avidin biotin blocking kit (Vector Laboratories)


Name:
Avidin Biotin Blocking Kit
Description:
Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
Catalog Number:
sp-2001
Price:
None
Category:
Histology reagents or solutions or stains
Size:
1 Kit
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Structured Review

Avidin Biotin Blocking Kit blocks all endogenous biotin biotin receptors and avidin binding sites present in tissues This kit is designed for use with avidin detection systems such as the VECTASTAIN ABC Kits if avidin or biotinylated reagents bind non specifically to tissues or proteins This blocking kit consists of 18 ml of Avidin D and 18 ml of biotin in convenient dropper bottles
https://www.bioz.com/result/avidin biotin blocking kit/product/Vector Laboratories
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Blocking Assay:Article Title: Ultrastructural Visualization of 3D Chromatin Folding Using Serial Block-Face Scanning Electron Microscopy and In Situ Hybridization (3D-EMISH) Article Snippet: .. After blocking of Article Title: Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization Article Snippet: .. In details, after blocking of Article Title: Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer Article Snippet: The secondary antibody (polyclonal rabbit anti-goat [RAGPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes, after which the tertiary antibody (polyclonal goat anti-rabbit [GARPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes. .. For Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with Immunohistochemistry:Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an Immunofluorescence:Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an Avidin-Biotin Assay:Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling Article Snippet: .. Immunohistochemistry and Immunofluorescence Immunohistochemistry was performed using an Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD) Article Snippet: The sections were subsequently incubated with the biotinylated rabbit anti-sheep IgG (1:600; Vector Laboratories) for 1 h at room temperature. .. Signal amplification was performed using an Article Title: Glycoprotein Nonmetastatic Melanoma Protein B as Potential Imaging Marker in Posttherapeutic Metastatic Head and Neck Cancer Article Snippet: The secondary antibody (polyclonal rabbit anti-goat [RAGPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes, after which the tertiary antibody (polyclonal goat anti-rabbit [GARPO ], 1:100 diluted in PBS containing 1% BSA and 1% AB-serum; DAKO) was incubated for 30 minutes. .. For Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with Article Title: Optimizing Glioblastoma Temozolomide Chemotherapy Employing Lentiviral-based Anti-MGMT shRNA Technology Article Snippet: Then, sections were incubated overnight at 4 °C with monoclonal mouse anti-MGMT antibody (dilution 1:25; MT3.1, ab39253; Abcam). .. Labeling of the primary antibody was performed using a commercial Article Title: Gene Transfer and Expression of Platelet-derived Growth Factors Modulate Periodontal Cellular Activity Article Snippet: After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL (Sigma Chemical Co., St. Louis, MO, USA), and blocked with 2% normal goat serum. .. Following treatment with primary antibody (1:50 dilution) specific for PDGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the cells were processed for peroxidase staining by means of a commercial Amplification:Article Title: Effects of Estrogen Therapy on the Serotonergic System in an Animal Model of Perimenopause Induced by 4-Vinylcyclohexen Diepoxide (VCD) Article Snippet: The sections were subsequently incubated with the biotinylated rabbit anti-sheep IgG (1:600; Vector Laboratories) for 1 h at room temperature. .. Signal amplification was performed using an Activity Assay:Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with Binding Assay:Article Title: GPER is a mechanoregulator of pancreatic stellate cells and the tumor microenvironment Article Snippet: Endogenous hydrogen peroxide activity was quenched with 0.3% H2 O2 in methanol for 30 min at room temperature. .. Sections were blocked with normal serum for 1 h at room temperature and endogenous biotin activity and avidin binding sites were blocked with Labeling:Article Title: Optimizing Glioblastoma Temozolomide Chemotherapy Employing Lentiviral-based Anti-MGMT shRNA Technology Article Snippet: Then, sections were incubated overnight at 4 °C with monoclonal mouse anti-MGMT antibody (dilution 1:25; MT3.1, ab39253; Abcam). .. Labeling of the primary antibody was performed using a commercial Staining:Article Title: Gene Transfer and Expression of Platelet-derived Growth Factors Modulate Periodontal Cellular Activity Article Snippet: After 24 hrs, the cells were fixed with 2% paraformaldehyde, permeabilized with 0.5% IGEPAL (Sigma Chemical Co., St. Louis, MO, USA), and blocked with 2% normal goat serum. .. Following treatment with primary antibody (1:50 dilution) specific for PDGF-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the cells were processed for peroxidase staining by means of a commercial |