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GE Healthcare avidin biotin peroxidase complex
Avidin Biotin Peroxidase Complex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avidin biotin peroxidase complex/product/GE Healthcare
Average 99 stars, based on 5 article reviews
Price from $9.99 to $1999.99
avidin biotin peroxidase complex - by Bioz Stars, 2020-09
99/100 stars

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Western Blot:

Article Title: A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins
Article Snippet: .. Electrophoresed proteins were transferred to a nitrocellulose membrane (Bio-Rad) where biotinylated proteins were detected in Western blots with streptavidin-conjugated peroxidase (1:500) and visualized with enhanced chemiluminescence (Amersham). .. The same membrane was exposed to anti-Xpress monoclonal antibody (1:1,000) followed by alkaline phosphatase-conjugated anti-mouse secondary antibody at 1:1,000 and an NBT/BCIP substrate mix (Lifetech).

Incubation:

Article Title: Human Herpesvirus 8 Glycoprotein B (gB), gH, and gL Can Mediate Cell Fusion
Article Snippet: .. Subsequently, cells were sequentially incubated with biotinylated goat anti-mouse immunoglobulin G (IgG) conjugate (Sigma, St. Louis, Mo.) and streptavidin-horseradish peroxidase conjugate (Amersham Pharmacia Biotech, Piscataway, N.J.). .. After the addition of 50 mM phosphate-citrate buffer containing 0.03% sodium perborate and 3,3′,5,5′-tetramethylbenzidine (Sigma) dissolved in dimethyl sulfoxide, optical density (OD) readings at 370 nm were obtained with a plate spectrophotometer (SpectraMax 250; Molecular Devices, Sunnyvale, Calif.).

Article Title: Deep phosphoproteome analysis of Schistosoma mansoni leads development of a kinomic array that highlights sex-biased differences in adult worm protein phosphorylation
Article Snippet: .. 10 μl Phos-tag Biotin BTL-111 (1 mM aqueous solution; Wako Chemicals) was combined with 20 μl 1 mM ZnCl2 (Sigma), 1 μl HRP-streptavidin (GE Healthcare) and 469 μl TTBS and incubated at room temperature for 30 min. .. The solution was then subject to ultrafiltration through a NanoSep 30K Omega centrifugal filter (14,000 x g , 20 min; Pall Corporation) and the recovered Phos-tag biotin-streptavidin HRP complex diluted with 15 ml array wash buffer and stored at 4°C.

Article Title: LipL21 Is a Novel Surface-Exposed Lipoprotein of Pathogenic Leptospira Species
Article Snippet: .. The membrane was blocked overnight in 5% (wt/vol) skim milk buffer, washed twice in PBS-T, and incubated with streptavidin-horseradish peroxidase conjugate (Amersham) at a dilution of 1/20,000 in PBS-T for 30 min. .. The membrane was washed six times with PBS-T and developed with the enhanced chemiluminescence Western blot detection system before visualization with Hyperfilm (Amersham).

SDS Page:

Article Title: Neurite outgrowth on a fibronectin isoform expressed during peripheral nerve regeneration is mediated by the interaction of paxillin with ?4?1 integrins
Article Snippet: .. Precipitated cell surface biotin-labelled molecules were separated by SDS-PAGE under non-reducing conditions and detected with streptavidin-peroxidase followed by ECL (Amersham). ..

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    GE Healthcare biotinylated streptavidin peroxidase complex
    Conditional expression and localization of GFP-tagged RSV F protein in polarized epithelial MDCK cells. Transgenic MDCK cells were grown on porous filter supports for 3 days prior to the induction of either bF-GFP, hF-GFP, or gp40-GFP. (A) At the indicated times postinduction, cells were stained with a monoclonal antibody directed to β-catenin (red fluorescence) and analyzed by confocal laser scanning microscopy (GFP fluorescence in green). XZ scans of the cells are shown. (B) At the indicated times postinduction of bF-GFP or hF-GFP expression, cell surface proteins of either the apical (ap) or basolateral (ba) plasma membrane were labeled with sulfo-NHS-biotin. F-GFP was immunoprecipitated from cell lysates and analyzed by Western blotting using <t>streptavidin-peroxidase</t> for the detection of <t>biotinylated</t> F-GFP. The amounts of F-GFP were determined by densitometry and the percentages of apical or basolateral F-GFP of total F-GFP (apical plus basolateral F-GFP) were calculated. As a control for biotinylation efficacy, total cell lysates of biotinylated MDCK-(hF-GFP) cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a nitrocellulose membrane, and probed with streptavidin-peroxidase complex (lower panel).
    Biotinylated Streptavidin Peroxidase Complex, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated streptavidin peroxidase complex/product/GE Healthcare
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    biotinylated streptavidin peroxidase complex - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Conditional expression and localization of GFP-tagged RSV F protein in polarized epithelial MDCK cells. Transgenic MDCK cells were grown on porous filter supports for 3 days prior to the induction of either bF-GFP, hF-GFP, or gp40-GFP. (A) At the indicated times postinduction, cells were stained with a monoclonal antibody directed to β-catenin (red fluorescence) and analyzed by confocal laser scanning microscopy (GFP fluorescence in green). XZ scans of the cells are shown. (B) At the indicated times postinduction of bF-GFP or hF-GFP expression, cell surface proteins of either the apical (ap) or basolateral (ba) plasma membrane were labeled with sulfo-NHS-biotin. F-GFP was immunoprecipitated from cell lysates and analyzed by Western blotting using streptavidin-peroxidase for the detection of biotinylated F-GFP. The amounts of F-GFP were determined by densitometry and the percentages of apical or basolateral F-GFP of total F-GFP (apical plus basolateral F-GFP) were calculated. As a control for biotinylation efficacy, total cell lysates of biotinylated MDCK-(hF-GFP) cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a nitrocellulose membrane, and probed with streptavidin-peroxidase complex (lower panel).

    Journal: Journal of Virology

    Article Title: The Fusion Protein of Respiratory Syncytial Virus Triggers p53-Dependent Apoptosis

    doi: 10.1128/JVI.01887-07

    Figure Lengend Snippet: Conditional expression and localization of GFP-tagged RSV F protein in polarized epithelial MDCK cells. Transgenic MDCK cells were grown on porous filter supports for 3 days prior to the induction of either bF-GFP, hF-GFP, or gp40-GFP. (A) At the indicated times postinduction, cells were stained with a monoclonal antibody directed to β-catenin (red fluorescence) and analyzed by confocal laser scanning microscopy (GFP fluorescence in green). XZ scans of the cells are shown. (B) At the indicated times postinduction of bF-GFP or hF-GFP expression, cell surface proteins of either the apical (ap) or basolateral (ba) plasma membrane were labeled with sulfo-NHS-biotin. F-GFP was immunoprecipitated from cell lysates and analyzed by Western blotting using streptavidin-peroxidase for the detection of biotinylated F-GFP. The amounts of F-GFP were determined by densitometry and the percentages of apical or basolateral F-GFP of total F-GFP (apical plus basolateral F-GFP) were calculated. As a control for biotinylation efficacy, total cell lysates of biotinylated MDCK-(hF-GFP) cells were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a nitrocellulose membrane, and probed with streptavidin-peroxidase complex (lower panel).

    Article Snippet: After the membrane was incubated for 60 min with a biotinylated streptavidin-peroxidase complex (1:1,000; GE Healthcare), antigens were visualized by enhanced chemiluminescence (Super-Signal; Pierce).

    Techniques: Expressing, Transgenic Assay, Staining, Fluorescence, Confocal Laser Scanning Microscopy, Labeling, Immunoprecipitation, Western Blot, Polyacrylamide Gel Electrophoresis

    Analysis of hF-GFP-expressing MDCK cells for apoptotic markers. (A) MDCK cells were induced to express either hF-GFP or gp40-GFP for 48 h or were left uninduced. Genomic DNA was extracted from the adherent (a) and the floating (f) cell fractions of hF-GFP-expressing cells. Cells expressing gp40-GFP did not show any cell loss, and therefore DNA was only extracted from adherent cells. Extracted DNA was separated by gel electrophoresis and visualized by UV light transillumination after ethidium bromide staining. (B) MDCK cells were grown on coverslips for 24 h before hF-GFP expression was induced for 40 h or not induced. The cells were analyzed by fluorescence microscopy after DAPI (blue) or TUNEL (red) staining. GFP fluorescence is shown in green. Note that a large syncytium that was extruded from the monolayer is shown in the lower panels. (C) Expression of hF-GFP was induced for 48 h in the presence or absence of Synagis (5 μg/ml). Adherent and floating cells were pooled and stained with TUNEL reagent. The percentages of positive cells are shown. (D) A549 cells (5 × 10 5 cells) were infected with RSV A2 using an MOI of 3 PFU/cell, and hF-GFP expression was induced in MDCK cells (5 × 10 5 cells) using different concentrations of mifepristone (10 −9 M, 2.5 × 10 −10 M, and 0.6 × 10 −11 M). After 24 h, the surface proteins of A549 and MDCK cells were labeled with sulfo-NHS-LC-biotin, and F protein was immunoprecipitated from the cell lysates. Biotinylated F protein was detected by Western blotting using streptavidin-peroxidase as probe. The levels of F antigen were quantified by densitometry and are expressed as percentages of the amount of F antigen induced by 10 −9 M mifepristone. (E) The expression of hF-GFP was induced with different concentrations of mifepristone (see above). Adherent and floating cells were collected 48 h postinduction and stained with TUNEL reagent. The percentages of cells that reacted with the reagent are shown. (F) The caspase-3/7 activity was determined in MDCK cell lysates 48 h after induction of hF-GFP expression using different mifepristone concentrations (see above). (G) The caspase-3/7 activity was determined in cell lysates 24, 48, and 72 h after induction of hF-GFP with 10 −9 M mifepristone. (H) MDCK cells were induced to express either gp40-GFP or hF-GFP for 40 h in the presence or absence of the indicated inhibitors (z-VAD, z-IETD, and z-LEHD at 25 μM; Synagis at 5 μg/ml; Y-27632 at 5 μM; SB203580 at 5 μM; or IMD-0354 at 1 μM), and the caspase-3/7 activity was determined in cell lysates. (I) The release of LDH activity was determined 40 h postinduction of hF-GFP in the absence or presence of pan-caspase inhibitor z-VAD (25 μM). The measurement results shown in panels G, H, and I are expressed as the relative fold increase of enzyme activity compared to noninduced cells. Arithmetic means and standard deviations of three experiments are shown.

    Journal: Journal of Virology

    Article Title: The Fusion Protein of Respiratory Syncytial Virus Triggers p53-Dependent Apoptosis

    doi: 10.1128/JVI.01887-07

    Figure Lengend Snippet: Analysis of hF-GFP-expressing MDCK cells for apoptotic markers. (A) MDCK cells were induced to express either hF-GFP or gp40-GFP for 48 h or were left uninduced. Genomic DNA was extracted from the adherent (a) and the floating (f) cell fractions of hF-GFP-expressing cells. Cells expressing gp40-GFP did not show any cell loss, and therefore DNA was only extracted from adherent cells. Extracted DNA was separated by gel electrophoresis and visualized by UV light transillumination after ethidium bromide staining. (B) MDCK cells were grown on coverslips for 24 h before hF-GFP expression was induced for 40 h or not induced. The cells were analyzed by fluorescence microscopy after DAPI (blue) or TUNEL (red) staining. GFP fluorescence is shown in green. Note that a large syncytium that was extruded from the monolayer is shown in the lower panels. (C) Expression of hF-GFP was induced for 48 h in the presence or absence of Synagis (5 μg/ml). Adherent and floating cells were pooled and stained with TUNEL reagent. The percentages of positive cells are shown. (D) A549 cells (5 × 10 5 cells) were infected with RSV A2 using an MOI of 3 PFU/cell, and hF-GFP expression was induced in MDCK cells (5 × 10 5 cells) using different concentrations of mifepristone (10 −9 M, 2.5 × 10 −10 M, and 0.6 × 10 −11 M). After 24 h, the surface proteins of A549 and MDCK cells were labeled with sulfo-NHS-LC-biotin, and F protein was immunoprecipitated from the cell lysates. Biotinylated F protein was detected by Western blotting using streptavidin-peroxidase as probe. The levels of F antigen were quantified by densitometry and are expressed as percentages of the amount of F antigen induced by 10 −9 M mifepristone. (E) The expression of hF-GFP was induced with different concentrations of mifepristone (see above). Adherent and floating cells were collected 48 h postinduction and stained with TUNEL reagent. The percentages of cells that reacted with the reagent are shown. (F) The caspase-3/7 activity was determined in MDCK cell lysates 48 h after induction of hF-GFP expression using different mifepristone concentrations (see above). (G) The caspase-3/7 activity was determined in cell lysates 24, 48, and 72 h after induction of hF-GFP with 10 −9 M mifepristone. (H) MDCK cells were induced to express either gp40-GFP or hF-GFP for 40 h in the presence or absence of the indicated inhibitors (z-VAD, z-IETD, and z-LEHD at 25 μM; Synagis at 5 μg/ml; Y-27632 at 5 μM; SB203580 at 5 μM; or IMD-0354 at 1 μM), and the caspase-3/7 activity was determined in cell lysates. (I) The release of LDH activity was determined 40 h postinduction of hF-GFP in the absence or presence of pan-caspase inhibitor z-VAD (25 μM). The measurement results shown in panels G, H, and I are expressed as the relative fold increase of enzyme activity compared to noninduced cells. Arithmetic means and standard deviations of three experiments are shown.

    Article Snippet: After the membrane was incubated for 60 min with a biotinylated streptavidin-peroxidase complex (1:1,000; GE Healthcare), antigens were visualized by enhanced chemiluminescence (Super-Signal; Pierce).

    Techniques: Expressing, Nucleic Acid Electrophoresis, Staining, Fluorescence, Microscopy, TUNEL Assay, Infection, Labeling, Immunoprecipitation, Western Blot, Activity Assay, Radial Immuno Diffusion