avidin biotin complex horseradish peroxidase solution  (Agilent technologies)

 
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    Structured Review

    Agilent technologies avidin biotin complex horseradish peroxidase solution
    Avidin Biotin Complex Horseradish Peroxidase Solution, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin complex horseradish peroxidase solution/product/Agilent technologies
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    avidin biotin complex horseradish peroxidase solution - by Bioz Stars, 2020-09
    85/100 stars

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    Incubation:

    Article Title: Anti-inflammatory/anti-fibrotic effects of the hepatoprotective silymarin and the schistosomicide praziquantel against Schistosoma mansoni-induced liver fibrosis
    Article Snippet: .. After rinsing in PBS, the sections were incubated at room temperature for 15 minutes with biotinylated secondary anti-mouse antibody and after a further wash in PBS, the slides were incubated with an avidin-biotin complex horseradish peroxidase solution (DAKO, Glostrup, Denmark). .. After 10 minutes of incubation, the peroxidase reaction was developed using 0.01% hydrogen peroxide in 0.05% diaminobenzidine tetrahydrochloride (DAB).

    Article Title: Antischistosomal and anti-inflammatory activity of garlic and allicin compared with that of praziquantel in vivo
    Article Snippet: .. After one rinse in PBS, the slices were incubated at room temperature for 15 min with a biotinylated secondary anti-mouse antibody, washed again in PBS, and incubated with an avidin-biotin complex horseradish peroxidase solution (DAKO, Glostrup, Denmark). .. After 10 min of incubation, the peroxidase reaction was developed using 0.01% hydrogen peroxide in 0.05% diaminobenzidinetetrahydrochloride (DAB).

    Avidin-Biotin Assay:

    Article Title: Anti-inflammatory/anti-fibrotic effects of the hepatoprotective silymarin and the schistosomicide praziquantel against Schistosoma mansoni-induced liver fibrosis
    Article Snippet: .. After rinsing in PBS, the sections were incubated at room temperature for 15 minutes with biotinylated secondary anti-mouse antibody and after a further wash in PBS, the slides were incubated with an avidin-biotin complex horseradish peroxidase solution (DAKO, Glostrup, Denmark). .. After 10 minutes of incubation, the peroxidase reaction was developed using 0.01% hydrogen peroxide in 0.05% diaminobenzidine tetrahydrochloride (DAB).

    Article Title: Antischistosomal and anti-inflammatory activity of garlic and allicin compared with that of praziquantel in vivo
    Article Snippet: .. After one rinse in PBS, the slices were incubated at room temperature for 15 min with a biotinylated secondary anti-mouse antibody, washed again in PBS, and incubated with an avidin-biotin complex horseradish peroxidase solution (DAKO, Glostrup, Denmark). .. After 10 min of incubation, the peroxidase reaction was developed using 0.01% hydrogen peroxide in 0.05% diaminobenzidinetetrahydrochloride (DAB).

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    Agilent technologies horseradish peroxidase hrp conjugated mouse anti goat antibodies
    ELISA competitive experiments showing that CIP inhibits the formation of the C3d–CR2/CD21 complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by <t>anti-C3</t> pAb and <t>HRP-conjugated</t> secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.
    Horseradish Peroxidase Hrp Conjugated Mouse Anti Goat Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated mouse anti goat antibodies/product/Agilent technologies
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated mouse anti goat antibodies - by Bioz Stars, 2020-09
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    92
    Agilent technologies goat anti mouse igg hrp
    Optimisation of culture conditions and r CLU expression in MEXi 293E cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse <t>IgG-CF488.</t> r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with <t>streptactin-HRP</t> conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p
    Goat Anti Mouse Igg Hrp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg hrp/product/Agilent technologies
    Average 92 stars, based on 2 article reviews
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    goat anti mouse igg hrp - by Bioz Stars, 2020-09
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    89
    Agilent technologies swine anti rabbit hrp
    <t>L2</t> content of PsV preparations. Denatured PsVs were serially diluted and added to the nitrocellulose membrane of the Quantitative Dot Blot (QDB) plate in quadruplicate. L1 and L2 were detected with ( A ) 16.D9 and ( B ) 16.L2.7I mAbs respectively and a secondary horseradish peroxidase <t>HRP</t> conjugated mAb. Plates were developed with enhanced chemiluminescence (ECL) peroxidase substrate and the chemiluminescent signal was read with a plate reader. Three separate assays were performed and representative linear regressions are shown. The ratios at the top three PsV concentrations were averaged to obtain a final ratio of 7:1 or approximately 50 L2 monomers.
    Swine Anti Rabbit Hrp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swine anti rabbit hrp/product/Agilent technologies
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    91
    Agilent technologies envision plus polymer anti mouse conjugated to hrp
    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse <t>HRP</t> polymer from <t>Agilent;</t> PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.
    Envision Plus Polymer Anti Mouse Conjugated To Hrp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/envision plus polymer anti mouse conjugated to hrp/product/Agilent technologies
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    Image Search Results


    ELISA competitive experiments showing that CIP inhibits the formation of the C3d–CR2/CD21 complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by anti-C3 pAb and HRP-conjugated secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: ELISA competitive experiments showing that CIP inhibits the formation of the C3d–CR2/CD21 complex. Microtiter wells were coated with 100 ng of CR2/CD21. The wells were probed with C3d preincubated with or without increasing concentrations of CIP or the highest concentration of Fib3 protein, followed by anti-C3 pAb and HRP-conjugated secondary antibody to detect the binding. Data are expressed as the percentage of the absorbance values detected in the absence of a competitor.

    Article Snippet: The membrane was probed with goat anti-C3 serum (Complement Technology, Tyler, TX, USA) and horseradish peroxidase (HRP)-conjugated mouse anti-goat antibodies (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Binding Assay

    Dose-dependent binding of CIP to surface-coated C3, C3b, and C3d. Microtiter wells were coated with 100 ng of complement factors and incubated with increasing concentrations of CIP; bound protein was detected by anti-CIP polyclonal serum, followed by an HRP-conjugated secondary antibody.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: Dose-dependent binding of CIP to surface-coated C3, C3b, and C3d. Microtiter wells were coated with 100 ng of complement factors and incubated with increasing concentrations of CIP; bound protein was detected by anti-CIP polyclonal serum, followed by an HRP-conjugated secondary antibody.

    Article Snippet: The membrane was probed with goat anti-C3 serum (Complement Technology, Tyler, TX, USA) and horseradish peroxidase (HRP)-conjugated mouse anti-goat antibodies (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Binding Assay, Incubation

    Far-Western blot analysis revealing the interaction between CIP and C3, C3b, C3d. A ) SDS-PAGE analysis of affinity-purified, His-tagged CIP used in this study. B ) CIP was subjected to SDS-PAGE, electroblotted onto a nitrocellulose membrane, and incubated either with normal (lane 1) or C3-depleted (lane 2) human serum; the membrane was probed with anti-C3 serum and HRP-conjugated secondary antibody. C ) C3, C3b, and C3d (lanes 1–3, respectively) were loaded onto SDS-PAGE, and the electroblotted membrane was overlaid with CIP, followed by a primary anti-CIP serum and then an HRP-conjugated secondary antibody to reveal the CIP binding. The expected MW of C3α, C3bα′, and C3β are indicated on the right.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: Far-Western blot analysis revealing the interaction between CIP and C3, C3b, C3d. A ) SDS-PAGE analysis of affinity-purified, His-tagged CIP used in this study. B ) CIP was subjected to SDS-PAGE, electroblotted onto a nitrocellulose membrane, and incubated either with normal (lane 1) or C3-depleted (lane 2) human serum; the membrane was probed with anti-C3 serum and HRP-conjugated secondary antibody. C ) C3, C3b, and C3d (lanes 1–3, respectively) were loaded onto SDS-PAGE, and the electroblotted membrane was overlaid with CIP, followed by a primary anti-CIP serum and then an HRP-conjugated secondary antibody to reveal the CIP binding. The expected MW of C3α, C3bα′, and C3β are indicated on the right.

    Article Snippet: The membrane was probed with goat anti-C3 serum (Complement Technology, Tyler, TX, USA) and horseradish peroxidase (HRP)-conjugated mouse anti-goat antibodies (Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Far Western Blot, SDS Page, Affinity Purification, Incubation, Binding Assay

    Optimisation of culture conditions and r CLU expression in MEXi 293E cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse IgG-CF488. r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with streptactin-HRP conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p

    Journal: Scientific Reports

    Article Title: Rapid high-yield expression and purification of fully post-translationally modified recombinant clusterin and mutants

    doi: 10.1038/s41598-020-70990-3

    Figure Lengend Snippet: Optimisation of culture conditions and r CLU expression in MEXi 293E cells. ( A ) and ( B ) Data shown for MEXi293E cells transfected with pEGFP-N1; means + /− SEM (n = 3) are plotted. In some cases the error bars are too small to be visible. ( A ) Left Panel: Viable cell density over 5 days post transfection (DPT). Right Panel: Percent cell viability over 5 DPT. ( B ) Percent of cells transiently expressing GFP measured for 5 DPT. ( C ) Transfection efficiency of cells overexpressing r CLU-αC-ST and r CLU-CT were measured by immunostaining of fixed, permeabilised cells on day 5 post-transfection. Untransfected (UN) cells were used as control. To detect r CLU-αC-ST, cells were stained with anti-strep tag antibody (Anti-ST Ab) or DNP9 (an isotype-matched antibody of irrelevant specificity) followed by goat anti-mouse IgG-CF488. r CLU-CT was detected using biotinylated anti-C tag conjugate ( b -CT-Ab) followed by streptavidin-CF488 (SA-488); the negative control in this case was cells incubated with SA-488 alone. ( D ) Densitometric analysis of Western blot with culture supernatant from cells overexpressing r CLU-αC-ST harvested on day 5 DPT and probed with streptactin-HRP conjugate. Cells were either supplemented with tryptone N1 (TN1) or sodium butyrate (NaB) or untreated (UN). Means + /− SEM (n = 3) are plotted; statistically significant differences are indicated by * (Oneway ANOVA, p

    Article Snippet: Bound primary antibodies were detected using goat anti-mouse IgG-HRP (Dako Agilent) diluted 1:5,000 in SM/PBS.

    Techniques: Expressing, Transfection, Immunostaining, Staining, Strep-tag, Negative Control, Incubation, Western Blot

    L2 content of PsV preparations. Denatured PsVs were serially diluted and added to the nitrocellulose membrane of the Quantitative Dot Blot (QDB) plate in quadruplicate. L1 and L2 were detected with ( A ) 16.D9 and ( B ) 16.L2.7I mAbs respectively and a secondary horseradish peroxidase HRP conjugated mAb. Plates were developed with enhanced chemiluminescence (ECL) peroxidase substrate and the chemiluminescent signal was read with a plate reader. Three separate assays were performed and representative linear regressions are shown. The ratios at the top three PsV concentrations were averaged to obtain a final ratio of 7:1 or approximately 50 L2 monomers.

    Journal: Viruses

    Article Title: Antibody Competition Reveals Surface Location of HPV L2 Minor Capsid Protein Residues 17–36

    doi: 10.3390/v9110336

    Figure Lengend Snippet: L2 content of PsV preparations. Denatured PsVs were serially diluted and added to the nitrocellulose membrane of the Quantitative Dot Blot (QDB) plate in quadruplicate. L1 and L2 were detected with ( A ) 16.D9 and ( B ) 16.L2.7I mAbs respectively and a secondary horseradish peroxidase HRP conjugated mAb. Plates were developed with enhanced chemiluminescence (ECL) peroxidase substrate and the chemiluminescent signal was read with a plate reader. Three separate assays were performed and representative linear regressions are shown. The ratios at the top three PsV concentrations were averaged to obtain a final ratio of 7:1 or approximately 50 L2 monomers.

    Article Snippet: The L2 capsid protein was detected using anti-L2 rabbit polyclonal serum (1:50) [ , ] and swine anti-rabbit HRP (1:15,000) (Dako-Agilent P0399, Santa Clara, CA, USA).

    Techniques: Quantitative Dot Blot

    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse HRP polymer from Agilent; PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.

    Journal: Journal of innate immunity

    Article Title: A role for neuronal alpha synuclein in gastrointestinal immunity

    doi: 10.1159/000477990

    Figure Lengend Snippet: Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse HRP polymer from Agilent; PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.

    Article Snippet: The secondary antibody was Envision Plus Polymer anti-mouse conjugated to HRP (Agilent K4001).

    Techniques: Staining, Immunofluorescence, Expressing, Plasmid Preparation, Amplification