avidin biotin complex (Vector Laboratories)
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Vector Laboratories manufactures this product 
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Name:
Avidin D Alkaline Phosphatase labeled
Description:
Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
Catalog Number:
a-2100
Price:
None
Category:
Proteins
Size:
100U
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Structured Review
Vector Laboratories
avidin biotin complex
Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
https://www.bioz.com/result/avidin biotin complex/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Alkaline Phosphatase Avidin D reagent is a powerful tool to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications One unit is 1 mole p nitrophenylphosphate hydrolyzed per min at 37 C pH 9 8
https://www.bioz.com/result/avidin biotin complex/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
avidin biotin complex - by Bioz Stars,
2021-03
86/100 stars
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Avidin-Biotin Assay:Article Title: Expression of ?F508 Cystic Fibrosis Transmembrane Regulator (CFTR) Decreases Membrane Sialylation Article Snippet: We used a combination of ELISA, immunocytochemistry and FACS to demonstrate the differential presence of sialic acid residues recognized by specific lectins and quantitative sialic acid analysis, and to determine whether the ΔF508 mutation of the CFTR is responsible for the sialylation defects noted in this common form of cystic fibrosis. .. Reagents Fluorescein and Biotin conjugates of Maackia amaurensis lectin (MAL II), Article Title: Somatostatin receptor 1–5; expression profiles during rat development Article Snippet: .. Briefly, the immune reaction was amplified by an Article Title: Angiotensin I-Converting Enzyme Type 2 (ACE2) Gene Therapy Improves Glycemic Control in Diabetic Mice Article Snippet: Sections were incubated with an anti-insulin primary antibody (1:100; Abcam, Cambridge, MA) for 4 h at 4°C, followed by incubation with biotinylated anti–guinea pig secondary antibody (1:200; Vector Laboratories, Burlingame, CA) for 1 h at room temperature. .. Sections were then treated with Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of Article Title: p27Kip1 and p57Kip2 Regulate Proliferation in Distinct Retinal Progenitor Cell Populations Article Snippet: The secondary biotinylated antibody (donkey anti-mouse IgG; Vector Laboratories) was used at a dilution of 1:2000. .. Amplification was achieved by incubating the immunoblot with an Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and Article Title: Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology Article Snippet: Wildtype chicken avidin (Belovo, Belgium, MW = 16 kDa/monomer) and fibronectin (gelatin-affinity purified from human serum, MW = 220 kDa/monomer) had been previously labeled with Alexa Fluor® 488 NHS ester (Cat. No. A-20000, Thermo Fisher Scientific, Inc. Waltham, MA, USA) by Dr. J. Pärssinen according to manufacturer’s instructions. .. Chemically competent E. coli Top10 cells were acquired from Thermo Fisher scientific, Inc. For biofunctionalization, neutral chimeric avidin (nChiAvd ) and Article Title: Oxidative Stress Induced Age Dependent Meibomian Gland Dysfunction in Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Article Snippet: Tissue samples were washed with PBS and then 3.0% H2O2 in methanol was applied for 3 minutes to block endogenous peroxidase activity. .. The sections were incubated for 30 minutes with biotin-labeled horse anti-mouse IgG serum (Vector Laboratories, Burlingame, CA), followed by treatment with Amplification:Article Title: Somatostatin receptor 1–5; expression profiles during rat development Article Snippet: .. Briefly, the immune reaction was amplified by an Article Title: p27Kip1 and p57Kip2 Regulate Proliferation in Distinct Retinal Progenitor Cell Populations Article Snippet: The secondary biotinylated antibody (donkey anti-mouse IgG; Vector Laboratories) was used at a dilution of 1:2000. .. Amplification was achieved by incubating the immunoblot with an Plasmid Preparation:Article Title: Somatostatin receptor 1–5; expression profiles during rat development Article Snippet: .. Briefly, the immune reaction was amplified by an Incubation:Article Title: A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis Article Snippet: To detect any captured IFN-γ, 100 μl of biotinylated anti-IFN-γ (7B-B6-1) MAb (0.5 μg/ml; Mabtech) diluted in PBS containing 0.5% Tween 20 and 2% bovine serum albumin was added to the wells. .. After another 60 min of incubation at 37°C and five washes, 100 μl of Article Title: Oxidative Stress Induced Age Dependent Meibomian Gland Dysfunction in Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Article Snippet: Tissue samples were washed with PBS and then 3.0% H2O2 in methanol was applied for 3 minutes to block endogenous peroxidase activity. .. The sections were incubated for 30 minutes with biotin-labeled horse anti-mouse IgG serum (Vector Laboratories, Burlingame, CA), followed by treatment with Transduction:Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and Staining:Article Title: Contrasting effects of myeloid dendritic cells transduced with an adenoviral vector encoding interleukin-10 on organ allograft and tumour rejection Article Snippet: Two days later, LacZ expression was quantified by X-gal staining to determine transduction efficiency. .. The efficiency of IL-10 gene transduction was determined on glass slides by immunocytochemical staining for the gene product, using anti-mouse IL-10 monoclonal antibody (mAb; clone JES5–16E3; PharMingen, San Diego, CA), followed by biotinylated polyclonal anti-rat immunoglobulin (1 : 100; PharMingen) and |
![Immunocytochemistry for the detection of surface-associated NGF. The accessory cell culture was confluent before ICC ( a ). The accessory cell culture surface stains positive for NGF at the site of patterned gene delivery for 24 and 48 hr culture, respectively ( b,d ), and 0.5 mm from the center of the pattern for 24 and 48 hr culture, respectively ( c,e ), compared with negative control (no pNGF delivery) ( f ). Quantification of <t>immunostain</t> intensity vs. distance from the pattern center, plotted on a log scale ( g ). Values are reported as mean ± SEM. *Statistically significantly different at P .]](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764322/bin/nihms112400f6.jpg)
