avidin biotin peroxidase complex method  (Vector Laboratories)


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    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    PK-6100
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    No antibody included
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin peroxidase complex method
    VECTASTAIN Elite ABC HRP Kit Peroxidase Standard
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 2 ml Reagent A2 ml Reagent BReagent A and B when mixed form the ABC peroxidase complex Kit contains sufficient reagents to stain 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/avidin biotin peroxidase complex method/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin peroxidase complex method - by Bioz Stars, 2021-06
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    Images

    1) Product Images from "Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats"

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    Journal: Viruses

    doi: 10.3390/v10120681

    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Figure Legend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Techniques Used: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    2) Product Images from "A Semiquantitative Scoring System for Histopathological and Immunohistochemical Assessment of Lesions and Tissue Tropism in Avian Influenza"

    Article Title: A Semiquantitative Scoring System for Histopathological and Immunohistochemical Assessment of Lesions and Tissue Tropism in Avian Influenza

    Journal: Viruses

    doi: 10.3390/v13050868

    Virus score: Immunohistological scoring of influenza A virus (IAV)-antigen distribution, avian influenza virus (AIV)-infected birds (bar = 100 µm, IAV-matrixprotein immunohistochemistry, avidin-biotin-peroxidase complex method, 3-amino-9-ethyl-carbazol as chromogen and hematoxylin counterstain; Nomarski contrast).
    Figure Legend Snippet: Virus score: Immunohistological scoring of influenza A virus (IAV)-antigen distribution, avian influenza virus (AIV)-infected birds (bar = 100 µm, IAV-matrixprotein immunohistochemistry, avidin-biotin-peroxidase complex method, 3-amino-9-ethyl-carbazol as chromogen and hematoxylin counterstain; Nomarski contrast).

    Techniques Used: Infection, Immunohistochemistry, Avidin-Biotin Assay

    3) Product Images from "Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets"

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets

    Journal: Journal of Virology

    doi: 10.1128/JVI.01300-17

    (A, B) C11-infected ferret lung at 4 dpi. (A) Mild, acute, catarrhal bronchiolitis with protein-rich fluid, desquamated and degenerating epithelia (arrow), and scant neutrophils within the lumen. (B) Focal spot of influenza A virus nucleoprotein-immunoreactive cellular debris, epithelial cells, and alveolar macrophages interpreted as remnants of a necrotic bronchiolus. (C, D) AR236-infected ferret lung at 4 dpi. (C) Moderate, acute, catarrhal and suppurative (broncho-)pneumonia with intra-alveolar neutrophils (arrow), macrophages (arrowhead), and protein-rich edema. (D) Multifocal accumulation of influenza A virus nucleoprotein-immunoreactive cellular debris, alveolar macrophages, and/or type II pneumocytes. (E, F) C46-infected ferret nasal conchae at 4 dpi. (E) Mild, acute, focal degeneration of epithelial cells (arrow) within the respiratory mucosa. (F) Multiple influenza A virus nucleoprotein-immunoreactive epithelial cells within superficial and deeper layers of the respiratory mucosa. (G, H) C46-infected ferret lung at 4 dpi. (G) Moderate, subacute, coalescing, proliferative pneumonia with hyperplastic type II pneumocytes (arrow) and alveolar histiocytosis. (H) A discrete, large round cell with an influenza A virus nucleoprotein-immunoreactive nucleus and cytoplasm interpreted to be an alveolar macrophage or desquamated type II pneumocyte. (A, C, E, G) Hematoxylin and eosin stain. (B, D, F, G) Influenza A virus nucleoprotein immunohistochemistry by the avidin-biotin-peroxidase complex method with 3-amino-9-ethylcarbazol as the chromogen and hematoxylin counterstain. Bars = 20 μm.
    Figure Legend Snippet: (A, B) C11-infected ferret lung at 4 dpi. (A) Mild, acute, catarrhal bronchiolitis with protein-rich fluid, desquamated and degenerating epithelia (arrow), and scant neutrophils within the lumen. (B) Focal spot of influenza A virus nucleoprotein-immunoreactive cellular debris, epithelial cells, and alveolar macrophages interpreted as remnants of a necrotic bronchiolus. (C, D) AR236-infected ferret lung at 4 dpi. (C) Moderate, acute, catarrhal and suppurative (broncho-)pneumonia with intra-alveolar neutrophils (arrow), macrophages (arrowhead), and protein-rich edema. (D) Multifocal accumulation of influenza A virus nucleoprotein-immunoreactive cellular debris, alveolar macrophages, and/or type II pneumocytes. (E, F) C46-infected ferret nasal conchae at 4 dpi. (E) Mild, acute, focal degeneration of epithelial cells (arrow) within the respiratory mucosa. (F) Multiple influenza A virus nucleoprotein-immunoreactive epithelial cells within superficial and deeper layers of the respiratory mucosa. (G, H) C46-infected ferret lung at 4 dpi. (G) Moderate, subacute, coalescing, proliferative pneumonia with hyperplastic type II pneumocytes (arrow) and alveolar histiocytosis. (H) A discrete, large round cell with an influenza A virus nucleoprotein-immunoreactive nucleus and cytoplasm interpreted to be an alveolar macrophage or desquamated type II pneumocyte. (A, C, E, G) Hematoxylin and eosin stain. (B, D, F, G) Influenza A virus nucleoprotein immunohistochemistry by the avidin-biotin-peroxidase complex method with 3-amino-9-ethylcarbazol as the chromogen and hematoxylin counterstain. Bars = 20 μm.

    Techniques Used: Infection, H&E Stain, Immunohistochemistry, Avidin-Biotin Assay

    4) Product Images from "Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards"

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards

    Journal: Emerging Microbes & Infections

    doi: 10.1080/22221751.2020.1713706

    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.
    Figure Legend Snippet: Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.

    Techniques Used: Infection, Lysis, Immunohistochemistry, Avidin-Biotin Assay

    5) Product Images from "Common Hepatic Branch of Vagus Nerve-Dependent Expression of Immediate Early Genes in the Mouse Brain by Intraportal L-Arginine: Comparison with Cholecystokinin-8"

    Article Title: Common Hepatic Branch of Vagus Nerve-Dependent Expression of Immediate Early Genes in the Mouse Brain by Intraportal L-Arginine: Comparison with Cholecystokinin-8

    Journal: Frontiers in Neuroscience

    doi: 10.3389/fnins.2017.00366

    Immunostaining with anti-c-Fos antibodies of the insular cortex in brain coronal slices from Arc-dVenus mice (the slices used in Figure 2 ). The mice were intraportally injected with saline (A) , Arg ( B , 0.61 mmol/kg), and CCK ( C , 1.75 nmol/kg), and fixed at 180 min after the portal vein injection. The solid black lines indicate the area of the insular cortex, and the dotted lines indicate the border of the granular/dysgranular and agranular insular cortices. The brain slices were treated with anti-c-Fos antibodies and the signals were visualized using the avidin-biotin-peroxidase complex method. Note that c-Fos positive cells (black dots) were increased in the insular cortex of the Arg-injected mouse. Ins: insular cortex. (D) The number of c-Fos positive cells in mice intraportally injected with saline (5 slices from 5 mice), Arg ( B , 0.61 mmol/kg, 5 slices from 5 mice), and CCK ( C , 1.75 nmol/kg, 4 slices from 4 mice) at bregma levels of −0.34 and −1.06 mm. ( D inset) The data from saline-injected (3 slices from 3 mice) and CCK-injected (1.75 nmol/kg, 5 slices from 5 mice) mice. These mice were fixed at 90 min after portal vein injection. Data are shown as mean ± SEM. * P
    Figure Legend Snippet: Immunostaining with anti-c-Fos antibodies of the insular cortex in brain coronal slices from Arc-dVenus mice (the slices used in Figure 2 ). The mice were intraportally injected with saline (A) , Arg ( B , 0.61 mmol/kg), and CCK ( C , 1.75 nmol/kg), and fixed at 180 min after the portal vein injection. The solid black lines indicate the area of the insular cortex, and the dotted lines indicate the border of the granular/dysgranular and agranular insular cortices. The brain slices were treated with anti-c-Fos antibodies and the signals were visualized using the avidin-biotin-peroxidase complex method. Note that c-Fos positive cells (black dots) were increased in the insular cortex of the Arg-injected mouse. Ins: insular cortex. (D) The number of c-Fos positive cells in mice intraportally injected with saline (5 slices from 5 mice), Arg ( B , 0.61 mmol/kg, 5 slices from 5 mice), and CCK ( C , 1.75 nmol/kg, 4 slices from 4 mice) at bregma levels of −0.34 and −1.06 mm. ( D inset) The data from saline-injected (3 slices from 3 mice) and CCK-injected (1.75 nmol/kg, 5 slices from 5 mice) mice. These mice were fixed at 90 min after portal vein injection. Data are shown as mean ± SEM. * P

    Techniques Used: Immunostaining, Mouse Assay, Injection, Avidin-Biotin Assay

    6) Product Images from "Two monoclonal antibodies against glycoprotein Gn protect mice from Rift Valley Fever challenge by cooperative effects"

    Article Title: Two monoclonal antibodies against glycoprotein Gn protect mice from Rift Valley Fever challenge by cooperative effects

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0008143

    Histopathological and immunohistochemical findings in different tissues of three individual mice. Light microscopy revealed three principally different patterns of lesions and antigen distribution in the livers (first row, A-F), brains (second row, G-L), spleens (third row, M-R), and lungs (fourth row, S-X) of mice which succumbed due to the infection within the first 6 days (left column: P18-865, mouse #25, 3 dpi, A, B, G, H, M, N, S, T) later in the time course of the disease (middle column: P18-870, mouse #30, 8 dpi, C, D, I, J, O, P, U, V) and those which survived until the end of the observation period (right column, P18-878, mouse #78, 13 dpi, E, F, K, L, W, X and P18-851, mouse #68, 13 dpi Q,R). A.) The mice dying until 6 dpi display mainly a severe, acute, diffuse, necrotizing hepatitis with Councilman corpuscles (arrows), suggestive of hepatocellular apoptosis. B.) These mice show coalescing to diffuse antigen within hepatocytes. M.) There are few apoptotic or necrotic lymphocytes in the white matter of the spleen (arrow). C.) The few mice dying later than 6 dpi display multifocal infiltrations of macrophages and lymphocytes (arrow) and hepatocellular vacuolar degeneration within the livers. D.) There is no antigen present within the liver. I.) There are oligofocal necrotic neurons and glia (arrows) within the brain, interpreted as necrotizing polioencephalitis. J.) There is multifocal, neuronal antigen accumulation. O.) There are many apoptotic or necrotic lymphocytes (arrow) leading to lymphatic depletion within the spleen. Q.) There is follicular hyperplasia with enlarged follicles with a pale lymphoblast-rich center and a darker peripheral zone of more differentiated lymphocytes. A, C, E, G, I, K, M, O, Q, S, U, W.) hematoxylin-eosin. B, D, F, H, J, L, N, P, R, T, V, X.) Immunohistochemistry employing the avidin-biotin-peroxidase-complex method for RVFV nucleoprotein with AEC as chromogen (red-brown) and hematoxylin counterstain (blue). A-X.) Bars = 50 μm.
    Figure Legend Snippet: Histopathological and immunohistochemical findings in different tissues of three individual mice. Light microscopy revealed three principally different patterns of lesions and antigen distribution in the livers (first row, A-F), brains (second row, G-L), spleens (third row, M-R), and lungs (fourth row, S-X) of mice which succumbed due to the infection within the first 6 days (left column: P18-865, mouse #25, 3 dpi, A, B, G, H, M, N, S, T) later in the time course of the disease (middle column: P18-870, mouse #30, 8 dpi, C, D, I, J, O, P, U, V) and those which survived until the end of the observation period (right column, P18-878, mouse #78, 13 dpi, E, F, K, L, W, X and P18-851, mouse #68, 13 dpi Q,R). A.) The mice dying until 6 dpi display mainly a severe, acute, diffuse, necrotizing hepatitis with Councilman corpuscles (arrows), suggestive of hepatocellular apoptosis. B.) These mice show coalescing to diffuse antigen within hepatocytes. M.) There are few apoptotic or necrotic lymphocytes in the white matter of the spleen (arrow). C.) The few mice dying later than 6 dpi display multifocal infiltrations of macrophages and lymphocytes (arrow) and hepatocellular vacuolar degeneration within the livers. D.) There is no antigen present within the liver. I.) There are oligofocal necrotic neurons and glia (arrows) within the brain, interpreted as necrotizing polioencephalitis. J.) There is multifocal, neuronal antigen accumulation. O.) There are many apoptotic or necrotic lymphocytes (arrow) leading to lymphatic depletion within the spleen. Q.) There is follicular hyperplasia with enlarged follicles with a pale lymphoblast-rich center and a darker peripheral zone of more differentiated lymphocytes. A, C, E, G, I, K, M, O, Q, S, U, W.) hematoxylin-eosin. B, D, F, H, J, L, N, P, R, T, V, X.) Immunohistochemistry employing the avidin-biotin-peroxidase-complex method for RVFV nucleoprotein with AEC as chromogen (red-brown) and hematoxylin counterstain (blue). A-X.) Bars = 50 μm.

    Techniques Used: Immunohistochemistry, Mouse Assay, Light Microscopy, Infection, Avidin-Biotin Assay

    7) Product Images from "Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats"

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    Journal: Viruses

    doi: 10.3390/v10120681

    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Figure Legend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Techniques Used: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets
    Article Snippet: Tissues were fixed in 10% neutral buffered formalin for 21 days, processed, and embedded in paraffin wax using a Leica ASP 300S fully enclosed tissue processor (Leica Biosystems, Nussloch, Germany), sectioned to a thickness of 2 to 4 μm using a Hyrax M55 electronic rotary microtome (Carl Zeiss Microimaging GmbH, Jena, Germany), mounted on Superfrost Plus glass slides (Menzel Gläser, Braunschweig, Germany), stained with hematoxylin and eosin using a Medite TST 44.000C automatic tissue stainer (Medite, Burgdorf, Germany), and screened for histopathological changes using an Axio Imager M2 microscope equipped with 10×, 20×, and 40× Plan Neofluar objectives and an AxioCam ICc3 3.3-megapixel digital camera (Carl Zeiss Microscopy GmbH, Jena, Germany). .. Immunohistochemistry was performed on serial sections to detect influenza A virus antigen using the avidin-biotin-peroxidase complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a polyclonal rabbit anti-influenza A virus FPV/Rostock/34 nucleoprotein antiserum (diluted 1:750) , 3-amino-9-ethylcarbazol as the chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain. .. The specificity of the immunohistochemical reaction was confirmed by the use of negative tissues from noninfected chicken and ferret archival diagnostic cases, as well as validated positive avian tissues from the recent H5N8 outbreak ( ).

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: Hematoxylin and eosin stained sections were evaluated for histopathological lesions using a light microscope, and the severity of parenchymal necrotizing inflammation, as well as lymphatic necrosis, apoptosis and/or depletion in the lymphatic organs was scored on an ordinal 4-step scale (0 = unchanged, 1 = mild, 2 = moderate, 3 = severe). .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Avidin-Biotin Assay:

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets
    Article Snippet: Tissues were fixed in 10% neutral buffered formalin for 21 days, processed, and embedded in paraffin wax using a Leica ASP 300S fully enclosed tissue processor (Leica Biosystems, Nussloch, Germany), sectioned to a thickness of 2 to 4 μm using a Hyrax M55 electronic rotary microtome (Carl Zeiss Microimaging GmbH, Jena, Germany), mounted on Superfrost Plus glass slides (Menzel Gläser, Braunschweig, Germany), stained with hematoxylin and eosin using a Medite TST 44.000C automatic tissue stainer (Medite, Burgdorf, Germany), and screened for histopathological changes using an Axio Imager M2 microscope equipped with 10×, 20×, and 40× Plan Neofluar objectives and an AxioCam ICc3 3.3-megapixel digital camera (Carl Zeiss Microscopy GmbH, Jena, Germany). .. Immunohistochemistry was performed on serial sections to detect influenza A virus antigen using the avidin-biotin-peroxidase complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a polyclonal rabbit anti-influenza A virus FPV/Rostock/34 nucleoprotein antiserum (diluted 1:750) , 3-amino-9-ethylcarbazol as the chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain. .. The specificity of the immunohistochemical reaction was confirmed by the use of negative tissues from noninfected chicken and ferret archival diagnostic cases, as well as validated positive avian tissues from the recent H5N8 outbreak ( ).

    Article Title: Title: Multi-Scale LM/EM Neuronal Imaging from Brain to Synapse with a Tissue Clearing Method, ScaleSF
    Article Snippet: Some of the sections were counterstained with DAPI (1 μg/ml, D1306, Thermo Fisher Scientific) in PBS for 2 hr on ice. .. The sections were then reacted with avidin-biotinylated peroxidase complex (ABC) (1:50 diluted in PBS, PK-6100, Vector Laboratories) in PBS containing 2% BSA overnight at 4°C and developed in DAB-Ni2+ solution on ice. .. CLSM imaging was performed prior to the ABC reaction.

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards
    Article Snippet: Hematoxylin and eosin stained sections were evaluated for histopathological lesions using a light microscope, and the severity of parenchymal necrotizing inflammation, as well as lymphatic necrosis, apoptosis and/or depletion in the lymphatic organs was scored on an ordinal 4-step scale (0 = unchanged, 1 = mild, 2 = moderate, 3 = severe). .. Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ]. ..

    Article Title: Two monoclonal antibodies against glycoprotein Gn protect mice from Rift Valley Fever challenge by cooperative effects
    Article Snippet: .. Immunohistology was performed with the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain. .. The primary antibody used for the detection of RVFV nucleoprotein was a heat-inactivated serum of a sheep immunized with recombinant RVFV MP12-strain nucleoprotein (internal code: S24NP) in a dilution of 1:4000.

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats
    Article Snippet: Tissue specimens were fixed in 4% neutral buffered formaldehyde, processed, embedded in paraffin wax, sectioned at 2–4 μm thickness, and stained with hematoxylin and eosin. .. Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain. ..

    Immunoperoxidase Staining:

    Article Title: Matrix Gla Protein Binds to Fibronectin and Enhances Cell Attachment and Spreading on Fibronectin
    Article Snippet: .. Immunoperoxidase staining followed the Vectastain ABC Elite kit instructions (Vector labs). .. Sections were blocked with buffer containing normal horse serum at 1.5% then incubated with rabbit anti-MGP at 20 μ g/mL or mouse antifibronectin monoclonal antibody (clone IST-3 Sigma) at 1 : 5000 dilution.

    Incubation:

    Article Title: Early life trauma increases threat response of peri-weaning rats, reduction of axo-somatic synapses formed by parvalbumin cells and perineuronal net in the basolateral nucleus of amygdala
    Article Snippet: After a ~48 hr incubation at room temperature with the mouse anti-PV antibody diluted in PBS-BSA-Azide (1:10,000), tissue was washed with PBS and incubated for 1 hr in biotinylated goat-anti-mouse IgG secondary antibody (Vector laboratories, Burlingame, CA, cat# BA-9200, dilution 1:200). .. Tissue was then washed in 0.01M PBS, incubated in a solution of the Vectastain Elite ABC HRP kit (Vector Laboratories, Burlingame, CA, cat# PK-6100), washed with 0.01M PBS, and incubated for 9 min in a filtered solution of 3’3-diaminobenzidine tetrahydrochloride (DAB; 10mg tablet Sigma Aldrich in 44ml of PBS buffer), catalyzed by 0.003% hydrogen peroxide. ..

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    Vector Laboratories avidin biotin peroxidase complex method
    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, <t>avidin-biotin-peroxidase-complex</t> <t>method,</t> 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.
    Avidin Biotin Peroxidase Complex Method, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Journal: Viruses

    Article Title: Productive Propagation of Rift Valley Fever Phlebovirus Vaccine Strain MP-12 in Rousettus aegyptiacus Fruit Bats

    doi: 10.3390/v10120681

    Figure Lengend Snippet: Histopathological findings in the liver of a Rousettus aegyptiacus fruit bat immunized with the MP-12 vaccine strain at day seven post immunization. ( A ) Histopathology shows few, randomly distributed foci of hepatocellular necrosis and loss with macrophage and lymphocyte infiltration (arrow). Furthermore, the hepatocytes display moderate, coalescing to diffuse, floccular cytoplasmic vacuolization, interpreted as a species-specific, relatively high level of glycogen storage. Hematoxylin-eosin. Bar = 100 μm; ( B ) Immunohistochemistry for Rift Valley fever phlebovirus (RVFV) Gc antigen reveals minor amounts of intra- and extracellular, strongly immunoreactive granula within the lesions (arrow), interpreted as debris remaining after virus-induced hepatocellular death. Immunohistochemistry, monoclonal mouse anti-RVFV Gc-protein antibody, avidin-biotin-peroxidase-complex method, 3-amino-9-ethyl-carbazol chromogen (red), hematoxylin counterstain (blue). Bar = 20 μm.

    Article Snippet: Immunohistology was performed using a mouse monoclonal antibody against the RVFV Gc-protein (clone: GC9A9) [ ], the avidin–biotin–peroxidase complex method (ABC, Elite PK6100; Vector Laboratories, Burlingame, CA, USA) with 3-amino-9-ethylcarbazole (AEC, Dako, Glostrup, Denmark) as chromogen and hematoxylin counterstain.

    Techniques: Histopathology, Immunohistochemistry, Avidin-Biotin Assay

    Virus score: Immunohistological scoring of influenza A virus (IAV)-antigen distribution, avian influenza virus (AIV)-infected birds (bar = 100 µm, IAV-matrixprotein immunohistochemistry, avidin-biotin-peroxidase complex method, 3-amino-9-ethyl-carbazol as chromogen and hematoxylin counterstain; Nomarski contrast).

    Journal: Viruses

    Article Title: A Semiquantitative Scoring System for Histopathological and Immunohistochemical Assessment of Lesions and Tissue Tropism in Avian Influenza

    doi: 10.3390/v13050868

    Figure Lengend Snippet: Virus score: Immunohistological scoring of influenza A virus (IAV)-antigen distribution, avian influenza virus (AIV)-infected birds (bar = 100 µm, IAV-matrixprotein immunohistochemistry, avidin-biotin-peroxidase complex method, 3-amino-9-ethyl-carbazol as chromogen and hematoxylin counterstain; Nomarski contrast).

    Article Snippet: Immunohistochemical examination was performed on formaldehyde-fixed and paraffin-embedded (FFPE) tissue samples with the avidin-biotin-peroxidase complex method (Vector Laboratories, Burlingame, CA, USA) using a polyclonal rabbit anti-influenza A-nucleoprotein (NP) antibody [ , , ] or monoclonal murine anti-influenza A-matrixprotein (MP) antibody [ ] with 3-amino-9-ethyl-carbazol as chromogen and hematoxylin counterstain in individual experiments (see ).

    Techniques: Infection, Immunohistochemistry, Avidin-Biotin Assay

    (A, B) C11-infected ferret lung at 4 dpi. (A) Mild, acute, catarrhal bronchiolitis with protein-rich fluid, desquamated and degenerating epithelia (arrow), and scant neutrophils within the lumen. (B) Focal spot of influenza A virus nucleoprotein-immunoreactive cellular debris, epithelial cells, and alveolar macrophages interpreted as remnants of a necrotic bronchiolus. (C, D) AR236-infected ferret lung at 4 dpi. (C) Moderate, acute, catarrhal and suppurative (broncho-)pneumonia with intra-alveolar neutrophils (arrow), macrophages (arrowhead), and protein-rich edema. (D) Multifocal accumulation of influenza A virus nucleoprotein-immunoreactive cellular debris, alveolar macrophages, and/or type II pneumocytes. (E, F) C46-infected ferret nasal conchae at 4 dpi. (E) Mild, acute, focal degeneration of epithelial cells (arrow) within the respiratory mucosa. (F) Multiple influenza A virus nucleoprotein-immunoreactive epithelial cells within superficial and deeper layers of the respiratory mucosa. (G, H) C46-infected ferret lung at 4 dpi. (G) Moderate, subacute, coalescing, proliferative pneumonia with hyperplastic type II pneumocytes (arrow) and alveolar histiocytosis. (H) A discrete, large round cell with an influenza A virus nucleoprotein-immunoreactive nucleus and cytoplasm interpreted to be an alveolar macrophage or desquamated type II pneumocyte. (A, C, E, G) Hematoxylin and eosin stain. (B, D, F, G) Influenza A virus nucleoprotein immunohistochemistry by the avidin-biotin-peroxidase complex method with 3-amino-9-ethylcarbazol as the chromogen and hematoxylin counterstain. Bars = 20 μm.

    Journal: Journal of Virology

    Article Title: Natural Reassortants of Potentially Zoonotic Avian Influenza Viruses H5N1 and H9N2 from Egypt Display Distinct Pathogenic Phenotypes in Experimentally Infected Chickens and Ferrets

    doi: 10.1128/JVI.01300-17

    Figure Lengend Snippet: (A, B) C11-infected ferret lung at 4 dpi. (A) Mild, acute, catarrhal bronchiolitis with protein-rich fluid, desquamated and degenerating epithelia (arrow), and scant neutrophils within the lumen. (B) Focal spot of influenza A virus nucleoprotein-immunoreactive cellular debris, epithelial cells, and alveolar macrophages interpreted as remnants of a necrotic bronchiolus. (C, D) AR236-infected ferret lung at 4 dpi. (C) Moderate, acute, catarrhal and suppurative (broncho-)pneumonia with intra-alveolar neutrophils (arrow), macrophages (arrowhead), and protein-rich edema. (D) Multifocal accumulation of influenza A virus nucleoprotein-immunoreactive cellular debris, alveolar macrophages, and/or type II pneumocytes. (E, F) C46-infected ferret nasal conchae at 4 dpi. (E) Mild, acute, focal degeneration of epithelial cells (arrow) within the respiratory mucosa. (F) Multiple influenza A virus nucleoprotein-immunoreactive epithelial cells within superficial and deeper layers of the respiratory mucosa. (G, H) C46-infected ferret lung at 4 dpi. (G) Moderate, subacute, coalescing, proliferative pneumonia with hyperplastic type II pneumocytes (arrow) and alveolar histiocytosis. (H) A discrete, large round cell with an influenza A virus nucleoprotein-immunoreactive nucleus and cytoplasm interpreted to be an alveolar macrophage or desquamated type II pneumocyte. (A, C, E, G) Hematoxylin and eosin stain. (B, D, F, G) Influenza A virus nucleoprotein immunohistochemistry by the avidin-biotin-peroxidase complex method with 3-amino-9-ethylcarbazol as the chromogen and hematoxylin counterstain. Bars = 20 μm.

    Article Snippet: Immunohistochemistry was performed on serial sections to detect influenza A virus antigen using the avidin-biotin-peroxidase complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a polyclonal rabbit anti-influenza A virus FPV/Rostock/34 nucleoprotein antiserum (diluted 1:750) , 3-amino-9-ethylcarbazol as the chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain.

    Techniques: Infection, H&E Stain, Immunohistochemistry, Avidin-Biotin Assay

    Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.

    Journal: Emerging Microbes & Infections

    Article Title: Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards

    doi: 10.1080/22221751.2020.1713706

    Figure Lengend Snippet: Light microscopic finding in the livers of experimentally H5N8B-infected ducks. (A, B) Seropositive mallard, H5N8B-infected, clinically normal, 34 dpi, liver. (A) No obvious findings. (B) Lack of immunohistochemically-detectable hepatocellular influenza A virus matrixprotein antigen. (C, D) Pekin duck, contact animal, died 4 days post contact, liver. (C) Marked hypereosinophilia, hepatocellular vacuolation, membraneous rupture and nuclear pyknosis, karyorrhexis and lysis interpreted as severe, acute, coalescing to diffuse necrotizing hepatitis. (D) Immunohistochemistry reveals coalescing intrahepatocytic, intracytoplasmic and intranuclear influenza A virus matrix protein. (A, C) Hematoxylin-eosin, (B, D) Immunohistochemistry using the avidin-biotin-peroxidase-complex method with a monoclonal antibody against influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (redbrown) and hematoxylin counterstain (blue). (A, C) bars = 20 μm. (B, D) bars = 50 μm.

    Article Snippet: Immunohistochemistry was employed to detect influenza A virus matrix protein using the avidin–biotin-peroxidase-complex method (Vectastain PK 6100; Vector Laboratories, Burlingame, CA, USA) with citric buffer (10 mM, pH 6.0) pretreatment, a monoclonal antibody (mAb) directed against an epitope of the influenza A virus matrix protein (ATCC clone HB-64), 3-amino-9-ethylcarbazol chromogen (Agilent Technologies, Santa Clara, CA, USA), and hematoxylin counterstain [ ].

    Techniques: Infection, Lysis, Immunohistochemistry, Avidin-Biotin Assay