axioimager a1 upright epifluorescent microscope  (Carl Zeiss)

 
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    Structured Review

    Carl Zeiss axioimager a1 upright epifluorescent microscope
    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss <t>AxioImager.A1</t> upright <t>epifluorescent</t> microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p
    Axioimager A1 Upright Epifluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioimager a1 upright epifluorescent microscope/product/Carl Zeiss
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    axioimager a1 upright epifluorescent microscope - by Bioz Stars, 2020-11
    88/100 stars

    Images

    1) Product Images from "Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal"

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0081387

    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p
    Figure Legend Snippet: The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Techniques Used: Stable Transfection, Expressing, Incubation, Microscopy, Software, Staining

    2) Product Images from "Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal"

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0081387

    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p
    Figure Legend Snippet: The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Techniques Used: Stable Transfection, Expressing, Incubation, Microscopy, Software, Staining

    Related Articles

    Microscopy:

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with Axio Vision LE 4.6 image acquisition software. .. Supporting Information FANCD2 contains a highly conserved amino-terminal nuclear localization signal, which facilitates nuclear expression of GFP. ( A ) cNLS mapper ( http://nls-mapper.iab.keio.ac.jp/cgi-bin/NLS_Mapper_form.cgi ) was used to analyze the FANCD2 amino acid sequence for importin α/β-dependent nuclear localization signals (NLSs), and identified amino acids 2-58 as harboring several putative high scoring bipartite NLSs ( B ). ( C ) A Clustal Omega ( http://www.ebi.ac.uk/Tools/msa/clustalo/ ) multiple sequence alignment of full length FANCD2 corresponding to Figure 1A .

    Article Title: Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor
    Article Snippet: .. Metaphase chromosomes were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. ..

    Article Title: Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein
    Article Snippet: .. Metaphase chromosomes were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. ..

    Article Title: p21 promotes error-free replication-coupled DNA double-strand break repair
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. siRNA experiments HeLa cells were plated in six-well dishes at a density of 200 000 cells per well and transfected the following day with p21-specific (sip21) or control, non-targeting siRNA (siControl [siCtrl]) using Lipofectamine 2000 (Invitrogen).

    Article Title: p21 promotes error-free replication-coupled DNA double-strand break repair
    Article Snippet: .. Cells were then counterstained and mounted in vectashield plus 4′6-diamidine-2-phenylindole dihydrochloride (Vector Laboratories) and visualized using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-53BP1 (H300; Santa Cruz Biotechnology), anti-BRCA1 (D9; Santa Cruz Biotechnology), anti-CtIP (a kind gift from Richard Baer, Columbia University) and anti-MRE11 (PC388; Calbiochem/EMD Chemicals).

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal
    Article Snippet: .. Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-FANCD2 (NB100-182; Novus Biologicals), anti-FANCI (A300-212A; Bethyl Laboratories), anti-DNA-PKCS pS2056 (ab18192; Abcam), and anti-V5 (R960-25; Invitrogen).

    Article Title: Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein
    Article Snippet: .. Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Cells were lysed in NETN100 (20mM Tris-HCl pH 7.4, 0.1% v/v NP-40, 100mM NaCl, 1mM EDTA, 1mM Na3 O4 V, 1mM NaF, supplemented with protease inhibitors), incubated on ice and sonicated briefly.

    Software:

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with Axio Vision LE 4.6 image acquisition software. .. Supporting Information FANCD2 contains a highly conserved amino-terminal nuclear localization signal, which facilitates nuclear expression of GFP. ( A ) cNLS mapper ( http://nls-mapper.iab.keio.ac.jp/cgi-bin/NLS_Mapper_form.cgi ) was used to analyze the FANCD2 amino acid sequence for importin α/β-dependent nuclear localization signals (NLSs), and identified amino acids 2-58 as harboring several putative high scoring bipartite NLSs ( B ). ( C ) A Clustal Omega ( http://www.ebi.ac.uk/Tools/msa/clustalo/ ) multiple sequence alignment of full length FANCD2 corresponding to Figure 1A .

    Article Title: Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor
    Article Snippet: .. Metaphase chromosomes were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. ..

    Article Title: Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein
    Article Snippet: .. Metaphase chromosomes were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. ..

    Article Title: p21 promotes error-free replication-coupled DNA double-strand break repair
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. siRNA experiments HeLa cells were plated in six-well dishes at a density of 200 000 cells per well and transfected the following day with p21-specific (sip21) or control, non-targeting siRNA (siControl [siCtrl]) using Lipofectamine 2000 (Invitrogen).

    Article Title: p21 promotes error-free replication-coupled DNA double-strand break repair
    Article Snippet: .. Cells were then counterstained and mounted in vectashield plus 4′6-diamidine-2-phenylindole dihydrochloride (Vector Laboratories) and visualized using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-53BP1 (H300; Santa Cruz Biotechnology), anti-BRCA1 (D9; Santa Cruz Biotechnology), anti-CtIP (a kind gift from Richard Baer, Columbia University) and anti-MRE11 (PC388; Calbiochem/EMD Chemicals).

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal
    Article Snippet: .. Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-FANCD2 (NB100-182; Novus Biologicals), anti-FANCI (A300-212A; Bethyl Laboratories), anti-DNA-PKCS pS2056 (ab18192; Abcam), and anti-V5 (R960-25; Invitrogen).

    Article Title: Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein
    Article Snippet: .. Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. .. Cells were lysed in NETN100 (20mM Tris-HCl pH 7.4, 0.1% v/v NP-40, 100mM NaCl, 1mM EDTA, 1mM Na3 O4 V, 1mM NaF, supplemented with protease inhibitors), incubated on ice and sonicated briefly.

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  • 88
    Carl Zeiss axioimager a1 upright epifluorescent microscope
    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss <t>AxioImager.A1</t> upright <t>epifluorescent</t> microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p
    Axioimager A1 Upright Epifluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioimager a1 upright epifluorescent microscope/product/Carl Zeiss
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    axioimager a1 upright epifluorescent microscope - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

    Image Search Results


    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Journal: PLoS ONE

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal

    doi: 10.1371/journal.pone.0081387

    Figure Lengend Snippet: The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Article Snippet: Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software.

    Techniques: Stable Transfection, Expressing, Incubation, Microscopy, Software, Staining