avian myeloblastosis virus reverse transciptase  (TaKaRa)

 
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    Structured Review

    TaKaRa avian myeloblastosis virus reverse transciptase
    Avian Myeloblastosis Virus Reverse Transciptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse transciptase/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse transciptase - by Bioz Stars, 2020-08
    85/100 stars

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    Synthesized:

    Article Title: Study of the correlation between H-ras mutation and primary hepatocellular carcinoma
    Article Snippet: .. First-strand cDNA was synthesized using 5 μ l total RNA with oligo (dT) 16 primer in a 50- μ l reverse transcription mixture containing 10 μ l of 5X first strand buffer, 2.5 μ l dNTP mixture containing 25 mmol/l of each deoxynucleotide triphosphate base (Pharmacia Biotech Co., Tokyo, Japan), 2.5 μ l ribonuclease inhibitor (Takara Biochemicals, Ohotsu, Japan), 25 μ l ddH2 O (managed with DEPC in advance) and 2.5 μ l avian myeloblastosis virus reverse transciptase (Takara Biochemicals). .. The resulting cDNA was used for PCR amplification using Taq polymerse (Takara Biochemicals).

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    TaKaRa amv reverse transcriptase rtase xl
    Transcriptional expression of the ggh genes. A. Dot blot analysis using the meningococcal ggt gene as a probe. One to 16 micrograms of RNAs isolated from H44/76, HT1089 (H44/76 Δggt::spc ) [39], ATCC49226, NIID54, NIID103 and NIID106 were subjected to this analysis. B. RT-PCR to detect the transcripts of the gonococcal ggh genes. The schematic figure in the box depicts the position of the primers used in this experiment (see Table 1). RT-PCR was performed without reverse transcriptase <t>(RTase)</t> (lanes 1 to 5) or with RTase (lanes 6 to 10). Lanes 1 and 6, H44/76 ( N. meningitidis ); lanes 2 and 7, ATCC49226 ( N. gonorrhoeae ); lanes 3 and 8, NIID54 ( N. gonorrhoeae ); lanes 4 and 9, NIID103 ( N. gonorrhoeae ); lanes 5 and 10, NIID106 ( N. gonorrhoeae ). The marker in the left-most lane is φ X174 DNA digested with Hae III. Primer sets used for RT-PCR are shown on the left side, and the corresponding PCR products are indicated by arrows on the right side. C. Primer extension analysis to detect the transcriptional start point of the ggt and ggh genes. Total RNA extracted from H44/76 ( N. meningitidis ), ATCC49226 and NIID106 ( N. gonorrhoeae ) was used for the primer extension with <t>AMV</t> reverse transcriptase XL and biotin-labeled oligonucleotide ggt-ext-2. The arrow on the right side indicates the transcriptional start site. D. Alignment of the nucleotide sequences of the upstream regions of the ggt and ggh genes. The sequence data have been deposited in the DDBJ/EMBL/GenBank Databases under the following Accession Numbers: N. meningitidis strains H44/76 [DDBJ: AB193252 ], H114/90 [DDBJ: AB193253 ], N. gonorrhoeae strains ATCC49226 [DDBJ: AB193254 ], NIID54 [DDBJ: AB193255 ], NIID103 [DDBJ: AB193296 ], NIID106 [DDBJ: AB193256 ]. An identical nucleotide is represented as *. The transcriptional start site is shown in bold as +1. The putative -35, -10 elements and Shine-Dalgarno sequence (SD) are depicted in the box, and the ideal -35 and -10 nucleotide sequences are shown above the boxes. The previously predicted start codon (ATG) [25] and newly predicted start codon (GTG) of the meningococcal {\it ggt} gene are underlined. The amino acid sequence deduced from the putative start codon GTG (shown in bold) in the meningococcal {\it ggt} gene is also shown under the corresponding nucleotide sequences.
    Amv Reverse Transcriptase Rtase Xl, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv reverse transcriptase rtase xl/product/TaKaRa
    Average 99 stars, based on 219 article reviews
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    TaKaRa rna pcr kit
    Transcription profiles of the xylanase genes in water-insoluble xylan medium. <t>RT-PCR</t> analysis was performed on total <t>RNA,</t> isolated at the times indicated, from the PW5001 mutant (A) and strain W-61 (B) cells incubated at 30°C in water-insoluble
    Rna Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 607 article reviews
    Price from $9.99 to $1999.99
    rna pcr kit - by Bioz Stars, 2020-08
    99/100 stars
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    Transcriptional expression of the ggh genes. A. Dot blot analysis using the meningococcal ggt gene as a probe. One to 16 micrograms of RNAs isolated from H44/76, HT1089 (H44/76 Δggt::spc ) [39], ATCC49226, NIID54, NIID103 and NIID106 were subjected to this analysis. B. RT-PCR to detect the transcripts of the gonococcal ggh genes. The schematic figure in the box depicts the position of the primers used in this experiment (see Table 1). RT-PCR was performed without reverse transcriptase (RTase) (lanes 1 to 5) or with RTase (lanes 6 to 10). Lanes 1 and 6, H44/76 ( N. meningitidis ); lanes 2 and 7, ATCC49226 ( N. gonorrhoeae ); lanes 3 and 8, NIID54 ( N. gonorrhoeae ); lanes 4 and 9, NIID103 ( N. gonorrhoeae ); lanes 5 and 10, NIID106 ( N. gonorrhoeae ). The marker in the left-most lane is φ X174 DNA digested with Hae III. Primer sets used for RT-PCR are shown on the left side, and the corresponding PCR products are indicated by arrows on the right side. C. Primer extension analysis to detect the transcriptional start point of the ggt and ggh genes. Total RNA extracted from H44/76 ( N. meningitidis ), ATCC49226 and NIID106 ( N. gonorrhoeae ) was used for the primer extension with AMV reverse transcriptase XL and biotin-labeled oligonucleotide ggt-ext-2. The arrow on the right side indicates the transcriptional start site. D. Alignment of the nucleotide sequences of the upstream regions of the ggt and ggh genes. The sequence data have been deposited in the DDBJ/EMBL/GenBank Databases under the following Accession Numbers: N. meningitidis strains H44/76 [DDBJ: AB193252 ], H114/90 [DDBJ: AB193253 ], N. gonorrhoeae strains ATCC49226 [DDBJ: AB193254 ], NIID54 [DDBJ: AB193255 ], NIID103 [DDBJ: AB193296 ], NIID106 [DDBJ: AB193256 ]. An identical nucleotide is represented as *. The transcriptional start site is shown in bold as +1. The putative -35, -10 elements and Shine-Dalgarno sequence (SD) are depicted in the box, and the ideal -35 and -10 nucleotide sequences are shown above the boxes. The previously predicted start codon (ATG) [25] and newly predicted start codon (GTG) of the meningococcal {\it ggt} gene are underlined. The amino acid sequence deduced from the putative start codon GTG (shown in bold) in the meningococcal {\it ggt} gene is also shown under the corresponding nucleotide sequences.

    Journal: BMC Microbiology

    Article Title: A gonococcal homologue of meningococcal ?-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent

    doi: 10.1186/1471-2180-5-56

    Figure Lengend Snippet: Transcriptional expression of the ggh genes. A. Dot blot analysis using the meningococcal ggt gene as a probe. One to 16 micrograms of RNAs isolated from H44/76, HT1089 (H44/76 Δggt::spc ) [39], ATCC49226, NIID54, NIID103 and NIID106 were subjected to this analysis. B. RT-PCR to detect the transcripts of the gonococcal ggh genes. The schematic figure in the box depicts the position of the primers used in this experiment (see Table 1). RT-PCR was performed without reverse transcriptase (RTase) (lanes 1 to 5) or with RTase (lanes 6 to 10). Lanes 1 and 6, H44/76 ( N. meningitidis ); lanes 2 and 7, ATCC49226 ( N. gonorrhoeae ); lanes 3 and 8, NIID54 ( N. gonorrhoeae ); lanes 4 and 9, NIID103 ( N. gonorrhoeae ); lanes 5 and 10, NIID106 ( N. gonorrhoeae ). The marker in the left-most lane is φ X174 DNA digested with Hae III. Primer sets used for RT-PCR are shown on the left side, and the corresponding PCR products are indicated by arrows on the right side. C. Primer extension analysis to detect the transcriptional start point of the ggt and ggh genes. Total RNA extracted from H44/76 ( N. meningitidis ), ATCC49226 and NIID106 ( N. gonorrhoeae ) was used for the primer extension with AMV reverse transcriptase XL and biotin-labeled oligonucleotide ggt-ext-2. The arrow on the right side indicates the transcriptional start site. D. Alignment of the nucleotide sequences of the upstream regions of the ggt and ggh genes. The sequence data have been deposited in the DDBJ/EMBL/GenBank Databases under the following Accession Numbers: N. meningitidis strains H44/76 [DDBJ: AB193252 ], H114/90 [DDBJ: AB193253 ], N. gonorrhoeae strains ATCC49226 [DDBJ: AB193254 ], NIID54 [DDBJ: AB193255 ], NIID103 [DDBJ: AB193296 ], NIID106 [DDBJ: AB193256 ]. An identical nucleotide is represented as *. The transcriptional start site is shown in bold as +1. The putative -35, -10 elements and Shine-Dalgarno sequence (SD) are depicted in the box, and the ideal -35 and -10 nucleotide sequences are shown above the boxes. The previously predicted start codon (ATG) [25] and newly predicted start codon (GTG) of the meningococcal {\it ggt} gene are underlined. The amino acid sequence deduced from the putative start codon GTG (shown in bold) in the meningococcal {\it ggt} gene is also shown under the corresponding nucleotide sequences.

    Article Snippet: The hybridized RNA-DNA probe was treated with 35 units of AMV reverse transcriptase (RTase) XL (Takara Bio) in a reaction mixture (250 μM dNTPs, 1 × AMV reverse transcriptase XL buffer) at 37°C for 30 min.

    Techniques: Expressing, Dot Blot, Isolation, Reverse Transcription Polymerase Chain Reaction, Marker, Polymerase Chain Reaction, Labeling, Sequencing

    ( A ) Sequences of the 25 nt template ( III ) and 20 nt primer ( IV ). ( B ) PAGE of extension reactions beyond the 1-deaza-dG residue in the template. ( C ) PAGE of extension reactions subsequent to 1-deaza-dG in the primer with Therminator™ DNA polymerase and ( D ) with AMV reverse transcriptase. Conditions used are described in Materials and Methods. Added dNTPs are indicated at the top of each gel. Z indicates 1-deaza-dGTP; P indicates reaction without dNTP.

    Journal: Nucleic Acids Research

    Article Title: A new, but old, nucleoside analog: the first synthesis of 1-deaza-2?-deoxyguanosine and its properties as a nucleoside and as oligodeoxynucleotides

    doi: 10.1093/nar/gkh154

    Figure Lengend Snippet: ( A ) Sequences of the 25 nt template ( III ) and 20 nt primer ( IV ). ( B ) PAGE of extension reactions beyond the 1-deaza-dG residue in the template. ( C ) PAGE of extension reactions subsequent to 1-deaza-dG in the primer with Therminator™ DNA polymerase and ( D ) with AMV reverse transcriptase. Conditions used are described in Materials and Methods. Added dNTPs are indicated at the top of each gel. Z indicates 1-deaza-dGTP; P indicates reaction without dNTP.

    Article Snippet: AMV reverse transcriptase, M-MLV reverse transcriptase and dNTPs were purchased from Takara Biomedicals (Japan).

    Techniques: Polyacrylamide Gel Electrophoresis

    PAGE of single nucleotide insertion studies ( A ) with Therminator™ DNA polymerase (reaction mixtures being incubated at 74°C for 10 min), ( B ) with AMV reverse transcriptase (reaction mixtures being incubated at 37°C for 30 min) and ( C ) with M-MLV reverse transcriptase (reactions mixture being incubated at 37°C for 30 min). Conditions used are described in Materials and Methods. Both X in the template sequence and added dNTP are indicated at the top of each gel. Z indicates 1-deaza-dGTP; P indicates reaction without dNTP.

    Journal: Nucleic Acids Research

    Article Title: A new, but old, nucleoside analog: the first synthesis of 1-deaza-2?-deoxyguanosine and its properties as a nucleoside and as oligodeoxynucleotides

    doi: 10.1093/nar/gkh154

    Figure Lengend Snippet: PAGE of single nucleotide insertion studies ( A ) with Therminator™ DNA polymerase (reaction mixtures being incubated at 74°C for 10 min), ( B ) with AMV reverse transcriptase (reaction mixtures being incubated at 37°C for 30 min) and ( C ) with M-MLV reverse transcriptase (reactions mixture being incubated at 37°C for 30 min). Conditions used are described in Materials and Methods. Both X in the template sequence and added dNTP are indicated at the top of each gel. Z indicates 1-deaza-dGTP; P indicates reaction without dNTP.

    Article Snippet: AMV reverse transcriptase, M-MLV reverse transcriptase and dNTPs were purchased from Takara Biomedicals (Japan).

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation, Sequencing

    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to cDNA using AMV reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.

    Journal: BMC Molecular Biology

    Article Title: Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival

    doi: 10.1186/1471-2199-11-65

    Figure Lengend Snippet: (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to cDNA using AMV reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.

    Article Snippet: Total RNA was extracted from individual larvae using the acid guanidinium thiocyanate-phenol-chloroform method [ , ] and converted to cDNA using AMV reverse transcriptase (Takara).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Injection, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Hybridization

    Transcription profiles of the xylanase genes in water-insoluble xylan medium. RT-PCR analysis was performed on total RNA, isolated at the times indicated, from the PW5001 mutant (A) and strain W-61 (B) cells incubated at 30°C in water-insoluble

    Journal: Journal of Bacteriology

    Article Title: Cell Surface Xylanases of the Glycoside Hydrolase Family 10 Are Essential for Xylan Utilization by Paenibacillus sp. W-61 as Generators of Xylo-Oligosaccharide Inducers for the Xylanase Genes ▿

    doi: 10.1128/JB.01406-09

    Figure Lengend Snippet: Transcription profiles of the xylanase genes in water-insoluble xylan medium. RT-PCR analysis was performed on total RNA, isolated at the times indicated, from the PW5001 mutant (A) and strain W-61 (B) cells incubated at 30°C in water-insoluble

    Article Snippet: Total RNA was prepared from W-61 and Δ xyn5 mutant (PW5001) cells by the hot-phenol method described by Aiba et al. ( ). cDNAs of xyn1 , xyn3 , and xyn5 were synthesized using avian myeloblastosis virus reverse transcriptase XL (TaKaRa RNA PCR kit, version 3.6; TaKaRa Bio, Japan) in 10-μl reaction mixtures containing 100 ng of total RNA and 1.0 μM concentrations of primers P14, P15, and P16 (Table ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Mutagenesis, Incubation