avidin biotin peroxidase complex solution  (Millipore)


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    Name:
    Avidin Peroxidase
    Description:
    Avidin is homotetrameric protein 68kDa that is obtained from egg whites This egg protein binds strongly to biotin Thus avidin biotin association has been utilized in immunoassays to detect the localization of antigens in tissues The use of avidin biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities
    Catalog Number:
    a3151
    Price:
    None
    Applications:
    To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.
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    Structured Review

    Millipore avidin biotin peroxidase complex solution
    Avidin is homotetrameric protein 68kDa that is obtained from egg whites This egg protein binds strongly to biotin Thus avidin biotin association has been utilized in immunoassays to detect the localization of antigens in tissues The use of avidin biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities
    https://www.bioz.com/result/avidin biotin peroxidase complex solution/product/Millipore
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex solution - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: CXCR3-Dependent Plasma Blast Migration to the Central Nervous System during Viral Encephalomyelitis ▿
    Article Snippet: .. ASC were detected by sequential incubation with biotinylated rabbit anti-mouse IgG (0.5 μg/ml; Southern Biotech, Birmingham, AL) overnight at 4°C, avidin peroxidase (2 μg/ml; Sigma, St. Louis, MO) for 1 h at room temperature, and filtered 3,3′-diaminobenzidine substrate (∼3 min). .. Spots were counted using an ImmunoSpot ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH).

    Binding Assay:

    Article Title: A Protective Role for C5a in the Development of Allergic Asthma Associated with Altered Levels of B7-H1 and B7-DC on Plasmacytoid Dendritic Cells 1
    Article Snippet: .. Total IgE binding was detected using biotin-conjugated rat anti-mouse IgE mAb (BD Pharmingen), followed by avidin-peroxidase (Sigma-Aldrich), and one-step ABTS (Pierce) as substrates. .. To assess serum HDM-specific IgE levels, 50 μ l of HDM diluted in 0.1 M Na2 HPO4 (PH 8.2) at a concentration of 100 μ g/ml were used to coat the plates.

    Avidin-Biotin Assay:

    Article Title: Anti-Lubricin Monoclonal Antibodies Created Using Lubricin-Knockout Mice Immunodetect Lubricin in Several Species and in Patients with Healthy and Diseased Joints
    Article Snippet: .. Detection of the avidin/biotin/peroxidase-conjugated secondary antibody was performed using DAB FastTabs substrate (Sigma-Aldrich) for 5 minutes, followed by counterstaining with hematoxylin or hematoxylin and eosin. ..

    Article Title: DNA-MVA Vaccine Protection after X4 SHIV Challenge in Macaques Correlates with Day-of-Challenge Antiviral CD4+ Cell-Mediated Immunity Levels and Postchallenge Preservation of CD4+ T Cell Memory
    Article Snippet: .. Final development was with avidin-peroxidase and ABTS (Sigma), followed by measurement of absorbance at 414 nm. .. The endpoint titer of anti-SIV or gp120MN IgG antibody was determined as the last serial 2-fold dilution of plasma that produced an absorbance value that was greater than the mean absorbance plus 3 standard deviations of eight blank (buffer diluent) wells that were reacted with the same reagents.

    Article Title: A Protective Role for C5a in the Development of Allergic Asthma Associated with Altered Levels of B7-H1 and B7-DC on Plasmacytoid Dendritic Cells 1
    Article Snippet: .. Total IgE binding was detected using biotin-conjugated rat anti-mouse IgE mAb (BD Pharmingen), followed by avidin-peroxidase (Sigma-Aldrich), and one-step ABTS (Pierce) as substrates. .. To assess serum HDM-specific IgE levels, 50 μ l of HDM diluted in 0.1 M Na2 HPO4 (PH 8.2) at a concentration of 100 μ g/ml were used to coat the plates.

    Article Title: Anti-Toll-like receptor 2 and 4 antibodies suppress inflammatory response in mice
    Article Snippet: .. Anti-leishmanial antibody titres in the pooled sera ( n = 5) were detected with biotin-conjugated anti-mouse IgG1 or IgG2 (MD Biosciences, St. Paul, MN) followed by conjugated avidin peroxidase (Sigma) and developed with tetramethylbenzidine substrate (Kirkegard & Perry, Gaithersburg, MD). .. RNA was purified from tissue samples using the RNeasy Mini Kit following the manufacturer's instructions (Qiagen, Manchester, UK).

    Article Title: Differential development of oil granulomas induced by pristane injection in galectin-3 deficient mice
    Article Snippet: .. Primary antibodies were revealed with a biotinylated anti-rat IgG (BA-4001, Vector Laboratories, Burlingame, CA, USA) and developed with avidin-peroxidase (1:200 in PBS) (Sigma Aldrich, USA), using diaminobenzidyne as the chromogen. .. Cell suspensions Bone marrow cells were obtained ex vivo by flushing the femoral cavity of WT and gal-3−/− mice with phosphate buffered saline (PBS), pH 7.2, with 3 % fetal bovine serum (FBS, LGC; Cotia, São Paulo, Brazil).

    Article Title: CXCR3-Dependent Plasma Blast Migration to the Central Nervous System during Viral Encephalomyelitis ▿
    Article Snippet: .. ASC were detected by sequential incubation with biotinylated rabbit anti-mouse IgG (0.5 μg/ml; Southern Biotech, Birmingham, AL) overnight at 4°C, avidin peroxidase (2 μg/ml; Sigma, St. Louis, MO) for 1 h at room temperature, and filtered 3,3′-diaminobenzidine substrate (∼3 min). .. Spots were counted using an ImmunoSpot ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH).

    Article Title: Membrane Type-1 Matrix Metalloproteinase Expression in Acute Myeloid Leukemia and Its Upregulation by Tumor Necrosis Factor-?
    Article Snippet: .. For color development, avidin peroxidase conjugate and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS, both from Sigma) were used, and optical was density measured at 405 nm with background correction at 650 nm. .. Statistical Analysis Arithmetic means and standard deviations were calculated and statistical analysis of significance was carried out using a paired, 2-tailed Student’s t-test with 95% confidence interval.

    Article Title: Taraxacum officinale protects against cholecystokinin-induced acute pancreatitis in rats
    Article Snippet: .. Avidin-peroxidase and 2’-AZINO-bis (3-ethylbenzithiazoline-6-sulfonic acid) tablet substrate were purchased from Sigma (St. Louis, MO, USA). ..

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    Millipore streptavidin biotinylated hrp complex
    Swarm sensing with dropicles. (A) Histograms of fluorescence intensities for populations of dropicles formed from O-shaped particle design 2 after the QuantaRed assay readout at 45 min. Concentration of <t>streptavidin-HRP</t> ranges from 0 (control) to 1 nM. Results correlate with the mean intensity results reported in Figure 6C. (B) Normalized standard error of the mean vs number of dropicles in the sample for the amplified assay showing that a larger number of dropicles leads to a more accurate representation of the concentration of analyte.
    Streptavidin Biotinylated Hrp Complex, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin biotinylated hrp complex/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin biotinylated hrp complex - by Bioz Stars, 2020-09
    99/100 stars
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    90
    Millipore avidin biotin immunoperoxidase technique
    Swarm sensing with dropicles. (A) Histograms of fluorescence intensities for populations of dropicles formed from O-shaped particle design 2 after the QuantaRed assay readout at 45 min. Concentration of <t>streptavidin-HRP</t> ranges from 0 (control) to 1 nM. Results correlate with the mean intensity results reported in Figure 6C. (B) Normalized standard error of the mean vs number of dropicles in the sample for the amplified assay showing that a larger number of dropicles leads to a more accurate representation of the concentration of analyte.
    Avidin Biotin Immunoperoxidase Technique, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin immunoperoxidase technique/product/Millipore
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avidin biotin immunoperoxidase technique - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Swarm sensing with dropicles. (A) Histograms of fluorescence intensities for populations of dropicles formed from O-shaped particle design 2 after the QuantaRed assay readout at 45 min. Concentration of streptavidin-HRP ranges from 0 (control) to 1 nM. Results correlate with the mean intensity results reported in Figure 6C. (B) Normalized standard error of the mean vs number of dropicles in the sample for the amplified assay showing that a larger number of dropicles leads to a more accurate representation of the concentration of analyte.

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Swarm sensing with dropicles. (A) Histograms of fluorescence intensities for populations of dropicles formed from O-shaped particle design 2 after the QuantaRed assay readout at 45 min. Concentration of streptavidin-HRP ranges from 0 (control) to 1 nM. Results correlate with the mean intensity results reported in Figure 6C. (B) Normalized standard error of the mean vs number of dropicles in the sample for the amplified assay showing that a larger number of dropicles leads to a more accurate representation of the concentration of analyte.

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Fluorescence, Concentration Assay, Amplification

    Minimal cross talk is observed between reactions in separate dropicles over time. Microscopic images showing the same well at 15 min, 35 min, 60 min, and 48 hrs after initiating the QuantaRed reaction. Two types of particles (with and without biotin) are introduced and incubated with 0.1 nM of streptavidin-HRP.

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Minimal cross talk is observed between reactions in separate dropicles over time. Microscopic images showing the same well at 15 min, 35 min, 60 min, and 48 hrs after initiating the QuantaRed reaction. Two types of particles (with and without biotin) are introduced and incubated with 0.1 nM of streptavidin-HRP.

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Incubation

    Amplified bioassay in dropicles. (A) Schematic of the assay workflow for biotin streptavidin affinity and the mechanism of amplification using horse radish peroxidase (HRP) turnover of the fluorogenic substrate ADHP to generate resorufin. (B) Microscopic images of a single well at different steps of the assay workflow. Images are captured when particles are in ethanol (step 1), PBS (step 2), and PSDS oil (step 4) where dropicles are formed. Zoomed in inset images of the same field-of-view highlight the particle morphology changes as the inner PEG and outer PPG layers swell or shrink in different solutions. Scale bar for the whole well is 1 mm, and the scale bar for the insets is 100 μm.

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Amplified bioassay in dropicles. (A) Schematic of the assay workflow for biotin streptavidin affinity and the mechanism of amplification using horse radish peroxidase (HRP) turnover of the fluorogenic substrate ADHP to generate resorufin. (B) Microscopic images of a single well at different steps of the assay workflow. Images are captured when particles are in ethanol (step 1), PBS (step 2), and PSDS oil (step 4) where dropicles are formed. Zoomed in inset images of the same field-of-view highlight the particle morphology changes as the inner PEG and outer PPG layers swell or shrink in different solutions. Scale bar for the whole well is 1 mm, and the scale bar for the insets is 100 μm.

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Amplification

    Duplex assay using shape-coded particles demonstrating minimal cross talk. (A) Schematic of the duplex assay showing the two particle populations: plus-shaped particles without biotin in the PEGDA layer are used as negative control population; H-shaped particles with biotin in the PEGDA layer are used as a positive population. (B) A merged image of bright field and fluorescent channels, after mixing plus- and H-shaped particles, incubating with 0.1 nM streptavidin-HRP solution, and initiating the HRP amplification reaction. There is contrast in the red fluorescent signal between plus-shaped particles and H-shaped particles. (C) Fluorescence images of the same field of view as in (B) at 15, 35 and 60 min after initiating the reaction. Red dotted lines in the images outline the PPG boundary of H-particles (positive) while white dotted lines outline the PPG boundary of plus-particle (negative). Scale bars in (B-C) represent 100μm. (D) Histograms showing the intensity distribution for a population of plus-shaped and H-shaped dropicles at 15, 35 and 60 min. (E) The mean of fluorescent intensity at these three timepoints 15, 35, and 60 min for H- and plus-shaped particles, showing the negative volume does not appear to have an appreciable increase in intensity over time even as the positive volume shows high levels of fluorescence intensity increase. The error bars represent standard deviation.

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Duplex assay using shape-coded particles demonstrating minimal cross talk. (A) Schematic of the duplex assay showing the two particle populations: plus-shaped particles without biotin in the PEGDA layer are used as negative control population; H-shaped particles with biotin in the PEGDA layer are used as a positive population. (B) A merged image of bright field and fluorescent channels, after mixing plus- and H-shaped particles, incubating with 0.1 nM streptavidin-HRP solution, and initiating the HRP amplification reaction. There is contrast in the red fluorescent signal between plus-shaped particles and H-shaped particles. (C) Fluorescence images of the same field of view as in (B) at 15, 35 and 60 min after initiating the reaction. Red dotted lines in the images outline the PPG boundary of H-particles (positive) while white dotted lines outline the PPG boundary of plus-particle (negative). Scale bars in (B-C) represent 100μm. (D) Histograms showing the intensity distribution for a population of plus-shaped and H-shaped dropicles at 15, 35 and 60 min. (E) The mean of fluorescent intensity at these three timepoints 15, 35, and 60 min for H- and plus-shaped particles, showing the negative volume does not appear to have an appreciable increase in intensity over time even as the positive volume shows high levels of fluorescence intensity increase. The error bars represent standard deviation.

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Negative Control, Amplification, Fluorescence, Standard Deviation

    Amplified assay performance in dropicles. (A) Microscopic images of QuantaRed assay results using two O-shaped particle designs for two concentrations of streptavidin-HRP, i.e., negative control and 1 nM. The look-up table was adjusted to be the same for each condition for visibility. The scale bar represents 100 μm. (B-C) Mean amplified intensity across a population of dropicles as a function of concentration of streptavidin-HRP using (B) particle design 1 (N=11-38) and (C) particle design 2 (N=307-382), each tuned by varying the thickness ( t ) and width ( w ) of the internal PEG layer. (D) Mean amplified fluorescent intensity across a population of dropicles as a function of concentration of NT-proBNP. Data reported in (A-D) were obtained after 45 min of reaction. Error bars in (B-D) represent standard error. *** represents p

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Amplified assay performance in dropicles. (A) Microscopic images of QuantaRed assay results using two O-shaped particle designs for two concentrations of streptavidin-HRP, i.e., negative control and 1 nM. The look-up table was adjusted to be the same for each condition for visibility. The scale bar represents 100 μm. (B-C) Mean amplified intensity across a population of dropicles as a function of concentration of streptavidin-HRP using (B) particle design 1 (N=11-38) and (C) particle design 2 (N=307-382), each tuned by varying the thickness ( t ) and width ( w ) of the internal PEG layer. (D) Mean amplified fluorescent intensity across a population of dropicles as a function of concentration of NT-proBNP. Data reported in (A-D) were obtained after 45 min of reaction. Error bars in (B-D) represent standard error. *** represents p

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Amplification, Negative Control, Concentration Assay

    Time dependence of intensity change in dropicles using a QuantaRed assay. (A) Fluorescence signal increase over time from 10 to 45 min for binding of biotinylated particles with streptavidin-HRP at 10 pM and 100 fM concentrations. The control condition is the same particles and reagents without streptavidin-HRP. Data represents mean of intensity for dropicles with error bars representing standard error of the mean. Intensity drop at early time points is likely due to changes in shape/swelling upon introduction of the oil phase. (B-C) Fluorescence intensity from individual particles are shown over time for (B) 100 fM and (C) 10 pM concentrations of streptavidin-HRP respectively.

    Journal: bioRxiv

    Article Title: Formation of uniform reaction volumes using concentric amphiphilic microparticles

    doi: 10.1101/2020.03.15.992321

    Figure Lengend Snippet: Time dependence of intensity change in dropicles using a QuantaRed assay. (A) Fluorescence signal increase over time from 10 to 45 min for binding of biotinylated particles with streptavidin-HRP at 10 pM and 100 fM concentrations. The control condition is the same particles and reagents without streptavidin-HRP. Data represents mean of intensity for dropicles with error bars representing standard error of the mean. Intensity drop at early time points is likely due to changes in shape/swelling upon introduction of the oil phase. (B-C) Fluorescence intensity from individual particles are shown over time for (B) 100 fM and (C) 10 pM concentrations of streptavidin-HRP respectively.

    Article Snippet: For the biotin to streptavidin-HRP binding assays reported, the inner PEGDA layer is also biotinylated to enable binding of streptavidin.

    Techniques: Fluorescence, Binding Assay

    Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase (HRP)-conjugated secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and streptavidin-HRP (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.

    Journal: Pathogens

    Article Title: Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity

    doi: 10.3390/pathogens9050375

    Figure Lengend Snippet: Conception and applicability of autotransporter (AT)—complement receptor ligand (CRL) fusions. ( a ) Graphical abstract. ( b ) The modification of YadA to yield YFC (YadA-FLAG-C3d) and YFP (YadA-FLAG-p28); SS: signal sequence. F: FLAG tag. ( c ) Heterologous expression of plasmid-borne YadA by Ec and Ft detected by western blot with α-YadA sera followed by horseradish peroxidase (HRP)-conjugated secondary Ab. Strains bearing empty vector are denoted by “-”. Arrows indicate the trimers and monomers of unmodified YadA. ( d ) Collagen-coated ELISA plates were incubated with serial dilutions of intact Ft :-, Ft :YadA, and Ft :YFC; bound bacteria were detected by α- Ft LPS Ab. ( e , f ) Whole cell lysates of Ec , Kp , and Ft containing empty vector (-) or the YFP or YFC expression vectors were probed by western blot with primary Ab specific for C3d and the FLAG epitope followed sequentially by biotinylated secondary Abs and streptavidin-HRP (SA-HRP). YFC and YFP trimers and monomers are designated with black and grey arrows. The ~20 kDa bands evident in all lanes are endogenously biotinylated bacterial proteins (annotated AccB in Ec and Ft [ 40 ]) detected by SA-HRP. ( g ) Intact bacteria as indicated were incubated in solution with α-FLAG Ab. ( h ) Intact Ft as indicated were incubated in solution with the indicated Ab. IglC is primarily a cytoplasmic Ft protein. Washed bacteria in ( g , h ) were probed for IgG heavy chain (HC), followed by biotinylated secondary Abs and SA-HRP.

    Article Snippet: Secondary horseradish peroxidase (HRP)-conjugated antibodies were diluted to the indicated concentrations in PBST and incubated at 4 °C for 1 h. Where indicated, a biotinylated secondary antibody was used, followed by streptavidin-linked HRP, each for 1 h incubations at 4 °C.

    Techniques: Modification, Sequencing, FLAG-tag, Expressing, Plasmid Preparation, Western Blot, Enzyme-linked Immunosorbent Assay, Incubation