avian myeloblastosis virus  (TaKaRa)

 
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    TaKaRa avian myeloblastosis virus
    Avian Myeloblastosis Virus, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus/product/TaKaRa
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus - by Bioz Stars, 2020-08
    89/100 stars

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    Amplification:

    Article Title: Release of heat shock protein 70 and the effects of extracellular heat shock protein 70 on the production of IL-10 in fibroblast-like synoviocytes
    Article Snippet: .. Total RNA (1 μg) was then reverse transcribed with avian myeloblastosis virus and oligo (dT) primer (Takara, Dalian, China) and amplified in a final volume of 20 μl containing 1 μl RT mix template, 1 U Taq DNA polymerase (Takara), and 10 pmol of each primer (sense and antisense). .. For human TLR2 amplification, the primers 5′-GCCAAAGTCTTGATTGATTGG-3′ and 5′-TTGAAGTTCTCCAGCTCCTG-3′ were used.

    Concentration Assay:

    Article Title: Angiogenesis in rabbit hepatic tumor after transcatheter arterial embolization
    Article Snippet: .. The RNA concentration and quality were determined by UV spectrophotometer at absorbances of 260 and 280 nm. cDNA was synthesized from total RNA with Avian Myeloblastosis Virus and oligo dT-Adaptor Primer (TaKaRa RNA PCR Kit, TaKaRa Bioteth., Dalian, CHN) according to the manufacturer’s instructions. .. Amplification of cDNA was performed using published sense and antisense primers for rabbit β-actin and VEGF[ , , ].

    Synthesized:

    Article Title: Angiogenesis in rabbit hepatic tumor after transcatheter arterial embolization
    Article Snippet: .. The RNA concentration and quality were determined by UV spectrophotometer at absorbances of 260 and 280 nm. cDNA was synthesized from total RNA with Avian Myeloblastosis Virus and oligo dT-Adaptor Primer (TaKaRa RNA PCR Kit, TaKaRa Bioteth., Dalian, CHN) according to the manufacturer’s instructions. .. Amplification of cDNA was performed using published sense and antisense primers for rabbit β-actin and VEGF[ , , ].

    Spectrophotometry:

    Article Title: Angiogenesis in rabbit hepatic tumor after transcatheter arterial embolization
    Article Snippet: .. The RNA concentration and quality were determined by UV spectrophotometer at absorbances of 260 and 280 nm. cDNA was synthesized from total RNA with Avian Myeloblastosis Virus and oligo dT-Adaptor Primer (TaKaRa RNA PCR Kit, TaKaRa Bioteth., Dalian, CHN) according to the manufacturer’s instructions. .. Amplification of cDNA was performed using published sense and antisense primers for rabbit β-actin and VEGF[ , , ].

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phylogenetic Analysis of the Entire Genome of Influenza A (H3N2) Viruses from Japan: Evidence for Genetic Reassortment of the Six Internal Genes
    Article Snippet: .. Reverse transcription (RT)-PCR was performed by using a slightly modified protocol of a commercial kit (RT-PCR kit with avian myeloblastosis virus [AMV]; version 2.1; Takara). .. Briefly, first-strand cDNA synthesis was done by mixing 9.5 μl of RNA with 1 μl of “influenza A universal RT” primer (3′-AGCAAAAGCAGG-5′) (20 μM) and 9.5 μl of RT reaction mixture (4 μl of 25 mM MgCl2 , 2 μl of 10× RT-PCR buffer, 2 μl of 10 mM dNTP, 1 μl of AMV reverse transcriptase, 0.5 μl of RNase inhibitor).

    Polymerase Chain Reaction:

    Article Title: Angiogenesis in rabbit hepatic tumor after transcatheter arterial embolization
    Article Snippet: .. The RNA concentration and quality were determined by UV spectrophotometer at absorbances of 260 and 280 nm. cDNA was synthesized from total RNA with Avian Myeloblastosis Virus and oligo dT-Adaptor Primer (TaKaRa RNA PCR Kit, TaKaRa Bioteth., Dalian, CHN) according to the manufacturer’s instructions. .. Amplification of cDNA was performed using published sense and antisense primers for rabbit β-actin and VEGF[ , , ].

    Modification:

    Article Title: Phylogenetic Analysis of the Entire Genome of Influenza A (H3N2) Viruses from Japan: Evidence for Genetic Reassortment of the Six Internal Genes
    Article Snippet: .. Reverse transcription (RT)-PCR was performed by using a slightly modified protocol of a commercial kit (RT-PCR kit with avian myeloblastosis virus [AMV]; version 2.1; Takara). .. Briefly, first-strand cDNA synthesis was done by mixing 9.5 μl of RNA with 1 μl of “influenza A universal RT” primer (3′-AGCAAAAGCAGG-5′) (20 μM) and 9.5 μl of RT reaction mixture (4 μl of 25 mM MgCl2 , 2 μl of 10× RT-PCR buffer, 2 μl of 10 mM dNTP, 1 μl of AMV reverse transcriptase, 0.5 μl of RNase inhibitor).

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    • rna pcr kit amv  (TaKaRa)
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    TaKaRa rna pcr kit amv
    pTT5-Act1 expression vector construction. (A) Total <t>RNA</t> extraction from Raji cells. (B) A gel extraction kit was used to purify cDNA fragments of Act1 and pTT5 following dual restriction enzyme digestion (M, Marker; 1, 2 and 3, polymerase chain reaction products of Act1). (C) Construction of the pTT5-Act1 expression vector (M, Marker; 1, 2, 3 and 5, recombinant plasmid of pTT5-Act1). (D) A positive clone of pTT5-Act1 was verified using dual digestion (M, Marker; 1 and 2, sequences of pTT5 and Act1). Act1, nuclear factor-κB Activator 1.
    Rna Pcr Kit Amv, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna pcr kit amv/product/TaKaRa
    Average 96 stars, based on 884 article reviews
    Price from $9.99 to $1999.99
    rna pcr kit amv - by Bioz Stars, 2020-08
    96/100 stars
    		  Buy from Supplier

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    pTT5-Act1 expression vector construction. (A) Total RNA extraction from Raji cells. (B) A gel extraction kit was used to purify cDNA fragments of Act1 and pTT5 following dual restriction enzyme digestion (M, Marker; 1, 2 and 3, polymerase chain reaction products of Act1). (C) Construction of the pTT5-Act1 expression vector (M, Marker; 1, 2, 3 and 5, recombinant plasmid of pTT5-Act1). (D) A positive clone of pTT5-Act1 was verified using dual digestion (M, Marker; 1 and 2, sequences of pTT5 and Act1). Act1, nuclear factor-κB Activator 1.

    Journal: Oncology Letters

    Article Title: Regulatory effect of Act1 on the BAFF pathway in B-cell malignancy

    doi: 10.3892/ol.2019.10047

    Figure Lengend Snippet: pTT5-Act1 expression vector construction. (A) Total RNA extraction from Raji cells. (B) A gel extraction kit was used to purify cDNA fragments of Act1 and pTT5 following dual restriction enzyme digestion (M, Marker; 1, 2 and 3, polymerase chain reaction products of Act1). (C) Construction of the pTT5-Act1 expression vector (M, Marker; 1, 2, 3 and 5, recombinant plasmid of pTT5-Act1). (D) A positive clone of pTT5-Act1 was verified using dual digestion (M, Marker; 1 and 2, sequences of pTT5 and Act1). Act1, nuclear factor-κB Activator 1.

    Article Snippet: RNA isolation and reverse transcription-quantittive polymerase chain reaction (RT-qPCR) Total RNA was extracted from cells using the RNA PCR kit (AMV), version 3.0 (Takara Bio, Inc., Otsu, Japan) following the manufacturer's protocols.

    Techniques: Expressing, Plasmid Preparation, RNA Extraction, Gel Extraction, Marker, Polymerase Chain Reaction, Recombinant

    The expression patterns of CXCL12 and CXCR4 in breast cancer cell lines. Total RNA and proteins were extracted from wild-type MCF-7, wild-type MDA-MB-435s, wild-type MDA-MB-231, MDA-MB-231–CXCL12 and MDA-MB-231–ZsGreen1. Then they were subjected to RT-PCR and western blot analysis, respectively, for CXCL12 and CXCR4. Both mRNA ( a ) and protein ( b ) expression of CXCR4 and very weak expression of CXCL12 were observed consistently in MDA-MB-231. CXCR4 and CXCL12 mRNA ( a ) and protein ( b ) expression was found in MDA-MB-435s. MCF-7 has very weak CXCR4 and CXCL12 mRNA and protein expression ( a and b ). So we picked MDA-MB-231 to be transfected with CXCL12. CXCL12 mRNA ( a ) and protein ( b ) expression was observed in MDA-MB-231–CXCL12 cells, but very weak in MDA-MB-231–ZsGreen1 or wild-type MDA-MB-231 cells. It is indicated that the stable CXCL12 transfection in MDA-MB-231 was successfully constructed

    Journal: Tumour Biology

    Article Title: CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis

    doi: 10.1007/s13277-014-1816-1

    Figure Lengend Snippet: The expression patterns of CXCL12 and CXCR4 in breast cancer cell lines. Total RNA and proteins were extracted from wild-type MCF-7, wild-type MDA-MB-435s, wild-type MDA-MB-231, MDA-MB-231–CXCL12 and MDA-MB-231–ZsGreen1. Then they were subjected to RT-PCR and western blot analysis, respectively, for CXCL12 and CXCR4. Both mRNA ( a ) and protein ( b ) expression of CXCR4 and very weak expression of CXCL12 were observed consistently in MDA-MB-231. CXCR4 and CXCL12 mRNA ( a ) and protein ( b ) expression was found in MDA-MB-435s. MCF-7 has very weak CXCR4 and CXCL12 mRNA and protein expression ( a and b ). So we picked MDA-MB-231 to be transfected with CXCL12. CXCL12 mRNA ( a ) and protein ( b ) expression was observed in MDA-MB-231–CXCL12 cells, but very weak in MDA-MB-231–ZsGreen1 or wild-type MDA-MB-231 cells. It is indicated that the stable CXCL12 transfection in MDA-MB-231 was successfully constructed

    Article Snippet: The reverse transcription was performed with RNA PCR kit (AMV ver.3.0, Takara, Japan) according to the manufacturer's protocols.

    Techniques: Expressing, Multiple Displacement Amplification, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Construct

    Expression patterns of RAP80 in breast cancer cell lines. Notes: Total RNA and proteins were extracted from wild-type MCF-7, ZR-75, and MDA-MB-231, then subjected to qRT-PCR and Western blotting for RAP80. Both protein ( A – D ) and mRNA ( C , E ) expression of RAP80 were observed consistently in MCF-7, but ZR-75 and MDA-MB-231 had very weak RAP80 mRNA and protein expression. Therefore, we picked MCF-7 for transfection with RAP80 siRNA. Data are presented as mean ± SD of three independent experiments, * P

    Journal: OncoTargets and therapy

    Article Title: RAP80 expression in breast cancer and its relationship with apoptosis in breast cancer cells

    doi: 10.2147/OTT.S186981

    Figure Lengend Snippet: Expression patterns of RAP80 in breast cancer cell lines. Notes: Total RNA and proteins were extracted from wild-type MCF-7, ZR-75, and MDA-MB-231, then subjected to qRT-PCR and Western blotting for RAP80. Both protein ( A – D ) and mRNA ( C , E ) expression of RAP80 were observed consistently in MCF-7, but ZR-75 and MDA-MB-231 had very weak RAP80 mRNA and protein expression. Therefore, we picked MCF-7 for transfection with RAP80 siRNA. Data are presented as mean ± SD of three independent experiments, * P

    Article Snippet: The reverse transcription was performed with RNA PCR Kit (AMV Ver.3.0, Takara, Kyoto, Japan) according to the manufacturer’s protocols. qRT-PCR was performed by SYBR® Premix Ex TaqTM II Kit (Takara) using 7500 Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific).

    Techniques: Expressing, Multiple Displacement Amplification, Quantitative RT-PCR, Western Blot, Transfection

    RNA extraction and semi-quantitative RT-PCR

    Journal: Acta Pharmacologica Sinica

    Article Title: Exendin-4 ameliorates oxidized-LDL-induced inhibition of macrophage migration in vitro via the NF-κB pathway

    doi: 10.1038/aps.2013.128

    Figure Lengend Snippet: RNA extraction and semi-quantitative RT-PCR

    Article Snippet: TaKaRa RNA PCR Kit (AMV; version 3.0) was acquired from Takara Biotechnology Co, Ltd (Dalian, China).

    Techniques: RNA Extraction, Quantitative RT-PCR