Structured Review

Carl Zeiss axioimager m2 microscope
Axioimager M2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axioimager m2 microscope/product/Carl Zeiss
Average 92 stars, based on 81 article reviews
Price from $9.99 to $1999.99
axioimager m2 microscope - by Bioz Stars, 2021-01
92/100 stars

Images

Related Articles

Fluorescence:

Article Title: Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells
Article Snippet: .. Fluorescence images were taken with an AxioImager M2 microscope, equipped with a 100× oil immersion objective (Carl Zeiss Microscope, Jena, Germany) at a resolution of 2048 × 2048 pixels. ..

Microscopy:

Article Title: The NTP generating activity of pyruvate kinase II is critical for apicoplast maintenance in Plasmodium falciparum
Article Snippet: .. Cells were resuspended in 20 μL of CMA and then pipetted onto slides and sealed with wax for observation on a Zeiss AxioImager M2 microscope. .. A series of images spanning 5 µm were acquired with 0.2 µm spacing and images were deconvolved with VOLOCITY software (PerkinElmer) to report a single image in the z-plane.

Article Title: Plasmodium berghei Kinesin-5 Associates With the Spindle Apparatus During Cell Division and Is Important for Efficient Production of Infectious Sporozoites
Article Snippet: .. These fixed gametocytes were then examined on a Zeiss AxioImager M2 microscope. .. Fixed Immunofluorescence AssayThe purified schizonts from PbKinesin-5-GFP parasites were fixed in 2% paraformaldehyde (PFA) and smeared on poly-L-lysine coated slides.

Article Title: The C-Fern (Ceratopteris richardii) genome: insights into plant genome evolution with the first partial homosporous fern genome assembly
Article Snippet: .. The BAC FISH images were taken on an AxioImager M2 microscope with an AxioCam MR camera (Carl Zeiss AG, Oberkochen, Germany). ..

Article Title: Melatonin treatment of repetitive behavioral deficits in the Cntnap2 mouse model of autism spectrum disorder
Article Snippet: .. Stereological analysis was performed using a Zeiss AxioImager M2 microscope (Zeiss, Pleasanton, CA) equipped with a motorized stage controlled by the StereoInvestigator software (MicroBrightField Biosciences, Williston, VT). ..

Article Title: The genetic paradigms of dietary restriction fail to extend life span in cep-1(gk138) mutant of C. elegans p53 due to possible background mutations
Article Snippet: .. When they reach the L3 larval stage, worms were anesthetised with 20 mM sodium azide on 2% agarose slides and imaged at 630X using an AxioImager M2 microscope fitted with Axiocam MRc camera (Carl Zeiss, Germany). ..

Article Title: Time- and Dose-Dependent Effects of Ionizing Irradiation on the Membrane Expression of Hsp70 on Glioma Cells
Article Snippet: .. Fluorescence images were taken with an AxioImager M2 microscope, equipped with a 100× oil immersion objective (Carl Zeiss Microscope, Jena, Germany) at a resolution of 2048 × 2048 pixels. ..

Article Title: Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation
Article Snippet: .. Localisation of NDC80–GFP throughout the parasite life cycleLive-cell imaging of transgenic parasite lines was performed at different proliferative stages during the parasite life cycle , as described previously ( ; ), using a Zeiss AxioImager M2 microscope fitted with an AxioCam ICc1 digital camera (Carl Zeiss, Inc) and 10×, 63× and 100× objectives. .. Blood stage schizogony Infected mouse blood provided asexual blood and gametocyte stages of the P. berghei life cycle.

Article Title: Plasmodium DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission
Article Snippet: .. Images were collected with an AxioCam ICc1 digital camera fitted to a Zeiss AxioImager M2 microscope using a 63x oil immersion objective. .. Statistical analyses were performed using GraphPad prism software.

Transgenic Assay:

Article Title: Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation
Article Snippet: .. Localisation of NDC80–GFP throughout the parasite life cycleLive-cell imaging of transgenic parasite lines was performed at different proliferative stages during the parasite life cycle , as described previously ( ; ), using a Zeiss AxioImager M2 microscope fitted with an AxioCam ICc1 digital camera (Carl Zeiss, Inc) and 10×, 63× and 100× objectives. .. Blood stage schizogony Infected mouse blood provided asexual blood and gametocyte stages of the P. berghei life cycle.

Imaging:

Article Title: Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation
Article Snippet: .. Localisation of NDC80–GFP throughout the parasite life cycleLive-cell imaging of transgenic parasite lines was performed at different proliferative stages during the parasite life cycle , as described previously ( ; ), using a Zeiss AxioImager M2 microscope fitted with an AxioCam ICc1 digital camera (Carl Zeiss, Inc) and 10×, 63× and 100× objectives. .. Blood stage schizogony Infected mouse blood provided asexual blood and gametocyte stages of the P. berghei life cycle.

BAC Assay:

Article Title: The C-Fern (Ceratopteris richardii) genome: insights into plant genome evolution with the first partial homosporous fern genome assembly
Article Snippet: .. The BAC FISH images were taken on an AxioImager M2 microscope with an AxioCam MR camera (Carl Zeiss AG, Oberkochen, Germany). ..

Fluorescence In Situ Hybridization:

Article Title: The C-Fern (Ceratopteris richardii) genome: insights into plant genome evolution with the first partial homosporous fern genome assembly
Article Snippet: .. The BAC FISH images were taken on an AxioImager M2 microscope with an AxioCam MR camera (Carl Zeiss AG, Oberkochen, Germany). ..

Software:

Article Title: Melatonin treatment of repetitive behavioral deficits in the Cntnap2 mouse model of autism spectrum disorder
Article Snippet: .. Stereological analysis was performed using a Zeiss AxioImager M2 microscope (Zeiss, Pleasanton, CA) equipped with a motorized stage controlled by the StereoInvestigator software (MicroBrightField Biosciences, Williston, VT). ..

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  • 92
    Carl Zeiss lsm image browser software
    Effect of parthenolide on tubercles of S. mansoni male worms. The quantification of the number of tubercles was performed using confocal microscopy. Indicated are numbers of intact tubercles and these numbers were measured in a 20,000 μ m 2 of area calculated with the <t>Zeiss</t> <t>LSM</t> Image Browser software. Praziquantel (PZQ, 5 μ M) was used as positive control. A minimum of three tegument areas of each parasite were assessed. Values are means ± SD (bars) of ten male adult worms. ∗∗∗ P
    Lsm Image Browser Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lsm image browser software/product/Carl Zeiss
    Average 92 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    lsm image browser software - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    90
    Carl Zeiss imager m2 microscope
    CPZ alters the cell surface localization of PrP C . A. Cells were seeded on glass coverslips and grown for 24 h to ~60% confluence. For surface staining of PrP, cells were first incubated at 4°C with antibody D18 diluted, then fixed with paraformaldehyde and incubated with fluorescently-labelled secondary antibody. For total PrP staining, cells were permeabilized with Triton X-100, fixed with paraformaldehyde, and then incubated with primary and secondary antibodies. Coverslips were mounted with Fluor-save Reagent (Calbiochem), and analyzed with a Zeiss Imager <t>M2</t> microscope. B. N2a cells stably expressing mouse WT PrP C were grown to confluence on glass coverslips, and treated with the indicated concentrations of Fe(III)-TMPyP or CPZ for 24h. For detection of surface PrP C (SC#1, shown in the picture), coverslips were incubated in ice with antibody 6D11 (this step was omitted for detection of total PrP C , not shown). Coverslips were blotted on a nitrocellulose membrane soaked in lysis buffer, and incubated with horseradish peroxidase-conjugated secondary antibody. For detection of total PrP C , cell blots were incubated with the primary and secondary antibodies. The PrP C signal was revealed by enhanced chemiluminescence. C. PrP C signal was quantitated by densitometry. The bar graph shows the % ratio of surface to total PrP C . Each bar represents the mean (± standard error) of three independent experiments (n = 3). Statistically-significant differences (*), estimated by Student t -test, between CPZ-treated and untreated cells were as follow: [3 μM], p = 0.0058; [10 μM], p = 0.00034.
    Imager M2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imager m2 microscope/product/Carl Zeiss
    Average 90 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    imager m2 microscope - by Bioz Stars, 2021-01
    90/100 stars
      Buy from Supplier

    92
    Carl Zeiss axio imager m2 microscope
    ( a ) No significant difference in the number of leukocytes was detected in whole blood. ( b ) Amount of band and segmented neutrophils in stained blood smears. ( c ) Representative images show the morphology of neutrophils in fresh (0 h) and stored blood (24 h) after HAEMA fast stain (band = arrow, segmented = arrowhead). The images were captured with a Zeiss <t>Axio</t> Imager M2 microscope and taken with a 63× objective. Data in a and b were analyzed with one-tailed paired Student’s t -test ( n = 9) and presented with mean ± SD (**** p
    Axio Imager M2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axio imager m2 microscope/product/Carl Zeiss
    Average 92 stars, based on 636 article reviews
    Price from $9.99 to $1999.99
    axio imager m2 microscope - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effect of parthenolide on tubercles of S. mansoni male worms. The quantification of the number of tubercles was performed using confocal microscopy. Indicated are numbers of intact tubercles and these numbers were measured in a 20,000 μ m 2 of area calculated with the Zeiss LSM Image Browser software. Praziquantel (PZQ, 5 μ M) was used as positive control. A minimum of three tegument areas of each parasite were assessed. Values are means ± SD (bars) of ten male adult worms. ∗∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Flavonoids and Sesquiterpene Lactones from Artemisia absinthium and Tanacetum parthenium against Schistosoma mansoni Worms

    doi: 10.1155/2016/9521349

    Figure Lengend Snippet: Effect of parthenolide on tubercles of S. mansoni male worms. The quantification of the number of tubercles was performed using confocal microscopy. Indicated are numbers of intact tubercles and these numbers were measured in a 20,000 μ m 2 of area calculated with the Zeiss LSM Image Browser software. Praziquantel (PZQ, 5 μ M) was used as positive control. A minimum of three tegument areas of each parasite were assessed. Values are means ± SD (bars) of ten male adult worms. ∗∗∗ P

    Article Snippet: The numbers of tubercles was counted in 20,000 μ m2 of area calculated with the Zeiss LSM Image Browser software.

    Techniques: Confocal Microscopy, Software, Positive Control

    CPZ alters the cell surface localization of PrP C . A. Cells were seeded on glass coverslips and grown for 24 h to ~60% confluence. For surface staining of PrP, cells were first incubated at 4°C with antibody D18 diluted, then fixed with paraformaldehyde and incubated with fluorescently-labelled secondary antibody. For total PrP staining, cells were permeabilized with Triton X-100, fixed with paraformaldehyde, and then incubated with primary and secondary antibodies. Coverslips were mounted with Fluor-save Reagent (Calbiochem), and analyzed with a Zeiss Imager M2 microscope. B. N2a cells stably expressing mouse WT PrP C were grown to confluence on glass coverslips, and treated with the indicated concentrations of Fe(III)-TMPyP or CPZ for 24h. For detection of surface PrP C (SC#1, shown in the picture), coverslips were incubated in ice with antibody 6D11 (this step was omitted for detection of total PrP C , not shown). Coverslips were blotted on a nitrocellulose membrane soaked in lysis buffer, and incubated with horseradish peroxidase-conjugated secondary antibody. For detection of total PrP C , cell blots were incubated with the primary and secondary antibodies. The PrP C signal was revealed by enhanced chemiluminescence. C. PrP C signal was quantitated by densitometry. The bar graph shows the % ratio of surface to total PrP C . Each bar represents the mean (± standard error) of three independent experiments (n = 3). Statistically-significant differences (*), estimated by Student t -test, between CPZ-treated and untreated cells were as follow: [3 μM], p = 0.0058; [10 μM], p = 0.00034.

    Journal: PLoS ONE

    Article Title: An antipsychotic drug exerts anti-prion effects by altering the localization of the cellular prion protein

    doi: 10.1371/journal.pone.0182589

    Figure Lengend Snippet: CPZ alters the cell surface localization of PrP C . A. Cells were seeded on glass coverslips and grown for 24 h to ~60% confluence. For surface staining of PrP, cells were first incubated at 4°C with antibody D18 diluted, then fixed with paraformaldehyde and incubated with fluorescently-labelled secondary antibody. For total PrP staining, cells were permeabilized with Triton X-100, fixed with paraformaldehyde, and then incubated with primary and secondary antibodies. Coverslips were mounted with Fluor-save Reagent (Calbiochem), and analyzed with a Zeiss Imager M2 microscope. B. N2a cells stably expressing mouse WT PrP C were grown to confluence on glass coverslips, and treated with the indicated concentrations of Fe(III)-TMPyP or CPZ for 24h. For detection of surface PrP C (SC#1, shown in the picture), coverslips were incubated in ice with antibody 6D11 (this step was omitted for detection of total PrP C , not shown). Coverslips were blotted on a nitrocellulose membrane soaked in lysis buffer, and incubated with horseradish peroxidase-conjugated secondary antibody. For detection of total PrP C , cell blots were incubated with the primary and secondary antibodies. The PrP C signal was revealed by enhanced chemiluminescence. C. PrP C signal was quantitated by densitometry. The bar graph shows the % ratio of surface to total PrP C . Each bar represents the mean (± standard error) of three independent experiments (n = 3). Statistically-significant differences (*), estimated by Student t -test, between CPZ-treated and untreated cells were as follow: [3 μM], p = 0.0058; [10 μM], p = 0.00034.

    Article Snippet: Coverslips were mounted with Fluor-save Reagent (Calbiochem), and analyzed with a Zeiss Imager M2 microscope.

    Techniques: Staining, Incubation, Microscopy, Stable Transfection, Expressing, Lysis

    ( a ) No significant difference in the number of leukocytes was detected in whole blood. ( b ) Amount of band and segmented neutrophils in stained blood smears. ( c ) Representative images show the morphology of neutrophils in fresh (0 h) and stored blood (24 h) after HAEMA fast stain (band = arrow, segmented = arrowhead). The images were captured with a Zeiss Axio Imager M2 microscope and taken with a 63× objective. Data in a and b were analyzed with one-tailed paired Student’s t -test ( n = 9) and presented with mean ± SD (**** p

    Journal: Biomedicines

    Article Title: How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs

    doi: 10.3390/biomedicines8080278

    Figure Lengend Snippet: ( a ) No significant difference in the number of leukocytes was detected in whole blood. ( b ) Amount of band and segmented neutrophils in stained blood smears. ( c ) Representative images show the morphology of neutrophils in fresh (0 h) and stored blood (24 h) after HAEMA fast stain (band = arrow, segmented = arrowhead). The images were captured with a Zeiss Axio Imager M2 microscope and taken with a 63× objective. Data in a and b were analyzed with one-tailed paired Student’s t -test ( n = 9) and presented with mean ± SD (**** p

    Article Snippet: Example pictures were made with a Zeiss Axio Imager M2 microscope with a Plan-Apochromat 63×/1.4 Oil DIC ∞/0.17 objective.

    Techniques: Staining, Microscopy, One-tailed Test

    Selected bacterial counts (log cfu/ml digesta) in digesta determined by DAPI staining (total number of bacteria) and fluorescent in situ hybridization (FISH). Hybridization was performed with oligonucleotides, whose sequence was based on the literature. A Carl Zeiss Microscope Axio Imager M2 (Carl Zeiss Jena GmbH, Jena, Germany) was used to determine bacterial count.

    Journal: PLoS ONE

    Article Title: Synbiotics for Broiler Chickens—In Vitro Design and Evaluation of the Influence on Host and Selected Microbiota Populations following In Ovo Delivery

    doi: 10.1371/journal.pone.0168587

    Figure Lengend Snippet: Selected bacterial counts (log cfu/ml digesta) in digesta determined by DAPI staining (total number of bacteria) and fluorescent in situ hybridization (FISH). Hybridization was performed with oligonucleotides, whose sequence was based on the literature. A Carl Zeiss Microscope Axio Imager M2 (Carl Zeiss Jena GmbH, Jena, Germany) was used to determine bacterial count.

    Article Snippet: To distinguish bacteria from other particles in the ileal samples (DAPI), filters were left at 4°C for 1 h in the dark until visualization with a Carl Zeiss Microscope Axio Imager M2 (Carl Zeiss Jena GmbH, Jena, Germany), as described elsewhere [ , ].

    Techniques: Staining, In Situ Hybridization, Fluorescence In Situ Hybridization, Hybridization, Sequencing, Microscopy