avidin biotin complex  (Vector Laboratories)


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    Name:
    Avidin D Alkaline Phosphatase labeled
    Description:
    Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
    Catalog Number:
    a-2100
    Price:
    None
    Category:
    Proteins
    Size:
    100U
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin complex
    Avidin D Alkaline Phosphatase labeled
    Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
    https://www.bioz.com/result/avidin biotin complex/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Expression of ?F508 Cystic Fibrosis Transmembrane Regulator (CFTR) Decreases Membrane Sialylation
    Article Snippet: We used a combination of ELISA, immunocytochemistry and FACS to demonstrate the differential presence of sialic acid residues recognized by specific lectins and quantitative sialic acid analysis, and to determine whether the ΔF508 mutation of the CFTR is responsible for the sialylation defects noted in this common form of cystic fibrosis. .. Reagents Fluorescein and Biotin conjugates of Maackia amaurensis lectin (MAL II), avidin D-alkaline phosphatase, Vector Laboratories (Burlingame, CA); biotinlylated conjugate of cholera toxin, List Biological Lab, Campbell, CA. .. ; N-acetylneuraminic acid and N-glycolylneuraminic acid1,2 diamino-4,5-methylenedioxybenzene, N-propyl gallate, N-ethylmaleimide, PMSF, leupeptin, TBST, goat anti-rabbit alkaline phosphatase, Sigma Chemical Company, St. Louis, MO; Biorad protein standard assay, alkaline phosphatase substrate kit (p-nitrophenylphosphate, diethanolamine buffer), BioRad, Hercules, CA.

    Article Title: Somatostatin receptor 1–5; expression profiles during rat development
    Article Snippet: .. Briefly, the immune reaction was amplified by an avidin-biotin complex coupled to alkaline phosphates (Vectastain ABC-AP; Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Vector Red (Vector Laboratories) as substrate ( ). ..

    Article Title: Angiotensin I-Converting Enzyme Type 2 (ACE2) Gene Therapy Improves Glycemic Control in Diabetic Mice
    Article Snippet: Sections were incubated with an anti-insulin primary antibody (1:100; Abcam, Cambridge, MA) for 4 h at 4°C, followed by incubation with biotinylated anti–guinea pig secondary antibody (1:200; Vector Laboratories, Burlingame, CA) for 1 h at room temperature. .. Sections were then treated with avidin-biotin complex reagent and developed using alkaline phosphatase red according to the manufacturer's protocol (Vector Laboratories). .. Pancreatic islet insulin content was calculated as the total insulin staining per islet area.

    Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis
    Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of avidin-biotin alkaline phosphatase complex (Vector Laboratories, Burlingame, Calif.) diluted 1:100 in PBS was added for 45 min. Unbound avidin-biotin alkaline phosphatase complex was removed by five consecutive washings with Tween-PBS, and 100 μl of freshly prepared enzyme substrate solution was added to each well. .. Absorbance was measured after 15 min of incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: p27Kip1 and p57Kip2 Regulate Proliferation in Distinct Retinal Progenitor Cell Populations
    Article Snippet: The secondary biotinylated antibody (donkey anti-mouse IgG; Vector Laboratories) was used at a dilution of 1:2000. .. Amplification was achieved by incubating the immunoblot with an avidin–biotin–alkaline phosphatase complex (Vectastain-AP, Vector Laboratories) followed by nitro blue tetrazolium–5-bromo-4-chloro-3-indolyl phosphate detection (Vector Laboratories). ..

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

    Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
    Article Snippet: Wildtype chicken avidin (Belovo, Belgium, MW = 16 kDa/monomer) and fibronectin (gelatin-affinity purified from human serum, MW = 220 kDa/monomer) had been previously labeled with Alexa Fluor® 488 NHS ester (Cat. No. A-20000, Thermo Fisher Scientific, Inc. Waltham, MA, USA) by Dr. J. Pärssinen according to manufacturer’s instructions. .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and biotinylated alkaline phosphatase (Cat. No. B-2005, Vector Laboratories (Burlingame, CA, USA) were used. .. Vectashield Hardset Antifade Mounting Medium (Cat. No. H-1400, Vector Laboratories) was used for microscope sample preparation.

    Article Title: Oxidative Stress Induced Age Dependent Meibomian Gland Dysfunction in Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice
    Article Snippet: Tissue samples were washed with PBS and then 3.0% H2O2 in methanol was applied for 3 minutes to block endogenous peroxidase activity. .. The sections were incubated for 30 minutes with biotin-labeled horse anti-mouse IgG serum (Vector Laboratories, Burlingame, CA), followed by treatment with avidin-biotin-alkaline phosphatase complex (Vector Laboratories, Burlingame, CA) for 30 minutes. .. All sections were washed in PBS buffer, developed in 3,3′-diaminobenzidine (DAB) chromogen solution (Vector Laboratories, Burlingame, CA).

    Amplification:

    Article Title: Somatostatin receptor 1–5; expression profiles during rat development
    Article Snippet: .. Briefly, the immune reaction was amplified by an avidin-biotin complex coupled to alkaline phosphates (Vectastain ABC-AP; Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Vector Red (Vector Laboratories) as substrate ( ). ..

    Article Title: p27Kip1 and p57Kip2 Regulate Proliferation in Distinct Retinal Progenitor Cell Populations
    Article Snippet: The secondary biotinylated antibody (donkey anti-mouse IgG; Vector Laboratories) was used at a dilution of 1:2000. .. Amplification was achieved by incubating the immunoblot with an avidin–biotin–alkaline phosphatase complex (Vectastain-AP, Vector Laboratories) followed by nitro blue tetrazolium–5-bromo-4-chloro-3-indolyl phosphate detection (Vector Laboratories). ..

    Plasmid Preparation:

    Article Title: Somatostatin receptor 1–5; expression profiles during rat development
    Article Snippet: .. Briefly, the immune reaction was amplified by an avidin-biotin complex coupled to alkaline phosphates (Vectastain ABC-AP; Vector Laboratories Inc., Burlingame, CA, USA) and visualized with Vector Red (Vector Laboratories) as substrate ( ). ..

    Incubation:

    Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis
    Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of avidin-biotin alkaline phosphatase complex (Vector Laboratories, Burlingame, Calif.) diluted 1:100 in PBS was added for 45 min. Unbound avidin-biotin alkaline phosphatase complex was removed by five consecutive washings with Tween-PBS, and 100 μl of freshly prepared enzyme substrate solution was added to each well. .. Absorbance was measured after 15 min of incubation in the dark in a 405 Multiscan photometer (mcc/340; Labsystem, Helsinki, Finland).

    Article Title: Oxidative Stress Induced Age Dependent Meibomian Gland Dysfunction in Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice
    Article Snippet: Tissue samples were washed with PBS and then 3.0% H2O2 in methanol was applied for 3 minutes to block endogenous peroxidase activity. .. The sections were incubated for 30 minutes with biotin-labeled horse anti-mouse IgG serum (Vector Laboratories, Burlingame, CA), followed by treatment with avidin-biotin-alkaline phosphatase complex (Vector Laboratories, Burlingame, CA) for 30 minutes. .. All sections were washed in PBS buffer, developed in 3,3′-diaminobenzidine (DAB) chromogen solution (Vector Laboratories, Burlingame, CA).

    Transduction:

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

    Staining:

    Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection
    Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and avidin–biotin complex–alkaline phosphatase (ABC–AP; Vector, Burlingame, CA). .. Production of IL-10 by the transduced DC was determined by enzyme-linked immunosorbent assay (ELISA) measurement of mouse IL-10 in the supernatants of 106 DC cultured for 24-hr periods from 0, 24 and 48 hr after gene transfer.

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    Vector Laboratories avidin biotin peroxidase complex abc
    Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the <t>ABC</t> method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a <t>chlamydial</t> inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).
    Avidin Biotin Peroxidase Complex Abc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    99
    Vector Laboratories avidin biotin complex horseradish peroxidase immunostain techniques
    Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of <t>immunostain</t> intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]
    Avidin Biotin Complex Horseradish Peroxidase Immunostain Techniques, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex horseradish peroxidase immunostain techniques/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
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    99
    Vector Laboratories avidin biotin immunoperoxidase technique
    Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin <t>immunoperoxidase</t>
    Avidin Biotin Immunoperoxidase Technique, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin immunoperoxidase technique/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a chlamydial inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).

    Journal: Infection and Immunity

    Article Title: Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion

    doi:

    Figure Lengend Snippet: Histopathology and immunoreaction in the placentas of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions (arrowheads and arrows) and areas of necrosis (asterisks) in the placentas of depleted (a and c) and nondepleted (b and d) mice at 5 days p.i. (a) Placenta of a depleted mouse showing only one focus of immunoreaction but extensive areas of necrosis in the decidua basalis and labyrinth; (b) placenta of a nondepleted mouse showing immunoreactive foci spread through the metrial gland and decidua basalis; (c) area of necrosis in the labyrinth of a depleted mouse showing a chlamydial inclusion (arrow) (notice the pyknosis of the nuclei in laberynthine cells); (d) substantial neutrophil infiltration in the decidua basalis of a nondepleted mouse. Magnifications, ×40 (a and b) and ×200 (c and d).

    Article Snippet: To visualize chlamydial antigen in paraffin sections, immunohistochemical staining was carried out with a chlamydial-lipopolysaccharide-specific biotinylated mouse MAb, as previously described , by using the avidin-biotin-peroxidase complex (ABC) method according to the instructions of the manufacturer (Vector Laboratories, Burlingame, Calif.).

    Techniques: Histopathology, Mouse Assay, Infection

    Histopathology and immunoreaction in the livers of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions in the livers of depleted (a and c) and nondepleted (b and d) pregnant mice. (a) Liver of a depleted mouse at 3 days p.i. showing several chlamydial inclusions (notice the lack of leukocytes); (b) liver of a nondepleted mouse at 3 days p.i. showing an infectious focus, formed mainly by neutrophils, with a positive immunoreaction (notice that there are not typical chlamydial inclusions); (c) liver of a depleted mouse at 7 days p.i. showing numerous immunoreactive foci; (d) liver of a nondepleted mouse at 7 days p.i. showing extensive leukocyte infiltration (notice the lack of immunoreactivity in the foci). Magnifications, ×200 (a and b) and ×100 (c and d).

    Journal: Infection and Immunity

    Article Title: Role of Polymorphonuclear Neutrophils in a Murine Model of Chlamydia psittaci-Induced Abortion

    doi:

    Figure Lengend Snippet: Histopathology and immunoreaction in the livers of mice infected with C. psittaci . Paraffin wax sections immunostained by the ABC method show the distribution of positive immunoreactions in the livers of depleted (a and c) and nondepleted (b and d) pregnant mice. (a) Liver of a depleted mouse at 3 days p.i. showing several chlamydial inclusions (notice the lack of leukocytes); (b) liver of a nondepleted mouse at 3 days p.i. showing an infectious focus, formed mainly by neutrophils, with a positive immunoreaction (notice that there are not typical chlamydial inclusions); (c) liver of a depleted mouse at 7 days p.i. showing numerous immunoreactive foci; (d) liver of a nondepleted mouse at 7 days p.i. showing extensive leukocyte infiltration (notice the lack of immunoreactivity in the foci). Magnifications, ×200 (a and b) and ×100 (c and d).

    Article Snippet: To visualize chlamydial antigen in paraffin sections, immunohistochemical staining was carried out with a chlamydial-lipopolysaccharide-specific biotinylated mouse MAb, as previously described , by using the avidin-biotin-peroxidase complex (ABC) method according to the instructions of the manufacturer (Vector Laboratories, Burlingame, Calif.).

    Techniques: Histopathology, Mouse Assay, Infection

    Levels of TMEV in the brain and spinal cord of 3D transgenic mice. (A) Viral transcripts were quantified by real-time RT-PCR by using SYBR green. Primers specific for VP2 were used. Viral transcripts calculated from nonspecific amplification in uninfected FVB mice were divided by the viral transcripts of infected mice to set the baseline sensitivity of the assay to 1. White bars show values of infected nontransgenic mice, and dark bars show values of 3D transgenic mice. Data are shown as means ± SEM of five mice per group. WT, wild type. (B) Percentages of spinal cord quadrants containing TMEV-positive cells in the white matter are shown after 45 and 270 dpi by the same immunoperoxidase staining technique as that used for the brain. Each point represents one mouse. (C) Correlation of PFU from plaque assay and relative viral transcripts by real-time RT-PCR after the infection of SQSV cells with TMEV for 8, 16, and 24 h. The same VP2-specific primers were used as those for panel A. Each point represents data obtained from separate replicate samples for both assays.

    Journal: Journal of Virology

    Article Title: Transgenic Expression of the 3D Polymerase Inhibits Theiler's Virus Infection and Demyelination ▿Transgenic Expression of the 3D Polymerase Inhibits Theiler's Virus Infection and Demyelination ▿ †

    doi: 10.1128/JVI.00664-09

    Figure Lengend Snippet: Levels of TMEV in the brain and spinal cord of 3D transgenic mice. (A) Viral transcripts were quantified by real-time RT-PCR by using SYBR green. Primers specific for VP2 were used. Viral transcripts calculated from nonspecific amplification in uninfected FVB mice were divided by the viral transcripts of infected mice to set the baseline sensitivity of the assay to 1. White bars show values of infected nontransgenic mice, and dark bars show values of 3D transgenic mice. Data are shown as means ± SEM of five mice per group. WT, wild type. (B) Percentages of spinal cord quadrants containing TMEV-positive cells in the white matter are shown after 45 and 270 dpi by the same immunoperoxidase staining technique as that used for the brain. Each point represents one mouse. (C) Correlation of PFU from plaque assay and relative viral transcripts by real-time RT-PCR after the infection of SQSV cells with TMEV for 8, 16, and 24 h. The same VP2-specific primers were used as those for panel A. Each point represents data obtained from separate replicate samples for both assays.

    Article Snippet: Following incubation with a secondary biotinylated antibody (Vector Laboratories, Burlingame, CA), immunoreactivity was detected using the avidin-biotin immunoperoxidase technique (Vector Laboratories).

    Techniques: Transgenic Assay, Mouse Assay, Quantitative RT-PCR, SYBR Green Assay, Amplification, Infection, Immunoperoxidase Staining, Plaque Assay

    Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of immunostain intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]

    Journal: Journal of neuroscience research

    Article Title: Spatially Patterned Gene Expression for Guided Neurite Extension

    doi: 10.1002/jnr.21908

    Figure Lengend Snippet: Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of immunostain intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]

    Article Snippet: At the end of the culture period, HEK293T cells were fixed with 2% paraformaldehyde in PBS for 10 min. HEK293T cells were stained for NGF with anti-NGF-β (Millipore) and avidin–biotin complex–horseradish peroxidase immunostain techniques (Vector Labs; Burlingame, CA).

    Techniques: Immunocytochemistry, Cell Culture, Negative Control

    Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin immunoperoxidase

    Journal:

    Article Title: Increased Epitope-Specific CD8+ T Cells Prevent Murine Coronavirus Spread to the Spinal Cord and Subsequent Demyelination

    doi: 10.1128/JVI.79.6.3370-3381.2005

    Figure Lengend Snippet: Antigen spread in brains of RA59-gfp/gp33-infected mice receiving virus-specific CD8 + T cells. Brain samples from mice were preserved in formalin, embedded in paraffin, and sectioned sagittally. Sections were stained by an avidin-biotin immunoperoxidase

    Article Snippet: Antigen staining was performed by the avidin-biotin-immunoperoxidase technique (Vector Laboratories, Burlingame, Calif.) by using diaminobenzidine tetrahydrochloride as the substrate and a 1:20 dilution of rabbit antinucleocapsid monoclonal antibody (kindly provided by Julian Leibowitz).

    Techniques: Infection, Mouse Assay, Staining, Avidin-Biotin Assay