avidin biotin peroxidase complex  (Vector Laboratories)


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    Name:
    Avidin D Peroxidase labeled Av HRP Ready to Use R T U
    Description:
    Horseradish Peroxidase Avidin ready to use reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates Horseradish Peroxidase Avidin D HRP Avidin D is produced by our own coupling procedure which preserves the high specific activity of the peroxidase Using biotinylated primary or secondary intermediates and peroxidase labeled Avidin D antigens can be localized in histological sections cytospin preparations or smears Horseradish Peroxidase Avidin D is offered as a concentrate 5 mg ml or in a ready to use R T U stabilized solution 100ml 1 g ml in a bottle fitted with a drop dispenser top
    Catalog Number:
    a-2704
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    None
    Category:
    Proteins
    Size:
    100 ml
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    Structured Review

    Vector Laboratories avidin biotin peroxidase complex
    Avidin D Peroxidase labeled Av HRP Ready to Use R T U
    Horseradish Peroxidase Avidin ready to use reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates Horseradish Peroxidase Avidin D HRP Avidin D is produced by our own coupling procedure which preserves the high specific activity of the peroxidase Using biotinylated primary or secondary intermediates and peroxidase labeled Avidin D antigens can be localized in histological sections cytospin preparations or smears Horseradish Peroxidase Avidin D is offered as a concentrate 5 mg ml or in a ready to use R T U stabilized solution 100ml 1 g ml in a bottle fitted with a drop dispenser top
    https://www.bioz.com/result/avidin biotin peroxidase complex/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1
    Article Snippet: RWI ESKHKS DFGKFVLSS (the TSP-1 binding site in calreticulin) and a scrambled control peptide (RSVWIKKELGSKDSFHSF) were synthesized by the Biomolecular Resource Facility (University of Lund, Lund, Sweden). .. Biotinylated peroxidase and avidin were from Vector Laboratories (Burlingame, CA). .. Blood lymphocytes were purified using Lymphoprep and depleted of monocytes by treatment with carbonyl iron and magnetic removal of phagocytic cells.

    Article Title: In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer's disease
    Article Snippet: With rabbit primary antibodies, sections were treated with Vectastain blocking serum and labeled with one antibody or two. .. Single-labeled sections were incubated with primary antibody (30 min), M.O.M. anti-mouse, or Vectastain anti-rabbit biotinylated antisera (10 min), followed by avidin conjugated to peroxidase (5 min) and DAB as chromagen (2 min, Vector Laboratories), or avidin-FITC conjugate, or Texas red (5 min). .. Double-labeled sections were incubated with first antibody as above.

    Article Title: PINCH: More Than Just an Adaptor Protein in Cellular Response
    Article Snippet: Formalin fixed, paraffin embedded human brain samples from normal, low-grade astrocytoma and glioblastoma multiforme (US BIOmax, Inc., Rockville, MD) were histologically classified according to the World Health Organization Classification of Tumors of the Nervous System. .. Immunohistochemistry was performed using the avidin–biotin–peroxidase complex system, according to the manufacturer's instructions (Vectastain Elite ABC Peroxidase Kit; Vector Laboratories, Burlingame, CA). ..

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: Cheongsangbangpung-tang ameliorated the acute inflammatory response via the inhibition of NF-κB activation and MAPK phosphorylation
    Article Snippet: Histological process The dorsum and ventrum pedis skins of the hind paws were separated and fixed in 10% neutral buffered formalin then embedded in paraffin, sectioned (3 ~ 4 μm) and stained with haematoxylin and eosin (HE) to determine their general histopathological profiles and the immunohistochemistry for COX-2 and iNOS. .. Immunohistochemistry The changes in the immunoreactivities of COX-2 and iNOS were observed by purified primary antibodies with avidin-biotin-peroxidase (ABC) and peroxidase substrate kits (Vector Labs, Burlingame, CA, USA). .. Briefly, the endogenous peroxidase activity was blocked by incubation in methanol and 0.3% H2 O2 for 30 min, while non-specific binding of immunoglobulin was blocked with normal horse serum blocking solution for 1 h in a humidity chamber after heating (95–100 °C)-based epitope retrievals in 10 mM citrate buffers (pH: 6.0).

    Article Title: The ?-Chemokine Receptor D6 Is Expressed by Lymphatic Endothelium and a Subset of Vascular Tumors
    Article Snippet: .. The bound antibodies were detected using the Vector avidin-biotin-peroxidase detection system and Nova-Red substrate (Vector Laboratories, Peterborough, UK), according to the manufacturers instructions. .. Sections were fixed with neutral buffered formalin, counterstained with hematoxylin (Surgipath Europe Ltd., Peterborough, UK), dehydrated through increasing concentrations of ethanol, cleared in Histoclear, and mounted in Histomount (Fisher Scientific).

    Article Title: Estrogen Receptor β-Mediated Nuclear Interaction Between IRS-1 and Rad51 Inhibits Homologous Recombination Directed DNA Repair in Medulloblastoma
    Article Snippet: The formalin-fixed, paraffin embedded samples were sectioned at 4 μm in thickness, stained with Hematoxylin and Eosin and histologically classified according to the World Health Organization Classification of Tumors of the Nervous System. .. Immunohistochemistry was performed using the avidin–biotin–peroxidase complex system, according to the manufacturer’s instructions (Vectastain Elite ABC Peroxidase Kit; Vector Laboratories, Burlingame, CA). ..

    Article Title: Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy
    Article Snippet: Endogenous biotin was blocked by Avidin/Biotin Blocking Kit (Vector Laboratories). .. The sections were then incubated with an appropriate concentration of primary antibody followed by detection using the avidin-biotinylated peroxidase complex method (ABC; Vector Laboratories). .. CD205 (DEC205) was detected by further amplification with biotinylated tyramide (PerkinElmer) according to the manufacturer's instructions.

    Incubation:

    Article Title: In vivo localization of human acetylcholinesterase-derived species in a β-sheet conformation at the core of senile plaques in Alzheimer's disease
    Article Snippet: With rabbit primary antibodies, sections were treated with Vectastain blocking serum and labeled with one antibody or two. .. Single-labeled sections were incubated with primary antibody (30 min), M.O.M. anti-mouse, or Vectastain anti-rabbit biotinylated antisera (10 min), followed by avidin conjugated to peroxidase (5 min) and DAB as chromagen (2 min, Vector Laboratories), or avidin-FITC conjugate, or Texas red (5 min). .. Double-labeled sections were incubated with first antibody as above.

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy
    Article Snippet: Endogenous biotin was blocked by Avidin/Biotin Blocking Kit (Vector Laboratories). .. The sections were then incubated with an appropriate concentration of primary antibody followed by detection using the avidin-biotinylated peroxidase complex method (ABC; Vector Laboratories). .. CD205 (DEC205) was detected by further amplification with biotinylated tyramide (PerkinElmer) according to the manufacturer's instructions.

    Immunohistochemistry:

    Article Title: PINCH: More Than Just an Adaptor Protein in Cellular Response
    Article Snippet: Formalin fixed, paraffin embedded human brain samples from normal, low-grade astrocytoma and glioblastoma multiforme (US BIOmax, Inc., Rockville, MD) were histologically classified according to the World Health Organization Classification of Tumors of the Nervous System. .. Immunohistochemistry was performed using the avidin–biotin–peroxidase complex system, according to the manufacturer's instructions (Vectastain Elite ABC Peroxidase Kit; Vector Laboratories, Burlingame, CA). ..

    Article Title: Cheongsangbangpung-tang ameliorated the acute inflammatory response via the inhibition of NF-κB activation and MAPK phosphorylation
    Article Snippet: Histological process The dorsum and ventrum pedis skins of the hind paws were separated and fixed in 10% neutral buffered formalin then embedded in paraffin, sectioned (3 ~ 4 μm) and stained with haematoxylin and eosin (HE) to determine their general histopathological profiles and the immunohistochemistry for COX-2 and iNOS. .. Immunohistochemistry The changes in the immunoreactivities of COX-2 and iNOS were observed by purified primary antibodies with avidin-biotin-peroxidase (ABC) and peroxidase substrate kits (Vector Labs, Burlingame, CA, USA). .. Briefly, the endogenous peroxidase activity was blocked by incubation in methanol and 0.3% H2 O2 for 30 min, while non-specific binding of immunoglobulin was blocked with normal horse serum blocking solution for 1 h in a humidity chamber after heating (95–100 °C)-based epitope retrievals in 10 mM citrate buffers (pH: 6.0).

    Article Title: Estrogen Receptor β-Mediated Nuclear Interaction Between IRS-1 and Rad51 Inhibits Homologous Recombination Directed DNA Repair in Medulloblastoma
    Article Snippet: The formalin-fixed, paraffin embedded samples were sectioned at 4 μm in thickness, stained with Hematoxylin and Eosin and histologically classified according to the World Health Organization Classification of Tumors of the Nervous System. .. Immunohistochemistry was performed using the avidin–biotin–peroxidase complex system, according to the manufacturer’s instructions (Vectastain Elite ABC Peroxidase Kit; Vector Laboratories, Burlingame, CA). ..

    Purification:

    Article Title: Cheongsangbangpung-tang ameliorated the acute inflammatory response via the inhibition of NF-κB activation and MAPK phosphorylation
    Article Snippet: Histological process The dorsum and ventrum pedis skins of the hind paws were separated and fixed in 10% neutral buffered formalin then embedded in paraffin, sectioned (3 ~ 4 μm) and stained with haematoxylin and eosin (HE) to determine their general histopathological profiles and the immunohistochemistry for COX-2 and iNOS. .. Immunohistochemistry The changes in the immunoreactivities of COX-2 and iNOS were observed by purified primary antibodies with avidin-biotin-peroxidase (ABC) and peroxidase substrate kits (Vector Labs, Burlingame, CA, USA). .. Briefly, the endogenous peroxidase activity was blocked by incubation in methanol and 0.3% H2 O2 for 30 min, while non-specific binding of immunoglobulin was blocked with normal horse serum blocking solution for 1 h in a humidity chamber after heating (95–100 °C)-based epitope retrievals in 10 mM citrate buffers (pH: 6.0).

    Plasmid Preparation:

    Article Title: The ?-Chemokine Receptor D6 Is Expressed by Lymphatic Endothelium and a Subset of Vascular Tumors
    Article Snippet: .. The bound antibodies were detected using the Vector avidin-biotin-peroxidase detection system and Nova-Red substrate (Vector Laboratories, Peterborough, UK), according to the manufacturers instructions. .. Sections were fixed with neutral buffered formalin, counterstained with hematoxylin (Surgipath Europe Ltd., Peterborough, UK), dehydrated through increasing concentrations of ethanol, cleared in Histoclear, and mounted in Histomount (Fisher Scientific).

    Concentration Assay:

    Article Title: Induction of Tumor-specific T Cell Immunity by Anti-DR5 Antibody Therapy
    Article Snippet: Endogenous biotin was blocked by Avidin/Biotin Blocking Kit (Vector Laboratories). .. The sections were then incubated with an appropriate concentration of primary antibody followed by detection using the avidin-biotinylated peroxidase complex method (ABC; Vector Laboratories). .. CD205 (DEC205) was detected by further amplification with biotinylated tyramide (PerkinElmer) according to the manufacturer's instructions.

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    Vector Laboratories avidin biotin peroxidase complex abc
    Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the <t>ABC</t> method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a <t>chlamydial</t> inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).
    Avidin Biotin Peroxidase Complex Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex abc/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Vector Laboratories avidin biotin complex horseradish peroxidase immunostain techniques
    Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of <t>immunostain</t> intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]
    Avidin Biotin Complex Horseradish Peroxidase Immunostain Techniques, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex horseradish peroxidase immunostain techniques/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex horseradish peroxidase immunostain techniques - by Bioz Stars, 2021-03
    99/100 stars
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    99
    Vector Laboratories avidin biotin immunoperoxidase technique
    Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin <t>immunoperoxidase</t>
    Avidin Biotin Immunoperoxidase Technique, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin immunoperoxidase technique/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin immunoperoxidase technique - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a chlamydial inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).

    Journal: Infection and Immunity

    Article Title: Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion

    doi:

    Figure Lengend Snippet: Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a chlamydial inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).

    Article Snippet: To visualize chlamydial antigen in paraffin sections, immunohistochemical staining was carried out with a chlamydial-lipopolysaccharide-specific biotinylated mouse MAb, as previously described , by using the avidin-biotin-peroxidase complex (ABC) method according to the instructions of the manufacturer (Vector Laboratories, Burlingame, Calif.).

    Techniques: Histopathology, Mouse Assay, Infection

    Histopathology and immunoreaction in the livers of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions in the livers of depleted (a and c) and nondepleted (b and d) pregnant mice. (a) Liver of a depleted mouse at 3 days p.i. showing several chlamydial inclusions (notice the lack of leukocytes); (b) liver of a nondepleted mouse at 3 days p.i. showing an infectious focus, formed mainly by neutrophils, with a positive immunoreaction (notice that there are not typical chlamydial inclusions); (c) liver of a depleted mouse at 7 days p.i. showing numerous immunoreactive foci; (d) liver of a nondepleted mouse at 7 days p.i. showing extensive leukocyte infiltration (notice the lack of immunoreactivity in the foci). Magnifications, ×200 (a and b) and ×100 (c and d).

    Journal: Infection and Immunity

    Article Title: Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion

    doi:

    Figure Lengend Snippet: Histopathology and immunoreaction in the livers of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions in the livers of depleted (a and c) and nondepleted (b and d) pregnant mice. (a) Liver of a depleted mouse at 3 days p.i. showing several chlamydial inclusions (notice the lack of leukocytes); (b) liver of a nondepleted mouse at 3 days p.i. showing an infectious focus, formed mainly by neutrophils, with a positive immunoreaction (notice that there are not typical chlamydial inclusions); (c) liver of a depleted mouse at 7 days p.i. showing numerous immunoreactive foci; (d) liver of a nondepleted mouse at 7 days p.i. showing extensive leukocyte infiltration (notice the lack of immunoreactivity in the foci). Magnifications, ×200 (a and b) and ×100 (c and d).

    Article Snippet: To visualize chlamydial antigen in paraffin sections, immunohistochemical staining was carried out with a chlamydial-lipopolysaccharide-specific biotinylated mouse MAb, as previously described , by using the avidin-biotin-peroxidase complex (ABC) method according to the instructions of the manufacturer (Vector Laboratories, Burlingame, Calif.).

    Techniques: Histopathology, Mouse Assay, Infection

    Levels of TMEV in the brain and spinal cord of 3D transgenic mice. (A) Viral transcripts were quantified by real-time RT-PCR by using SYBR green. Primers specific for VP2 were used. Viral transcripts calculated from nonspecific amplification in uninfected FVB mice were divided by the viral transcripts of infected mice to set the baseline sensitivity of the assay to 1. White bars show values of infected nontransgenic mice, and dark bars show values of 3D transgenic mice. Data are shown as means ± SEM of five mice per group. WT, wild type. (B) Percentages of spinal cord quadrants containing TMEV-positive cells in the white matter are shown after 45 and 270 dpi by the same immunoperoxidase staining technique as that used for the brain. Each point represents one mouse. (C) Correlation of PFU from plaque assay and relative viral transcripts by real-time RT-PCR after the infection of SQSV cells with TMEV for 8, 16, and 24 h. The same VP2-specific primers were used as those for panel A. Each point represents data obtained from separate replicate samples for both assays.

    Journal: Journal of Virology

    Article Title: Transgenic Expression of the 3D Polymerase Inhibits Theiler's Virus Infection and Demyelination ▿Transgenic Expression of the 3D Polymerase Inhibits Theiler's Virus Infection and Demyelination ▿ †

    doi: 10.1128/JVI.00664-09

    Figure Lengend Snippet: Levels of TMEV in the brain and spinal cord of 3D transgenic mice. (A) Viral transcripts were quantified by real-time RT-PCR by using SYBR green. Primers specific for VP2 were used. Viral transcripts calculated from nonspecific amplification in uninfected FVB mice were divided by the viral transcripts of infected mice to set the baseline sensitivity of the assay to 1. White bars show values of infected nontransgenic mice, and dark bars show values of 3D transgenic mice. Data are shown as means ± SEM of five mice per group. WT, wild type. (B) Percentages of spinal cord quadrants containing TMEV-positive cells in the white matter are shown after 45 and 270 dpi by the same immunoperoxidase staining technique as that used for the brain. Each point represents one mouse. (C) Correlation of PFU from plaque assay and relative viral transcripts by real-time RT-PCR after the infection of SQSV cells with TMEV for 8, 16, and 24 h. The same VP2-specific primers were used as those for panel A. Each point represents data obtained from separate replicate samples for both assays.

    Article Snippet: Following incubation with a secondary biotinylated antibody (Vector Laboratories, Burlingame, CA), immunoreactivity was detected using the avidin-biotin immunoperoxidase technique (Vector Laboratories).

    Techniques: Transgenic Assay, Mouse Assay, Quantitative RT-PCR, SYBR Green Assay, Amplification, Infection, Immunoperoxidase Staining, Plaque Assay

    Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of immunostain intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]

    Journal: Journal of neuroscience research

    Article Title: Spatially Patterned Gene Expression for Guided Neurite Extension

    doi: 10.1002/jnr.21908

    Figure Lengend Snippet: Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of immunostain intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]

    Article Snippet: At the end of the culture period, HEK293T cells were fixed with 2% paraformaldehyde in PBS for 10 min. HEK293T cells were stained for NGF with anti-NGF-β (Millipore) and avidin–biotin complex–horseradish peroxidase immunostain techniques (Vector Labs; Burlingame, CA).

    Techniques: Immunocytochemistry, Cell Culture, Negative Control

    Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin immunoperoxidase

    Journal:

    Article Title: Increased Epitope-Specific CD8+ T Cells Prevent Murine Coronavirus Spread to the Spinal Cord and Subsequent Demyelination

    doi: 10.1128/JVI.79.6.3370-3381.2005

    Figure Lengend Snippet: Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin immunoperoxidase

    Article Snippet: Antigen staining was performed by the avidin-biotin-immunoperoxidase technique (Vector Laboratories, Burlingame, Calif.) by using diaminobenzidine tetrahydrochloride as the substrate and a 1:20 dilution of rabbit antinucleocapsid monoclonal antibody (kindly provided by Julian Leibowitz).

    Techniques: Infection, Mouse Assay, Staining, Avidin-Biotin Assay