Structured Review

Boehringer Mannheim avian myeloblastosis virus reverse transcriptase
Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Boehringer Mannheim
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
92/100 stars

Images

1) Product Images from "Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease"

Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
Figure Legend Snippet: Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).

Techniques Used: Transgenic Assay, Expressing, Polymerase Chain Reaction, Mouse Assay, Marker, Immunoprecipitation, Western Blot

Related Articles

Polymerase Chain Reaction:

Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease
Article Snippet: .. Five micrograms of tissue RNA extracted with guanidium isothiocyanate/phenol (RNAzol, Cinna/Biotecx, Houston, TX) were primed with random hexamers, reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), and equal amounts (quantified by spectrophotometry) amplified by PCR. .. In the case of islets, cDNA was quantified by PCR titration, using hypoxanthine-guanine phosphoribosyltransferase (HPRT) primers.

Generated:

Article Title: Enhanced Epidermal Growth Factor, Hepatocyte Growth Factor, and Vascular Endothelial Growth Factor Expression in Tuberous Sclerosis Complex
Article Snippet: .. Amplified mRNA served as a template for a second round of cDNA synthesis with avian myeloblastosis virus reverse transcriptase, deoxynucleotide triphosphates, and N6 random hexamers (Boehringer-Mannheim). cDNA generated from amplified mRNA was made double stranded and served as template for a second mRNA amplification, again incorporating 32 P CTP radiolabel. .. The radiolabeled, amplified mRNA was used as a probe for cDNA arrays.

Amplification:

Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease
Article Snippet: .. Five micrograms of tissue RNA extracted with guanidium isothiocyanate/phenol (RNAzol, Cinna/Biotecx, Houston, TX) were primed with random hexamers, reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), and equal amounts (quantified by spectrophotometry) amplified by PCR. .. In the case of islets, cDNA was quantified by PCR titration, using hypoxanthine-guanine phosphoribosyltransferase (HPRT) primers.

Article Title: Enhanced Epidermal Growth Factor, Hepatocyte Growth Factor, and Vascular Endothelial Growth Factor Expression in Tuberous Sclerosis Complex
Article Snippet: .. Amplified mRNA served as a template for a second round of cDNA synthesis with avian myeloblastosis virus reverse transcriptase, deoxynucleotide triphosphates, and N6 random hexamers (Boehringer-Mannheim). cDNA generated from amplified mRNA was made double stranded and served as template for a second mRNA amplification, again incorporating 32 P CTP radiolabel. .. The radiolabeled, amplified mRNA was used as a probe for cDNA arrays.

Incubation:

Article Title: Transcriptional Analysis of the sfa Determinant Revealing Multiple mRNA Processing Events in the Biogenesis of S Fimbriae in Pathogenic Escherichia coli
Article Snippet: .. The primer extension reactions were performed by using 1 h of incubation at 42°C after addition of 8 μl of extension buffer containing 1 U of avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), 25 mM Tris-HCl (pH 8.3), 30 mM NaCl, 15 mM MgCl2 , 1.25 mM dithiothreitol, 1 mM dATP, 1 mM dCTP, 1 mM dGTP, and 1 mM dTTP. ..

Spectrophotometry:

Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease
Article Snippet: .. Five micrograms of tissue RNA extracted with guanidium isothiocyanate/phenol (RNAzol, Cinna/Biotecx, Houston, TX) were primed with random hexamers, reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), and equal amounts (quantified by spectrophotometry) amplified by PCR. .. In the case of islets, cDNA was quantified by PCR titration, using hypoxanthine-guanine phosphoribosyltransferase (HPRT) primers.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Boehringer Mannheim avian myeloblastosis virus reverse transcriptase
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Boehringer Mannheim
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    88
    Boehringer Mannheim amv reverse transcriptase rt
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Amv Reverse Transcriptase Rt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase rt/product/Boehringer Mannheim
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase rt - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease

    doi:

    Figure Lengend Snippet: Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).

    Article Snippet: Five micrograms of tissue RNA extracted with guanidium isothiocyanate/phenol (RNAzol, Cinna/Biotecx, Houston, TX) were primed with random hexamers, reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), and equal amounts (quantified by spectrophotometry) amplified by PCR.

    Techniques: Transgenic Assay, Expressing, Polymerase Chain Reaction, Mouse Assay, Marker, Immunoprecipitation, Western Blot