atpase antibody  (Millipore)


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    Structured Review

    Millipore atpase antibody
    Atpase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase antibody/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atpase antibody - by Bioz Stars, 2020-05
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells
    Article Snippet: .. To study cellular targeting of uPA, uPAR, and PAI-1-luciferase (luc) expression, transfected primary human gastric glands were double immunostained with goat anti-luciferase antibody (Rockland Immunochemicals, Gilbertsville, PA) together with one of the following: rabbit anti-pepsinogen (gift f;rom Mike Samloff, Center for Ulcer Research, Los Angeles, CA), rabbit anti-H+ /K+ ATPase (Calbiochem), rabbit anti-vesicular monoamine transporter 2 , mouse anti-trefoil factor-1 (TFF-1; Dako, Glostrup, Denmark), and mouse anti-TFF-2 (NovoCastra, Newcastle-upon-Tyne, UK) antibodies with appropriate FITC or Texas red-conjugated secondary antibodies (Jackson Immunoresearch, Soham, UK), using Vectashield mounting medium with DAPI (Vector Laboratories, Peterborough, UK) to counterstain nuclei. .. Slides were examined using a Zeiss Axioplan-2 microscope (Zeiss Vision, Welwyn Garden City, UK) and images captured using a JVC-3 charge-coupled device camera and KS300 software combined with a deconvolution software (Imaging Associates, Bicester, UK).

    Protease Inhibitor:

    Article Title: Cardiac Steroids Induce Changes in Recycling of the Plasma Membrane in Human NT2 Cells
    Article Snippet: .. Bufalin, etoposide, monoclonal antibodies anti-human Na+ , K+ -ATPase α subunits, clone M7-PB-E9, protease inhibitor cocktail [aprotinin 80 μM, leupeptin 2.2 mM, bestatin 4 mM, pepstatin 1.5 mM, E-64 1.4 mM, 4-(2-aminoethyl)benzenesulfonyl fluoride 100 mM], and geneticin disulfate salt (G418) were purchased from Sigma-Aldrich (St. Louis, MO.). .. FuGENE 6 transfection reagent was obtained from Roche Diagnostics (Indianapolis, IN), pCI-neo vector from Promega (Madison, WI), and pCMV Ouabainr vector from BD Biosciences PharMingen (San Diego, CA).

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Labeling:

    Article Title: Inefficient Chronic Activation of Parietal Cells in Ae2a,b−/− Mice
    Article Snippet: .. Alternatively, sections incubated with the H+ /K+ -ATPase antibody were further treated with phalloidin-tetramethyl-rhodamine isothiocyanate (TRITC) labeled from Sigma (reference no. P1951) in PBS supplemented with 0.1% Triton X-100 and 3% bovine serum albumin (20 minutes), followed by incubation with the Alexa-labeled anti-mouse secondary antibody. ..

    Incubation:

    Article Title: Inefficient Chronic Activation of Parietal Cells in Ae2a,b−/− Mice
    Article Snippet: .. Alternatively, sections incubated with the H+ /K+ -ATPase antibody were further treated with phalloidin-tetramethyl-rhodamine isothiocyanate (TRITC) labeled from Sigma (reference no. P1951) in PBS supplemented with 0.1% Triton X-100 and 3% bovine serum albumin (20 minutes), followed by incubation with the Alexa-labeled anti-mouse secondary antibody. ..

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    other:

    Article Title: Aqp2-Expressing Cells Give Rise to Renal Intercalated Cells
    Article Snippet: Wade, University of Maryland School, Baltimore, MD); chicken anti–V-ATPase A and anti–V-ATPase B1; rabbit anti-H3 (Millipore); anti-H3 mono-, di-, and tri-methyl K79 (abcom); anti-Cre (Sigma); anti-AE1 (Alpha Diagnostic); and four antibodies (rabbit anti–V-ATPase B1 and B2, goat anti-AQP2, mouse anti–V-ATPase B1 and B2, and mouse anti-CAII) from Santa Cruz.

    Expressing:

    Article Title: Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells
    Article Snippet: .. To study cellular targeting of uPA, uPAR, and PAI-1-luciferase (luc) expression, transfected primary human gastric glands were double immunostained with goat anti-luciferase antibody (Rockland Immunochemicals, Gilbertsville, PA) together with one of the following: rabbit anti-pepsinogen (gift f;rom Mike Samloff, Center for Ulcer Research, Los Angeles, CA), rabbit anti-H+ /K+ ATPase (Calbiochem), rabbit anti-vesicular monoamine transporter 2 , mouse anti-trefoil factor-1 (TFF-1; Dako, Glostrup, Denmark), and mouse anti-TFF-2 (NovoCastra, Newcastle-upon-Tyne, UK) antibodies with appropriate FITC or Texas red-conjugated secondary antibodies (Jackson Immunoresearch, Soham, UK), using Vectashield mounting medium with DAPI (Vector Laboratories, Peterborough, UK) to counterstain nuclei. .. Slides were examined using a Zeiss Axioplan-2 microscope (Zeiss Vision, Welwyn Garden City, UK) and images captured using a JVC-3 charge-coupled device camera and KS300 software combined with a deconvolution software (Imaging Associates, Bicester, UK).

    Affinity Purification:

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Binding Assay:

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

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  • 93
    Millipore mouse anti na k atpase α
    Assessment of subcellular contamination. Representative immunoblots showing expressions of COx ( A ), GAPDH ( B ), Na +- K + <t>-ATPase-α</t> ( C ), TGFβ-R2 ( D ), and GLUT1 ( E ) in WH, Cyto, and MI from normal primary breast cell line (HMEC 184) and breast
    Mouse Anti Na K Atpase α, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti na k atpase α/product/Millipore
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mouse anti na k atpase α - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Millipore polyclonal rabbit anti α1 na k atpase
    Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney <t>α1</t> <t>Na/K-ATPase</t> (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p
    Polyclonal Rabbit Anti α1 Na K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti α1 na k atpase/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti α1 na k atpase - by Bioz Stars, 2020-05
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    85
    Millipore rabbit anti nkaα3
    Western blot analysis of the specificity of NKAα1 and α3 antibodies. Brain and spinal cord homogenates were probed with antibodies raised against either NKAα1 or <t>NKAα3.</t> Both antibodies recognized a single band in all homogenates.
    Rabbit Anti Nkaα3, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nkaα3/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nkaα3 - by Bioz Stars, 2020-05
    85/100 stars
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    Image Search Results


    Assessment of subcellular contamination. Representative immunoblots showing expressions of COx ( A ), GAPDH ( B ), Na +- K + -ATPase-α ( C ), TGFβ-R2 ( D ), and GLUT1 ( E ) in WH, Cyto, and MI from normal primary breast cell line (HMEC 184) and breast

    Journal: Physiological Genomics

    Article Title: Mitochondrial and plasma membrane lactate transporter and lactate dehydrogenase isoform expression in breast cancer cell lines

    doi: 10.1152/physiolgenomics.00177.2010

    Figure Lengend Snippet: Assessment of subcellular contamination. Representative immunoblots showing expressions of COx ( A ), GAPDH ( B ), Na +- K + -ATPase-α ( C ), TGFβ-R2 ( D ), and GLUT1 ( E ) in WH, Cyto, and MI from normal primary breast cell line (HMEC 184) and breast

    Article Snippet: Primary antibodies used were rabbit anti-MCT1, and rabbit anti-MCT4 (Brooks, polyclonal custom antibody), rabbit anti-MCT2 (Chemicon International, Temecula, CA), rabbit and mouse anti-cytochrome oxidase subunit IV, and goat anti-LDH, that reacts with all LDH isoforms (see ; Abcam, Cambridge, MA), rabbit anti-LDHA and rabbit anti-LDHB (Sigma-Aldrich), goat anti-CD147 (Research Diagnosis, Flanders, NJ), rabbit anti-β-actin, mouse anti-GAPDH (Imgenex, San Diego, CA), mouse anti-Na+ -K+ -ATPase-α (Upstate, Millipore Corporate, MA), rabbit anti-GLUT1, and rabbit anti-TGFβ-R2 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Techniques: Western Blot

    Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Na,K-ATPase β 2 and α 2 subunits are localized almost exclusively in T-tubules in cardiomyocytes, whereas the α 1 and β 1 subunits are localized in both sarcolemma and T-tubules. A , frozen sections of rat heart were double-stained

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Staining

    Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Expression of purified Na,K-ATPase isoforms. Coomassie-stained SDS-PAGE of purified isoforms (5 μg/lane). For deglycosylation, samples were denatured and treated with PNGase F for 60 min at 37 °C.

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Expressing, Purification, Staining, SDS Page

    Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Models for docking of DcB to α 1 β 1 , α 2 β 2 , and α 2 β 3 . DcB was docked into homology models of human Na,K-ATPase isoforms derived from the porcine E2P·Mg·digoxin structure (Protein Data Bank code

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Derivative Assay

    The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit preferentially interact with each other in mouse heart. A , Western blotting analysis of proteins immunoprecipitated and co-immunoprecipitated from the detergent extracts of mouse heart microsome

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Western Blot, Immunoprecipitation

    The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: The Na,K-ATPase α 2 subunit and β 2 subunit are preferentially expressed in the mouse embryonic heart in contrast to the ubiquitously expressed α 1 subunit. A , paraffin-embedded sections of mouse embryos (embryonic day 12.5) were

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques:

    Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Potassium-digoxin antagonism depicted for α 1 β 1 , α 2 β 1 , α 2 β 2 , and α 2 β 3 . Inhibition of purified Na,K-ATPase isoforms by digoxin was measured, and K i values were plotted against the potassium

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Inhibition, Purification

    Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Potassium-[ 3 H]digoxin antagonism. Yeast membranes harboring α 2 β 1 , α 2 β 2 , or α 2 β 3 Na,K-ATPase were incubated with varying concentrations of KCl, and residual [ 3 H]digoxin binding was measured. The lines represent

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Incubation, Binding Assay

    Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Journal: The Journal of Biological Chemistry

    Article Title: Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

    doi: 10.1074/jbc.M116.751735

    Figure Lengend Snippet: Inhibition of Na,K-ATPase Isoforms by Perhydro-1,4-oxazepine Derivatives of Digoxin

    Article Snippet: The precleared cell extract was mixed with 10 μl of polyclonal antibodies against the Na,K-ATPase α2 subunit (Millipore) or 10 μl of polyclonal antibodies against the Na,K-ATPase α subunit ( ) or 10 μl of polyclonal antibodies against the Na,K-ATPase β2 subunit (Millipore) and incubated with continuous rotation at 4 °C for 60 min. After the addition of 30 μl of the protein A-agarose suspension, the mixture was incubated at 4 °C with continuous rotation overnight.

    Techniques: Inhibition

    Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney α1 Na/K-ATPase (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Y260 phosphorylation and Src- (a) Y260 phosphorylation in purified pig kidney α1 Na/K-ATPase (2 µg) by Src (4.5 units) in presence of 2 mM Mg 2+ -ATP in 10 minutes. Representative blots are shown, n = 3. Control blots showing Src phosphorylation at Y418 and Y529 sites by Mg 2+ -ATP are shown. (b) Effects of PP2 (5 μM, 30 minutes) on tyrosine phosphorylation of α1 Na/K-ATPase in LLC-PK1 cells. A representative immunoblot is shown. n = 4–5. (c) Effects of Src family kinase knockout on Y260 phosphorylation. Cell lysates were prepared from Src, Yes, Fyn knockout SYF, and Src-rescued SYF cells. Representative blots are shown, n = 4. (d) Effects of ouabain on Y260 phosphorylation as a function of time in LLC-PK1 cells. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Purification, Knock-Out

    Schematic diagrams of α1 Na/K-ATPase-mediated Src regulation in normal and cancer cells. Established signaling pathways are denoted by solid black arrows and speculated signaling pathways are denoted by broken black arrows. NKA = Na/K-ATPase, Cav = Caveolin 1, FAK = Focal Adhesion Kinase, RTK = Receptor Tyrosine Kinase, PI3K = Phosphatidyl Inositol 3 Kinase, ROS = Reactive Oxygen Species, PLC = Phospholipase C, PKC = Protein Kinase C, PKM2 = Pyruvate Kinase isoenzyme M2, PDH = Pyruvate Dehydrogenase, ERK = Extracellular Regulatory Kinase. The circled letter P denotes phosphorylation and activation.

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Schematic diagrams of α1 Na/K-ATPase-mediated Src regulation in normal and cancer cells. Established signaling pathways are denoted by solid black arrows and speculated signaling pathways are denoted by broken black arrows. NKA = Na/K-ATPase, Cav = Caveolin 1, FAK = Focal Adhesion Kinase, RTK = Receptor Tyrosine Kinase, PI3K = Phosphatidyl Inositol 3 Kinase, ROS = Reactive Oxygen Species, PLC = Phospholipase C, PKC = Protein Kinase C, PKM2 = Pyruvate Kinase isoenzyme M2, PDH = Pyruvate Dehydrogenase, ERK = Extracellular Regulatory Kinase. The circled letter P denotes phosphorylation and activation.

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Planar Chromatography, Activation Assay

    Y260 phosphorylation and α1 Na/K-ATPase/Src interaction in human cancers- (a) Measurement of Y260 phosphorylation in cancer cell lines. Cell lysates from a panel of prostate (LNCAP, DU145, and PC3) and breast (MCF7, MDAMB231, and BT-20) cancer cell lines were compared with corresponding control cells (prostate RWPE1 and breast MCF10A), for Na/K-ATPase α1 expression and Y260 phosphorylation. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Y260 phosphorylation and α1 Na/K-ATPase/Src interaction in human cancers- (a) Measurement of Y260 phosphorylation in cancer cell lines. Cell lysates from a panel of prostate (LNCAP, DU145, and PC3) and breast (MCF7, MDAMB231, and BT-20) cancer cell lines were compared with corresponding control cells (prostate RWPE1 and breast MCF10A), for Na/K-ATPase α1 expression and Y260 phosphorylation. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Expressing

    Identification of Y260 in α1 Na/K-ATPase as the major Src binding site- (a) Comparison of Y260 containing sequences in second cytoplasmic domain (CD2) of different human Na/K-ATPase isoforms. (b) Interaction between CD2 and Src in different cell lines. Representative blots are shown, n = 3. (c) Effects of ouabain on ERK activation. *p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Identification of Y260 in α1 Na/K-ATPase as the major Src binding site- (a) Comparison of Y260 containing sequences in second cytoplasmic domain (CD2) of different human Na/K-ATPase isoforms. (b) Interaction between CD2 and Src in different cell lines. Representative blots are shown, n = 3. (c) Effects of ouabain on ERK activation. *p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Binding Assay, Activation Assay

    Measurement of α1 Na/K-ATPase expression in primary tumor and metastatic lesions- (a) The expression of α1 Na/K-ATPase in prostate cancer. Left panels show α1 expression patterns in paired human normal prostate tissue (left), carcinoma (middle) and bone metastasis (right). Human tissue arrays were immunostained with a α1 monoclonal antibody (in brown). Hematoxylin was used for counterstaining of cell nucleus (in blue). Quantitative data are shown on the right side. **p

    Journal: Scientific Reports

    Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

    doi: 10.1038/s41598-018-29995-2

    Figure Lengend Snippet: Measurement of α1 Na/K-ATPase expression in primary tumor and metastatic lesions- (a) The expression of α1 Na/K-ATPase in prostate cancer. Left panels show α1 expression patterns in paired human normal prostate tissue (left), carcinoma (middle) and bone metastasis (right). Human tissue arrays were immunostained with a α1 monoclonal antibody (in brown). Hematoxylin was used for counterstaining of cell nucleus (in blue). Quantitative data are shown on the right side. **p

    Article Snippet: Monoclonal anti-Src (GD-11) antibody, polyclonal rabbit anti-α1 Na/K-ATPase (06-520), Protein-G-agarose beads for immunoprecipitation and PP2 -Millipore (Billerica, MA).

    Techniques: Expressing

    Western blot analysis of the specificity of NKAα1 and α3 antibodies. Brain and spinal cord homogenates were probed with antibodies raised against either NKAα1 or NKAα3. Both antibodies recognized a single band in all homogenates.

    Journal: The Journal of Neuroscience

    Article Title: Na+/K+ ATPase ?1 and ?3 Isoforms Are Differentially Expressed in ?- and ?-Motoneurons

    doi: 10.1523/JNEUROSCI.5584-12.2013

    Figure Lengend Snippet: Western blot analysis of the specificity of NKAα1 and α3 antibodies. Brain and spinal cord homogenates were probed with antibodies raised against either NKAα1 or NKAα3. Both antibodies recognized a single band in all homogenates.

    Article Snippet: Tissue sections were incubated with rabbit anti-NKAα3 (1:500–1000; Millipore Biotechnology), or mouse anti-NKAα3 (1:1000; Affinity Bioreagents) and detected using donkey anti-rabbit Alexa Fluor 555 (1:1000, Invitrogen) or donkey anti-mouse Alexa Fluor 488 (1:1000, Invitrogen) as appropriate.

    Techniques: Western Blot

    NKAα3 immunoreactive neurons can be observed throughout the spinal cord and in a subpopulation of DRG neurons. NKAα3 immunoreactivity is more ubiquitous than NKAα1-IR, with immunoreactivity throughout the neuropil at all levels

    Journal: The Journal of Neuroscience

    Article Title: Na+/K+ ATPase ?1 and ?3 Isoforms Are Differentially Expressed in ?- and ?-Motoneurons

    doi: 10.1523/JNEUROSCI.5584-12.2013

    Figure Lengend Snippet: NKAα3 immunoreactive neurons can be observed throughout the spinal cord and in a subpopulation of DRG neurons. NKAα3 immunoreactivity is more ubiquitous than NKAα1-IR, with immunoreactivity throughout the neuropil at all levels

    Article Snippet: Tissue sections were incubated with rabbit anti-NKAα3 (1:500–1000; Millipore Biotechnology), or mouse anti-NKAα3 (1:1000; Affinity Bioreagents) and detected using donkey anti-rabbit Alexa Fluor 555 (1:1000, Invitrogen) or donkey anti-mouse Alexa Fluor 488 (1:1000, Invitrogen) as appropriate.

    Techniques:

    NKAα3 is found in small γ-motoneurons, while larger α-motoneurons express NKAα1. Immunoreactivity for NKAα1 and NKAα3 reveals two distinct populations of motoneurons within the ventral horn ( A–A5

    Journal: The Journal of Neuroscience

    Article Title: Na+/K+ ATPase ?1 and ?3 Isoforms Are Differentially Expressed in ?- and ?-Motoneurons

    doi: 10.1523/JNEUROSCI.5584-12.2013

    Figure Lengend Snippet: NKAα3 is found in small γ-motoneurons, while larger α-motoneurons express NKAα1. Immunoreactivity for NKAα1 and NKAα3 reveals two distinct populations of motoneurons within the ventral horn ( A–A5

    Article Snippet: Tissue sections were incubated with rabbit anti-NKAα3 (1:500–1000; Millipore Biotechnology), or mouse anti-NKAα3 (1:1000; Affinity Bioreagents) and detected using donkey anti-rabbit Alexa Fluor 555 (1:1000, Invitrogen) or donkey anti-mouse Alexa Fluor 488 (1:1000, Invitrogen) as appropriate.

    Techniques: