axioimager a1 upright epifluorescence microscope  (Carl Zeiss)

 
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    Structured Review

    Carl Zeiss axioimager a1 upright epifluorescence microscope
    Axioimager A1 Upright Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioimager a1 upright epifluorescence microscope/product/Carl Zeiss
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    axioimager a1 upright epifluorescence microscope - by Bioz Stars, 2020-11
    90/100 stars

    Related Products / Commonly Used Together

    406-diamidine-2-phenylindole dihydrochloride dapi
    vectashield

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    Microscopy:

    Article Title: FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE (v4.6) image acquisition software. .. Glutathione-agarose (Invitrogen) was blocked in NETN150 (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25% [vol/vol] NP-40, 1 mM EDTA) plus 1% (wt/vol) bovine serum albumin, washed in NETN150, and added to GST fusion proteins overnight at 4°C.

    Article Title: FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair
    Article Snippet: .. Foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE (v4.6) image acquisition software. .. Proximity ligation assays (PLA) were performed similarly to the immunofluorescence microscopy protocol up to the secondary antibody step.

    Article Title: The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair
    Article Snippet: .. Cells were then counterstained and mounted in vectashield plus 406-diamidine-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories) and visualized using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-53BP1 (H300; Santa Cruz Biotechnology), anti-DNA-PKcs pS2056 (ab18192; Abcam), anti-FANCA (ABP6201; Cascade), anti-FANCD2 (NB100–182; Novus Biologicals and sc-20022; Santa Cruz Biotechnology), anti-FANCI (A300-212A; Bethyl Laboratories), anti-FANCM (Meetei and Deans CE56.1 antibodies), anti-FK2 (sc-8017; Santa Cruz Biotechnology), and anti-γH2AX (05–636; Millipore).

    Article Title: Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure
    Article Snippet: .. Nuclear foci were scored using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-FANCD2 (NB100-182; Novus Biologicals), anti-FANCI (A300-212A; Bethyl Laboratories), and anti-γH2AX (05-636; Millipore).

    Software:

    Article Title: FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair
    Article Snippet: .. Metaphases were analyzed using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE (v4.6) image acquisition software. .. Glutathione-agarose (Invitrogen) was blocked in NETN150 (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25% [vol/vol] NP-40, 1 mM EDTA) plus 1% (wt/vol) bovine serum albumin, washed in NETN150, and added to GST fusion proteins overnight at 4°C.

    Article Title: FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair
    Article Snippet: .. Foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE (v4.6) image acquisition software. .. Proximity ligation assays (PLA) were performed similarly to the immunofluorescence microscopy protocol up to the secondary antibody step.

    Article Title: The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair
    Article Snippet: .. Cells were then counterstained and mounted in vectashield plus 406-diamidine-2-phenylindole dihydrochloride (DAPI) (Vector Laboratories) and visualized using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-53BP1 (H300; Santa Cruz Biotechnology), anti-DNA-PKcs pS2056 (ab18192; Abcam), anti-FANCA (ABP6201; Cascade), anti-FANCD2 (NB100–182; Novus Biologicals and sc-20022; Santa Cruz Biotechnology), anti-FANCI (A300-212A; Bethyl Laboratories), anti-FANCM (Meetei and Deans CE56.1 antibodies), anti-FK2 (sc-8017; Santa Cruz Biotechnology), and anti-γH2AX (05–636; Millipore).

    Article Title: Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure
    Article Snippet: .. Nuclear foci were scored using a Zeiss AxioImager.A1 upright epifluorescence microscope with AxioVision LE 4.6 image acquisition software. .. Primary antibodies used for IF were anti-FANCD2 (NB100-182; Novus Biologicals), anti-FANCI (A300-212A; Bethyl Laboratories), and anti-γH2AX (05-636; Millipore).

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  • 88
    Carl Zeiss axioimager a1 upright epifluorescent microscope
    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss <t>AxioImager.A1</t> upright <t>epifluorescent</t> microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p
    Axioimager A1 Upright Epifluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axioimager a1 upright epifluorescent microscope/product/Carl Zeiss
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    axioimager a1 upright epifluorescent microscope - by Bioz Stars, 2020-11
    88/100 stars
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    The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Journal: PLoS ONE

    Article Title: Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal

    doi: 10.1371/journal.pone.0081387

    Figure Lengend Snippet: The FANCD2 NLS mutants fail to correct the ICL sensitivity of FA-D2 patient cells. ( A ) FA-D2 cells stably expressing LacZ, FANCD2-WT, FANCD2-∆N57, FANCD2-∆N100, FANCD2-3N, or FANCD2-K561R were incubated in the absence or presence of 8 or 16 nM MMC for 24 h and the numbers of chromosome aberrations including gaps and breaks, dicentrics, and complex chromosome aberrations, including radial formations, were scored. Metaphase spreads were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software. At least 80 metaphases were scored per treatment. Error bars represent the standard error of the means. ( B ) FA-D2 cells stably expressing LacZ, FANCD2-WT and FANCD2-∆N57 were incubated in the absence (NT) or presence of 40 nM MMC for 18 h, and allowed to recover for 0, 4.5 or 7 h. Cells were then fixed, stained with rabbit polyclonal anti-DNA-PK CS pS2056, and counterstained with DAPI. At least 300 cells were scored for nuclei with > 5 DNA-PK CS foci. Error bars represent the standard error of the means from two independent experiments. ***, p

    Article Snippet: Nuclear foci were analyzed using a Zeiss AxioImager.A1 upright epifluorescent microscope with AxioVision LE 4.6 image acquisition software.

    Techniques: Stable Transfection, Expressing, Incubation, Microscopy, Software, Staining