avian myeloblastosis virus reverse trancriptase  (Promega)

 
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    Promega avian myeloblastosis virus reverse trancriptase
    Avian Myeloblastosis Virus Reverse Trancriptase, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian myeloblastosis virus reverse trancriptase/product/Promega
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    avian myeloblastosis virus reverse trancriptase - by Bioz Stars, 2020-08
    85/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Loss of a Conserved tRNA Anticodon Modification Perturbs Cellular Signaling
    Article Snippet: .. Reverse transcription and quantitative PCR was performed using Avian Myeloblastosis Virus Reverse Trancriptase (AMV-RT; Promega) and real-time reagents (Invitrogen) according to manufacturer's instructions using a Roche Lightcycler 480. ..

    Random Hexamer Labeling:

    Article Title: Protein kinase A regulates gene-specific translational adaptation in differentiating yeast
    Article Snippet: .. Reverse transcription was performed using SuperScript III (Invitrogen) or Avian Myeloblastosis Virus Reverse Trancriptase (AMV-RT; Promega) using a mix of oligo-dT and random hexamer primers. .. Quantitative PCR was performed with SYBR Fast reagents (Kapa Biosystems) according to the manufacturer's instructions using a Roche Lightcycler 480.

    other:

    Article Title: Chronic Herpesvirus Reactivation Occurs in Aging
    Article Snippet: To determine viral gene transcription, specimen RNA was treated with DNase and was reverse transcribed into cDNA by use of oligo-dT and EBER1-specific primers and avian myeloblastosis virus reverse trancriptase (Promega, Madison, WI).

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    Promega amv rt
    Utilization of dGDP as substrate by the <t>AMV</t> RT and T4 <t>DNA</t> polymerase Sequences of the DNA/RNA or DNA/DNA hybrid substrates are shown above the gels. The templates of the substrates specify incorporation for a dA and a dG residue. The enzymes were assayed with 0.165 μM 32 P‐dATP (asterisk) and either dGMP, dGDP, or dGTP at 100 μM.
    Amv Rt, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amv rt/product/Promega
    Average 99 stars, based on 702 article reviews
    Price from $9.99 to $1999.99
    amv rt - by Bioz Stars, 2020-08
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    Utilization of dGDP as substrate by the AMV RT and T4 DNA polymerase Sequences of the DNA/RNA or DNA/DNA hybrid substrates are shown above the gels. The templates of the substrates specify incorporation for a dA and a dG residue. The enzymes were assayed with 0.165 μM 32 P‐dATP (asterisk) and either dGMP, dGDP, or dGTP at 100 μM.

    Journal: The EMBO Journal

    Article Title: A single nucleotide incorporation step limits human telomerase repeat addition activity

    doi: 10.15252/embj.201797953

    Figure Lengend Snippet: Utilization of dGDP as substrate by the AMV RT and T4 DNA polymerase Sequences of the DNA/RNA or DNA/DNA hybrid substrates are shown above the gels. The templates of the substrates specify incorporation for a dA and a dG residue. The enzymes were assayed with 0.165 μM 32 P‐dATP (asterisk) and either dGMP, dGDP, or dGTP at 100 μM.

    Article Snippet: One microliter of RRL reconstituted telomerase enzyme, 1 unit of AMV RT (Promega), 0.5 units of Taq DNA pol III (NEB), 1 unit of T4 DNA pol (Fermentas), or 0.5 units of Klenow fragment of DNA pol I (Invitrogen) were assayed in 10 μl reactions containing 1× telomerase reaction buffer, 40 μM of denoted pre‐annealed DNA/RNA or DNA/DNA hybrid substrates, 100 μM dGTP, dGDP, or dGMP, and 0.165 μM α‐32 P‐dATP.

    Techniques:

    Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Journal: Journal of Virology

    Article Title: The Arginine Clusters of the Carboxy-Terminal Domain of the Core Protein of Hepatitis B Virus Make Pleiotropic Contributions to Genome Replication ▿

    doi: 10.1128/JVI.01957-10

    Figure Lengend Snippet: Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Article Snippet: For simultaneous detection of RNA and DNA by primer extension (as shown in Fig. and ) a cDNA of pgRNA was generated using AMV reverse transcriptase (Promega) and an unlabeled primer.

    Techniques: Sequencing

    Primer extension analysis of human brain RNA. Oligonucleotides GSP-6, PEXT1 and PEXT2 flanking the 5′ termini of the human LGA cDNA clone were 32 P end-labeled with [γ- 32 P]ATP and T4 polynucleotide kinase. Poly(A) + mRNA of human brain (approx. 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse-transcribed with avian myeloblastosis virus reverse transcriptase; the products from primer extension reactions were separated by electrophoresis on an 8% (w/v) denaturing polyacrylamide gel. Lanes are labeled with the name of the specific primer used in each case. The size of the single-stranded cDNA products (in bases) was estimated approximately by comparison with ФX174 HinfI DNA markers shown at the left in lane M. C+ lane: product amplified with a positive control of kanamycin. C- lanes: negative controls using PEXT2 with mRNA omitted. The black arrow indicates the specific GA products of approx. 140 bases obtained by reverse transcription with primer PEXT2. Top panel: scheme showing the approximate position of the three primers with regard to the TSS (+1) and the two first ATG codons of human LGA transcript.

    Journal: PLoS ONE

    Article Title: Mammalian Glutaminase Gls2 Gene Encodes Two Functional Alternative Transcripts by a Surrogate Promoter Usage Mechanism

    doi: 10.1371/journal.pone.0038380

    Figure Lengend Snippet: Primer extension analysis of human brain RNA. Oligonucleotides GSP-6, PEXT1 and PEXT2 flanking the 5′ termini of the human LGA cDNA clone were 32 P end-labeled with [γ- 32 P]ATP and T4 polynucleotide kinase. Poly(A) + mRNA of human brain (approx. 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse-transcribed with avian myeloblastosis virus reverse transcriptase; the products from primer extension reactions were separated by electrophoresis on an 8% (w/v) denaturing polyacrylamide gel. Lanes are labeled with the name of the specific primer used in each case. The size of the single-stranded cDNA products (in bases) was estimated approximately by comparison with ФX174 HinfI DNA markers shown at the left in lane M. C+ lane: product amplified with a positive control of kanamycin. C- lanes: negative controls using PEXT2 with mRNA omitted. The black arrow indicates the specific GA products of approx. 140 bases obtained by reverse transcription with primer PEXT2. Top panel: scheme showing the approximate position of the three primers with regard to the TSS (+1) and the two first ATG codons of human LGA transcript.

    Article Snippet: 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse transcribed with avian myeloblastosis virus reverse transcriptase, with the use of the protocol provided by the manufacturer (Primer Extension Analysis kit; Promega).

    Techniques: Labeling, Electrophoresis, Amplification, Positive Control

    FcRn expression in normal adult human small intestine and human intestinal epithelial cell lines. Western blots of total cellular protein (13 μg protein per lane, a ; 10 μg protein per lane, b ) isolated from the indicated source using affinity-purified rabbit antisera raised against amino acids 112–125 ( a ) or amino acids 174–188 ( b ). ( c ) RT-PCR detection of FcγRI transcripts. Total RNA (2 μg) from T84 (lanes 3 and 4), MOLT-4 (lanes 5 and 6; negative control), and U937 (lanes 1 and 2; positive control) cell lines was incubated with an oligo-dT primer with (odd-numbered lanes) or without (even-numbered lanes) avian myeloblastosis virus–RT (AMV-RT), and a nested PCR was performed with primers specific for FcγRI cDNA (top) or for β-actin (bottom).

    Journal: Journal of Clinical Investigation

    Article Title: Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line

    doi:

    Figure Lengend Snippet: FcRn expression in normal adult human small intestine and human intestinal epithelial cell lines. Western blots of total cellular protein (13 μg protein per lane, a ; 10 μg protein per lane, b ) isolated from the indicated source using affinity-purified rabbit antisera raised against amino acids 112–125 ( a ) or amino acids 174–188 ( b ). ( c ) RT-PCR detection of FcγRI transcripts. Total RNA (2 μg) from T84 (lanes 3 and 4), MOLT-4 (lanes 5 and 6; negative control), and U937 (lanes 1 and 2; positive control) cell lines was incubated with an oligo-dT primer with (odd-numbered lanes) or without (even-numbered lanes) avian myeloblastosis virus–RT (AMV-RT), and a nested PCR was performed with primers specific for FcγRI cDNA (top) or for β-actin (bottom).

    Article Snippet: RNA (2 μg) was reverse-transcribed to cDNA with an oligo-dT primer (Promega Corp., Madison, Wisconsin, USA) and avian myeloblastosis virus reverse transcriptase (Promega Corp.).

    Techniques: Expressing, Western Blot, Isolation, Affinity Purification, Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, Incubation, Nested PCR