avian myeloblastosis virus reverse transcriptase  (Roche)

 
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    Structured Review

    Roche avian myeloblastosis virus reverse transcriptase
    Extension of an std mRNA primer with avian <t>myeloblastosis</t> virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Roche
    Average 92 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

    Images

    1) Product Images from "Regulation of the Salmonella enterica std Fimbrial Operon by DNA Adenine Methylation, SeqA, and HdfR ▿"

    Article Title: Regulation of the Salmonella enterica std Fimbrial Operon by DNA Adenine Methylation, SeqA, and HdfR ▿

    Journal:

    doi: 10.1128/JB.01136-08

    Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription
    Figure Legend Snippet: Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription

    Techniques Used: Sequencing

    2) Product Images from "An Arc of Unpaired "Hinge Bases" Facilitates Information Exchange among Functional Centers of the Ribosome ▿"

    Article Title: An Arc of Unpaired "Hinge Bases" Facilitates Information Exchange among Functional Centers of the Ribosome ▿

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.01311-06

    rRNA structural analyses. Sequencing ladders and primer extensions were generated with avian myeloblastosis virus reverse transcriptase using rRNAs isolated from ribosomes. Un, untreated samples; DMS, ribosomes treated in vitro with DMS; Ket, ribosomes treated in vitro with kethoxal; CMCT, ribosomes treated in vitro with CMCT. (a) Direct sequencing of the distal tip of helix 38 from rRNAs extracted from cells expressing the wild-type, G1020C, or AAA(B1a)CCC alleles. (b) Structure probing of helix 38. Individual nucleotide positions of bases in mutant samples showing enhanced in line rRNA cleavage are indicated by arrows. The asterisk indicates a strong stop at nucleotide 1021 in the G1020C mutation. (c) Time course in-line cleavage experiments. Equal amounts of freshly prepared ribosomes purified from isogenic wild-type and mutant cells were incubated at 30°C in buffer without chemical modifying agents for 0, 3, 6, and 12 h, after which rRNAs were extracted, used for reverse transcriptase primer extension with the primer described above, and separated through an 8% acrylamide-urea gel. (d) Structure probing of helix 41. The asterisks indicate enhanced in-line cleavage at A1178 specific to the G1020C mutant. (e) Structure probing along helix 91. Asterisks indicate enhanced in line cleavage of bases in the AAA(B1a)CCC mutant.
    Figure Legend Snippet: rRNA structural analyses. Sequencing ladders and primer extensions were generated with avian myeloblastosis virus reverse transcriptase using rRNAs isolated from ribosomes. Un, untreated samples; DMS, ribosomes treated in vitro with DMS; Ket, ribosomes treated in vitro with kethoxal; CMCT, ribosomes treated in vitro with CMCT. (a) Direct sequencing of the distal tip of helix 38 from rRNAs extracted from cells expressing the wild-type, G1020C, or AAA(B1a)CCC alleles. (b) Structure probing of helix 38. Individual nucleotide positions of bases in mutant samples showing enhanced in line rRNA cleavage are indicated by arrows. The asterisk indicates a strong stop at nucleotide 1021 in the G1020C mutation. (c) Time course in-line cleavage experiments. Equal amounts of freshly prepared ribosomes purified from isogenic wild-type and mutant cells were incubated at 30°C in buffer without chemical modifying agents for 0, 3, 6, and 12 h, after which rRNAs were extracted, used for reverse transcriptase primer extension with the primer described above, and separated through an 8% acrylamide-urea gel. (d) Structure probing of helix 41. The asterisks indicate enhanced in-line cleavage at A1178 specific to the G1020C mutant. (e) Structure probing along helix 91. Asterisks indicate enhanced in line cleavage of bases in the AAA(B1a)CCC mutant.

    Techniques Used: Sequencing, Generated, Isolation, In Vitro, Expressing, Mutagenesis, Purification, Incubation

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Hyperphosphorylation of the Rotavirus NSP5 Protein Is Independent of Serine 67 or NSP2, and the Intrinsic Insolubility of NSP5 Is Regulated by Cellular Phosphatases
    Article Snippet: .. At 6 h postinfection, total RNA was extracted using RNeasy (QIAGEN). cDNA was obtained by reverse transcription using random hexamers and 20 units avian myeloblastosis virus reverse transcriptase (Roche) in the presence of 1 mM deoxynucleoside triphosphates and 5 mM MgCl2 at 25°C for 10 min, 42°C for 1 h, and 94°C for 5 min. PCR was carried out using specific primers containing the 5′ and 3′ restriction site adaptors as well as N-terminal His6 G or C-terminal His6 -Myc tags (Table ). .. PCR products representing the entire open reading frame of RRV NSP5 were cloned into pcDNA3.1(+) (Invitrogen) as an N-terminally tagged 6x-His-Gly fusion protein (N-NSP5) or as a C-terminally tagged His6 G-Myc (C-NSP5) fusion protein into the EcoRI and XhoI sites.

    Random Hexamer Labeling:

    Article Title: Pseudomonas aeruginosa Rugose Small-Colony Variants Have Adaptations That Likely Promote Persistence in the Cystic Fibrosis Lung ▿ Rugose Small-Colony Variants Have Adaptations That Likely Promote Persistence in the Cystic Fibrosis Lung ▿ †
    Article Snippet: .. Duplicate cDNA synthesis reactions were performed with random hexamer primers and avian myeloblastosis virus reverse transcriptase (Roche, Germany). .. Four micrograms of RNA was mixed with 2 μl of primer (10 μM) and nuclease-free water (Ambion, Austin, TX) to obtain a final volume of 10 μl.

    Incubation:

    Article Title: Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in Photorhabdus luminescens
    Article Snippet: .. RNasin (20 U; Promega) was added, and the reaction was performed with 40 U of avian myeloblastosis virus reverse transcriptase (Roche) at 42°C for 90 min. One microliter of 0.5 M EDTA (pH 8) and 1 μl of DNase-free pancreatic RNase (Roche) were added, and the reaction mixture was further incubated at 37°C for 30 min. ..

    Synthesized:

    Article Title: Silencing of GATA3 defines a novel stem cell-like subgroup of ETP-ALL
    Article Snippet: .. Complementary DNA (cDNA) was synthesized using 500 ng of total RNA and avian myeloblastosis virus reverse transcriptase (RT-AMV; Roche, Mannheim, Germany) in the presence of RNase inhibitor (RNasin; Roche, Mannheim, Germany). .. Samples of patients with ETP-ALL (n = 70) and non-ETP-ALL (n = 112) were investigated by comparative multiplex real-time PCR (RT-PCR) for expression of GATA3 (FWD: 5′-ACTACGGAAACTCGGTCAG-3′, REV: 5′-GTAGGGATCCATGAAGCAG-3′, Probe: 5′-CGGTGCAGAGGTACCCTCCG-3′) and glucose -6 -phosphate isomerase (GPI ) as a housekeeping gene.

    Article Title: The Pepper Extracellular Xyloglucan-Specific Endo-\u03b2-1,4-Glucanase Inhibitor Protein Gene, CaXEGIP1, Is Required for Plant Cell Death and Defense Responses
    Article Snippet: .. Pepper and Arabidopsis was synthesized from total RNA (2 μg) using avian myeloblastosis virus reverse transcriptase (Roche) with oligo(dT)15 as a primer (Roche) according to the manufacturer’s instructions. .. -PCR was performed using ExTaq polymerase (Takara Biomedicals).

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    Roche amv reverse transcriptase
    Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with <t>[α-</t> 32 P]dATP in the presence of <t>AMV</t> reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.
    Amv Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase/product/Roche
    Average 92 stars, based on 140 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Roche first strand cdna synthesis kit
    RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. <t>cDNA</t> was generated from mRNA by using the oligonucleotide <t>SalRterm.</t> (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).
    First Strand Cdna Synthesis Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand cdna synthesis kit/product/Roche
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    first strand cdna synthesis kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Roche avian myeloblastosis virus reverse transcriptase
    Extension of an std mRNA primer with avian <t>myeloblastosis</t> virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Roche
    Average 92 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with [α- 32 P]dATP in the presence of AMV reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.

    Journal: Nucleic Acids Research

    Article Title: DNA sequence recognition by the indolocarbazole antitumor antibiotic AT2433-B1 and its diastereoisomer

    doi:

    Figure Lengend Snippet: Sequence-selective binding. DNase I footprinting gel obtained with the 117 bp Pvu II– Eco RI restriction fragment in the presence of graded concentrations of AT2433-B1 and iso-AT2433-B1. The DNA was 3′-end-labeled at the Eco RI site with [α- 32 P]dATP in the presence of AMV reverse transcriptase. The products of nuclease digestion were resolved on an 8% polyacrylamide gel containing 7 M urea. Control tracks (Cont) contained no drug. Guanine-specific sequence markers obtained by treatment of the DNA with dimethylsulfate followed by piperidine were run in the lane marked G. Numbers on the left side of the gel refer to the standard numbering scheme for the nucleotide sequence of the DNA fragment, as indicated in Figure 3.

    Article Snippet: The 117 bp DNA fragment was prepared by 3′-32 P-end-labeling of an Eco RI + Pvu II double digest of plasmid pBS (Stratagene) using [α-32 P]dATP (3000 Ci mmol–1 ; Amersham) and AMV reverse transcriptase (Roche).

    Techniques: Sequencing, Binding Assay, Footprinting, Labeling

    RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. cDNA was generated from mRNA by using the oligonucleotide SalRterm. (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).

    Journal: Journal of Bacteriology

    Article Title: Intra- and Interspecies Signaling between Streptococcus salivarius and Streptococcus pyogenes Mediated by SalA and SalA1 Lantibiotic Peptides

    doi: 10.1128/JB.183.13.3931-3938.2001

    Figure Lengend Snippet: RT-PCR analysis of sal locus transcripts from S. salivarius 20P3. cDNA was generated from mRNA by using the oligonucleotide SalRterm. (A) Lanes 2 to 5 PCRs performed with primers SalAF and SalXR; lanes 7 to 10, PCRs performed with primers SalY2S and SalRterm; lanes 2 and 7, cDNA template generated by RT; lanes 3 and 8, RNA controls (no RT); lanes 4 and 9, chromosomal DNA template; lanes 5 and 10, no-template controls. (B) PCRs performed with primers SalAF and SalRR. Lane 2, cDNA template generated by RT; lane 3, RNA control; lane 4, chromosomal DNA; lane 5, no template. The molecular mass markers (panel A, lanes 1 and 6; panel B, lane 1) were λ DNA Hin dIII fragments (23.1, 9.41, 6.56, 4.36, 2.32, and 2.02 kb).

    Article Snippet: For reverse transcription (RT)-PCR analysis, DNase-treated RNA that had been extracted from exponentially growing cells was utilized for cDNA synthesis by using the SalRterm oligonuclotide primer and avian myeloblastosis virus reverse transcriptase (First Strand cDNA synthesis kit; Roche Diagnostics).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Generated

    Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription

    Journal:

    Article Title: Regulation of the Salmonella enterica std Fimbrial Operon by DNA Adenine Methylation, SeqA, and HdfR ▿

    doi: 10.1128/JB.01136-08

    Figure Lengend Snippet: Extension of an std mRNA primer with avian myeloblastosis virus reverse transcriptase. The extension product is indicated by an arrow. The DNA sequence of the std promoter region, the putative −10 and −35 modules, and the transcription

    Article Snippet: The end-labeled primer was extended with avian myeloblastosis virus reverse transcriptase (Roche, Basel, Switzerland) under the conditions described by Camacho et al. ( ).

    Techniques: Sequencing

    rRNA structural analyses. Sequencing ladders and primer extensions were generated with avian myeloblastosis virus reverse transcriptase using rRNAs isolated from ribosomes. Un, untreated samples; DMS, ribosomes treated in vitro with DMS; Ket, ribosomes treated in vitro with kethoxal; CMCT, ribosomes treated in vitro with CMCT. (a) Direct sequencing of the distal tip of helix 38 from rRNAs extracted from cells expressing the wild-type, G1020C, or AAA(B1a)CCC alleles. (b) Structure probing of helix 38. Individual nucleotide positions of bases in mutant samples showing enhanced in line rRNA cleavage are indicated by arrows. The asterisk indicates a strong stop at nucleotide 1021 in the G1020C mutation. (c) Time course in-line cleavage experiments. Equal amounts of freshly prepared ribosomes purified from isogenic wild-type and mutant cells were incubated at 30°C in buffer without chemical modifying agents for 0, 3, 6, and 12 h, after which rRNAs were extracted, used for reverse transcriptase primer extension with the primer described above, and separated through an 8% acrylamide-urea gel. (d) Structure probing of helix 41. The asterisks indicate enhanced in-line cleavage at A1178 specific to the G1020C mutant. (e) Structure probing along helix 91. Asterisks indicate enhanced in line cleavage of bases in the AAA(B1a)CCC mutant.

    Journal: Molecular and Cellular Biology

    Article Title: An Arc of Unpaired "Hinge Bases" Facilitates Information Exchange among Functional Centers of the Ribosome ▿

    doi: 10.1128/MCB.01311-06

    Figure Lengend Snippet: rRNA structural analyses. Sequencing ladders and primer extensions were generated with avian myeloblastosis virus reverse transcriptase using rRNAs isolated from ribosomes. Un, untreated samples; DMS, ribosomes treated in vitro with DMS; Ket, ribosomes treated in vitro with kethoxal; CMCT, ribosomes treated in vitro with CMCT. (a) Direct sequencing of the distal tip of helix 38 from rRNAs extracted from cells expressing the wild-type, G1020C, or AAA(B1a)CCC alleles. (b) Structure probing of helix 38. Individual nucleotide positions of bases in mutant samples showing enhanced in line rRNA cleavage are indicated by arrows. The asterisk indicates a strong stop at nucleotide 1021 in the G1020C mutation. (c) Time course in-line cleavage experiments. Equal amounts of freshly prepared ribosomes purified from isogenic wild-type and mutant cells were incubated at 30°C in buffer without chemical modifying agents for 0, 3, 6, and 12 h, after which rRNAs were extracted, used for reverse transcriptase primer extension with the primer described above, and separated through an 8% acrylamide-urea gel. (d) Structure probing of helix 41. The asterisks indicate enhanced in-line cleavage at A1178 specific to the G1020C mutant. (e) Structure probing along helix 91. Asterisks indicate enhanced in line cleavage of bases in the AAA(B1a)CCC mutant.

    Article Snippet: [γ-32 P]ATP (Perkin-Elmer, Wellesley, MA) T4 polynucleotide kinase, and avian myeloblastosis virus reverse transcriptase (Roche) were used for primer extension.

    Techniques: Sequencing, Generated, Isolation, In Vitro, Expressing, Mutagenesis, Purification, Incubation