avidin biotin blocking kit  (Vector Laboratories)


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    Name:
    Avidin Biotin Blocking Kit
    Description:
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    Catalog Number:
    sp-2001
    Price:
    None
    Category:
    Histology reagents or solutions or stains
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories avidin biotin blocking kit
    Avidin Biotin Blocking Kit
    Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
    https://www.bioz.com/result/avidin biotin blocking kit/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avidin biotin blocking kit - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Ultrastructural Visualization of 3D Chromatin Folding Using Serial Block-Face Scanning Electron Microscopy and In Situ Hybridization (3D-EMISH)
    Article Snippet: .. After blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). .. To augment permeabilization, the cells were immersed in a cryoprotectant solution (20% glycerol in PBS, RT, 2h) followed by their repeated freezing-thawing above the surface of the liquid nitrogen (4 × 30 sec).

    Article Title: Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization
    Article Snippet: .. In details, after blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min). ..

    Article Title: Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer
    Article Snippet: The secondary antibody (polyclonal rabbit anti-goat [RAGPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes, after which the tertiary antibody (polyclonal goat anti-rabbit [GARPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes. .. For VEGF-A, avidin/biotin was blocked using a Blocking Kit (Vector Laboratories SP-2001). .. Primary antibody was diluted in PBS containing 1% BSA and incubated for 1 hour at room temperature.

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Immunohistochemistry:

    Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling
    Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an avidin-biotin complex (ABC) kit purchased from Vector Laboratories [ , ]. ..

    Immunofluorescence:

    Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling
    Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an avidin-biotin complex (ABC) kit purchased from Vector Laboratories [ , ]. ..

    Avidin-Biotin Assay:

    Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling
    Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an avidin-biotin complex (ABC) kit purchased from Vector Laboratories [ , ]. ..

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: The sections were subsequently incubated with the biotinylated rabbit anti-sheep IgG (1:600; Vector Laboratories) for 1 h at room temperature. .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Article Title: Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer
    Article Snippet: The secondary antibody (polyclonal rabbit anti-goat [RAGPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes, after which the tertiary antibody (polyclonal goat anti-rabbit [GARPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes. .. For VEGF-A, avidin/biotin was blocked using a Blocking Kit (Vector Laboratories SP-2001). .. Primary antibody was diluted in PBS containing 1% BSA and incubated for 1 hour at room temperature.

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Article Title: Optimizing Glioblastoma Temozolomide Chemotherapy Employing Lentiviral-based Anti-MGMT shRNA Technology
    Article Snippet: Then, sections were incubated overnight at 4 °C with monoclonal mouse anti-MGMT antibody (dilution 1:25; MT3.1, ab39253; Abcam). .. Labeling of the primary antibody was performed using a commercial avidin-biotin complex detection kit based on a biotinylated anti-mouse antibody (PK-6102; Vector Laboratories, Loerrach, Germany) according to the manufacturers manual, followed by treatment with 3,3′-diaminobenzidine (DAB, D-5637; Sigma-Aldrich) for 5 minutes. .. Sections were counterstained with hematoxylin, dehydrated, and mounted using Entellan (Merck, Darmstadt, Germany).

    Article Title: Gene Transfer and Expression of Platelet-derived Growth Factors Modulate Periodontal Cellular Activity
    Article Snippet: After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL (Sigma Chemical Co., St. Louis, MO, USA), and blocked with 2% normal goat serum. .. Following treatment with primary antibody (1:50 dilution) specific for PDGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the cells were processed for peroxidase staining by means of a commercial avidin-biotin complex method kit (Vector Laboratories, Burlingame, CA, USA) and True Blue (KPL Laboratories, Gaithersburg, MD, USA) substrate. .. Mitogenic activity due to rAd delivery was evaluated by means of a standard BALB/c 3T3 bioassay as previously described for PDGF biological activity ( ).

    Amplification:

    Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD)
    Article Snippet: The sections were subsequently incubated with the biotinylated rabbit anti-sheep IgG (1:600; Vector Laboratories) for 1 h at room temperature. .. Signal amplification was performed using an avidin-biotin kit (1:100; Vector Laboratories) for 1 h at room temperature. ..

    Activity Assay:

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Binding Assay:

    Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment
    Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with avidin–biotin blocking kit according to manufacturer's instructions (Vector laboratories, SP‐2001). .. Primary antibody dilutions are as follows: pMLC‐2 (Cell Signaling, 3671, 1/200), α‐SMA (Abcam, ab5694, 1/300), CD68 (AbD Serotec, MCA1957GA, 1/500), YAP (Santa Cruz sc‐101199, 1/100).

    Labeling:

    Article Title: Optimizing Glioblastoma Temozolomide Chemotherapy Employing Lentiviral-based Anti-MGMT shRNA Technology
    Article Snippet: Then, sections were incubated overnight at 4 °C with monoclonal mouse anti-MGMT antibody (dilution 1:25; MT3.1, ab39253; Abcam). .. Labeling of the primary antibody was performed using a commercial avidin-biotin complex detection kit based on a biotinylated anti-mouse antibody (PK-6102; Vector Laboratories, Loerrach, Germany) according to the manufacturers manual, followed by treatment with 3,3′-diaminobenzidine (DAB, D-5637; Sigma-Aldrich) for 5 minutes. .. Sections were counterstained with hematoxylin, dehydrated, and mounted using Entellan (Merck, Darmstadt, Germany).

    Staining:

    Article Title: Gene Transfer and Expression of Platelet-derived Growth Factors Modulate Periodontal Cellular Activity
    Article Snippet: After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL (Sigma Chemical Co., St. Louis, MO, USA), and blocked with 2% normal goat serum. .. Following treatment with primary antibody (1:50 dilution) specific for PDGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the cells were processed for peroxidase staining by means of a commercial avidin-biotin complex method kit (Vector Laboratories, Burlingame, CA, USA) and True Blue (KPL Laboratories, Gaithersburg, MD, USA) substrate. .. Mitogenic activity due to rAd delivery was evaluated by means of a standard BALB/c 3T3 bioassay as previously described for PDGF biological activity ( ).

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  • 99
    Vector Laboratories vegf a
    HE, EGFR, GPNMB, and <t>VEGF</t> staining of the 3 types of lymph nodes. EGFR, epidermal growth factor receptor; GPNMB, glycoprotein nonmetastatic melanoma protein B; HE, hematoxylin and eosin; VEGF, vascular endothelial growth factor.
    Vegf A, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf a/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf a - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    HE, EGFR, GPNMB, and VEGF staining of the 3 types of lymph nodes. EGFR, epidermal growth factor receptor; GPNMB, glycoprotein nonmetastatic melanoma protein B; HE, hematoxylin and eosin; VEGF, vascular endothelial growth factor.

    Journal: Otolaryngology--Head and Neck Surgery

    Article Title: Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer

    doi: 10.1177/0194599820932869

    Figure Lengend Snippet: HE, EGFR, GPNMB, and VEGF staining of the 3 types of lymph nodes. EGFR, epidermal growth factor receptor; GPNMB, glycoprotein nonmetastatic melanoma protein B; HE, hematoxylin and eosin; VEGF, vascular endothelial growth factor.

    Article Snippet: For VEGF-A, avidin/biotin was blocked using a Blocking Kit (Vector Laboratories SP-2001).

    Techniques: Staining

    3D-EMISH method to visualize ultra-resolution 3D chromatin folding. a 3D-EMISH schematic. Cells are grown in suspension. After fixation with 4% PFA, thrombin–fibrinogen clot is formed. The clot is postfixed, soaked with cryoprotectant, frozen and cut in 40-µm sections. Free-floating sections are subjected to in situ hybridization with biotinylated DNA probe and processed to SBF-SEM. Then, multiple rounds of ultrathin slicing and imaging are performed. Each cubical sample volume contains dozens of cells. Cell nucleus is segmented, containing two separated target chromatins, as an example in 3D-EMISH. b Image processing for 3D-EMISH. First, we searched for the connected components through z -stack images per each identified nucleus (blue dotted circle). Second, the chromatin target structures were identified by removing nonspecific background EM signals. EM signals, connected in multiple consecutive layers were considered as actual target chromatin bound signals, otherwise regarded as chromatin unbound signals, or nonspecific signals. Two scale bars are 1 µm. c 3D-EMISH image example of one slice and all z -stack projected image after filtering out background EM signals. Two scale bars are 200 nm. d 3D reconstructed chromatin-folding structure examples. We assigned unique structure index number (sID) for each structure; scale bar, 500 nm.

    Journal: Nature Communications

    Article Title: Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization

    doi: 10.1038/s41467-020-15987-2

    Figure Lengend Snippet: 3D-EMISH method to visualize ultra-resolution 3D chromatin folding. a 3D-EMISH schematic. Cells are grown in suspension. After fixation with 4% PFA, thrombin–fibrinogen clot is formed. The clot is postfixed, soaked with cryoprotectant, frozen and cut in 40-µm sections. Free-floating sections are subjected to in situ hybridization with biotinylated DNA probe and processed to SBF-SEM. Then, multiple rounds of ultrathin slicing and imaging are performed. Each cubical sample volume contains dozens of cells. Cell nucleus is segmented, containing two separated target chromatins, as an example in 3D-EMISH. b Image processing for 3D-EMISH. First, we searched for the connected components through z -stack images per each identified nucleus (blue dotted circle). Second, the chromatin target structures were identified by removing nonspecific background EM signals. EM signals, connected in multiple consecutive layers were considered as actual target chromatin bound signals, otherwise regarded as chromatin unbound signals, or nonspecific signals. Two scale bars are 1 µm. c 3D-EMISH image example of one slice and all z -stack projected image after filtering out background EM signals. Two scale bars are 200 nm. d 3D reconstructed chromatin-folding structure examples. We assigned unique structure index number (sID) for each structure; scale bar, 500 nm.

    Article Snippet: In details, after blocking of endogenous biotin (Vector Laboratories; SP-2001), the cells were permeabilized with 0.5% Triton X-100 (Sigma) in PBS (RT, 20 min).

    Techniques: In Situ Hybridization, Imaging

    Development of sex cord-stromal tumors in TGFBR1-CA G9Cre mice. a-f Histological analysis of 8-week-old control and TGFBR1-CA G9Cre mice. Note the presence of hemorrhagic cysts ( c ; blue arrows) and hemorrhage ( d ; blue arrows) and neoplastic regions containing mitotic figures ( e and f ; red arrows) in TGFBR1-CA G9Cre ovaries compared with control ovaries ( a and b ). Panel f is a higher magnification image for the boxed region in panel ( e ). g and h Immunohistochemical analysis of Ki67 using 8-week-old TGFBR1-CA G9Cre ( g ) and control ( h ) ovaries. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Scale bar is representatively depicted in ( a ) and equals 12.5 μm ( f ), 25 μm ( e , g , and h ), 50 μm ( b ), and 250 μm ( a , c , and d ). H E staining and immunohistochemistry were conducted using 3-5 independent samples per group. i Gross ovarian tumor morphology of a 7-month-old mouse. Yellow arrows denote the ovary and ovarian tumors in the control and TGFBR1-CA G9Cre mice, respectively

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling

    doi: 10.1186/s12958-017-0312-z

    Figure Lengend Snippet: Development of sex cord-stromal tumors in TGFBR1-CA G9Cre mice. a-f Histological analysis of 8-week-old control and TGFBR1-CA G9Cre mice. Note the presence of hemorrhagic cysts ( c ; blue arrows) and hemorrhage ( d ; blue arrows) and neoplastic regions containing mitotic figures ( e and f ; red arrows) in TGFBR1-CA G9Cre ovaries compared with control ovaries ( a and b ). Panel f is a higher magnification image for the boxed region in panel ( e ). g and h Immunohistochemical analysis of Ki67 using 8-week-old TGFBR1-CA G9Cre ( g ) and control ( h ) ovaries. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Scale bar is representatively depicted in ( a ) and equals 12.5 μm ( f ), 25 μm ( e , g , and h ), 50 μm ( b ), and 250 μm ( a , c , and d ). H E staining and immunohistochemistry were conducted using 3-5 independent samples per group. i Gross ovarian tumor morphology of a 7-month-old mouse. Yellow arrows denote the ovary and ovarian tumors in the control and TGFBR1-CA G9Cre mice, respectively

    Article Snippet: Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an avidin-biotin complex (ABC) kit purchased from Vector Laboratories [ , ].

    Techniques: Mouse Assay, Immunohistochemistry, Staining

    Immunohistochemical detection of PDGF-AA protein by MG-63 osteogenic cells transduced with Ad2/PDGF-A. MG-63 cells were plated at subconfluence for 24 hrs in DMEM supplemented with 10% BCS. The medium was changed to DMEM supplemented with 5% platelet-poor plasma and cells transduced with either Ad2/GFP or Ad2/PDGF-A at MOI = 1 00. After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL, and blocked with 2% normal goat serum. Following treatment with primary antibody specific for PDGF-A, the cells were processed for peroxidase staining by means of a commercial avidin-biotin complex method kit and True Blue (KPL Laboratories, Gaithersburg, MD, USA) substrate. MG-63 cells transduced with Ad2/PDGF-A display positive staining consistent with PDGF-AA protein expression, while cells exposed to Ad2/GFP show a paucity of staining. Magnification, x20, phase contrast.

    Journal: Journal of dental research

    Article Title: Gene Transfer and Expression of Platelet-derived Growth Factors Modulate Periodontal Cellular Activity

    doi: 10.1177/00220345010800030901

    Figure Lengend Snippet: Immunohistochemical detection of PDGF-AA protein by MG-63 osteogenic cells transduced with Ad2/PDGF-A. MG-63 cells were plated at subconfluence for 24 hrs in DMEM supplemented with 10% BCS. The medium was changed to DMEM supplemented with 5% platelet-poor plasma and cells transduced with either Ad2/GFP or Ad2/PDGF-A at MOI = 1 00. After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL, and blocked with 2% normal goat serum. Following treatment with primary antibody specific for PDGF-A, the cells were processed for peroxidase staining by means of a commercial avidin-biotin complex method kit and True Blue (KPL Laboratories, Gaithersburg, MD, USA) substrate. MG-63 cells transduced with Ad2/PDGF-A display positive staining consistent with PDGF-AA protein expression, while cells exposed to Ad2/GFP show a paucity of staining. Magnification, x20, phase contrast.

    Article Snippet: Following treatment with primary antibody (1:50 dilution) specific for PDGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the cells were processed for peroxidase staining by means of a commercial avidin-biotin complex method kit (Vector Laboratories, Burlingame, CA, USA) and True Blue (KPL Laboratories, Gaithersburg, MD, USA) substrate.

    Techniques: Immunohistochemistry, Transduction, Staining, Avidin-Biotin Assay, Expressing