avidin d texas red  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Avidin D
    Description:
    Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous ultrapure avidin with very low nonspecific binding characteristics Avidin D is recommended for use in solid phase assays and many histochemical applications when the technique of sequential addition of reagents sandwich technique is employed
    Catalog Number:
    a-2000
    Price:
    None
    Size:
    10 mg
    Category:
    Proteins
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin d texas red
    Avidin D
    Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous ultrapure avidin with very low nonspecific binding characteristics Avidin D is recommended for use in solid phase assays and many histochemical applications when the technique of sequential addition of reagents sandwich technique is employed
    https://www.bioz.com/result/avidin d texas red/product/Vector Laboratories
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    avidin d texas red - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Adult onset cardiac dilatation in a transgenic mouse line with Gal?1,3GalNAc ?2,3-sialyltransferase II (ST3Gal-II) transgenes: a new model for dilated cardiomyopathy
    Article Snippet: .. The lectins were visualized by 0.1 mg/mL FITC-avidin D (Vector Laboratories) under a fluorescence microscope (OPTIPHOTO, Nikon Corp., Tokyo, Japan) with a digital image capture unit (DXC-S500/OL, Olympus). ..

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Avidin-Biotin Assay:

    Article Title: Comparative analysis of involvement of UGT1 and UGT2 splice variants of UDP-galactose transporter in glycosylation of macromolecules in MDCK and CHO cell lines
    Article Snippet: .. Lectins bound to specific glycans were subsequently detected using alkaline phosphatase-conjugated avidin D (Vector Laboratories) and visualized with NBT/BCIP solution (Roche) according to the manufacturer’s instructions. .. Isolation of N-glycans from MDCK and CHO cells Cells (∼107 ) grown in respective serum-free media were scraped from tissue culture plates, washed twice with 15 ml of cold PBS and collected at 200×g for 5 min. Then, cells were lysed on ice in 500 μl of lysis buffer (20 mM Tris–HCl pH 7.4, 150 mM NaCl), containing 1% Nonidet NP-40 and Complete protease inhibitors (without EDTA) for 1 h. After incubation in lysis buffer, samples were additionally sonicated 3 times, each time with 30 s pulses and 2-min cooling between pulses.

    Article Title: Expression of ?F508 Cystic Fibrosis Transmembrane Regulator (CFTR) Decreases Membrane Sialylation
    Article Snippet: .. Reagents Fluorescein and Biotin conjugates of Maackia amaurensis lectin (MAL II), avidin D-alkaline phosphatase, Vector Laboratories (Burlingame, CA); biotinlylated conjugate of cholera toxin, List Biological Lab, Campbell, CA. .. ; N-acetylneuraminic acid and N-glycolylneuraminic acid1,2 diamino-4,5-methylenedioxybenzene, N-propyl gallate, N-ethylmaleimide, PMSF, leupeptin, TBST, goat anti-rabbit alkaline phosphatase, Sigma Chemical Company, St. Louis, MO; Biorad protein standard assay, alkaline phosphatase substrate kit (p-nitrophenylphosphate, diethanolamine buffer), BioRad, Hercules, CA.

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Article Title: miR-34 activity is modulated through 5′-end phosphorylation in response to DNA damage
    Article Snippet: .. Reactions were incubated for 15 min at room temperature and 25 μl of Avidin-D Agarose (Vector Labs) was added. .. Avidin-D Agarose was washed with Buffer B+500 mM NaCl and RNA was extracted with phenol.

    Article Title: Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes
    Article Snippet: .. In some cases, IgM was cross-linked using polyclonal goat anti-bovine IgM or monoclonal mouse anti-bovine IgM (BM-23; Sigma) coupled to agarose avidin D beads (Vector Laboratories, Burlingame, CA) at a concentration of 50–60 µl of 1 mg/ml antibody-coated beads per ml of culture. .. Culture of B cells with a murine fibroblast cell line, DAP3, stably transfected with bovine CD40L (boCD40L–DAP3) was carried out at a ratio of one CD40L–DAP3 cell to every four B cells.

    Microscopy:

    Article Title: Adult onset cardiac dilatation in a transgenic mouse line with Gal?1,3GalNAc ?2,3-sialyltransferase II (ST3Gal-II) transgenes: a new model for dilated cardiomyopathy
    Article Snippet: .. The lectins were visualized by 0.1 mg/mL FITC-avidin D (Vector Laboratories) under a fluorescence microscope (OPTIPHOTO, Nikon Corp., Tokyo, Japan) with a digital image capture unit (DXC-S500/OL, Olympus). ..

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Concentration Assay:

    Article Title: Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes
    Article Snippet: .. In some cases, IgM was cross-linked using polyclonal goat anti-bovine IgM or monoclonal mouse anti-bovine IgM (BM-23; Sigma) coupled to agarose avidin D beads (Vector Laboratories, Burlingame, CA) at a concentration of 50–60 µl of 1 mg/ml antibody-coated beads per ml of culture. .. Culture of B cells with a murine fibroblast cell line, DAP3, stably transfected with bovine CD40L (boCD40L–DAP3) was carried out at a ratio of one CD40L–DAP3 cell to every four B cells.

    Incubation:

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Article Title: miR-34 activity is modulated through 5′-end phosphorylation in response to DNA damage
    Article Snippet: .. Reactions were incubated for 15 min at room temperature and 25 μl of Avidin-D Agarose (Vector Labs) was added. .. Avidin-D Agarose was washed with Buffer B+500 mM NaCl and RNA was extracted with phenol.

    Blocking Assay:

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Immunostaining:

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Binding Assay:

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Software:

    Article Title: Changes in the Expression of Genes Associated with Intraneuronal Amyloid Beta and Tau in Alzheimer's Disease
    Article Snippet: .. After incubation with antibodies to either Aβ or P-tau, antibody binding was detected with biotinylated anti-rabbit or mouse IgG (1/200 dilution; 20 min at room temperature; Vector Laboratories, Burlingame, CA), a 1:300 dilution of FITC-avidin DCS (Vector Laboratories) for 5 min, followed by avidin/biotin blocking for 15 min, and then immunostaining with BDNF, TrkB, or DYN and detection with biotinylated anti-rabbit or mouse IgG and Texas Red DCS for 5 min. Psuedocolor images were captured using a Nikon fluorescence microscope with FITC and Texas Red filters using IP Lab software (Fairfax, VA) and merged using Adobe Photoshop (San Jose, CA). .. The number of Aβ, P-tau, BDNF, TrkB, and DYN positive cells from 3–16 high-power fields (x200) were chosen from the pyramidal layer in areas with the highest density of immunostaining for three AD (AD5, AD6, AD7) and three controls (C5, C6, C7).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Vector Laboratories texas red avidin d
    Lack of Shiga-like toxin (SLT)-binding sites in germinal centres of the lymph nodes and Peyer’s patches from ICR mice. Acetone-fixed frozen sections of normal mouse kidneys [(a), (b) and (g)], mouse draining lymph nodes 6 days after subcutaneous immunization [(c), (d) and (e)], or mouse Peyer’s patches 4 days after oral immunization [(f) and (h),], were stained with digoxigenin-labelled SLT-1B proteins (DIG-SLT-1B) plus horseradish peroxidase (HRP)-anti-DIG [(a) and (b)]. The sections were then counterstained with haematoxylin. The other sections were stained with haematoxylin and eosin (c); biotin-conjugated peanut agglutinin (PNA) plus Texas Red <t>avidin</t> D (d) and (f); or DIG-SLT-1B plus fluorescein isothiocyanate (FITC)-anti-DIG (e), (g) and (h).(a): Cortical regions of the kidney. Renal tubules were strongly stained with DIG-SLT-1B (arrowheads) while glomeruli were not stained (single arrow).(b): Medullary regions of the kidney. Cross-sections of collecting ducts were strongly stained with DIG-SLT-1B.(d), (e), (f) and (h): Germinal centres (arrows) were strongly stained with PNA but not with DIG-SLT-1B. Arrowheads point towards the intestinal epithelium (f) and (h).(g): Collecting ducts in kidney medulla were strongly stained with DIG-SLT-1B, as revealed by immunofluorescence. Bars represent 50 µm [(a) and (b), × 230 magnification] or 200 µm [(c) to (h), × 70 magnification].
    Texas Red Avidin D, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red avidin d/product/Vector Laboratories
    Average 97 stars, based on 156 article reviews
    Price from $9.99 to $1999.99
    texas red avidin d - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    96
    Vector Laboratories fluorescent marker
    Lack of Shiga-like toxin (SLT)-binding sites in germinal centres of the lymph nodes and Peyer’s patches from ICR mice. Acetone-fixed frozen sections of normal mouse kidneys [(a), (b) and (g)], mouse draining lymph nodes 6 days after subcutaneous immunization [(c), (d) and (e)], or mouse Peyer’s patches 4 days after oral immunization [(f) and (h),], were stained with digoxigenin-labelled SLT-1B proteins (DIG-SLT-1B) plus horseradish peroxidase (HRP)-anti-DIG [(a) and (b)]. The sections were then counterstained with haematoxylin. The other sections were stained with haematoxylin and eosin (c); biotin-conjugated peanut agglutinin (PNA) plus Texas Red <t>avidin</t> D (d) and (f); or DIG-SLT-1B plus fluorescein isothiocyanate (FITC)-anti-DIG (e), (g) and (h).(a): Cortical regions of the kidney. Renal tubules were strongly stained with DIG-SLT-1B (arrowheads) while glomeruli were not stained (single arrow).(b): Medullary regions of the kidney. Cross-sections of collecting ducts were strongly stained with DIG-SLT-1B.(d), (e), (f) and (h): Germinal centres (arrows) were strongly stained with PNA but not with DIG-SLT-1B. Arrowheads point towards the intestinal epithelium (f) and (h).(g): Collecting ducts in kidney medulla were strongly stained with DIG-SLT-1B, as revealed by immunofluorescence. Bars represent 50 µm [(a) and (b), × 230 magnification] or 200 µm [(c) to (h), × 70 magnification].
    Fluorescent Marker, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent marker/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent marker - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    Lack of Shiga-like toxin (SLT)-binding sites in germinal centres of the lymph nodes and Peyer’s patches from ICR mice. Acetone-fixed frozen sections of normal mouse kidneys [(a), (b) and (g)], mouse draining lymph nodes 6 days after subcutaneous immunization [(c), (d) and (e)], or mouse Peyer’s patches 4 days after oral immunization [(f) and (h),], were stained with digoxigenin-labelled SLT-1B proteins (DIG-SLT-1B) plus horseradish peroxidase (HRP)-anti-DIG [(a) and (b)]. The sections were then counterstained with haematoxylin. The other sections were stained with haematoxylin and eosin (c); biotin-conjugated peanut agglutinin (PNA) plus Texas Red avidin D (d) and (f); or DIG-SLT-1B plus fluorescein isothiocyanate (FITC)-anti-DIG (e), (g) and (h).(a): Cortical regions of the kidney. Renal tubules were strongly stained with DIG-SLT-1B (arrowheads) while glomeruli were not stained (single arrow).(b): Medullary regions of the kidney. Cross-sections of collecting ducts were strongly stained with DIG-SLT-1B.(d), (e), (f) and (h): Germinal centres (arrows) were strongly stained with PNA but not with DIG-SLT-1B. Arrowheads point towards the intestinal epithelium (f) and (h).(g): Collecting ducts in kidney medulla were strongly stained with DIG-SLT-1B, as revealed by immunofluorescence. Bars represent 50 µm [(a) and (b), × 230 magnification] or 200 µm [(c) to (h), × 70 magnification].

    Journal: Immunology

    Article Title: Lack of Shiga-like toxin binding sites in germinal centres of mouse lymphoid tissues

    doi: 10.1046/j.1365-2567.2002.01373.x

    Figure Lengend Snippet: Lack of Shiga-like toxin (SLT)-binding sites in germinal centres of the lymph nodes and Peyer’s patches from ICR mice. Acetone-fixed frozen sections of normal mouse kidneys [(a), (b) and (g)], mouse draining lymph nodes 6 days after subcutaneous immunization [(c), (d) and (e)], or mouse Peyer’s patches 4 days after oral immunization [(f) and (h),], were stained with digoxigenin-labelled SLT-1B proteins (DIG-SLT-1B) plus horseradish peroxidase (HRP)-anti-DIG [(a) and (b)]. The sections were then counterstained with haematoxylin. The other sections were stained with haematoxylin and eosin (c); biotin-conjugated peanut agglutinin (PNA) plus Texas Red avidin D (d) and (f); or DIG-SLT-1B plus fluorescein isothiocyanate (FITC)-anti-DIG (e), (g) and (h).(a): Cortical regions of the kidney. Renal tubules were strongly stained with DIG-SLT-1B (arrowheads) while glomeruli were not stained (single arrow).(b): Medullary regions of the kidney. Cross-sections of collecting ducts were strongly stained with DIG-SLT-1B.(d), (e), (f) and (h): Germinal centres (arrows) were strongly stained with PNA but not with DIG-SLT-1B. Arrowheads point towards the intestinal epithelium (f) and (h).(g): Collecting ducts in kidney medulla were strongly stained with DIG-SLT-1B, as revealed by immunofluorescence. Bars represent 50 µm [(a) and (b), × 230 magnification] or 200 µm [(c) to (h), × 70 magnification].

    Article Snippet: Texas Red-avidin D, HRP-avidin D, biotin-conjugated peanut agglutinin (PNA) and 3-amino-9-ethylcarbazole (AEC) substrate kit were obtained from Vector (Burlingame, CA).

    Techniques: Binding Assay, Mouse Assay, Staining, Avidin-Biotin Assay, Immunofluorescence

    Characterization of anti-mouse S opsin ( A ) and M opsin ( B ) antibodies by Western blotting and immunohistochemistry. Western blot analysis was performed with normal adult C57BL/6J mouse whole retinal homogenate, and immunohistochemistry was done on C57BL/6J mouse retinal frozen sections. Cone photoreceptor cells were labeled with biotinylated peanut agglutinin and visualized with Texas Red-avidin D in red ( b,e ). S and M opsins were labeled with anti-S and anti-M opsin polyclonal antibodies, respectively, and visualized with fluorescein label in green ( a, d ). Dual immunofluorescence labeling verified both S and M opsin immunoreactivities localized to cone cells ( c, f ). OS, Outer segments; IS, inner segments; ONL, outer nuclear layer. Scale bar, 20 μm.

    Journal: The Journal of Neuroscience

    Article Title: GRK1-Dependent Phosphorylation of S and M Opsins and Their Binding to Cone Arrestin during Cone Phototransduction in the Mouse Retina

    doi: 10.1523/JNEUROSCI.23-14-06152.2003

    Figure Lengend Snippet: Characterization of anti-mouse S opsin ( A ) and M opsin ( B ) antibodies by Western blotting and immunohistochemistry. Western blot analysis was performed with normal adult C57BL/6J mouse whole retinal homogenate, and immunohistochemistry was done on C57BL/6J mouse retinal frozen sections. Cone photoreceptor cells were labeled with biotinylated peanut agglutinin and visualized with Texas Red-avidin D in red ( b,e ). S and M opsins were labeled with anti-S and anti-M opsin polyclonal antibodies, respectively, and visualized with fluorescein label in green ( a, d ). Dual immunofluorescence labeling verified both S and M opsin immunoreactivities localized to cone cells ( c, f ). OS, Outer segments; IS, inner segments; ONL, outer nuclear layer. Scale bar, 20 μm.

    Article Snippet: To visualize all cones, we incubated the slides with biotinylated peanut agglutinin (PNA; Vector Laboratories, Burlingame, CA) for 1 hr at room temperature (RT) and then with Texas Red-avidin D (Vector Laboratories) for 1 hr at RT.

    Techniques: Western Blot, Immunohistochemistry, Labeling, Avidin-Biotin Assay, Immunofluorescence