Structured Review

Carl Zeiss axiophot 2 microscope
Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss <t>Axiophot</t> 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.
Axiophot 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axiophot 2 microscope/product/Carl Zeiss
Average 92 stars, based on 37 article reviews
Price from $9.99 to $1999.99
axiophot 2 microscope - by Bioz Stars, 2020-08
92/100 stars

Images

1) Product Images from "Thyroid Hormone Signaling in the Mouse Retina"

Article Title: Thyroid Hormone Signaling in the Mouse Retina

Journal: PLoS ONE

doi: 10.1371/journal.pone.0168003

Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss Axiophot 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.
Figure Legend Snippet: Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss Axiophot 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.

Techniques Used: Mouse Assay, Staining, Microscopy

Higher power micrographs from wholemounted FINDT3 retinae stained with X-gal. For the adult mouse (left), the GCL and INL are shown separately as they are on different focal planes. For the p10 mouse (right) only the GCL is shown. Dorsal and ventral fields are from far peripheral retina. The scale bar applies to all images. Images were acquired with a Zeiss Axiophot 2 microscope.
Figure Legend Snippet: Higher power micrographs from wholemounted FINDT3 retinae stained with X-gal. For the adult mouse (left), the GCL and INL are shown separately as they are on different focal planes. For the p10 mouse (right) only the GCL is shown. Dorsal and ventral fields are from far peripheral retina. The scale bar applies to all images. Images were acquired with a Zeiss Axiophot 2 microscope.

Techniques Used: Staining, Microscopy

Vertical cryostat sections of FINDT3 and wildtype mouse retinae stained for β-galactosidase. Top row: X-gal staining, blue dots indicate β-gal-positive cells. In adult FINDT3 mice (left) staining occurs in the ganglion cell layer (GCL) and inner nuclear layer (INL), but not in the outer nuclear layer (ONL). In FINDT3 mice at postnatal day 10 (p10, middle) staining mainly occurs in the GCL with only few labeled cells in the INL. There is no staining in the adult wildtype control (WT, right). Bottom row: Staining with the mouse monoclonal antibody against β-gal. As in the X-gal staining, adult FINDT3 mice show two bands of stained cells in the GCL and INL, respectively. At p10 staining mainly occurs in the GCL with few labeled cells in the INL. In all these immunolabeled sections, blood vessels are also stained by the anti-mouse secondary antibody (some arrowed); in the wildtype control, the only labeled structures are blood vessels. OPL, outer plexiform layer; IPL, inner plexiform layer. Images were acquired with a Zeiss Axiophot 2 microscope (top row) and a Zeiss Axioplan 2 microscope (bottom row). The scale bar applies to all images.
Figure Legend Snippet: Vertical cryostat sections of FINDT3 and wildtype mouse retinae stained for β-galactosidase. Top row: X-gal staining, blue dots indicate β-gal-positive cells. In adult FINDT3 mice (left) staining occurs in the ganglion cell layer (GCL) and inner nuclear layer (INL), but not in the outer nuclear layer (ONL). In FINDT3 mice at postnatal day 10 (p10, middle) staining mainly occurs in the GCL with only few labeled cells in the INL. There is no staining in the adult wildtype control (WT, right). Bottom row: Staining with the mouse monoclonal antibody against β-gal. As in the X-gal staining, adult FINDT3 mice show two bands of stained cells in the GCL and INL, respectively. At p10 staining mainly occurs in the GCL with few labeled cells in the INL. In all these immunolabeled sections, blood vessels are also stained by the anti-mouse secondary antibody (some arrowed); in the wildtype control, the only labeled structures are blood vessels. OPL, outer plexiform layer; IPL, inner plexiform layer. Images were acquired with a Zeiss Axiophot 2 microscope (top row) and a Zeiss Axioplan 2 microscope (bottom row). The scale bar applies to all images.

Techniques Used: Staining, Mouse Assay, Labeling, Immunolabeling, Microscopy

Related Articles

TUNEL Assay:

Article Title: Rapid Transgene Expression in Multiple Precursor Cell Types of Adult Rat Subventricular Zone Mediated by Adeno-Associated Type 1 Vectors
Article Snippet: .. TUNEL-positive cells were quantified with a Zeiss Axiophot 2 microscope equipped with fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) filters (Carl Zeiss, Göttingen, Germany) on five sections surrounding the injection site, covering 400 μm along the anteroposterior axis. .. Cells were counted in the SVZ ipsilateral and contralateral to the injection site.

Fluorescence:

Article Title: Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B
Article Snippet: .. Digital fluorescence microscopic images were acquired using an Axiophot 2 microscope (Zeiss, Oberkochen, Germany) equipped with a CCD camera (Visitron Systems, Puchheim, Germany) and afterwards processed with Photoshop CS3 (Adobe, San Jose, CA, USA). .. IgG fluorescence intensity in the endoneurium of femoral quadriceps nerve cross sections was measured with ImageJ (NIH, Bethesda, Maryland).

Labeling:

Article Title: Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways
Article Snippet: .. After the cell nuclei were labeled with DAPI, images were captured by Zeiss Axiophot 2 microscope. ..

Incubation:

Article Title: A Myosin I Is Involved in Membrane Recycling from Early Endosomes
Article Snippet: .. To visualize acidic compartments, adherent D . discoideum cells were incubated with LysoSensor Green DND-189 (an acidotropic probe that may localize in the membrane of acidic organelles; Molecular Probes Product information sheet) for 5 min, washed in SBS, and imaged with a Zeiss Axiophot 2 microscope using a 100× Achroplan water immersion objective. ..

Microscopy:

Article Title: Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways
Article Snippet: .. After the cell nuclei were labeled with DAPI, images were captured by Zeiss Axiophot 2 microscope. ..

Article Title: QUANTIFICATION OF SYNAPTIC DENSITY IN CORTICOSTRIATAL PROJECTIONS FROM RAT MEDIAL AGRANULAR CORTEX
Article Snippet: .. This was done using an M5 imaging system (Imaging Research, Inc., St. Catharines, Ontario, Canada) interfaced to a Zeiss Axiophot 2 microscope equipped with a motorized scanning stage and video display. .. First, the section with highest labeling density in contralateral DCS was chosen for analysis.

Article Title: A Myosin I Is Involved in Membrane Recycling from Early Endosomes
Article Snippet: .. To visualize acidic compartments, adherent D . discoideum cells were incubated with LysoSensor Green DND-189 (an acidotropic probe that may localize in the membrane of acidic organelles; Molecular Probes Product information sheet) for 5 min, washed in SBS, and imaged with a Zeiss Axiophot 2 microscope using a 100× Achroplan water immersion objective. ..

Article Title: Rapid Transgene Expression in Multiple Precursor Cell Types of Adult Rat Subventricular Zone Mediated by Adeno-Associated Type 1 Vectors
Article Snippet: .. TUNEL-positive cells were quantified with a Zeiss Axiophot 2 microscope equipped with fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) filters (Carl Zeiss, Göttingen, Germany) on five sections surrounding the injection site, covering 400 μm along the anteroposterior axis. .. Cells were counted in the SVZ ipsilateral and contralateral to the injection site.

Article Title: Mutations at Phosphorylation Sites of Xenopus Microtubule-associated Protein 4 Affect Its Microtubule-binding Ability and Chromosome Movement during Mitosis
Article Snippet: .. Photographs were taken using a Zeiss Axiophot 2 microscope ( Carl Zeiss , Thornwood, NY) with a 63× Plan-Apochromat objective lens. .. For extended time lapse observation, living cells grown in six-well plates were observed under an inverted fluorescence microscope ( Olympus IX70) with a 40× LCPlanFl objective lens.

Article Title: Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B
Article Snippet: .. Digital fluorescence microscopic images were acquired using an Axiophot 2 microscope (Zeiss, Oberkochen, Germany) equipped with a CCD camera (Visitron Systems, Puchheim, Germany) and afterwards processed with Photoshop CS3 (Adobe, San Jose, CA, USA). .. IgG fluorescence intensity in the endoneurium of femoral quadriceps nerve cross sections was measured with ImageJ (NIH, Bethesda, Maryland).

Article Title: Thyroid Hormone Signaling in the Mouse Retina
Article Snippet: .. Imaging and analysis Tissue was analyzed with a Zeiss Axiophot 2 microscope and a Zeiss Axioplan 2 equipped with epifluorescence. .. Micrographs were taken with a CCD camera and the Axiovision LE software (Carl Zeiss Vision, Germany).

Article Title: Neonatal NK cells target the mouse duct epithelium via Nkg2d and drive tissue-specific injury in experimental biliary atresia
Article Snippet: .. Images were captured using either a Zeiss Axiophot 2 microscope or an Olympus BX51 microscope with appropriate filters. ..

Injection:

Article Title: Rapid Transgene Expression in Multiple Precursor Cell Types of Adult Rat Subventricular Zone Mediated by Adeno-Associated Type 1 Vectors
Article Snippet: .. TUNEL-positive cells were quantified with a Zeiss Axiophot 2 microscope equipped with fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) filters (Carl Zeiss, Göttingen, Germany) on five sections surrounding the injection site, covering 400 μm along the anteroposterior axis. .. Cells were counted in the SVZ ipsilateral and contralateral to the injection site.

Imaging:

Article Title: QUANTIFICATION OF SYNAPTIC DENSITY IN CORTICOSTRIATAL PROJECTIONS FROM RAT MEDIAL AGRANULAR CORTEX
Article Snippet: .. This was done using an M5 imaging system (Imaging Research, Inc., St. Catharines, Ontario, Canada) interfaced to a Zeiss Axiophot 2 microscope equipped with a motorized scanning stage and video display. .. First, the section with highest labeling density in contralateral DCS was chosen for analysis.

Article Title: Thyroid Hormone Signaling in the Mouse Retina
Article Snippet: .. Imaging and analysis Tissue was analyzed with a Zeiss Axiophot 2 microscope and a Zeiss Axioplan 2 equipped with epifluorescence. .. Micrographs were taken with a CCD camera and the Axiovision LE software (Carl Zeiss Vision, Germany).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Carl Zeiss axiophot 2 microscope
    Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss <t>Axiophot</t> 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.
    Axiophot 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axiophot 2 microscope/product/Carl Zeiss
    Average 92 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    axiophot 2 microscope - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Carl Zeiss dic microscopy
    DLC-1 marks apoptotic germ cells. a DLC-1::GFP localizes to apoptotic germ cells (black arrows) in ced-6(tm1826) mutants. The apoptotic germ cells can be seen as button-like structures on the corresponding <t>DIC</t> image. b DLC-1::GFP localizes to apoptotic germ cells (black arrows) in animals treated with RNAi against ced-1 (top). Mutation of ced-3(n717) completely abolishes the accumulation of apoptotic cells due to RNAi against ced-1 (bottom). Blocking <t>apoptosis</t> removes the DLC-1::GFP rings. c Inactivation of dlc-1 in somatic tissues ( ppw-1(pk2505) mutant background) does not affect DLC-1::GFP localization to apoptotic cells. Inactivation of dlc-1 in germ cells ( rrf-1(ok589) mutant background) completely removes DLC-1::GFP localized to apoptotic cells. d DLC-1::GFP co-localizes with Mab414 antibody against the nuclear pore complex at the nuclear membrane (red) in apoptotic germ cells. DAPI (blue). Black scale bars: 10 µm
    Dic Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dic microscopy/product/Carl Zeiss
    Average 91 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    dic microscopy - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    90
    Carl Zeiss alizarin red epifluorescence
    A, C, Parr skull preparations, and B, D dissected mandibular bones along with reconstructions of how they fit together. Alizarin Red staining and photographed by <t>epifluorescence.</t> op: opercle, sop: subopercle. The preparations are shown as left side views,
    Alizarin Red Epifluorescence, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alizarin red epifluorescence/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alizarin red epifluorescence - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss Axiophot 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.

    Journal: PLoS ONE

    Article Title: Thyroid Hormone Signaling in the Mouse Retina

    doi: 10.1371/journal.pone.0168003

    Figure Lengend Snippet: Retinal wholemounts of FINDT3 mice stained with X-gal. Blue dots indicate stained cells in the GCL and INL. Left : In the adult retina, the density of stained cells is high in central retina around the optic nerve head and decreases towards the periphery. The density decline is more pronounced in the dorsal part of the retina than in the ventral part. Right : At postnatal day p10, the retina shows basically the same density gradient of stained cells as in the adult. D, dorsal; V, ventral; T, temporal; N, nasal. The scale bar applies to both retinae. Images were acquired with a Zeiss Axiophot 2 microscope; the left image is a montage of 20 frames, the right image of 9 frames.

    Article Snippet: Imaging and analysis Tissue was analyzed with a Zeiss Axiophot 2 microscope and a Zeiss Axioplan 2 equipped with epifluorescence.

    Techniques: Mouse Assay, Staining, Microscopy

    Higher power micrographs from wholemounted FINDT3 retinae stained with X-gal. For the adult mouse (left), the GCL and INL are shown separately as they are on different focal planes. For the p10 mouse (right) only the GCL is shown. Dorsal and ventral fields are from far peripheral retina. The scale bar applies to all images. Images were acquired with a Zeiss Axiophot 2 microscope.

    Journal: PLoS ONE

    Article Title: Thyroid Hormone Signaling in the Mouse Retina

    doi: 10.1371/journal.pone.0168003

    Figure Lengend Snippet: Higher power micrographs from wholemounted FINDT3 retinae stained with X-gal. For the adult mouse (left), the GCL and INL are shown separately as they are on different focal planes. For the p10 mouse (right) only the GCL is shown. Dorsal and ventral fields are from far peripheral retina. The scale bar applies to all images. Images were acquired with a Zeiss Axiophot 2 microscope.

    Article Snippet: Imaging and analysis Tissue was analyzed with a Zeiss Axiophot 2 microscope and a Zeiss Axioplan 2 equipped with epifluorescence.

    Techniques: Staining, Microscopy

    Vertical cryostat sections of FINDT3 and wildtype mouse retinae stained for β-galactosidase. Top row: X-gal staining, blue dots indicate β-gal-positive cells. In adult FINDT3 mice (left) staining occurs in the ganglion cell layer (GCL) and inner nuclear layer (INL), but not in the outer nuclear layer (ONL). In FINDT3 mice at postnatal day 10 (p10, middle) staining mainly occurs in the GCL with only few labeled cells in the INL. There is no staining in the adult wildtype control (WT, right). Bottom row: Staining with the mouse monoclonal antibody against β-gal. As in the X-gal staining, adult FINDT3 mice show two bands of stained cells in the GCL and INL, respectively. At p10 staining mainly occurs in the GCL with few labeled cells in the INL. In all these immunolabeled sections, blood vessels are also stained by the anti-mouse secondary antibody (some arrowed); in the wildtype control, the only labeled structures are blood vessels. OPL, outer plexiform layer; IPL, inner plexiform layer. Images were acquired with a Zeiss Axiophot 2 microscope (top row) and a Zeiss Axioplan 2 microscope (bottom row). The scale bar applies to all images.

    Journal: PLoS ONE

    Article Title: Thyroid Hormone Signaling in the Mouse Retina

    doi: 10.1371/journal.pone.0168003

    Figure Lengend Snippet: Vertical cryostat sections of FINDT3 and wildtype mouse retinae stained for β-galactosidase. Top row: X-gal staining, blue dots indicate β-gal-positive cells. In adult FINDT3 mice (left) staining occurs in the ganglion cell layer (GCL) and inner nuclear layer (INL), but not in the outer nuclear layer (ONL). In FINDT3 mice at postnatal day 10 (p10, middle) staining mainly occurs in the GCL with only few labeled cells in the INL. There is no staining in the adult wildtype control (WT, right). Bottom row: Staining with the mouse monoclonal antibody against β-gal. As in the X-gal staining, adult FINDT3 mice show two bands of stained cells in the GCL and INL, respectively. At p10 staining mainly occurs in the GCL with few labeled cells in the INL. In all these immunolabeled sections, blood vessels are also stained by the anti-mouse secondary antibody (some arrowed); in the wildtype control, the only labeled structures are blood vessels. OPL, outer plexiform layer; IPL, inner plexiform layer. Images were acquired with a Zeiss Axiophot 2 microscope (top row) and a Zeiss Axioplan 2 microscope (bottom row). The scale bar applies to all images.

    Article Snippet: Imaging and analysis Tissue was analyzed with a Zeiss Axiophot 2 microscope and a Zeiss Axioplan 2 equipped with epifluorescence.

    Techniques: Staining, Mouse Assay, Labeling, Immunolabeling, Microscopy

    DLC-1 marks apoptotic germ cells. a DLC-1::GFP localizes to apoptotic germ cells (black arrows) in ced-6(tm1826) mutants. The apoptotic germ cells can be seen as button-like structures on the corresponding DIC image. b DLC-1::GFP localizes to apoptotic germ cells (black arrows) in animals treated with RNAi against ced-1 (top). Mutation of ced-3(n717) completely abolishes the accumulation of apoptotic cells due to RNAi against ced-1 (bottom). Blocking apoptosis removes the DLC-1::GFP rings. c Inactivation of dlc-1 in somatic tissues ( ppw-1(pk2505) mutant background) does not affect DLC-1::GFP localization to apoptotic cells. Inactivation of dlc-1 in germ cells ( rrf-1(ok589) mutant background) completely removes DLC-1::GFP localized to apoptotic cells. d DLC-1::GFP co-localizes with Mab414 antibody against the nuclear pore complex at the nuclear membrane (red) in apoptotic germ cells. DAPI (blue). Black scale bars: 10 µm

    Journal: Cell Death & Disease

    Article Title: Dynein links engulfment and execution of apoptosis via CED-4/Apaf1 in C. elegans

    doi: 10.1038/s41419-018-1067-y

    Figure Lengend Snippet: DLC-1 marks apoptotic germ cells. a DLC-1::GFP localizes to apoptotic germ cells (black arrows) in ced-6(tm1826) mutants. The apoptotic germ cells can be seen as button-like structures on the corresponding DIC image. b DLC-1::GFP localizes to apoptotic germ cells (black arrows) in animals treated with RNAi against ced-1 (top). Mutation of ced-3(n717) completely abolishes the accumulation of apoptotic cells due to RNAi against ced-1 (bottom). Blocking apoptosis removes the DLC-1::GFP rings. c Inactivation of dlc-1 in somatic tissues ( ppw-1(pk2505) mutant background) does not affect DLC-1::GFP localization to apoptotic cells. Inactivation of dlc-1 in germ cells ( rrf-1(ok589) mutant background) completely removes DLC-1::GFP localized to apoptotic cells. d DLC-1::GFP co-localizes with Mab414 antibody against the nuclear pore complex at the nuclear membrane (red) in apoptotic germ cells. DAPI (blue). Black scale bars: 10 µm

    Article Snippet: Cells undergoing apoptosis were distinguished from normal cells as refractile, button-like discs by DIC microscopy (Zeiss Axiophot Microscope equipped with an Andor Zyla 4.2 sCMOS camera) as described .

    Techniques: Mutagenesis, Blocking Assay

    DLC-1 marks apoptotic germ cells. a DLC-1::GFP localizes to apoptotic germ cells (black arrows) in ced-6(tm1826) mutants. The apoptotic germ cells can be seen as button-like structures on the corresponding DIC image. b DLC-1::GFP localizes to apoptotic germ cells (black arrows) in animals treated with RNAi against ced-1 (top). Mutation of ced-3(n717) completely abolishes the accumulation of apoptotic cells due to RNAi against ced-1 (bottom). Blocking apoptosis removes the DLC-1::GFP rings. c Inactivation of dlc-1 in somatic tissues ( ppw-1(pk2505) mutant background) does not affect DLC-1::GFP localization to apoptotic cells. Inactivation of dlc-1 in germ cells ( rrf-1(ok589) mutant background) completely removes DLC-1::GFP localized to apoptotic cells. d DLC-1::GFP co-localizes with Mab414 antibody against the nuclear pore complex at the nuclear membrane (red) in apoptotic germ cells. DAPI (blue). Black scale bars: 10 µm

    Journal: Cell Death & Disease

    Article Title: Dynein links engulfment and execution of apoptosis via CED-4/Apaf1 in C. elegans

    doi: 10.1038/s41419-018-1067-y

    Figure Lengend Snippet: DLC-1 marks apoptotic germ cells. a DLC-1::GFP localizes to apoptotic germ cells (black arrows) in ced-6(tm1826) mutants. The apoptotic germ cells can be seen as button-like structures on the corresponding DIC image. b DLC-1::GFP localizes to apoptotic germ cells (black arrows) in animals treated with RNAi against ced-1 (top). Mutation of ced-3(n717) completely abolishes the accumulation of apoptotic cells due to RNAi against ced-1 (bottom). Blocking apoptosis removes the DLC-1::GFP rings. c Inactivation of dlc-1 in somatic tissues ( ppw-1(pk2505) mutant background) does not affect DLC-1::GFP localization to apoptotic cells. Inactivation of dlc-1 in germ cells ( rrf-1(ok589) mutant background) completely removes DLC-1::GFP localized to apoptotic cells. d DLC-1::GFP co-localizes with Mab414 antibody against the nuclear pore complex at the nuclear membrane (red) in apoptotic germ cells. DAPI (blue). Black scale bars: 10 µm

    Article Snippet: Quantification of apoptosis Cells undergoing apoptosis were distinguished from normal cells as refractile, button-like discs by DIC microscopy (Zeiss Axiophot Microscope equipped with an Andor Zyla 4.2 sCMOS camera) as described .

    Techniques: Mutagenesis, Blocking Assay

    A, C, Parr skull preparations, and B, D dissected mandibular bones along with reconstructions of how they fit together. Alizarin Red staining and photographed by epifluorescence. op: opercle, sop: subopercle. The preparations are shown as left side views,

    Journal: Evolution & development

    Article Title: Patterns of variation and covariation in the shapes of mandibular bones of juvenile salmonids in the genus Oncorhynchus

    doi: 10.1111/ede.12135

    Figure Lengend Snippet: A, C, Parr skull preparations, and B, D dissected mandibular bones along with reconstructions of how they fit together. Alizarin Red staining and photographed by epifluorescence. op: opercle, sop: subopercle. The preparations are shown as left side views,

    Article Snippet: After clearing the preparations for several days in a solution of 0.1% KOH and 50% glycerol, individual bones were carefully dissected from the skulls, mounted between bridged coverslips and photographed at low magnification (2.5× objective lens) using Alizarin Red epifluorescence (Zeiss Axiophot microscope).

    Techniques: Staining