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anti rabbit igg hrp linked antibody for nop58  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti rabbit igg hrp linked antibody for nop58
    Anti Rabbit Igg Hrp Linked Antibody For Nop58, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg hrp linked antibody for nop58/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    anti rabbit igg hrp linked antibody for nop58 - by Bioz Stars, 2025-06
    94/100 stars

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    MG132 modulates autophagy in Ut-LMS cells. (A) Flow cytometry analysis of LC3 protein levels in MG132-treated SK-LMS-1, SK-UT-1B and SK-UT-1 cells. Cells were treated with MG132 (0.5 and 1 µM) for 24 h, followed by staining with an anti-LC3 antibody. The flow cytometry analysis quantified the number of LC3-positive cells, which reflected autophagy induction. (B) Western blot analysis of LC3 expression in MG132-treated Ut-LMS cell lines. Elevated LC3 II levels in cells indicate the conversion from LC3 I to LC3 II, a hallmark of autophagy induction. Cells were treated with MG132 (0.5 and 1 µM) for 24 h, and LC3 I and LC3 II protein expression levels were measured. Band intensities are shown in the right panel, with β-actin serving as a loading control. Data are represented as means ± SD. *P<0.05 and **P<0.01. Ut-LMS, uterine leiomyosarcoma; SD, standard deviation; LC3, light chain 3.

    Journal: Molecular Medicine Reports

    Article Title: MG132 induces cell type‑specific anticancer effects in uterine leiomyosarcoma cell lines

    doi: 10.3892/mmr.2025.13524

    Figure Lengend Snippet: MG132 modulates autophagy in Ut-LMS cells. (A) Flow cytometry analysis of LC3 protein levels in MG132-treated SK-LMS-1, SK-UT-1B and SK-UT-1 cells. Cells were treated with MG132 (0.5 and 1 µM) for 24 h, followed by staining with an anti-LC3 antibody. The flow cytometry analysis quantified the number of LC3-positive cells, which reflected autophagy induction. (B) Western blot analysis of LC3 expression in MG132-treated Ut-LMS cell lines. Elevated LC3 II levels in cells indicate the conversion from LC3 I to LC3 II, a hallmark of autophagy induction. Cells were treated with MG132 (0.5 and 1 µM) for 24 h, and LC3 I and LC3 II protein expression levels were measured. Band intensities are shown in the right panel, with β-actin serving as a loading control. Data are represented as means ± SD. *P<0.05 and **P<0.01. Ut-LMS, uterine leiomyosarcoma; SD, standard deviation; LC3, light chain 3.

    Article Snippet: Autophagy induction by MG132 was assessed using the Muse Autophagy LC3-Antibody Based Kit (MCH200109; Luminex) and Muse Cell Analyzer following the manufacturer's instructions.

    Techniques: Flow Cytometry, Staining, Western Blot, Expressing, Control, Standard Deviation

    MG132 exerts its antitumor effects in Ut-LMS cells by modulating multiple cellular pathways, including apoptosis, cell cycle regulation, apoptosis and ROS-mediated effects, in a cell-line-specific manner. LDH, lactate dehydrogenase; ROS, reactive oxygen species; NAC, N-acetyl-L-cysteine; Ut-LMS, uterine leiomyosarcoma; PARP, poly-adenosine diphosphate ribose polymerase; LC3, light chain 3; NAC, N-acetyl-L-cysteine.

    Journal: Molecular Medicine Reports

    Article Title: MG132 induces cell type‑specific anticancer effects in uterine leiomyosarcoma cell lines

    doi: 10.3892/mmr.2025.13524

    Figure Lengend Snippet: MG132 exerts its antitumor effects in Ut-LMS cells by modulating multiple cellular pathways, including apoptosis, cell cycle regulation, apoptosis and ROS-mediated effects, in a cell-line-specific manner. LDH, lactate dehydrogenase; ROS, reactive oxygen species; NAC, N-acetyl-L-cysteine; Ut-LMS, uterine leiomyosarcoma; PARP, poly-adenosine diphosphate ribose polymerase; LC3, light chain 3; NAC, N-acetyl-L-cysteine.

    Article Snippet: Autophagy induction by MG132 was assessed using the Muse Autophagy LC3-Antibody Based Kit (MCH200109; Luminex) and Muse Cell Analyzer following the manufacturer's instructions.

    Techniques: