expressing, Recombinant, Immunocytochemistry, Staining, Western Blot, Purification, Plasmid Preparation, Transfection, Microscopy, Software

13) Product Images from "The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP"

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP

Journal: Frontiers in Cellular and Infection Microbiology

doi: 10.3389/fcimb.2018.00320

In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P
Figure Legend Snippet: In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

Techniques Used: In Vitro, Mutagenesis, Infection, Staining, Microscopy

14) Product Images from "LeishIF4E1 Deletion Affects the Promastigote Proteome, Morphology, and Infectivity"

Article Title: LeishIF4E1 Deletion Affects the Promastigote Proteome, Morphology, and Infectivity

Journal: mSphere

doi: 10.1128/mSphere.00625-19

The LeishIF4E1 –/– null mutant cells are impaired in their ability to dedifferentiate from amastigote-like cells to promastigotes. Promastigotes of the LeishIF4E1 –/– null mutant, wild-type control, Cas9/T7-expressing cells, and LeishIF4E1 add-back cells are shown in the top row. These cells were transferred to conditions known to induce differentiation to axenic amastigotes by their transfer to growth at 33°C and pH 5.5 for 4 days (second row from top). All cell lines were then transferred back to 25°C and pH 7.4 to allow their transformation back to promastigotes. Following this switch, the cells were monitored on a daily basis (rows 3 to 6 from the top). Images were captured at a ×100 magnification with a Zeiss Axiovert 200M microscope equipped with an AxioCam HRm CCD camera. Scale bar, 2 μm.
Figure Legend Snippet: The LeishIF4E1 –/– null mutant cells are impaired in their ability to dedifferentiate from amastigote-like cells to promastigotes. Promastigotes of the LeishIF4E1 –/– null mutant, wild-type control, Cas9/T7-expressing cells, and LeishIF4E1 add-back cells are shown in the top row. These cells were transferred to conditions known to induce differentiation to axenic amastigotes by their transfer to growth at 33°C and pH 5.5 for 4 days (second row from top). All cell lines were then transferred back to 25°C and pH 7.4 to allow their transformation back to promastigotes. Following this switch, the cells were monitored on a daily basis (rows 3 to 6 from the top). Images were captured at a ×100 magnification with a Zeiss Axiovert 200M microscope equipped with an AxioCam HRm CCD camera. Scale bar, 2 μm.

Techniques Used: Mutagenesis, Expressing, Transformation Assay, Microscopy

15) Product Images from "Vaccinia virus temperature-sensitive mutants in the A28 gene produce non-infectious virions that bind to cells but are defective in entry"

Article Title: Vaccinia virus temperature-sensitive mutants in the A28 gene produce non-infectious virions that bind to cells but are defective in entry

Journal: Virology

doi: 10.1016/j.virol.2007.03.060

Binding of Cts9 virions to cells but lack of entry The upper four panels show binding to CV-1 cells of purified mature virions of either WT VV or Cts9 grown at 31°C or 40°C. CV-1 cells were infected at a multiplicity of 10 (for WT VV or Cts9 grown at 31°C), or for Cts9 grown at 40°C with an equivalent number of virus particles. Following adsorption of virions to cells for 1h at room temperature, the cells were fixed and reacted with anti-A27 mAb followed by goat anti-mouse FITC secondary Ab. The virions were pseudocolored cyan. Nuclei were counterstained by propidium iodide (red). The lower four panels show the result of the virus entry assay. Mature virions were adsorbed to cells at RT, and the cells transferred to 31°C for 2h to allow virus entry to occur. The cells were fixed, permeabilized, and stained with rabbit anti-A4 serum followed by goat anti-rabbit FITC (green) to visualize intracellular cores. Counterstaining of nuclei was with propidium iodide. The cells were photographed with a Zeiss Axiovert 200M inverted fluorescence microscope. Arrows in the lower panels indicate representative core particles.
Figure Legend Snippet: Binding of Cts9 virions to cells but lack of entry The upper four panels show binding to CV-1 cells of purified mature virions of either WT VV or Cts9 grown at 31°C or 40°C. CV-1 cells were infected at a multiplicity of 10 (for WT VV or Cts9 grown at 31°C), or for Cts9 grown at 40°C with an equivalent number of virus particles. Following adsorption of virions to cells for 1h at room temperature, the cells were fixed and reacted with anti-A27 mAb followed by goat anti-mouse FITC secondary Ab. The virions were pseudocolored cyan. Nuclei were counterstained by propidium iodide (red). The lower four panels show the result of the virus entry assay. Mature virions were adsorbed to cells at RT, and the cells transferred to 31°C for 2h to allow virus entry to occur. The cells were fixed, permeabilized, and stained with rabbit anti-A4 serum followed by goat anti-rabbit FITC (green) to visualize intracellular cores. Counterstaining of nuclei was with propidium iodide. The cells were photographed with a Zeiss Axiovert 200M inverted fluorescence microscope. Arrows in the lower panels indicate representative core particles.

Techniques Used: Binding Assay, Purification, Infection, Adsorption, Staining, Fluorescence, Microscopy

16) Product Images from "LeishIF4E1 Deletion Affects the Promastigote Proteome, Morphology, and Infectivity"

Article Title: LeishIF4E1 Deletion Affects the Promastigote Proteome, Morphology, and Infectivity

Journal: mSphere

doi: 10.1128/mSphere.00625-19

The LeishIF4E1 –/– null mutant cells are impaired in their ability to dedifferentiate from amastigote-like cells to promastigotes. Promastigotes of the LeishIF4E1 –/– null mutant, wild-type control, Cas9/T7-expressing cells, and LeishIF4E1 add-back cells are shown in the top row. These cells were transferred to conditions known to induce differentiation to axenic amastigotes by their transfer to growth at 33°C and pH 5.5 for 4 days (second row from top). All cell lines were then transferred back to 25°C and pH 7.4 to allow their transformation back to promastigotes. Following this switch, the cells were monitored on a daily basis (rows 3 to 6 from the top). Images were captured at a ×100 magnification with a Zeiss Axiovert 200M microscope equipped with an AxioCam HRm CCD camera. Scale bar, 2 μm.
Figure Legend Snippet: The LeishIF4E1 –/– null mutant cells are impaired in their ability to dedifferentiate from amastigote-like cells to promastigotes. Promastigotes of the LeishIF4E1 –/– null mutant, wild-type control, Cas9/T7-expressing cells, and LeishIF4E1 add-back cells are shown in the top row. These cells were transferred to conditions known to induce differentiation to axenic amastigotes by their transfer to growth at 33°C and pH 5.5 for 4 days (second row from top). All cell lines were then transferred back to 25°C and pH 7.4 to allow their transformation back to promastigotes. Following this switch, the cells were monitored on a daily basis (rows 3 to 6 from the top). Images were captured at a ×100 magnification with a Zeiss Axiovert 200M microscope equipped with an AxioCam HRm CCD camera. Scale bar, 2 μm.

Techniques Used: Mutagenesis, Expressing, Transformation Assay, Microscopy

Related Articles

Staining:

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP
Article Snippet: .. The samples were stained with anti- Citrobacter LPS rabbit polyclonal antibody in PBS containing 0.2% Tween 20 and 3% BSA at 4°C for 3 h. Following the primary antibody, the samples were incubated with anti-rabbit Alexa 488 secondary antibody and DAPI in PBS containing 0.2% Tween 20 and 3% BSA at 37°C for 1 h. Finally, the samples were mounted in Prolong Gold, and imaged on a Zeiss Axiovert 200M microscope with a Zeiss Axiocam monochrome camera. .. Bacteria were cultured overnight in LB broth, 220 rpm, at 37°C.

Article Title: A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB
Article Snippet: .. Stained cells were mounted in ProLong® Gold antifade reagent with DAPI (Molecular Probes Inc., Eugene, OR) and analysed by using a Zeiss Axiovert 200M microscope. ..

Incubation:

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP
Article Snippet: .. The samples were stained with anti- Citrobacter LPS rabbit polyclonal antibody in PBS containing 0.2% Tween 20 and 3% BSA at 4°C for 3 h. Following the primary antibody, the samples were incubated with anti-rabbit Alexa 488 secondary antibody and DAPI in PBS containing 0.2% Tween 20 and 3% BSA at 37°C for 1 h. Finally, the samples were mounted in Prolong Gold, and imaged on a Zeiss Axiovert 200M microscope with a Zeiss Axiocam monochrome camera. .. Bacteria were cultured overnight in LB broth, 220 rpm, at 37°C.

Microscopy:

Article Title: Immunostaining and time-lapse analysis of vinblastine-induced paracrystal formation in human A549 cells
Article Snippet: .. Images were acquired using an Axiovert 200M microscope (Carl Zeiss, Oberkochen, Germany) and processed using ZEN software (Carl Zeiss). .. Vinblastine-induced paracrystal formation in A549 cells To confirm paracrystal formation in the A549 cells, the cells were treated with vinblastine for 12 h and the paracrystals that formed were stained with an anti-tubulin antibody, as described in previous studies ( , ).

Article Title: Heterogeneous distribution of Candida albicans cell-surface antigens demonstrated with an Als1-specific monoclonal antibody
Article Snippet: .. The second was a Zeiss Axiovert 200M microscope equipped with a mercury X-Cite illuminator, an ApoTome Structured Illumination Optical Sectioning system with a VH ApoTome grid under a weak ApoTome filter setting, standard excitation and emission filters for FITC and rhodamine, a Plan-Apochromat ×63/1.40 numerical aperture oil objective lens, and an AxioCam MRc 5 colour camera. .. The third microscope was a Zeiss LSM 710 NLO confocal scanner, Axio Observer Z1 microscope and a Spectraphysics Mai Tai Ti : Sapphire laser.

Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP
Article Snippet: .. The samples were stained with anti- Citrobacter LPS rabbit polyclonal antibody in PBS containing 0.2% Tween 20 and 3% BSA at 4°C for 3 h. Following the primary antibody, the samples were incubated with anti-rabbit Alexa 488 secondary antibody and DAPI in PBS containing 0.2% Tween 20 and 3% BSA at 37°C for 1 h. Finally, the samples were mounted in Prolong Gold, and imaged on a Zeiss Axiovert 200M microscope with a Zeiss Axiocam monochrome camera. .. Bacteria were cultured overnight in LB broth, 220 rpm, at 37°C.

Article Title: Selection of Nanobodies that Block the Enzymatic and Cytotoxic Activities of the Binary Clostridium Difficile Toxin CDT
Article Snippet: .. Fixed cells were counterstained with rhodamine-phalloidin and Hoechst 33342 (Molecular Probes, Life Technologies, Carlsbad, CA), and analyzed by microscopy with a Zeiss Axiovert 200M microscope equipped with digital interference contrast and an apotome (Zeiss, Oberkochen, Germany). .. Additional cytotoxicity assays were carried out by monitoring the transepithelial resistance (TEER) of confluent monolayers of MDCK C7 cells grown on 0.4 µM filter-plates with a Volt Ohm meter (Merck Millipore, Billerica, MA, USA).

Article Title: Therapeutic potential of Focal Adhesion Kinase inhibition in small cell lung cancer
Article Snippet: .. Cell movements within wounded area were monitored for 12h starting from the time drug was added using a Zeiss Axiovert 200M microscope (Zeiss, Thornwood, NY) at x200 magnification. ..

Article Title: IL17: potential therapeutic target in Sj?gren's syndrome using adenovirus-mediated gene transfer
Article Snippet: .. After three washes, fluorescence was detected by fluorescence microscopy at 200× magnification using a Zeiss Axiovert 200M microscope and all images were obtained with AxioVs40 software with constant exposure of 0.3 seconds (Carl Zeiss, Thornwood, NY). .. Statistical analyses Statistical evaluations between saliva collections were determined by using Mann-Whitney U test generated by the GraphPad InStat software (GraphPad Software, La Jolla, CA).

Article Title: Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated heterozygous deletion
Article Snippet: .. Phase contrast microscope images were captured at X100 magnification with a Zeiss Axiovert 200M microscope equipped with AxioCam HRm CCD camera. .. Flow cytometry analysis of Leishmania Cell viability was verified by incubation of the cells with 20 µg/mL propidium iodide (PI) during 30 minutes.

Article Title: A Gammaherpesvirus Complement Regulatory Protein Promotes Initiation of Infection by Activation of Protein Kinase Akt/PKB
Article Snippet: .. Stained cells were mounted in ProLong® Gold antifade reagent with DAPI (Molecular Probes Inc., Eugene, OR) and analysed by using a Zeiss Axiovert 200M microscope. ..

Fluorescence:

Article Title: IL17: potential therapeutic target in Sj?gren's syndrome using adenovirus-mediated gene transfer
Article Snippet: .. After three washes, fluorescence was detected by fluorescence microscopy at 200× magnification using a Zeiss Axiovert 200M microscope and all images were obtained with AxioVs40 software with constant exposure of 0.3 seconds (Carl Zeiss, Thornwood, NY). .. Statistical analyses Statistical evaluations between saliva collections were determined by using Mann-Whitney U test generated by the GraphPad InStat software (GraphPad Software, La Jolla, CA).

Software:

Article Title: Immunostaining and time-lapse analysis of vinblastine-induced paracrystal formation in human A549 cells
Article Snippet: .. Images were acquired using an Axiovert 200M microscope (Carl Zeiss, Oberkochen, Germany) and processed using ZEN software (Carl Zeiss). .. Vinblastine-induced paracrystal formation in A549 cells To confirm paracrystal formation in the A549 cells, the cells were treated with vinblastine for 12 h and the paracrystals that formed were stained with an anti-tubulin antibody, as described in previous studies ( , ).

Article Title: IL17: potential therapeutic target in Sj?gren's syndrome using adenovirus-mediated gene transfer
Article Snippet: .. After three washes, fluorescence was detected by fluorescence microscopy at 200× magnification using a Zeiss Axiovert 200M microscope and all images were obtained with AxioVs40 software with constant exposure of 0.3 seconds (Carl Zeiss, Thornwood, NY). .. Statistical analyses Statistical evaluations between saliva collections were determined by using Mann-Whitney U test generated by the GraphPad InStat software (GraphPad Software, La Jolla, CA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Carl Zeiss axiovert 200m microscope system
    Immunofluorescence Staining of Stably Transfected HL60 Cells Reveals Retrocyclin Peptides (A) Retrocyclin peptides RC-100, RC-101, and RC-101_2K peptides (in duplicates) and (B) RC-100, RC-100b, RC-101, protegrin-1 (PG-1), rhesus theta defensin-1 (RTD-1), and human neutrophil peptides 1–3 (HNP 1–3) were dotted (0–8 ng/4 μl dot) on a PVDF membrane and subjected to immuno-dotblot analysis. (C) VC, R1R3, and A1A3 (100,000 cells each) were fixed onto glass slides and incubated with rabbit anti-RC-101 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody and then stained using fluorescein isothiocyanate (FITC)-avidin. Slides were visualized using Zeiss <t>Axiovert</t> <t>200M</t> microscope system at 40× magnification. The three rows show FITC staining, DIC, and the merged image, respectively. Scale bar represents 20 μm.
    Axiovert 200m Microscope System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 85/100, based on 2067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axiovert 200m microscope system/product/Carl Zeiss
    Average 85 stars, based on 2067 article reviews
    Price from $9.99 to $1999.99
    axiovert 200m microscope system - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Carl Zeiss axiovert 200m inverted fluorescence microscope
    AQP5 expression in MDCK cells. (A-B) Epifluorescent images show the subcellular localization of AQP5 in live MDCK cells. Fluorescence is shown in inverted contrast. (A) Untreated MDCK AQP5-EGFP cells. (B) Untreated MDCK AQP5-myc-EGFP cells labeled with QDs. Insets (boxed areas) highlight the homogenous plasma membrane distribution of AQP5 in the flat portion of the cell. To compare cellular localization, AQP5-EGFP was acquired with 100 ms integration, whereas AQP5-myc-EGFP was acquired with 20 ms integration on a Zeiss <t>Axiovert</t> <t>200M</t> inverted epifluorescence microscope. EGFP images were adjusted to the same minimum and maximum displayed intensity values. Scale bars are 20 μm and 7 μm ( insets ).
    Axiovert 200m Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axiovert 200m inverted fluorescence microscope/product/Carl Zeiss
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    axiovert 200m inverted fluorescence microscope - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Immunofluorescence Staining of Stably Transfected HL60 Cells Reveals Retrocyclin Peptides (A) Retrocyclin peptides RC-100, RC-101, and RC-101_2K peptides (in duplicates) and (B) RC-100, RC-100b, RC-101, protegrin-1 (PG-1), rhesus theta defensin-1 (RTD-1), and human neutrophil peptides 1–3 (HNP 1–3) were dotted (0–8 ng/4 μl dot) on a PVDF membrane and subjected to immuno-dotblot analysis. (C) VC, R1R3, and A1A3 (100,000 cells each) were fixed onto glass slides and incubated with rabbit anti-RC-101 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody and then stained using fluorescein isothiocyanate (FITC)-avidin. Slides were visualized using Zeiss Axiovert 200M microscope system at 40× magnification. The three rows show FITC staining, DIC, and the merged image, respectively. Scale bar represents 20 μm.

    Journal: PLoS Biology

    Article Title: Reawakening Retrocyclins: Ancestral Human Defensins Active Against HIV-1Rousing a Latent Defense Mechanism to Fight HIV

    doi: 10.1371/journal.pbio.1000095

    Figure Lengend Snippet: Immunofluorescence Staining of Stably Transfected HL60 Cells Reveals Retrocyclin Peptides (A) Retrocyclin peptides RC-100, RC-101, and RC-101_2K peptides (in duplicates) and (B) RC-100, RC-100b, RC-101, protegrin-1 (PG-1), rhesus theta defensin-1 (RTD-1), and human neutrophil peptides 1–3 (HNP 1–3) were dotted (0–8 ng/4 μl dot) on a PVDF membrane and subjected to immuno-dotblot analysis. (C) VC, R1R3, and A1A3 (100,000 cells each) were fixed onto glass slides and incubated with rabbit anti-RC-101 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody and then stained using fluorescein isothiocyanate (FITC)-avidin. Slides were visualized using Zeiss Axiovert 200M microscope system at 40× magnification. The three rows show FITC staining, DIC, and the merged image, respectively. Scale bar represents 20 μm.

    Article Snippet: The slides were then visualized on a Zeiss Axiovert 200M microscope system with 450 ms exposure time for all slides.

    Techniques: Immunofluorescence, Staining, Stable Transfection, Transfection, Incubation, Avidin-Biotin Assay, Microscopy

    In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Virulence Effect of CpxRA in Citrobacter rodentium Is Independent of the Auxiliary Proteins NlpE and CpxP

    doi: 10.3389/fcimb.2018.00320

    Figure Lengend Snippet: In vitro adherence of wild-type and Cpx TCS mutant strains of Citrobacter rodentium on HeLa cells. HeLa cells were infected with wild-type C. rodentium or a Cpx TCS mutant strain for 8 hrs. The samples were fixed and stained with DAPI and anti- Citrobacter LPS. Samples were imaged on a Zeiss Axiovert 200M microscope. Total number of bacteria per HeLa cell were counted. The 3 biological replicates for each strain are color-coded. Each replicate consists of 10 fields of view. A non-parametric one-way ANOVA was used to determine statistical significance (**** P

    Article Snippet: The samples were stained with anti- Citrobacter LPS rabbit polyclonal antibody in PBS containing 0.2% Tween 20 and 3% BSA at 4°C for 3 h. Following the primary antibody, the samples were incubated with anti-rabbit Alexa 488 secondary antibody and DAPI in PBS containing 0.2% Tween 20 and 3% BSA at 37°C for 1 h. Finally, the samples were mounted in Prolong Gold, and imaged on a Zeiss Axiovert 200M microscope with a Zeiss Axiocam monochrome camera.

    Techniques: In Vitro, Mutagenesis, Infection, Staining, Microscopy

    AQP5 expression in MDCK cells. (A-B) Epifluorescent images show the subcellular localization of AQP5 in live MDCK cells. Fluorescence is shown in inverted contrast. (A) Untreated MDCK AQP5-EGFP cells. (B) Untreated MDCK AQP5-myc-EGFP cells labeled with QDs. Insets (boxed areas) highlight the homogenous plasma membrane distribution of AQP5 in the flat portion of the cell. To compare cellular localization, AQP5-EGFP was acquired with 100 ms integration, whereas AQP5-myc-EGFP was acquired with 20 ms integration on a Zeiss Axiovert 200M inverted epifluorescence microscope. EGFP images were adjusted to the same minimum and maximum displayed intensity values. Scale bars are 20 μm and 7 μm ( insets ).

    Journal: PLoS ONE

    Article Title: Opposing Effects of cAMP and T259 Phosphorylation on Plasma Membrane Diffusion of the Water Channel Aquaporin-5 in Madin-Darby Canine Kidney Cells

    doi: 10.1371/journal.pone.0133324

    Figure Lengend Snippet: AQP5 expression in MDCK cells. (A-B) Epifluorescent images show the subcellular localization of AQP5 in live MDCK cells. Fluorescence is shown in inverted contrast. (A) Untreated MDCK AQP5-EGFP cells. (B) Untreated MDCK AQP5-myc-EGFP cells labeled with QDs. Insets (boxed areas) highlight the homogenous plasma membrane distribution of AQP5 in the flat portion of the cell. To compare cellular localization, AQP5-EGFP was acquired with 100 ms integration, whereas AQP5-myc-EGFP was acquired with 20 ms integration on a Zeiss Axiovert 200M inverted epifluorescence microscope. EGFP images were adjusted to the same minimum and maximum displayed intensity values. Scale bars are 20 μm and 7 μm ( insets ).

    Article Snippet: Time-lapse microscopy Imaging of QD-labeled AQP5-myc-EGFP was performed on a Zeiss Axiovert 200M inverted fluorescence microscope equipped with a Xenon lamp and a 37°C heated stage using a 63x/1.45 NA oil objective (Carl Zeiss, Jena, Germany).

    Techniques: Expressing, Fluorescence, Labeling, Mass Spectrometry, Inverted Epifluorescence