avidin biotin blocking kit  (Abcam)

 
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    Name:
    Endogenous Avidin Biotin Blocking Kit
    Description:

    Catalog Number:
    ab64212
    Price:
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    Structured Review

    Abcam avidin biotin blocking kit

    https://www.bioz.com/result/avidin biotin blocking kit/product/Abcam
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    avidin biotin blocking kit - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses
    Article Snippet: .. Sections were then again washed in PBS and blocked with the avidin/biotin blocking kit and were then processed for mouse-anti-calbindin immunohistochemistry (abcam, 1:250) and stained with the Vector VIP substrate (SK-4600) giving a violet color. .. Cell viability was assessed by lactate dehydrogenase (LDH) cytotoxicity assay (Roche) as previously described ( ).

    Article Title: Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed, rehydrated and treated sequentially with Avidin/Biotin Blocking Kit (Abcam), 3% H2 O2 and normal swine serum (Reactolab, Servion, Switzerland) to reduce non-specific staining. .. Sections were then incubated for 1 h at 37°C with either polyclonal rabbit anti-HTRA1 (1:300), polyclonal rabbit anti-HTRA3 (1:400), or equivalent dilutions of normal serum.

    Article Title: A C/EBPα–Wnt connection in gut homeostasis and carcinogenesis
    Article Snippet: .. After washing (PBS-T, 2 × 5 min), the sections were incubated with 10% normal serum (from the animal used to generate the subsequently used secondary antibody/ies) in PBS-T. For C/EBPα brightfield IHC, endogenous avidin/biotin blocking was performed as described in the kit manufacturer's manual (Abcam). .. All antibodies (C/EBPα, D56F10, Cell Signaling Technology, 1:100; mucin 2 [MUC2], H-300, Santa Cruz, 1:100; β-catenin, Clone14, BD Transduction Laboratories, 1:200; Ki67 for mouse clone TEC-3, for human clone MIB1, both Dako; GFP, Abcam, 1:400) were incubated at 4°C overnight in SignalStain antibody diluent (Cell Signaling Technology).

    Amplification:

    Article Title: Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis
    Article Snippet: .. An endogenous avidin/biotin blocking kit (Abcam) was used if biotin‐streptavidin amplification was employed. .. The same primary antibodies were used for FFPE as for frozen sections, aside from the mouse anti‐perforin antibody (5B10, dilution, 1:30; Dianova, Hamburg, Germany) and the rabbit anti‐CD163 antibody (EPR19518, dilution 1:200; Abcam).

    Avidin-Biotin Assay:

    Article Title: Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses
    Article Snippet: .. Sections were then again washed in PBS and blocked with the avidin/biotin blocking kit and were then processed for mouse-anti-calbindin immunohistochemistry (abcam, 1:250) and stained with the Vector VIP substrate (SK-4600) giving a violet color. .. Cell viability was assessed by lactate dehydrogenase (LDH) cytotoxicity assay (Roche) as previously described ( ).

    Article Title: Early Reactive A1 Astrocytes Induction by the Neurotoxin 3-Nitropropionic Acid in Rat Brain
    Article Snippet: .. Tissue sections were blocked with 1% BSA in PBS for 30 min, followed by incubation with 5% normal goat serum in 1% BSA and 0.1% Triton X-100 for 2 h. Next, slides were blocked with endogenous avidin/biotin blocking kit (Abcam ab 64212) and incubated overnight at 4 °C in humidified box, with primary antibodies: dilution 1:2000 for rabbit anti-C3 (Abcam ab225539), dilution 1:100 for rabbit anti-TNFα (Abcam ab6671), and dilution 1:500 for rabbit anti-neurogranin (Chemicon AB5620). .. After extensive washing in PBS, endogenous peroxidase activity was quenched with 0.5% H2 O2, again blocked, and incubated with the secondary antibody, a biotinylated goat anti-rabbit immunoglobulin G supplied by Vectastain ABC Kit, Vector Laboratories (PK-6101), for 3 h at room temperature.

    Article Title: Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed, rehydrated and treated sequentially with Avidin/Biotin Blocking Kit (Abcam), 3% H2 O2 and normal swine serum (Reactolab, Servion, Switzerland) to reduce non-specific staining. .. Sections were then incubated for 1 h at 37°C with either polyclonal rabbit anti-HTRA1 (1:300), polyclonal rabbit anti-HTRA3 (1:400), or equivalent dilutions of normal serum.

    Article Title: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
    Article Snippet: .. Non-specific binding was blocked with Endogenous Avidin/Biotin blocking kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s protocol followed by incubation in 1% bovine serum albumin (Sigma-Aldrich) in PBST (PBS + 1% Tween 20) for 1 h at room temperature. .. Cells were subsequently incubated with 1:1000 diluted primary antibody for C. jejuni (Biotin-rabbit polyclonal, Abcam) in PBST supplemented with 1% BSA in a humidified chamber overnight at 4 ℃.

    Article Title: Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis
    Article Snippet: .. An endogenous avidin/biotin blocking kit (Abcam) was used if biotin‐streptavidin amplification was employed. .. The same primary antibodies were used for FFPE as for frozen sections, aside from the mouse anti‐perforin antibody (5B10, dilution, 1:30; Dianova, Hamburg, Germany) and the rabbit anti‐CD163 antibody (EPR19518, dilution 1:200; Abcam).

    Article Title: Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning
    Article Snippet: .. Then an avidin/biotin blocking step was conducted using an Abcam endogenous avidin/biotin blocking kit. .. All the reformatted antibodies, and a commercial mouse antihuman CD117 (Clone YB5.B8, BD Biosciences, Sydney, Australia) known to cross-react with canine CD117 [ ], were diluted to 30 µg/mL in TBS, pH 7.4 (Abcam), while for the negative control section, TBS buffer alone substituted the primary antibody.

    Article Title: A C/EBPα–Wnt connection in gut homeostasis and carcinogenesis
    Article Snippet: .. After washing (PBS-T, 2 × 5 min), the sections were incubated with 10% normal serum (from the animal used to generate the subsequently used secondary antibody/ies) in PBS-T. For C/EBPα brightfield IHC, endogenous avidin/biotin blocking was performed as described in the kit manufacturer's manual (Abcam). .. All antibodies (C/EBPα, D56F10, Cell Signaling Technology, 1:100; mucin 2 [MUC2], H-300, Santa Cruz, 1:100; β-catenin, Clone14, BD Transduction Laboratories, 1:200; Ki67 for mouse clone TEC-3, for human clone MIB1, both Dako; GFP, Abcam, 1:400) were incubated at 4°C overnight in SignalStain antibody diluent (Cell Signaling Technology).

    Article Title: Cosyntropin Attenuates Neuroinflammation in a Mouse Model of Traumatic Brain Injury
    Article Snippet: .. For microglia/macrophage (Iba1) and neutrophil (MPO) cell counts, staining consisted of blocking endogenous peroxide activity with quenching buffer (10% methanol, 1% hydrogen peroxide in PBS) followed by blocking with Avidin/Biotin blocking Kit (AbCam, USA) and normal serum (5% Donkey and 5% Goat serum) with 1% Triton-X in PBS. .. Sections were incubated overnight with a polyclonal rabbit anti-Iba1 primary antibody (1:750, Rabbit anti-Iba1, Catalog no. 019-19741, Wako, USA) or monoclonal primary anti-MPO (1:800, Rabbit anti-Iba1, Catalog no. ab208670, Abcam, USA) and followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Vector).

    Blocking Assay:

    Article Title: Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses
    Article Snippet: .. Sections were then again washed in PBS and blocked with the avidin/biotin blocking kit and were then processed for mouse-anti-calbindin immunohistochemistry (abcam, 1:250) and stained with the Vector VIP substrate (SK-4600) giving a violet color. .. Cell viability was assessed by lactate dehydrogenase (LDH) cytotoxicity assay (Roche) as previously described ( ).

    Article Title: Early Reactive A1 Astrocytes Induction by the Neurotoxin 3-Nitropropionic Acid in Rat Brain
    Article Snippet: .. Tissue sections were blocked with 1% BSA in PBS for 30 min, followed by incubation with 5% normal goat serum in 1% BSA and 0.1% Triton X-100 for 2 h. Next, slides were blocked with endogenous avidin/biotin blocking kit (Abcam ab 64212) and incubated overnight at 4 °C in humidified box, with primary antibodies: dilution 1:2000 for rabbit anti-C3 (Abcam ab225539), dilution 1:100 for rabbit anti-TNFα (Abcam ab6671), and dilution 1:500 for rabbit anti-neurogranin (Chemicon AB5620). .. After extensive washing in PBS, endogenous peroxidase activity was quenched with 0.5% H2 O2, again blocked, and incubated with the secondary antibody, a biotinylated goat anti-rabbit immunoglobulin G supplied by Vectastain ABC Kit, Vector Laboratories (PK-6101), for 3 h at room temperature.

    Article Title: Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed, rehydrated and treated sequentially with Avidin/Biotin Blocking Kit (Abcam), 3% H2 O2 and normal swine serum (Reactolab, Servion, Switzerland) to reduce non-specific staining. .. Sections were then incubated for 1 h at 37°C with either polyclonal rabbit anti-HTRA1 (1:300), polyclonal rabbit anti-HTRA3 (1:400), or equivalent dilutions of normal serum.

    Article Title: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
    Article Snippet: .. Non-specific binding was blocked with Endogenous Avidin/Biotin blocking kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s protocol followed by incubation in 1% bovine serum albumin (Sigma-Aldrich) in PBST (PBS + 1% Tween 20) for 1 h at room temperature. .. Cells were subsequently incubated with 1:1000 diluted primary antibody for C. jejuni (Biotin-rabbit polyclonal, Abcam) in PBST supplemented with 1% BSA in a humidified chamber overnight at 4 ℃.

    Article Title: Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis
    Article Snippet: .. An endogenous avidin/biotin blocking kit (Abcam) was used if biotin‐streptavidin amplification was employed. .. The same primary antibodies were used for FFPE as for frozen sections, aside from the mouse anti‐perforin antibody (5B10, dilution, 1:30; Dianova, Hamburg, Germany) and the rabbit anti‐CD163 antibody (EPR19518, dilution 1:200; Abcam).

    Article Title: Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning
    Article Snippet: .. Then an avidin/biotin blocking step was conducted using an Abcam endogenous avidin/biotin blocking kit. .. All the reformatted antibodies, and a commercial mouse antihuman CD117 (Clone YB5.B8, BD Biosciences, Sydney, Australia) known to cross-react with canine CD117 [ ], were diluted to 30 µg/mL in TBS, pH 7.4 (Abcam), while for the negative control section, TBS buffer alone substituted the primary antibody.

    Article Title: A C/EBPα–Wnt connection in gut homeostasis and carcinogenesis
    Article Snippet: .. After washing (PBS-T, 2 × 5 min), the sections were incubated with 10% normal serum (from the animal used to generate the subsequently used secondary antibody/ies) in PBS-T. For C/EBPα brightfield IHC, endogenous avidin/biotin blocking was performed as described in the kit manufacturer's manual (Abcam). .. All antibodies (C/EBPα, D56F10, Cell Signaling Technology, 1:100; mucin 2 [MUC2], H-300, Santa Cruz, 1:100; β-catenin, Clone14, BD Transduction Laboratories, 1:200; Ki67 for mouse clone TEC-3, for human clone MIB1, both Dako; GFP, Abcam, 1:400) were incubated at 4°C overnight in SignalStain antibody diluent (Cell Signaling Technology).

    Article Title: Cosyntropin Attenuates Neuroinflammation in a Mouse Model of Traumatic Brain Injury
    Article Snippet: .. For microglia/macrophage (Iba1) and neutrophil (MPO) cell counts, staining consisted of blocking endogenous peroxide activity with quenching buffer (10% methanol, 1% hydrogen peroxide in PBS) followed by blocking with Avidin/Biotin blocking Kit (AbCam, USA) and normal serum (5% Donkey and 5% Goat serum) with 1% Triton-X in PBS. .. Sections were incubated overnight with a polyclonal rabbit anti-Iba1 primary antibody (1:750, Rabbit anti-Iba1, Catalog no. 019-19741, Wako, USA) or monoclonal primary anti-MPO (1:800, Rabbit anti-Iba1, Catalog no. ab208670, Abcam, USA) and followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Vector).

    Incubation:

    Article Title: Early Reactive A1 Astrocytes Induction by the Neurotoxin 3-Nitropropionic Acid in Rat Brain
    Article Snippet: .. Tissue sections were blocked with 1% BSA in PBS for 30 min, followed by incubation with 5% normal goat serum in 1% BSA and 0.1% Triton X-100 for 2 h. Next, slides were blocked with endogenous avidin/biotin blocking kit (Abcam ab 64212) and incubated overnight at 4 °C in humidified box, with primary antibodies: dilution 1:2000 for rabbit anti-C3 (Abcam ab225539), dilution 1:100 for rabbit anti-TNFα (Abcam ab6671), and dilution 1:500 for rabbit anti-neurogranin (Chemicon AB5620). .. After extensive washing in PBS, endogenous peroxidase activity was quenched with 0.5% H2 O2, again blocked, and incubated with the secondary antibody, a biotinylated goat anti-rabbit immunoglobulin G supplied by Vectastain ABC Kit, Vector Laboratories (PK-6101), for 3 h at room temperature.

    Article Title: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
    Article Snippet: .. Non-specific binding was blocked with Endogenous Avidin/Biotin blocking kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s protocol followed by incubation in 1% bovine serum albumin (Sigma-Aldrich) in PBST (PBS + 1% Tween 20) for 1 h at room temperature. .. Cells were subsequently incubated with 1:1000 diluted primary antibody for C. jejuni (Biotin-rabbit polyclonal, Abcam) in PBST supplemented with 1% BSA in a humidified chamber overnight at 4 ℃.

    Article Title: A C/EBPα–Wnt connection in gut homeostasis and carcinogenesis
    Article Snippet: .. After washing (PBS-T, 2 × 5 min), the sections were incubated with 10% normal serum (from the animal used to generate the subsequently used secondary antibody/ies) in PBS-T. For C/EBPα brightfield IHC, endogenous avidin/biotin blocking was performed as described in the kit manufacturer's manual (Abcam). .. All antibodies (C/EBPα, D56F10, Cell Signaling Technology, 1:100; mucin 2 [MUC2], H-300, Santa Cruz, 1:100; β-catenin, Clone14, BD Transduction Laboratories, 1:200; Ki67 for mouse clone TEC-3, for human clone MIB1, both Dako; GFP, Abcam, 1:400) were incubated at 4°C overnight in SignalStain antibody diluent (Cell Signaling Technology).

    Activity Assay:

    Article Title: Cosyntropin Attenuates Neuroinflammation in a Mouse Model of Traumatic Brain Injury
    Article Snippet: .. For microglia/macrophage (Iba1) and neutrophil (MPO) cell counts, staining consisted of blocking endogenous peroxide activity with quenching buffer (10% methanol, 1% hydrogen peroxide in PBS) followed by blocking with Avidin/Biotin blocking Kit (AbCam, USA) and normal serum (5% Donkey and 5% Goat serum) with 1% Triton-X in PBS. .. Sections were incubated overnight with a polyclonal rabbit anti-Iba1 primary antibody (1:750, Rabbit anti-Iba1, Catalog no. 019-19741, Wako, USA) or monoclonal primary anti-MPO (1:800, Rabbit anti-Iba1, Catalog no. ab208670, Abcam, USA) and followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Vector).

    Staining:

    Article Title: Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses
    Article Snippet: .. Sections were then again washed in PBS and blocked with the avidin/biotin blocking kit and were then processed for mouse-anti-calbindin immunohistochemistry (abcam, 1:250) and stained with the Vector VIP substrate (SK-4600) giving a violet color. .. Cell viability was assessed by lactate dehydrogenase (LDH) cytotoxicity assay (Roche) as previously described ( ).

    Article Title: Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation
    Article Snippet: .. Immunohistochemistry Tissue sections were dewaxed, rehydrated and treated sequentially with Avidin/Biotin Blocking Kit (Abcam), 3% H2 O2 and normal swine serum (Reactolab, Servion, Switzerland) to reduce non-specific staining. .. Sections were then incubated for 1 h at 37°C with either polyclonal rabbit anti-HTRA1 (1:300), polyclonal rabbit anti-HTRA3 (1:400), or equivalent dilutions of normal serum.

    Article Title: Cosyntropin Attenuates Neuroinflammation in a Mouse Model of Traumatic Brain Injury
    Article Snippet: .. For microglia/macrophage (Iba1) and neutrophil (MPO) cell counts, staining consisted of blocking endogenous peroxide activity with quenching buffer (10% methanol, 1% hydrogen peroxide in PBS) followed by blocking with Avidin/Biotin blocking Kit (AbCam, USA) and normal serum (5% Donkey and 5% Goat serum) with 1% Triton-X in PBS. .. Sections were incubated overnight with a polyclonal rabbit anti-Iba1 primary antibody (1:750, Rabbit anti-Iba1, Catalog no. 019-19741, Wako, USA) or monoclonal primary anti-MPO (1:800, Rabbit anti-Iba1, Catalog no. ab208670, Abcam, USA) and followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Vector).

    Binding Assay:

    Article Title: Campylobacter jejuni genes Cj1492c and Cj1507c are involved in host cell adhesion and invasion
    Article Snippet: .. Non-specific binding was blocked with Endogenous Avidin/Biotin blocking kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s protocol followed by incubation in 1% bovine serum albumin (Sigma-Aldrich) in PBST (PBS + 1% Tween 20) for 1 h at room temperature. .. Cells were subsequently incubated with 1:1000 diluted primary antibody for C. jejuni (Biotin-rabbit polyclonal, Abcam) in PBST supplemented with 1% BSA in a humidified chamber overnight at 4 ℃.

    Plasmid Preparation:

    Article Title: Nifedipine and nimodipine protect dopaminergic substantia nigra neurons against axotomy-induced cell death in rat vibrosections via modulating inflammatory responses
    Article Snippet: .. Sections were then again washed in PBS and blocked with the avidin/biotin blocking kit and were then processed for mouse-anti-calbindin immunohistochemistry (abcam, 1:250) and stained with the Vector VIP substrate (SK-4600) giving a violet color. .. Cell viability was assessed by lactate dehydrogenase (LDH) cytotoxicity assay (Roche) as previously described ( ).

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  • 95
    Abcam avidin biotin blocking kit
    Avidin Biotin Blocking Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avidin biotin blocking kit/product/Abcam
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    avidin biotin blocking kit - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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