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Tocris atpase inhibitor
Atpase Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atpase inhibitor/product/Tocris
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
atpase inhibitor - by Bioz Stars, 2020-05
92/100 stars

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Article Title: Elevated hydrostatic pressure stimulates ATP release which mediates activation of the NLRP3 inflammasome via P2X4 in rat urothelial cells
Article Snippet: 60 minutes prior to pressure exposure, the culture media was replaced with serum free F-12K media containing 100 µM ATPase inhibitor (Tocris Bioscience, Bristol, UK) in order to prevent degradation of extracellular ATP before measurements could be taken.

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  • 94
    Tocris chemical reagents blebbistatin
    Contractility couples morphogenesis and fate specification (a-b) Immunostaining of doublets of 16-cell stage blastomeres treated with 25 µM <t>Blebbistatin</t> (Bb) (+) (a, an inactive enantiomere of the inhibitor) or Bb(-) (b, the selective inhibitor of myosin II ATPase activity) showing pYap (green), Cdx2 (red) and phalloidin (blue). (c-f) Nucleus to cytoplasm intensity ratio of pYap (c) or Cdx2 (e) as a function of the internal contact angle for doublets treated with Bb(+) (outer cells in orange and inner cells in blue, pYap R = -0.630, or Cdx2 R = -0.493, n = 59 doublets from 3 experiments, p
    Chemical Reagents Blebbistatin, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemical reagents blebbistatin/product/Tocris
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chemical reagents blebbistatin - by Bioz Stars, 2020-05
    94/100 stars
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    95
    Tocris cd39 inhibitor
    GMSCs inhibit T cell proliferation through <t>CD39/CD73</t> and IDO signals . (A,B) Human CD3 + T cells were prepared from PBMC and labeled with CFSE. T cells were then stimulated with irradiated non T cells (1:4) and anti-CD3 (1 µg/ml) for 4 days. Human fibroblast cells were used as a negative control. The T cell proliferation was determined by Flow cytometry. A representative experiment is shown in panel (B) . (C) GMSCs were cocultured with CD3 + T cells as described above. Graded doses of GMSCs were added to T cells for 4 days. (D) GMSC were cocultured with CD3 + T cells, ratio of GMSC to T cells was 1:5. Different reagents as described above were added to the coculture system, and the suppression rate was determined by Flow cytometry. The same ratio of GMSC and reagents were added as in panels (C,D) . CFSE dilution was determined by FACS. Data are summarized from three replicate experiments, represented as mean ± SEM. ** p
    Cd39 Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd39 inhibitor/product/Tocris
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cd39 inhibitor - by Bioz Stars, 2020-05
    95/100 stars
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    95
    Tocris blebbistatin
    ROCK activation promotes collagen degradation Representative fluorescence images of cells treated with 1 μM 4HT in the absence or presence of 10 μM H1152 for 18 h on Collagen1‐FITC (Col1‐FITC). Co‐staining for F‐actin, MMP10 (red), and DAPI (blue). Scale bar = 10 μm. Quantification of collagen degradation by cells treated with 1 μM 4HT or 4HT + 10 μM H1152 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Quantification of collagen degradation by cells treated with 1 μM 4HT, 4HT + DMSO vehicle, 4HT + 50 μM <t>Blebbistatin</t> or 4HT + 10 μM GM6001 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Immunofluorescence of collagen matrix sections co‐stained for MMP10 and DAPI. Scale bar = 20 μm. Mmp13 in situ hybridization‐stained sections of collagen matrices. Scale bar = 50 μm.
    Blebbistatin, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blebbistatin/product/Tocris
    Average 95 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    blebbistatin - by Bioz Stars, 2020-05
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    Contractility couples morphogenesis and fate specification (a-b) Immunostaining of doublets of 16-cell stage blastomeres treated with 25 µM Blebbistatin (Bb) (+) (a, an inactive enantiomere of the inhibitor) or Bb(-) (b, the selective inhibitor of myosin II ATPase activity) showing pYap (green), Cdx2 (red) and phalloidin (blue). (c-f) Nucleus to cytoplasm intensity ratio of pYap (c) or Cdx2 (e) as a function of the internal contact angle for doublets treated with Bb(+) (outer cells in orange and inner cells in blue, pYap R = -0.630, or Cdx2 R = -0.493, n = 59 doublets from 3 experiments, p

    Journal: Nature

    Article Title: Asymmetric division of contractile domains couples cell positioning and fate specification

    doi: 10.1038/nature18958

    Figure Lengend Snippet: Contractility couples morphogenesis and fate specification (a-b) Immunostaining of doublets of 16-cell stage blastomeres treated with 25 µM Blebbistatin (Bb) (+) (a, an inactive enantiomere of the inhibitor) or Bb(-) (b, the selective inhibitor of myosin II ATPase activity) showing pYap (green), Cdx2 (red) and phalloidin (blue). (c-f) Nucleus to cytoplasm intensity ratio of pYap (c) or Cdx2 (e) as a function of the internal contact angle for doublets treated with Bb(+) (outer cells in orange and inner cells in blue, pYap R = -0.630, or Cdx2 R = -0.493, n = 59 doublets from 3 experiments, p

    Article Snippet: Chemical reagents Blebbistatin (+), an inactive enantiomere of the inhibitor, or (-), the selective inhibitor of myosin II ATPase activity, (Tocris, 1853 and 1852) 50 mM DMSO stocks are diluted to 5, 12.5 or 25 µM in KSOM.

    Techniques: Immunostaining, Activity Assay

    GMSCs inhibit T cell proliferation through CD39/CD73 and IDO signals . (A,B) Human CD3 + T cells were prepared from PBMC and labeled with CFSE. T cells were then stimulated with irradiated non T cells (1:4) and anti-CD3 (1 µg/ml) for 4 days. Human fibroblast cells were used as a negative control. The T cell proliferation was determined by Flow cytometry. A representative experiment is shown in panel (B) . (C) GMSCs were cocultured with CD3 + T cells as described above. Graded doses of GMSCs were added to T cells for 4 days. (D) GMSC were cocultured with CD3 + T cells, ratio of GMSC to T cells was 1:5. Different reagents as described above were added to the coculture system, and the suppression rate was determined by Flow cytometry. The same ratio of GMSC and reagents were added as in panels (C,D) . CFSE dilution was determined by FACS. Data are summarized from three replicate experiments, represented as mean ± SEM. ** p

    Journal: Frontiers in Immunology

    Article Title: Human Gingiva-Derived Mesenchymal Stem Cells Inhibit Xeno-Graft-versus-Host Disease via CD39–CD73–Adenosine and IDO Signals

    doi: 10.3389/fimmu.2017.00068

    Figure Lengend Snippet: GMSCs inhibit T cell proliferation through CD39/CD73 and IDO signals . (A,B) Human CD3 + T cells were prepared from PBMC and labeled with CFSE. T cells were then stimulated with irradiated non T cells (1:4) and anti-CD3 (1 µg/ml) for 4 days. Human fibroblast cells were used as a negative control. The T cell proliferation was determined by Flow cytometry. A representative experiment is shown in panel (B) . (C) GMSCs were cocultured with CD3 + T cells as described above. Graded doses of GMSCs were added to T cells for 4 days. (D) GMSC were cocultured with CD3 + T cells, ratio of GMSC to T cells was 1:5. Different reagents as described above were added to the coculture system, and the suppression rate was determined by Flow cytometry. The same ratio of GMSC and reagents were added as in panels (C,D) . CFSE dilution was determined by FACS. Data are summarized from three replicate experiments, represented as mean ± SEM. ** p

    Article Snippet: Different reagents, including selective heme oxygenase1 (HO-1) inhibitor [50 ng/ml zinc protoporphyrin IX (ZnPP); Frontier Scientific], HO-1 inducer (50 ng/ml hemin; Sigma-Aldrich), nitric oxide (NO) synthase inhibitor [1 mM l -NG nitroarginine methyl ester hydrochloride (l -NAME); Sigma-Aldrich], indoleamine 2,3-dioxygenase (IDO) inhibitor [500 μM l -1-methyltryptophan (1-MT); Sigma-Aldrich], CD73 inhibitor [100–500 µM α,β-methylene ADP (APCP); Sigma-Aldrich], a CD39 inhibitor [100 µM sodium polyoxot-ungstate 1 (POM1); Tocris Bioscience], selective cyclooxygenase 1 (COX-1) inhibitor (20 µM indomethacine; Sigma-Aldrich), selective COX-2 inhibitor (10 µM NS398; Tocris Bioscience), ALK5 inhibitor (10 µM), anti-transforming growth factor β (anti-TGF-β) antibody (10 µg/ml; BD PharMingen), selective A2B adenosine receptor antagonist (10 µM alloxazine; Sigma-Aldrich), selective A2A adenosine receptor competitive antagonist (25 µM SCH58261; Tocris Bioscience), were added to the coculture system, and the suppression rate was determined by Flow Cytometry.

    Techniques: Labeling, Irradiation, Negative Control, Flow Cytometry, Cytometry, FACS

    GMSCs inhibit xenogenic GVHD through CD39 and/or IDO signaling pathways . NOD/SCID mice were injected with 20 × 10 6 PBMC with or without 2 × 10 6 GMSCs ( n = 8). (A,B) Some GMSC cells were pretreated with POM-1 (100 µm) overnight before co-injection with PBMC ( n = 11). (C,D) In another group, mice giving human cell alone or human cells plus GMSCs were injected i.p. with 1-MT at 10 mg/mouse/day for 14 consecutive days ( n = 10) and then monitored for survival and weight loss. Kaplan–Meier survival curves depict the percentage of live mice. (B,D) The weight loss data were presented as the Mean ± SEM from two independent experiments. ** p

    Journal: Frontiers in Immunology

    Article Title: Human Gingiva-Derived Mesenchymal Stem Cells Inhibit Xeno-Graft-versus-Host Disease via CD39–CD73–Adenosine and IDO Signals

    doi: 10.3389/fimmu.2017.00068

    Figure Lengend Snippet: GMSCs inhibit xenogenic GVHD through CD39 and/or IDO signaling pathways . NOD/SCID mice were injected with 20 × 10 6 PBMC with or without 2 × 10 6 GMSCs ( n = 8). (A,B) Some GMSC cells were pretreated with POM-1 (100 µm) overnight before co-injection with PBMC ( n = 11). (C,D) In another group, mice giving human cell alone or human cells plus GMSCs were injected i.p. with 1-MT at 10 mg/mouse/day for 14 consecutive days ( n = 10) and then monitored for survival and weight loss. Kaplan–Meier survival curves depict the percentage of live mice. (B,D) The weight loss data were presented as the Mean ± SEM from two independent experiments. ** p

    Article Snippet: Different reagents, including selective heme oxygenase1 (HO-1) inhibitor [50 ng/ml zinc protoporphyrin IX (ZnPP); Frontier Scientific], HO-1 inducer (50 ng/ml hemin; Sigma-Aldrich), nitric oxide (NO) synthase inhibitor [1 mM l -NG nitroarginine methyl ester hydrochloride (l -NAME); Sigma-Aldrich], indoleamine 2,3-dioxygenase (IDO) inhibitor [500 μM l -1-methyltryptophan (1-MT); Sigma-Aldrich], CD73 inhibitor [100–500 µM α,β-methylene ADP (APCP); Sigma-Aldrich], a CD39 inhibitor [100 µM sodium polyoxot-ungstate 1 (POM1); Tocris Bioscience], selective cyclooxygenase 1 (COX-1) inhibitor (20 µM indomethacine; Sigma-Aldrich), selective COX-2 inhibitor (10 µM NS398; Tocris Bioscience), ALK5 inhibitor (10 µM), anti-transforming growth factor β (anti-TGF-β) antibody (10 µg/ml; BD PharMingen), selective A2B adenosine receptor antagonist (10 µM alloxazine; Sigma-Aldrich), selective A2A adenosine receptor competitive antagonist (25 µM SCH58261; Tocris Bioscience), were added to the coculture system, and the suppression rate was determined by Flow Cytometry.

    Techniques: Mouse Assay, Injection

    ROCK activation promotes collagen degradation Representative fluorescence images of cells treated with 1 μM 4HT in the absence or presence of 10 μM H1152 for 18 h on Collagen1‐FITC (Col1‐FITC). Co‐staining for F‐actin, MMP10 (red), and DAPI (blue). Scale bar = 10 μm. Quantification of collagen degradation by cells treated with 1 μM 4HT or 4HT + 10 μM H1152 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Quantification of collagen degradation by cells treated with 1 μM 4HT, 4HT + DMSO vehicle, 4HT + 50 μM Blebbistatin or 4HT + 10 μM GM6001 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Immunofluorescence of collagen matrix sections co‐stained for MMP10 and DAPI. Scale bar = 20 μm. Mmp13 in situ hybridization‐stained sections of collagen matrices. Scale bar = 50 μm.

    Journal: EMBO Molecular Medicine

    Article Title: ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth

    doi: 10.15252/emmm.201606743

    Figure Lengend Snippet: ROCK activation promotes collagen degradation Representative fluorescence images of cells treated with 1 μM 4HT in the absence or presence of 10 μM H1152 for 18 h on Collagen1‐FITC (Col1‐FITC). Co‐staining for F‐actin, MMP10 (red), and DAPI (blue). Scale bar = 10 μm. Quantification of collagen degradation by cells treated with 1 μM 4HT or 4HT + 10 μM H1152 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Quantification of collagen degradation by cells treated with 1 μM 4HT, 4HT + DMSO vehicle, 4HT + 50 μM Blebbistatin or 4HT + 10 μM GM6001 for 16 h. Means ± SEM ( n = 15), one‐way ANOVA with multiplicity adjusted exact P ‐value by post hoc Tukey multiple comparison test. Immunofluorescence of collagen matrix sections co‐stained for MMP10 and DAPI. Scale bar = 20 μm. Mmp13 in situ hybridization‐stained sections of collagen matrices. Scale bar = 50 μm.

    Article Snippet: Small molecules Y27632 (Tocris 1254), H1152 (Tocris 2414), Fasudil (Selleckchem S1573, LC Laboratories F‐4660), Blebbistatin (Tocris 1760), GM6001 (Millipore 142880‐36‐2), Tamoxifen citrate salt (Sigma T9262), 4HT (4‐Hydroxytamoxifen, Sigma H7904).

    Techniques: Activation Assay, Fluorescence, Staining, Immunofluorescence, In Situ Hybridization

    ( A – C ) Maximal projection of image z-stack of MDCK cells treated with blebbistatin on ( A ) a flat substrate, a cylinder substrate of radius of curvature R c , ( B ) 40 μ m, and ( C ) 20 μ m, with corresponding xz-cross-sectional view below each image. Blue corresponds to nucleus, green corresponds to E-cadherin, and red corresponds to F-actin. Scale bar represents 20 μ m. ( D ) Angular distribution of MDCK cells treated with blebbistatin on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( E – G ) Maximal projection of image z-stack of MDCK cells treated with calyculin A on ( E ) a flat substrate, a cylinder substrate of radius of curvature R c , ( F ) 40 μ m, and ( G ) 20 μ m, with corresponding xz-cross-sectional view below each image, is shown. Scale bar represents 20 μ m. ( H ) Angular distribution of MDCK cells treated with calyculin A, on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( I – K ) Maximal projection of image z-stack of MDCK cells treated with EGTA on ( I ) a flat substrate, a cylinder substrate of radius of curvature R c , ( J ) 40 μ m, and ( K ) 20 μ m, with corresponding xz-cross-sectional view below each image, is shown. Scale bar represents 20 μ m. ( L ) Angular distribution of MDCK cells treated with EGTA, on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( M ) Percentage of MDCK cells with various treatment that lie within 20° of the axis of the channel versus radius of curvature R c is shown. Error bars indicate standard error of the mean. ( N ) Elongation of MDCK cells with various treatment versus radius of curvature R c is shown . ∗ and ∗∗∗ represent p

    Journal: Biophysical Journal

    Article Title: Cell-Cell Adhesion and Cortical Actin Bending Govern Cell Elongation on Negatively Curved Substrates

    doi: 10.1016/j.bpj.2018.02.027

    Figure Lengend Snippet: ( A – C ) Maximal projection of image z-stack of MDCK cells treated with blebbistatin on ( A ) a flat substrate, a cylinder substrate of radius of curvature R c , ( B ) 40 μ m, and ( C ) 20 μ m, with corresponding xz-cross-sectional view below each image. Blue corresponds to nucleus, green corresponds to E-cadherin, and red corresponds to F-actin. Scale bar represents 20 μ m. ( D ) Angular distribution of MDCK cells treated with blebbistatin on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( E – G ) Maximal projection of image z-stack of MDCK cells treated with calyculin A on ( E ) a flat substrate, a cylinder substrate of radius of curvature R c , ( F ) 40 μ m, and ( G ) 20 μ m, with corresponding xz-cross-sectional view below each image, is shown. Scale bar represents 20 μ m. ( H ) Angular distribution of MDCK cells treated with calyculin A, on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( I – K ) Maximal projection of image z-stack of MDCK cells treated with EGTA on ( I ) a flat substrate, a cylinder substrate of radius of curvature R c , ( J ) 40 μ m, and ( K ) 20 μ m, with corresponding xz-cross-sectional view below each image, is shown. Scale bar represents 20 μ m. ( L ) Angular distribution of MDCK cells treated with EGTA, on substrates of various radius of curvature R c , with 0° and 90° being perpendicular and parallel to the long axis of the cylinder, is shown. ( M ) Percentage of MDCK cells with various treatment that lie within 20° of the axis of the channel versus radius of curvature R c is shown. Error bars indicate standard error of the mean. ( N ) Elongation of MDCK cells with various treatment versus radius of curvature R c is shown . ∗ and ∗∗∗ represent p

    Article Snippet: For cells treated with EGTA, blebbistatin, or calyculin A, final concentrations of 4 mM EGTA (Sigma), 100 μL/mL blebbistatin (Tocris Bioscience, Bristol, United Kingdom), or 10 nM calyculin A (Sigma) were added to the respective samples 4 h before fixing.

    Techniques: