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Corning Life Sciences attachment 6 well plates
Attachment 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/attachment 6 well plates/product/Corning Life Sciences
Average 93 stars, based on 1 article reviews
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attachment 6 well plates - by Bioz Stars, 2020-09
93/100 stars

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Irradiation:

Article Title: Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells
Article Snippet: .. Two hESC lines, RUES2 (National Institutes of Health approval no. NIHhESC-09-0013, registration no. 0013; passage 7–10) and ES02 (HES2; National Institutes of Health registry, WiCell Research Institute, Inc.; passage 3–7), were cultured on irradiated mouse embryonic fibroblasts (Global Stem; cat. no. GSC-6001G) at a density of 20,000–25,000 cells per cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), and 20 ng/ml FGF-2 (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37°C in a 5% CO2 /air environment until stem cells reached ∼90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) media of DMEM/F12 (3:1; Life Technologies) supplemented with N2 (Life Technologies), B27 (Life Technologies), ascorbic acid (50 µg/ml; Sigma), Glutamax (2 mM; Life Technologies), monothioglycerol (0.4 µM; Sigma), and 0.05% BSA (Life Technologies) at 37°C in a 5% CO2 /5% O2 /95% N2 environment. hESCs were treated with Accutase (Stemcell Technologies) and plated onto low attachment 6-well plates (Corning Inc.), resuspended in endoderm induction media containing Y-27632 (10 µM; R & D Systems), human BMP4 (0.5 ng/ml; R & D Systems), human basic fibroblast growth factor (bFGF; 2.5 ng/ml; R & D Systems); and human activin A (100 ng/ml; R & D Systems) for 72–84 h dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the embryoid bodies were dissociated into single cells using 0.05% trypsin/0.02% EDTA and plated onto fibronectin (Sigma)-coated, 24-well tissue culture plates (∼100,000–150,000 cells per well).

Article Title: Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids
Article Snippet: .. The hESC line RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37 °C in a 5% CO2/air environment until stem cells reached about 90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) medium of DMEM/F12 (3:1) (Life Technologies) supplemented with N2 (Life Technologies), B27, 50 μg/ml ascorbic acid, 2 mM Glutamax, 0.4 μM monothioglycerol, 0.05% BSA at 37 °C in a 5% CO2/5% O2/95% N2 environment. hESCs were treated with Accutase and plated onto low attachment 6-well plates (Corning Incorporated, Tewksbury MA), resuspended in endoderm induction medium containing 10 μM Y-27632, 0.5 ng/ml human BMP-4, 2.5 ng/ml human bFGF, 100 ng/ml human Activin A, for 72-84 hours dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the endoderm bodies were dissociated into single cells using 0.05% Trypsin/0.02% EDTA and plated onto fibronectin-coated, 24-well tissue culture plates (~100,000–150,000 cells/well).

Cell Culture:

Article Title: Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells
Article Snippet: .. Two hESC lines, RUES2 (National Institutes of Health approval no. NIHhESC-09-0013, registration no. 0013; passage 7–10) and ES02 (HES2; National Institutes of Health registry, WiCell Research Institute, Inc.; passage 3–7), were cultured on irradiated mouse embryonic fibroblasts (Global Stem; cat. no. GSC-6001G) at a density of 20,000–25,000 cells per cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), and 20 ng/ml FGF-2 (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37°C in a 5% CO2 /air environment until stem cells reached ∼90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) media of DMEM/F12 (3:1; Life Technologies) supplemented with N2 (Life Technologies), B27 (Life Technologies), ascorbic acid (50 µg/ml; Sigma), Glutamax (2 mM; Life Technologies), monothioglycerol (0.4 µM; Sigma), and 0.05% BSA (Life Technologies) at 37°C in a 5% CO2 /5% O2 /95% N2 environment. hESCs were treated with Accutase (Stemcell Technologies) and plated onto low attachment 6-well plates (Corning Inc.), resuspended in endoderm induction media containing Y-27632 (10 µM; R & D Systems), human BMP4 (0.5 ng/ml; R & D Systems), human basic fibroblast growth factor (bFGF; 2.5 ng/ml; R & D Systems); and human activin A (100 ng/ml; R & D Systems) for 72–84 h dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the embryoid bodies were dissociated into single cells using 0.05% trypsin/0.02% EDTA and plated onto fibronectin (Sigma)-coated, 24-well tissue culture plates (∼100,000–150,000 cells per well).

Article Title: Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids
Article Snippet: .. The hESC line RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37 °C in a 5% CO2/air environment until stem cells reached about 90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) medium of DMEM/F12 (3:1) (Life Technologies) supplemented with N2 (Life Technologies), B27, 50 μg/ml ascorbic acid, 2 mM Glutamax, 0.4 μM monothioglycerol, 0.05% BSA at 37 °C in a 5% CO2/5% O2/95% N2 environment. hESCs were treated with Accutase and plated onto low attachment 6-well plates (Corning Incorporated, Tewksbury MA), resuspended in endoderm induction medium containing 10 μM Y-27632, 0.5 ng/ml human BMP-4, 2.5 ng/ml human bFGF, 100 ng/ml human Activin A, for 72-84 hours dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the endoderm bodies were dissociated into single cells using 0.05% Trypsin/0.02% EDTA and plated onto fibronectin-coated, 24-well tissue culture plates (~100,000–150,000 cells/well).

Knock-Out:

Article Title: Generation of pulmonary neuroendocrine cells and SCLC-like tumors from human embryonic stem cells
Article Snippet: .. Two hESC lines, RUES2 (National Institutes of Health approval no. NIHhESC-09-0013, registration no. 0013; passage 7–10) and ES02 (HES2; National Institutes of Health registry, WiCell Research Institute, Inc.; passage 3–7), were cultured on irradiated mouse embryonic fibroblasts (Global Stem; cat. no. GSC-6001G) at a density of 20,000–25,000 cells per cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma-Aldrich), and 20 ng/ml FGF-2 (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37°C in a 5% CO2 /air environment until stem cells reached ∼90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) media of DMEM/F12 (3:1; Life Technologies) supplemented with N2 (Life Technologies), B27 (Life Technologies), ascorbic acid (50 µg/ml; Sigma), Glutamax (2 mM; Life Technologies), monothioglycerol (0.4 µM; Sigma), and 0.05% BSA (Life Technologies) at 37°C in a 5% CO2 /5% O2 /95% N2 environment. hESCs were treated with Accutase (Stemcell Technologies) and plated onto low attachment 6-well plates (Corning Inc.), resuspended in endoderm induction media containing Y-27632 (10 µM; R & D Systems), human BMP4 (0.5 ng/ml; R & D Systems), human basic fibroblast growth factor (bFGF; 2.5 ng/ml; R & D Systems); and human activin A (100 ng/ml; R & D Systems) for 72–84 h dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the embryoid bodies were dissociated into single cells using 0.05% trypsin/0.02% EDTA and plated onto fibronectin (Sigma)-coated, 24-well tissue culture plates (∼100,000–150,000 cells per well).

Article Title: Identification of Candidate COVID-19 Therapeutics using hPSC-derived Lung Organoids
Article Snippet: .. The hESC line RUES2 was cultured on irradiated mouse embryonic fibroblasts (Global Stem, cat. no. GSC-6001G) at a density of 20,000-25,000 cells/cm2 in a medium of DMEM/F12, 20% knockout serum replacement (Life Technologies), 0.1 mM β-mercaptoethanol (Sigma Aldrich) and 20 ng/ml bFGF (R & D Systems), and medium was changed daily. hESC cultures were maintained in an undifferentiated state at 37 °C in a 5% CO2/air environment until stem cells reached about 90% confluence. hESC differentiation into endoderm was performed in serum-free differentiation (SFD) medium of DMEM/F12 (3:1) (Life Technologies) supplemented with N2 (Life Technologies), B27, 50 μg/ml ascorbic acid, 2 mM Glutamax, 0.4 μM monothioglycerol, 0.05% BSA at 37 °C in a 5% CO2/5% O2/95% N2 environment. hESCs were treated with Accutase and plated onto low attachment 6-well plates (Corning Incorporated, Tewksbury MA), resuspended in endoderm induction medium containing 10 μM Y-27632, 0.5 ng/ml human BMP-4, 2.5 ng/ml human bFGF, 100 ng/ml human Activin A, for 72-84 hours dependent on the formation rates of endoderm cells. .. On day 3 or 3.5, the endoderm bodies were dissociated into single cells using 0.05% Trypsin/0.02% EDTA and plated onto fibronectin-coated, 24-well tissue culture plates (~100,000–150,000 cells/well).

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    Corning Life Sciences ultra low attachment 6 well plates
    Clonogenic and self-renewal potential of MDA-MB-231 cells cultured on a 2D surface or within Col-I gels. (A–D) Evaluation of the clonogenic potential of MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected and processed to obtain individualized cell suspension. After plating (500 cells/35 mm plate), cells were cultured for 14 days and then washed, fixed and stained with crystal violet. The images were acquired with a transilluminator and represent technical replicates of one independent experiment. (E) Bar graphs represent mean ± SD of five independent experiments performed in technical quadruplicates. (F–I) Evaluation of mammosphere (MS)-formation efficiency by MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected, processed to obtain individualized cell suspension and grown in ultra-low attachment 6-well plates (5,000 cells/well) with culture medium enriched for MS formation. After 10 days, images of at least 25 fields per well were acquired. Dashed squares outline zoomed-in MS, which have a minimum diameter of 50 μm (scale) and compact shape. Bar graphs (J) and scatter plots (K–M) represent mean ± SD of five independent experiments, performed in technical triplicate.
    Ultra Low Attachment 6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra low attachment 6 well plates/product/Corning Life Sciences
    Average 89 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    ultra low attachment 6 well plates - by Bioz Stars, 2020-09
    89/100 stars
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    Clonogenic and self-renewal potential of MDA-MB-231 cells cultured on a 2D surface or within Col-I gels. (A–D) Evaluation of the clonogenic potential of MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected and processed to obtain individualized cell suspension. After plating (500 cells/35 mm plate), cells were cultured for 14 days and then washed, fixed and stained with crystal violet. The images were acquired with a transilluminator and represent technical replicates of one independent experiment. (E) Bar graphs represent mean ± SD of five independent experiments performed in technical quadruplicates. (F–I) Evaluation of mammosphere (MS)-formation efficiency by MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected, processed to obtain individualized cell suspension and grown in ultra-low attachment 6-well plates (5,000 cells/well) with culture medium enriched for MS formation. After 10 days, images of at least 25 fields per well were acquired. Dashed squares outline zoomed-in MS, which have a minimum diameter of 50 μm (scale) and compact shape. Bar graphs (J) and scatter plots (K–M) represent mean ± SD of five independent experiments, performed in technical triplicate.

    Journal: PeerJ

    Article Title: High type I collagen density fails to increase breast cancer stem cell phenotype

    doi: 10.7717/peerj.9153

    Figure Lengend Snippet: Clonogenic and self-renewal potential of MDA-MB-231 cells cultured on a 2D surface or within Col-I gels. (A–D) Evaluation of the clonogenic potential of MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected and processed to obtain individualized cell suspension. After plating (500 cells/35 mm plate), cells were cultured for 14 days and then washed, fixed and stained with crystal violet. The images were acquired with a transilluminator and represent technical replicates of one independent experiment. (E) Bar graphs represent mean ± SD of five independent experiments performed in technical quadruplicates. (F–I) Evaluation of mammosphere (MS)-formation efficiency by MDA-MB-231 cells previously cultured on a 2D surface or within Col-I gels. Cells were collected, processed to obtain individualized cell suspension and grown in ultra-low attachment 6-well plates (5,000 cells/well) with culture medium enriched for MS formation. After 10 days, images of at least 25 fields per well were acquired. Dashed squares outline zoomed-in MS, which have a minimum diameter of 50 μm (scale) and compact shape. Bar graphs (J) and scatter plots (K–M) represent mean ± SD of five independent experiments, performed in technical triplicate.

    Article Snippet: Analysis of self-renewal potential Cells previously cultured on 2D surface (5 × 103 cells/plate) or within Col-I gels (25 × 103 cells/gel) for 7 days were collected, dissociated, and cell suspension was plated in ultra-low attachment 6-well plates (Corning Inc., Corning, NY, USA) at low cell density (5 × 103 cells/well) in DMEM-F12 containing 20 ng/ml bFGF (Sigma, Ronkonkoma, NY, USA), 20 ng/ml EGF (Sigma, Ronkonkoma, NY, USA) and 1X B-27 supplement (Gibco, Langley, OK, USA).

    Techniques: Multiple Displacement Amplification, Cell Culture, Staining

    Self-renewal of mammospheres derived from MCF7. (A) The Sphere Formation Efficiency (SFE) was determined in MCF7-Tet-On-SrcDN. Thus, single cell suspensions from adherent cells were plated in 6-well ultralow attachment plates at 2x10 3 cells/well and cultured in mammosphere medium. Fifteen days later, mammospheres were dissociated into single cells that were divided into two groups: Control (-Doxy) and Doxy treated (2 μg/mL). Doxy-treatment was maintained for three generations, and renewed every 3 days. SFE at 3 rd generation was calculated as number of spheres formed per number of seeded cells and expressed as % means ± SD. The SFE experiments were repeated 5 times, each one of them was carried out in triplicates. (p

    Journal: PLoS ONE

    Article Title: c-Src functionality controls self-renewal and glucose metabolism in MCF7 breast cancer stem cells

    doi: 10.1371/journal.pone.0235850

    Figure Lengend Snippet: Self-renewal of mammospheres derived from MCF7. (A) The Sphere Formation Efficiency (SFE) was determined in MCF7-Tet-On-SrcDN. Thus, single cell suspensions from adherent cells were plated in 6-well ultralow attachment plates at 2x10 3 cells/well and cultured in mammosphere medium. Fifteen days later, mammospheres were dissociated into single cells that were divided into two groups: Control (-Doxy) and Doxy treated (2 μg/mL). Doxy-treatment was maintained for three generations, and renewed every 3 days. SFE at 3 rd generation was calculated as number of spheres formed per number of seeded cells and expressed as % means ± SD. The SFE experiments were repeated 5 times, each one of them was carried out in triplicates. (p

    Article Snippet: Briefly, single cell suspensions of adherent cultures were plated in 6-well ultralow attachment plates (Falcon, Corning Life Science) at 2x103 cells/well and maintained in serum-free DMEM/F12 media (1:1), BSA (4 mg/mL), EGF (20 ng/mL) and bFGF (20 ng/mL), insulin (5 μg/mL), hydrocortisone (5 μg/mL) to obtain mammospheres.

    Techniques: Derivative Assay, Cell Culture

    Treatment with TF3 at designated concentration inhibited tumorsphere formation of A2780/CP70 and OVCAR3 CSCs. The cells were treated with TF3 for 24h and cultured in drug-free medium in 6-well ultra-low attachment plates at 2×10 3 cells/well for 6 days to form spheroids. The tumorspheres were detected and photographed by Zeiss microscopy (magnification, ×40). Scale bar size is 100 μm.

    Journal: Journal of functional foods

    Article Title: Theaflavin-3, 3’-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway

    doi: 10.1016/j.jff.2018.09.021

    Figure Lengend Snippet: Treatment with TF3 at designated concentration inhibited tumorsphere formation of A2780/CP70 and OVCAR3 CSCs. The cells were treated with TF3 for 24h and cultured in drug-free medium in 6-well ultra-low attachment plates at 2×10 3 cells/well for 6 days to form spheroids. The tumorspheres were detected and photographed by Zeiss microscopy (magnification, ×40). Scale bar size is 100 μm.

    Article Snippet: The suspended cells were seeded with 2 mL in 6-well ultra-low attachment plates (Corning) and cultured for 6 days to form 1st generation tumor spheres.

    Techniques: Concentration Assay, Cell Culture, Microscopy