Journal: Stem Cell Reports
Article Title: Monitoring Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes with Genetically Encoded Calcium and Voltage Fluorescent Reporters
Figure Lengend Snippet: ArcLight Expression and Imaging (A) Lentiviral transduction of the hiPSC-CMs led to robust ArcLight expression, manifested by cyclic changes in the cell fluorescence with reduced intensity during depolarization. Scale bars, 20 μm. (B and C) Optical APs derived from line-scan imaging of ArcLight-expressing hiPSC-CMs. (D) Optical APs revealing ventricular-, atrial-, and nodal-like AP morphologies. (E–G) Changes in AP morphologies (top-left images, red signals depict post-treatment tracings), following the application of blockers of the fast (E4031, 500 nM, n = 27 in three independent experiments, E) and slow (Chromanol293B, 30 μM, n = 29 in three independent experiments, F) components of the delayed rectifier potassium currents and activator of the late-sodium current (ATX-II-30nM, n = 28 in three independent experiments, G). All agents caused significant APD 90 prolongation (right) and development of EADs and triggered beats (lower). ∗ p
Article Snippet: E4031 (500 nM), ATX-II (30 nM; both from Alomone labs), ouabain (0.5–1 μM), sotalol (20 μM), isoproterenol (1 μM), and erythromycin (30 μM) were dissolved in H2O, while chromanol 293B (30 μM), cisapride (100 nM; all from Sigma), and nilotinib (1 μM; Adooq Bioscience) were dissolved in DMSO.
Techniques: Expressing, Imaging, Transduction, Fluorescence, Derivative Assay