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Santa Cruz Biotechnology avidin biotin peroxidase complex
Avidin Biotin Peroxidase Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avidin biotin peroxidase complex/product/Santa Cruz Biotechnology
Average 92 stars, based on 24 article reviews
Price from $9.99 to $1999.99
avidin biotin peroxidase complex - by Bioz Stars, 2020-09
92/100 stars

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Immunohistochemistry:

Article Title: Clinicopathologic features and treatment of breast metastasis from nasopharyngeal carcinoma: A report of two cases and literature review
Article Snippet: .. Immunohistochemistry (IHC) was performed on paraffin-embedded sections using the avidin-biotin-peroxidase complex method , with antibodies for human estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). .. In situ hybridization (ISH) for Epstein-Barr virus (EBV)-encoded small RNAs (EBER) was performed on paraffin-embedded sections, according to the manufacturer's instructions (Dako, Glostrup, Denmark).

Article Title: Heterogeneous Nuclear Ribonucleoprotein F Suppresses Angiotensinogen Gene Expression and Attenuates Hypertension and Kidney Injury in Diabetic Mice
Article Snippet: .. Immunohistochemical staining was performed by the standard avidin-biotin-peroxidase complex method using four to five sections per kidney and three mouse kidneys per group (ABC Staining System; Santa Cruz Biotechnology) ( – ). ..

Article Title: Primary lymphoepithelioma-like carcinoma of ocular adnexa: clinicopathologic features and treatment
Article Snippet: .. Immunohistochemistry ( ihc ) was performed on paraffin-embedded sections using the avidin–biotin–peroxidase complex method, with antibodies for cytokeratin, cytokeratin 19, and P63 (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.). .. In situ hybridization ( ish ) for ebv -encoded small rna s ( eber s) was performed on paraffin-embedded sections according to the manufacturer’s instructions (Dako, Glostrup, Denmark).

Avidin-Biotin Assay:

Article Title: Clinicopathologic features and treatment of breast metastasis from nasopharyngeal carcinoma: A report of two cases and literature review
Article Snippet: .. Immunohistochemistry (IHC) was performed on paraffin-embedded sections using the avidin-biotin-peroxidase complex method , with antibodies for human estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). .. In situ hybridization (ISH) for Epstein-Barr virus (EBV)-encoded small RNAs (EBER) was performed on paraffin-embedded sections, according to the manufacturer's instructions (Dako, Glostrup, Denmark).

Article Title: Effect of cyclin G2 on proliferative ability of SGC-7901 cell
Article Snippet: .. Cytochemistry staining was preformed by the avidin-biotin peroxidase complex method using the SABC kit as follows: cells were treated to remove endogenous peroxidase activity, blocked with rabbit serum for 30 min at room temperature, and then incubated in primary goat anti-cyclin G2 polyclonal antibody (American Santa Cruz Company) for 1 h at 37 °C or overnight at 4 °C, washed 3 times with PBS, then incubated with a rabbit-anti-goat secondary antiboby conjugated to horseradish peroxidase for 1 h. Following PBS washing, the coverslips were incubated with SABC elite reagent for 30 min at room temperature and washed 3 times in PBS. ..

Article Title: Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase-deficient Mice but not in Mast Cell-deficient Mice
Article Snippet: .. The slides were then incubated with goat polyclonal anti-CD31 (2 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h. After being washed with PBS, they were incubated with biotinylated anti–goat IgG (1 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h and then with the avidin-biotin peroxidase complex (1:500; Santa Cruz Biotechnology, Inc.) in PBS at room temperature for 30 min. .. The reaction product was detected with freshly prepared peroxidase substrate mixture containing 1.6 ml distilled water, five drops 10× substrate buffer, 1 drop 50 × 3,3′-diaminobenzidine tetrahydrochloride chromogen, and 1 drop 50× peroxidase substrate (Santa Cruz Biotechnology, Inc.).

Article Title: Expression of insulin receptor substrate 1 in primary breast cancer and lymph node metastases
Article Snippet: .. The IRS-1 studies were performed using the avidin–biotin–peroxidase complex (ABC staining system; Santa Cruz Biotechnology) and 3,3′-diaminobenzidine as chromogen. .. The expression of IRS-1 was estimated by light microscopy in 10 different tumour fields and the mean percentage of IRS-1 positive tumour cells was scored.

Article Title: Early Production of IL-22 but Not IL-17 by Peripheral Blood Mononuclear Cells Exposed to live Borrelia burgdorferi: The Role of Monocytes and Interleukin-1
Article Snippet: .. Biotinylated-goat anti-rabbit antibody and the avidin-biotin-peroxidase complex (ABC-system, Santa Cruz) and 3,3-diaminobenzidine-tetra-hydrochloride pellets (Sigma-Aldrich) were used for detection. ..

Article Title: Relationships between hypoxia markers and the leptin system, estrogen receptors in human primary and metastatic breast cancer: effects of preoperative chemotherapy
Article Snippet: .. The analysis of leptin, ObR, ERα and ERβ was performed with avidin-biotin-peroxidase complex (ABC Staining System, Santa Cruz, USA), for HIF-1α and Glut-1 with EnVision method (Dako, Denmark), and for Ki-67 with streptavidin-biotin-peroxidase complex (LSAB kit, Dako, Denmark) to reveal Ab-antigen reactions. .. Breast specimens previously classified as positive for the expression of the studied markers were used for control and protocol standardization.

Article Title: Heterogeneous Nuclear Ribonucleoprotein F Suppresses Angiotensinogen Gene Expression and Attenuates Hypertension and Kidney Injury in Diabetic Mice
Article Snippet: .. Immunohistochemical staining was performed by the standard avidin-biotin-peroxidase complex method using four to five sections per kidney and three mouse kidneys per group (ABC Staining System; Santa Cruz Biotechnology) ( – ). ..

Article Title: Primary lymphoepithelioma-like carcinoma of ocular adnexa: clinicopathologic features and treatment
Article Snippet: .. Immunohistochemistry ( ihc ) was performed on paraffin-embedded sections using the avidin–biotin–peroxidase complex method, with antibodies for cytokeratin, cytokeratin 19, and P63 (Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.). .. In situ hybridization ( ish ) for ebv -encoded small rna s ( eber s) was performed on paraffin-embedded sections according to the manufacturer’s instructions (Dako, Glostrup, Denmark).

Blocking Assay:

Article Title: Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase-deficient Mice but not in Mast Cell-deficient Mice
Article Snippet: .. The slides were then incubated with goat polyclonal anti-CD31 (2 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h. After being washed with PBS, they were incubated with biotinylated anti–goat IgG (1 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h and then with the avidin-biotin peroxidase complex (1:500; Santa Cruz Biotechnology, Inc.) in PBS at room temperature for 30 min. .. The reaction product was detected with freshly prepared peroxidase substrate mixture containing 1.6 ml distilled water, five drops 10× substrate buffer, 1 drop 50 × 3,3′-diaminobenzidine tetrahydrochloride chromogen, and 1 drop 50× peroxidase substrate (Santa Cruz Biotechnology, Inc.).

Incubation:

Article Title: Effect of cyclin G2 on proliferative ability of SGC-7901 cell
Article Snippet: .. Cytochemistry staining was preformed by the avidin-biotin peroxidase complex method using the SABC kit as follows: cells were treated to remove endogenous peroxidase activity, blocked with rabbit serum for 30 min at room temperature, and then incubated in primary goat anti-cyclin G2 polyclonal antibody (American Santa Cruz Company) for 1 h at 37 °C or overnight at 4 °C, washed 3 times with PBS, then incubated with a rabbit-anti-goat secondary antiboby conjugated to horseradish peroxidase for 1 h. Following PBS washing, the coverslips were incubated with SABC elite reagent for 30 min at room temperature and washed 3 times in PBS. ..

Article Title: Defective Angiogenesis in the Inflammatory Granulation Tissue in Histidine Decarboxylase-deficient Mice but not in Mast Cell-deficient Mice
Article Snippet: .. The slides were then incubated with goat polyclonal anti-CD31 (2 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h. After being washed with PBS, they were incubated with biotinylated anti–goat IgG (1 μg/ml; Santa Cruz Biotechnology, Inc.) in PBS containing 1.5% (wt/vol) blocking serum at room temperature for 1 h and then with the avidin-biotin peroxidase complex (1:500; Santa Cruz Biotechnology, Inc.) in PBS at room temperature for 30 min. .. The reaction product was detected with freshly prepared peroxidase substrate mixture containing 1.6 ml distilled water, five drops 10× substrate buffer, 1 drop 50 × 3,3′-diaminobenzidine tetrahydrochloride chromogen, and 1 drop 50× peroxidase substrate (Santa Cruz Biotechnology, Inc.).

Activity Assay:

Article Title: Effect of cyclin G2 on proliferative ability of SGC-7901 cell
Article Snippet: .. Cytochemistry staining was preformed by the avidin-biotin peroxidase complex method using the SABC kit as follows: cells were treated to remove endogenous peroxidase activity, blocked with rabbit serum for 30 min at room temperature, and then incubated in primary goat anti-cyclin G2 polyclonal antibody (American Santa Cruz Company) for 1 h at 37 °C or overnight at 4 °C, washed 3 times with PBS, then incubated with a rabbit-anti-goat secondary antiboby conjugated to horseradish peroxidase for 1 h. Following PBS washing, the coverslips were incubated with SABC elite reagent for 30 min at room temperature and washed 3 times in PBS. ..

Staining:

Article Title: Effect of cyclin G2 on proliferative ability of SGC-7901 cell
Article Snippet: .. Cytochemistry staining was preformed by the avidin-biotin peroxidase complex method using the SABC kit as follows: cells were treated to remove endogenous peroxidase activity, blocked with rabbit serum for 30 min at room temperature, and then incubated in primary goat anti-cyclin G2 polyclonal antibody (American Santa Cruz Company) for 1 h at 37 °C or overnight at 4 °C, washed 3 times with PBS, then incubated with a rabbit-anti-goat secondary antiboby conjugated to horseradish peroxidase for 1 h. Following PBS washing, the coverslips were incubated with SABC elite reagent for 30 min at room temperature and washed 3 times in PBS. ..

Article Title: Expression of insulin receptor substrate 1 in primary breast cancer and lymph node metastases
Article Snippet: .. The IRS-1 studies were performed using the avidin–biotin–peroxidase complex (ABC staining system; Santa Cruz Biotechnology) and 3,3′-diaminobenzidine as chromogen. .. The expression of IRS-1 was estimated by light microscopy in 10 different tumour fields and the mean percentage of IRS-1 positive tumour cells was scored.

Article Title: Relationships between hypoxia markers and the leptin system, estrogen receptors in human primary and metastatic breast cancer: effects of preoperative chemotherapy
Article Snippet: .. The analysis of leptin, ObR, ERα and ERβ was performed with avidin-biotin-peroxidase complex (ABC Staining System, Santa Cruz, USA), for HIF-1α and Glut-1 with EnVision method (Dako, Denmark), and for Ki-67 with streptavidin-biotin-peroxidase complex (LSAB kit, Dako, Denmark) to reveal Ab-antigen reactions. .. Breast specimens previously classified as positive for the expression of the studied markers were used for control and protocol standardization.

Article Title: Heterogeneous Nuclear Ribonucleoprotein F Suppresses Angiotensinogen Gene Expression and Attenuates Hypertension and Kidney Injury in Diabetic Mice
Article Snippet: .. Immunohistochemical staining was performed by the standard avidin-biotin-peroxidase complex method using four to five sections per kidney and three mouse kidneys per group (ABC Staining System; Santa Cruz Biotechnology) ( – ). ..

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  • 88
    Santa Cruz Biotechnology biotinylated horseradish peroxidase avidin complex
    Wnt pathway activation by extracellular Wnt3a as reflected in nuclear β-catenin content and level of Wnt throughput in EAhy926 endothelial cells . (A) Control cells were not treated with Wnt. (B) Cell treatment with Wnt3a increased their cytoplasmic and nuclear β-catenin content. Cells were analyzed by immunofluorescence with primary antibodies against β-catenin, <t>biotinylated</t> secondary antibody, and subsequently TSA Fluorescein System. (C) A typical control cell with β-catenin staining (green fluorescence) and nuclear staining (blue fluorescence, DAPI). (D) A typical Wnt3a-treated cell. After treatment of cells with Wnt3a conditioned media (CM) β-catenin staining co-localized with nuclei. (E) Ratio of nuclear to cytoplasmic β-catenin in the EAhy926 cells. Wnt3A CM increased the nuclear/cytoplasmic ratio (**p
    Biotinylated Horseradish Peroxidase Avidin Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horseradish peroxidase avidin complex/product/Santa Cruz Biotechnology
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotinylated horseradish peroxidase avidin complex - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology immunohistochemical avidin biotin peroxidase complex
    <t>Immunohistochemical</t> staining of PTTG1 and MMP proteins in laryngeal squamous cell carcinoma tissue and adjacent normal laryngeal tissue specimens. Positive PTTG1 immunostaining was predominantly localized in the cytoplasm of cancer cells. (A and B) Positive PTTG1 expression in laryngeal squamous cell carcinoma: magnification, (A) ×200 and (B) ×400. (C and D) Positive expression of (C) MMP-2 and (D) MMP-9 expression in laryngeal squamous cell carcinoma (magnification, ×200). (E) Negative PTTG1 expression in laryngeal squamous cell carcinoma (magnification, ×200). (F) Negative PTTG1 expression in normal laryngeal epithelium (magnification, ×400). PTTG1, pituitary tumor-transforming 1; MMP, matrix metalloproteinase.
    Immunohistochemical Avidin Biotin Peroxidase Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical avidin biotin peroxidase complex/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical avidin biotin peroxidase complex - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology avidin biotin peroxidase complex
    <t>Immunohistochemical</t> staining of PTTG1 and MMP proteins in laryngeal squamous cell carcinoma tissue and adjacent normal laryngeal tissue specimens. Positive PTTG1 immunostaining was predominantly localized in the cytoplasm of cancer cells. (A and B) Positive PTTG1 expression in laryngeal squamous cell carcinoma: magnification, (A) ×200 and (B) ×400. (C and D) Positive expression of (C) MMP-2 and (D) MMP-9 expression in laryngeal squamous cell carcinoma (magnification, ×200). (E) Negative PTTG1 expression in laryngeal squamous cell carcinoma (magnification, ×200). (F) Negative PTTG1 expression in normal laryngeal epithelium (magnification, ×400). PTTG1, pituitary tumor-transforming 1; MMP, matrix metalloproteinase.
    Avidin Biotin Peroxidase Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin peroxidase complex/product/Santa Cruz Biotechnology
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology avidin biotin horseradish peroxidase complex
    <t>Immunohistochemical</t> staining of PTTG1 and MMP proteins in laryngeal squamous cell carcinoma tissue and adjacent normal laryngeal tissue specimens. Positive PTTG1 immunostaining was predominantly localized in the cytoplasm of cancer cells. (A and B) Positive PTTG1 expression in laryngeal squamous cell carcinoma: magnification, (A) ×200 and (B) ×400. (C and D) Positive expression of (C) MMP-2 and (D) MMP-9 expression in laryngeal squamous cell carcinoma (magnification, ×200). (E) Negative PTTG1 expression in laryngeal squamous cell carcinoma (magnification, ×200). (F) Negative PTTG1 expression in normal laryngeal epithelium (magnification, ×400). PTTG1, pituitary tumor-transforming 1; MMP, matrix metalloproteinase.
    Avidin Biotin Horseradish Peroxidase Complex, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin horseradish peroxidase complex/product/Santa Cruz Biotechnology
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    avidin biotin horseradish peroxidase complex - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Wnt pathway activation by extracellular Wnt3a as reflected in nuclear β-catenin content and level of Wnt throughput in EAhy926 endothelial cells . (A) Control cells were not treated with Wnt. (B) Cell treatment with Wnt3a increased their cytoplasmic and nuclear β-catenin content. Cells were analyzed by immunofluorescence with primary antibodies against β-catenin, biotinylated secondary antibody, and subsequently TSA Fluorescein System. (C) A typical control cell with β-catenin staining (green fluorescence) and nuclear staining (blue fluorescence, DAPI). (D) A typical Wnt3a-treated cell. After treatment of cells with Wnt3a conditioned media (CM) β-catenin staining co-localized with nuclei. (E) Ratio of nuclear to cytoplasmic β-catenin in the EAhy926 cells. Wnt3A CM increased the nuclear/cytoplasmic ratio (**p

    Journal: Vascular Cell

    Article Title: Lymphoid enhancer-binding factor 1, a representative of vertebrate-specific Lef1/Tcf1 sub-family, is a Wnt-beta-catenin pathway target gene in human endothelial cells which regulates matrix metalloproteinase-2 expression and promotes endothelial cell invasion

    doi: 10.1186/2045-824X-3-28

    Figure Lengend Snippet: Wnt pathway activation by extracellular Wnt3a as reflected in nuclear β-catenin content and level of Wnt throughput in EAhy926 endothelial cells . (A) Control cells were not treated with Wnt. (B) Cell treatment with Wnt3a increased their cytoplasmic and nuclear β-catenin content. Cells were analyzed by immunofluorescence with primary antibodies against β-catenin, biotinylated secondary antibody, and subsequently TSA Fluorescein System. (C) A typical control cell with β-catenin staining (green fluorescence) and nuclear staining (blue fluorescence, DAPI). (D) A typical Wnt3a-treated cell. After treatment of cells with Wnt3a conditioned media (CM) β-catenin staining co-localized with nuclei. (E) Ratio of nuclear to cytoplasmic β-catenin in the EAhy926 cells. Wnt3A CM increased the nuclear/cytoplasmic ratio (**p

    Article Snippet: Incubation with the primary antibody was followed by incubation with biotinylated secondary antibody (final concentration 1µg/ml), and subsequently with biotinylated horseradish peroxidase-avidin complex (Santa Cruz biotechnology, Santa Cruz, CA).

    Techniques: Activation Assay, Immunofluorescence, Staining, Fluorescence

    Immunohistochemical staining of PTTG1 and MMP proteins in laryngeal squamous cell carcinoma tissue and adjacent normal laryngeal tissue specimens. Positive PTTG1 immunostaining was predominantly localized in the cytoplasm of cancer cells. (A and B) Positive PTTG1 expression in laryngeal squamous cell carcinoma: magnification, (A) ×200 and (B) ×400. (C and D) Positive expression of (C) MMP-2 and (D) MMP-9 expression in laryngeal squamous cell carcinoma (magnification, ×200). (E) Negative PTTG1 expression in laryngeal squamous cell carcinoma (magnification, ×200). (F) Negative PTTG1 expression in normal laryngeal epithelium (magnification, ×400). PTTG1, pituitary tumor-transforming 1; MMP, matrix metalloproteinase.

    Journal: Oncology Letters

    Article Title: Pituitary tumor-transforming 1 expression in laryngeal cancer and its association with prognosis

    doi: 10.3892/ol.2018.8745

    Figure Lengend Snippet: Immunohistochemical staining of PTTG1 and MMP proteins in laryngeal squamous cell carcinoma tissue and adjacent normal laryngeal tissue specimens. Positive PTTG1 immunostaining was predominantly localized in the cytoplasm of cancer cells. (A and B) Positive PTTG1 expression in laryngeal squamous cell carcinoma: magnification, (A) ×200 and (B) ×400. (C and D) Positive expression of (C) MMP-2 and (D) MMP-9 expression in laryngeal squamous cell carcinoma (magnification, ×200). (E) Negative PTTG1 expression in laryngeal squamous cell carcinoma (magnification, ×200). (F) Negative PTTG1 expression in normal laryngeal epithelium (magnification, ×400). PTTG1, pituitary tumor-transforming 1; MMP, matrix metalloproteinase.

    Article Snippet: The expression of PTTG1, MMP-2, and MMP-9 in laryngeal cancer normal cancer-adjacent laryngeal tissue were determined using an immunohistochemical avidin-biotin peroxidase complex method, according to the manufacturer's protocols (Santa Cruz Biotechnology, Inc. and Beijing ZhongShan Golden Bridge Biological Technology Co., Ltd., Beijing, China).

    Techniques: Immunohistochemistry, Staining, Immunostaining, Expressing