Structured Review

Roche avian myeloblastosis virus
Avian Myeloblastosis Virus, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avian myeloblastosis virus/product/Roche
Average 89 stars, based on 12 article reviews
Price from $9.99 to $1999.99
avian myeloblastosis virus - by Bioz Stars, 2020-08
89/100 stars

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Amplification:

Article Title: Multifactorial Interplay Controls the Splicing Profile of Alu-Derived Exons
Article Snippet: .. Reverse transcription-PCR (RT-PCR) amplification was performed for 1 h at 42°C, using an oligo(dT) reverse primer and 2 U reverse transcriptase from avian myeloblastosis virus (Roche). .. The spliced cDNA products derived from the expressed minigenes were detected by PCR.

Polymerase Chain Reaction:

Article Title: Effects of dopamine on leptin release and leptin gene (OB) expression in adipocytes from obese and hypertensive patients
Article Snippet: .. Using avian myeloblastosis virus for first-strand synthesis and the Expand High-Fidelity enzyme blend (Roche), which consisted of Taq DNA polymerase and Pwo DNA polymerase for the PCR part, a one-step reaction system was performed. .. In addition, the system included a single optimized RT-PCR buffer, control RNA from a human cell line (K562), and control primers for human β-actin messenger RNA (mRNA).

Real-time Polymerase Chain Reaction:

Article Title: MBD3, a Component of the NuRD Complex, Facilitates Chromatin Alteration and Deposition of Epigenetic Marks ▿
Article Snippet: .. RNA from U937-PR9 MBD3 RNA interference cells (RNAi MBD3) or from U937-PR9 control cells (RNAi control) after no treatment, after RA treatment (1 nM, 36 h), and after RA treatment (1 nM, 12 h) with subsequent Zn induction (100 mM Zn, 24 h) was extracted by using a Qiagen RNeasy minikit, retrotranscripted (avian myeloblastosis virus; Roche), and assayed for the expression of RARβ2 by using quantitative real-time PCR (Roche LightCycler). .. The sequences of the PCR primers are available upon request.

Sequencing:

Article Title: Expression and disruption of the Arabidopsis TOR (target of rapamycin) gene
Article Snippet: .. The entire AtTOR coding sequence was reverse transcribed from 1 μg of total RNAs from wild-type roots (ecotype Columbia) with reverse transcriptase from avian myeloblastosis virus (Roche Molecular Biochemicals) and primer TOR-R1 (5′-GCGGCCGCAAATGCAAATTAGTTGA-3′). .. The RT product was amplified by PCR (9 min of elongation) with the Expand Long Template System (Roche Molecular Biochemicals) and primers TOR R1 and TOR 7 (5′-CCTGCATCCATGGCTTCCCCTTC-3′).

Expressing:

Article Title: MBD3, a Component of the NuRD Complex, Facilitates Chromatin Alteration and Deposition of Epigenetic Marks ▿
Article Snippet: .. RNA from U937-PR9 MBD3 RNA interference cells (RNAi MBD3) or from U937-PR9 control cells (RNAi control) after no treatment, after RA treatment (1 nM, 36 h), and after RA treatment (1 nM, 12 h) with subsequent Zn induction (100 mM Zn, 24 h) was extracted by using a Qiagen RNeasy minikit, retrotranscripted (avian myeloblastosis virus; Roche), and assayed for the expression of RARβ2 by using quantitative real-time PCR (Roche LightCycler). .. The sequences of the PCR primers are available upon request.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Multifactorial Interplay Controls the Splicing Profile of Alu-Derived Exons
Article Snippet: .. Reverse transcription-PCR (RT-PCR) amplification was performed for 1 h at 42°C, using an oligo(dT) reverse primer and 2 U reverse transcriptase from avian myeloblastosis virus (Roche). .. The spliced cDNA products derived from the expressed minigenes were detected by PCR.

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  • 92
    Roche amv reverse transcriptase
    Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with <t>[α-</t> 32 P]dATP in the presence of <t>AMV</t> reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.
    Amv Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase/product/Roche
    Average 92 stars, based on 140 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
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    amv  (Roche)
    91
    Roche amv
    Gel electrophoresis on <t>RT-PCR</t> amplification of a fragment from <t>AMV</t> genome using specific primer pair designed to amplify 700 bp fragment of CP gene. Lane M represents Hyper-Laddert II (Bioline), Lane 1–4 (Sample 1–4 from infected alfalfa plants collected from Sajer location), Lane 6–11 (Leaf sample from symptomatic alfalfa plants collected from Wadi aldawasser location), and Lane 5 (healthy alfalfa as a negative control.
    Amv, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv/product/Roche
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    amv - by Bioz Stars, 2020-08
    91/100 stars
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    93
    Roche first strand cdna synthesis kit
    RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. <t>cDNA</t> was generated from mRNA by using the oligonucleotide <t>SalRterm.</t> (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).
    First Strand Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna synthesis kit/product/Roche
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    first strand cdna synthesis kit - by Bioz Stars, 2020-08
    93/100 stars
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    92
    Roche avian myeloblastosis virus reverse transcriptase
    Extension of an std mRNA primer with avian <t>myeloblastosis</t> virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Roche
    Average 92 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with [α- 32 P]dATP in the presence of AMV reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.

    Journal: Nucleic Acids Research

    Article Title: DNA sequence recognition by the indolocarbazole antitumor antibiotic AT2433-B1 and its diastereoisomer

    doi:

    Figure Lengend Snippet: Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with [α- 32 P]dATP in the presence of AMV reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.

    Article Snippet: The 117 bp DNA fragment was prepared by 3′-32 P-end-labeling of an Eco RI + Pvu II double digest of plasmid pBS (Stratagene) using [α-32 P]dATP (3000 Ci mmol–1 ; Amersham) and AMV reverse transcriptase (Roche).

    Techniques: Sequencing, Binding Assay, Footprinting, Labeling

    Gel electrophoresis on RT-PCR amplification of a fragment from AMV genome using specific primer pair designed to amplify 700 bp fragment of CP gene. Lane M represents Hyper-Laddert II (Bioline), Lane 1–4 (Sample 1–4 from infected alfalfa plants collected from Sajer location), Lane 6–11 (Leaf sample from symptomatic alfalfa plants collected from Wadi aldawasser location), and Lane 5 (healthy alfalfa as a negative control.

    Journal: The Plant Pathology Journal

    Article Title: Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

    doi: 10.5423/PPJ.OA.05.2013.0050

    Figure Lengend Snippet: Gel electrophoresis on RT-PCR amplification of a fragment from AMV genome using specific primer pair designed to amplify 700 bp fragment of CP gene. Lane M represents Hyper-Laddert II (Bioline), Lane 1–4 (Sample 1–4 from infected alfalfa plants collected from Sajer location), Lane 6–11 (Leaf sample from symptomatic alfalfa plants collected from Wadi aldawasser location), and Lane 5 (healthy alfalfa as a negative control.

    Article Snippet: Molecular hybridization for detection of AMV The probe was synthesized by using PCR DIG Probe Synthesis Kit (Roche) through PCR amplification.

    Techniques: Nucleic Acid Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Amplification, Infection, Negative Control

    Results of dot blot hybridization assay of several infected alfalfa samples collected from Wadi aldawasser (Row A:1, 2, 3, 4 and 5) and Sajer (Row B: 1, 2, 3, 4 and 5) using AMV DIG-cDNA probe. (Row C:1, 2),RT-PCR product DNA as positive control. (No hybridization reaction was observed healthy alfalfa samples (Row C: 3, 4 and 5).

    Journal: The Plant Pathology Journal

    Article Title: Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

    doi: 10.5423/PPJ.OA.05.2013.0050

    Figure Lengend Snippet: Results of dot blot hybridization assay of several infected alfalfa samples collected from Wadi aldawasser (Row A:1, 2, 3, 4 and 5) and Sajer (Row B: 1, 2, 3, 4 and 5) using AMV DIG-cDNA probe. (Row C:1, 2),RT-PCR product DNA as positive control. (No hybridization reaction was observed healthy alfalfa samples (Row C: 3, 4 and 5).

    Article Snippet: Molecular hybridization for detection of AMV The probe was synthesized by using PCR DIG Probe Synthesis Kit (Roche) through PCR amplification.

    Techniques: Dot Blot, Hybridization, Infection, Reverse Transcription Polymerase Chain Reaction, Positive Control

    RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. cDNA was generated from mRNA by using the oligonucleotide SalRterm. (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).

    Journal: Journal of Bacteriology

    Article Title: Intra- and Interspecies Signaling between Streptococcus salivarius and Streptococcus pyogenes Mediated by SalA and SalA1 Lantibiotic Peptides

    doi: 10.1128/JB.183.13.3931-3938.2001

    Figure Lengend Snippet: RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. cDNA was generated from mRNA by using the oligonucleotide SalRterm. (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).

    Article Snippet: For reverse transcription (RT)-PCR analysis, DNase-treated RNA that had been extracted from exponentially growing cells was utilized for cDNA synthesis by using the SalRterm oligonuclotide primer and avian myeloblastosis virus reverse transcriptase (First Strand cDNA synthesis kit; Roche Diagnostics).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Generated

    Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription

    Journal:

    Article Title: Regulation of the Salmonella enterica std Fimbrial Operon by DNA Adenine Methylation, SeqA, and HdfR ▿

    doi: 10.1128/JB.01136-08

    Figure Lengend Snippet: Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription

    Article Snippet: The end-labeled primer was extended with avian myeloblastosis virus reverse transcriptase (Roche, Basel, Switzerland) under the conditions described by Camacho et al. ( ).

    Techniques: Sequencing