avian myeloblastosis virus reverse transcriptase  (Bio-Rad)

 
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    Bio-Rad avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transcriptase/product/Bio-Rad
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transcriptase - by Bioz Stars, 2020-08
    91/100 stars

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    Isolation:

    Article Title: B-1a Cell Diversity: N-addition in B-1a immunoglobulin is determined by progenitor population and developmental location
    Article Snippet: .. Following isolation RNA was treated with DNaseI (Ambion) to remove contaminating DNA. cDNA was prepared using avian myeloblastosis virus reverse transcriptase (Bio-Rad). .. For TdT expression analysis following culture RNA was prepared from 10,000-100,000 cells (Cells-to-Ct kit, Ambion).

    other:

    Article Title: CD25+ B-1a Cells Express Aicda
    Article Snippet: Briefly, RNA was prepared from B cells using the RNeasy mini kit (Qiagen), according to the manufacturer’s instructions. cDNA was prepared using avian myeloblastosis virus reverse transcriptase (Bio-Rad).

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    Bio-Rad amv reverse transcriptase
    Development of <t>RT-AIOD-CRISPR</t> assay. (A) Protocol and RT-PCR primers for preparing HIV-1 gag RNA sequences. (B) Sanger sequencing of RT-AIO-Cas12aR-based detection region in the prepared HIV-1 gag RNA. (C) The RT-AIOD-CRISPR assay using various concentrations of <t>AMV</t> reverse transcriptase. Three replicates ran for each reaction or test. P, the positive reaction with 1.1× 10 3 copies of HIV-1 gag RNA sequences. NTC, non-target control reaction.
    Amv Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase/product/Bio-Rad
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amv reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
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    Development of RT-AIOD-CRISPR assay. (A) Protocol and RT-PCR primers for preparing HIV-1 gag RNA sequences. (B) Sanger sequencing of RT-AIO-Cas12aR-based detection region in the prepared HIV-1 gag RNA. (C) The RT-AIOD-CRISPR assay using various concentrations of AMV reverse transcriptase. Three replicates ran for each reaction or test. P, the positive reaction with 1.1× 10 3 copies of HIV-1 gag RNA sequences. NTC, non-target control reaction.

    Journal: bioRxiv

    Article Title: All-in-One Dual CRISPR-Cas12a (AIOD-CRISPR) Assay: A Case for Rapid, Ultrasensitive and Visual Detection of Novel Coronavirus SARS-CoV-2 and HIV virus

    doi: 10.1101/2020.03.19.998724

    Figure Lengend Snippet: Development of RT-AIOD-CRISPR assay. (A) Protocol and RT-PCR primers for preparing HIV-1 gag RNA sequences. (B) Sanger sequencing of RT-AIO-Cas12aR-based detection region in the prepared HIV-1 gag RNA. (C) The RT-AIOD-CRISPR assay using various concentrations of AMV reverse transcriptase. Three replicates ran for each reaction or test. P, the positive reaction with 1.1× 10 3 copies of HIV-1 gag RNA sequences. NTC, non-target control reaction.

    Article Snippet: For RT-AIOD-CRISPR assays, most components were the same as those in the AIOD-CRISPR system above, except supplementing 0.32 U/μL AMV Reverse Transcriptase in Component A. Real-time fluorescence detection was carried out in the Bio-Rad CFX96 Touch™ Real-Time PCR Detection System.

    Techniques: CRISPR, Reverse Transcription Polymerase Chain Reaction, Sequencing