avian myeloblastosis virus  (Thermo Fisher)


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    Structured Review

    Thermo Fisher avian myeloblastosis virus
    Detection of PERV in Hyate:C. Six lots of Hyate:C, labeled A through F, were examined for PERV by RT-PCR for PERV RNA pol sequences (A) or for RT activity by TM-PERT (B). The values for RT activity were derived from a standard curve generated using dilutions of a quantitated stock of avian <t>myeloblastosis</t> virus RT measured in the same assay. The assay baseline was 10 3 pU/ml.
    Avian Myeloblastosis Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Detection and Characterization of Porcine Endogenous Retrovirus in Porcine Plasma and Porcine Factor VIII"

    Article Title: Detection and Characterization of Porcine Endogenous Retrovirus in Porcine Plasma and Porcine Factor VIII

    Journal: Journal of Virology

    doi: 10.1128/JVI.75.10.4551-4557.2001

    Detection of PERV in Hyate:C. Six lots of Hyate:C, labeled A through F, were examined for PERV by RT-PCR for PERV RNA pol sequences (A) or for RT activity by TM-PERT (B). The values for RT activity were derived from a standard curve generated using dilutions of a quantitated stock of avian myeloblastosis virus RT measured in the same assay. The assay baseline was 10 3 pU/ml.
    Figure Legend Snippet: Detection of PERV in Hyate:C. Six lots of Hyate:C, labeled A through F, were examined for PERV by RT-PCR for PERV RNA pol sequences (A) or for RT activity by TM-PERT (B). The values for RT activity were derived from a standard curve generated using dilutions of a quantitated stock of avian myeloblastosis virus RT measured in the same assay. The assay baseline was 10 3 pU/ml.

    Techniques Used: Labeling, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Derivative Assay, Generated

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Detection of Murine Leukemia Virus or Mouse DNA in Commercial RT-PCR Reagents and Human DNAs
    Article Snippet: .. Increasing amounts of AMV RT from the Finnzymes kit used in the nested pol PCR test also yielded increasing quantities of detectable contaminants (5/16, 31%). .. We also replaced the AMV RT from Finnzymes with an AMV RT from Promega and repeated the testing and found no positive reactions in the water only controls (0/16, data not shown), further supporting the contamination of the Finnzymes RT.

    Article Title: Detection of Murine Leukemia Virus or Mouse DNA in Commercial RT-PCR Reagents and Human DNAs
    Article Snippet: .. Thus, the nested pol PCR assay was used to test the RT in the Finnzymes kit. shows the detection of pol sequences in 2/16 (12.5%) replicates in the AMV RT from Finnzymes. .. Increasing amounts of AMV RT from the Finnzymes kit used in the nested pol PCR test also yielded increasing quantities of detectable contaminants (5/16, 31%).

    Clone Assay:

    Article Title: Rapamycin-induced modulation of HIV gene transcription attenuates progression of HIVAN
    Article Snippet: .. Subsequently, 8μl of the reverse-transcription (RT) reaction mixture containing cloned AMV RT, 0.5 mmol each of the mixed nucleotides, 0.01 mol dithiothreitol, and 1000 U/mL Rnasin (Invitrogen Corp) was incubated at 42°C for 50 min. For a negative control, a reaction mixture without RNA or reverse transcription (RT) was used. .. Quantitative PCR was carried out in an ABI Prism 7900HT sequence detection system using the primer sequences as shown below:SYBR green was used as the detector and ROX as a stablizing dye.

    Incubation:

    Article Title: Rapamycin-induced modulation of HIV gene transcription attenuates progression of HIVAN
    Article Snippet: .. Subsequently, 8μl of the reverse-transcription (RT) reaction mixture containing cloned AMV RT, 0.5 mmol each of the mixed nucleotides, 0.01 mol dithiothreitol, and 1000 U/mL Rnasin (Invitrogen Corp) was incubated at 42°C for 50 min. For a negative control, a reaction mixture without RNA or reverse transcription (RT) was used. .. Quantitative PCR was carried out in an ABI Prism 7900HT sequence detection system using the primer sequences as shown below:SYBR green was used as the detector and ROX as a stablizing dye.

    Negative Control:

    Article Title: Rapamycin-induced modulation of HIV gene transcription attenuates progression of HIVAN
    Article Snippet: .. Subsequently, 8μl of the reverse-transcription (RT) reaction mixture containing cloned AMV RT, 0.5 mmol each of the mixed nucleotides, 0.01 mol dithiothreitol, and 1000 U/mL Rnasin (Invitrogen Corp) was incubated at 42°C for 50 min. For a negative control, a reaction mixture without RNA or reverse transcription (RT) was used. .. Quantitative PCR was carried out in an ABI Prism 7900HT sequence detection system using the primer sequences as shown below:SYBR green was used as the detector and ROX as a stablizing dye.

    Isolation:

    Article Title: A large animal model of Spinal Muscular Atrophy and correction of phenotype
    Article Snippet: .. RNA was isolated using the RNaqueous Micro Kit (Ambion) according to the manufacturer’s instructions and cDNA was obtained using AMV-RT (Invitrogen) as described by manufacturer. ..

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    Thermo Fisher amv reverse transcriptase
    Amv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amv rnas
    Accumulation of the three genomic segments of <t>AMV</t> on transgenic N. benthamiana plants that express viral proteins P1, P2 or both. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( B ) Normalized frequency ternary plot showing the effect of transgenic expression of viral <t>RNAs</t> in the estimated SGF . ( C ) As in ( A ) but determined for encapsidated RNAs. ( D ) As in ( B ) but for encapsidated RNAs.
    Amv Rnas, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amv rt polymerase
    Accumulation of the three genomic segments of <t>AMV</t> on transgenic N. benthamiana plants that express viral proteins P1, P2 or both. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( B ) Normalized frequency ternary plot showing the effect of transgenic expression of viral <t>RNAs</t> in the estimated SGF . ( C ) As in ( A ) but determined for encapsidated RNAs. ( D ) As in ( B ) but for encapsidated RNAs.
    Amv Rt Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Accumulation of the three genomic segments of AMV on transgenic N. benthamiana plants that express viral proteins P1, P2 or both. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( B ) Normalized frequency ternary plot showing the effect of transgenic expression of viral RNAs in the estimated SGF . ( C ) As in ( A ) but determined for encapsidated RNAs. ( D ) As in ( B ) but for encapsidated RNAs.

    Journal: Scientific Reports

    Article Title: Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus

    doi: 10.1038/s41598-017-05335-8

    Figure Lengend Snippet: Accumulation of the three genomic segments of AMV on transgenic N. benthamiana plants that express viral proteins P1, P2 or both. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( B ) Normalized frequency ternary plot showing the effect of transgenic expression of viral RNAs in the estimated SGF . ( C ) As in ( A ) but determined for encapsidated RNAs. ( D ) As in ( B ) but for encapsidated RNAs.

    Article Snippet: The quantification of the AMV RNAs was performed with a ND-1000 spectrophotometer (Thermo Scientific, USA) and agarose gel eletrophoresis using an RNA ladder (RiboRuler High Range RNA Ladder 200 to 6000, Thermo Scientific) and several dilutions of the transcribed RNAs.

    Techniques: Transgenic Assay, Quantitative RT-PCR, Expressing

    Effect of host species in the accumulation of each genomic segment of AMV. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants per hsot species; error bars represent ±1 SEM. ( B ) As in ( A ) but determined for encapsidated RNAs. No data are available for C. annuum and M. sativa . ( C ) Normalized frequency ternary plot showing the effect of host species in the estimated SGF . Solid symbols represent the marginal mean frequencies estimated from total RNA samples. Open symbols represent the marginal mean frequencies estimated from virion RNA samples. Lines crossing symbols represent 95% CIs.

    Journal: Scientific Reports

    Article Title: Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus

    doi: 10.1038/s41598-017-05335-8

    Figure Lengend Snippet: Effect of host species in the accumulation of each genomic segment of AMV. ( A ) Experimental determinations (by RT-qPCR) of the frequency of each AMV genomic in total RNA extractions from five different host species. Bars represent the mean of n = 3 plants per hsot species; error bars represent ±1 SEM. ( B ) As in ( A ) but determined for encapsidated RNAs. No data are available for C. annuum and M. sativa . ( C ) Normalized frequency ternary plot showing the effect of host species in the estimated SGF . Solid symbols represent the marginal mean frequencies estimated from total RNA samples. Open symbols represent the marginal mean frequencies estimated from virion RNA samples. Lines crossing symbols represent 95% CIs.

    Article Snippet: The quantification of the AMV RNAs was performed with a ND-1000 spectrophotometer (Thermo Scientific, USA) and agarose gel eletrophoresis using an RNA ladder (RiboRuler High Range RNA Ladder 200 to 6000, Thermo Scientific) and several dilutions of the transcribed RNAs.

    Techniques: Quantitative RT-PCR

    Effect of differences in the input ratio of RNA segments on the outcome of AMV infection. ( A ) Scheme of the sampling process, with indication of the three leafs sampled. ( B ) Experimental determinations (by RT-qPCR) of the frequency of each one of the three RNA segments in samples of total RNA for different input ratios. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( C ) Normalized frequency ternary plot showing the estimated SGF . Solid circles represent the indicated inoculation rates. Open circles show the marginal mean estimates of relative ratios at the end of the experiment corresponding to each input ratio. Lines crossing the open circles represent the 95% Cis. Red arrows connect initial and final ratios. ( D ) As in ( B ) but determined for encapsidated RNAs. ( E ) As in ( C ) but for encapsidated RNAs.

    Journal: Scientific Reports

    Article Title: Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus

    doi: 10.1038/s41598-017-05335-8

    Figure Lengend Snippet: Effect of differences in the input ratio of RNA segments on the outcome of AMV infection. ( A ) Scheme of the sampling process, with indication of the three leafs sampled. ( B ) Experimental determinations (by RT-qPCR) of the frequency of each one of the three RNA segments in samples of total RNA for different input ratios. Bars represent the mean of n = 3 plants; error bars represent ±1 SEM. ( C ) Normalized frequency ternary plot showing the estimated SGF . Solid circles represent the indicated inoculation rates. Open circles show the marginal mean estimates of relative ratios at the end of the experiment corresponding to each input ratio. Lines crossing the open circles represent the 95% Cis. Red arrows connect initial and final ratios. ( D ) As in ( B ) but determined for encapsidated RNAs. ( E ) As in ( C ) but for encapsidated RNAs.

    Article Snippet: The quantification of the AMV RNAs was performed with a ND-1000 spectrophotometer (Thermo Scientific, USA) and agarose gel eletrophoresis using an RNA ladder (RiboRuler High Range RNA Ladder 200 to 6000, Thermo Scientific) and several dilutions of the transcribed RNAs.

    Techniques: Infection, Sampling, Quantitative RT-PCR