atp  (Thermo Fisher)


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    Name:
    ATP
    Description:
    ATP CTP GTP UTP nucleoside 5 triphosphates and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6 T3 and T7 RNA polymerases for production of RNA probes or RNA transcripts for translation in in vitro translation extracts These nucleotides are supplied as convenient ready to use solutions at a concentration of 10 mM in 1 mM Tris HCl pH 7 5
    Catalog Number:
    18330019
    Price:
    None
    Applications:
    In Vitro Transcription|PCR & Real-Time PCR|Gene Expression Analysis & Genotyping
    Category:
    Oligos Primers Probes Nucleotides
    Buy from Supplier


    Structured Review

    Thermo Fisher atp
    ATP CTP GTP UTP nucleoside 5 triphosphates and 10 mM NTP Mix are suitable for use in in vitro transcription reactions with SP6 T3 and T7 RNA polymerases for production of RNA probes or RNA transcripts for translation in in vitro translation extracts These nucleotides are supplied as convenient ready to use solutions at a concentration of 10 mM in 1 mM Tris HCl pH 7 5
    https://www.bioz.com/result/atp/product/Thermo Fisher
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    atp - by Bioz Stars, 2020-01
    97/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Calcium Channel Types with Distinct Presynaptic Localization Couple Differentially to Transmitter Release in Single Calyx-Type Synapses
    Article Snippet: Whole-cell current-clamp recordings from terminals were made with an AxoClamp-2B (Axon Instruments, Foster City, CA) amplifier and glass pipettes (8–12 MΩ) containing (in m m ): 115 potassium gluconate, 20 KCl, 4 MgATP, 10 Na2 -phosphocreatine, 0.3 GTP, 10 HEPES, and 0.05 fura-2 (Molecular Probes, Eugene, OR), pH 7.2, adjusted with KOH. .. Apart from serving as a Ca2+ indicator, the fura-2 fluorescence was also used at the end of the experiment to confirm the presynaptic origin of the recording.

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  • 90
    Thermo Fisher bodipy fl atp
    Single-molecule binding assay of AK and <t>ATP.</t> (A) Typical fluorescence trajectory of <t>BODIPY</t> FL-ATP associating and dissociating with AK. Association and dissociation are indicated by red and green bands, respectively. (B) Evolution of the mean intensity of BODIPY FL-ATP with time. By fitting with single exponential decay, we obtain the bleaching rate constant k 2 = 0.22 ±0.01 s −1 . (C,D) Histograms of t off and t on event durations. By fitting with distribution shown in Equation (1) and (2), we obtain the association rate constant k 1 = 1.5 ± 0.2 μM −1 s −1 and the dissociation rate constant k −1 = 6.9 ± 0.7 s −1 .
    Bodipy Fl Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bodipy fl atp/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bodipy fl atp - by Bioz Stars, 2020-01
    90/100 stars
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    90
    Thermo Fisher atp solution
    DNA polymerase and nucleoside kinase activities on modified nucleosides a , MS confirmation of 5hmdC, 5fdC and <t>5cadC</t> in the purchased nucleosides. b , HPLC-UV chromatogram of nucleosides from DNA extracted from H1299 cells transfected with 5hmdCTP. The abundance of 5hmdC relative to dG is illustrated in the right panel (n=3, standard deviation is shown, n.d.= not detected). c , Coomassie-stained SDS-PAGE gel of recombinant purified DCK and CMPK1 enzymes used in the study. d , Two-dimensional TLC images of DCK reaction products. Dotted lines indicate reference points, which aid in tracking the migration localisation of the nucleosides. The monophosphate in each reaction is circled in red (representative picture, n=3). e , schematic map of nucleoside migration on two dimensional TLC plate (* indicates a background spot coming from <t>ATP</t> and used as a reference point)
    Atp Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp solution/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    atp solution - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    N/A
    MNAT1 Polyclonal Antibody for Western Blot IHC P
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    Image Search Results


    Single-molecule binding assay of AK and ATP. (A) Typical fluorescence trajectory of BODIPY FL-ATP associating and dissociating with AK. Association and dissociation are indicated by red and green bands, respectively. (B) Evolution of the mean intensity of BODIPY FL-ATP with time. By fitting with single exponential decay, we obtain the bleaching rate constant k 2 = 0.22 ±0.01 s −1 . (C,D) Histograms of t off and t on event durations. By fitting with distribution shown in Equation (1) and (2), we obtain the association rate constant k 1 = 1.5 ± 0.2 μM −1 s −1 and the dissociation rate constant k −1 = 6.9 ± 0.7 s −1 .

    Journal: Frontiers in Plant Science

    Article Title: Single-Molecule Fluorescence Methods to Study Plant Hormone Signal Transduction Pathways

    doi: 10.3389/fpls.2017.01888

    Figure Lengend Snippet: Single-molecule binding assay of AK and ATP. (A) Typical fluorescence trajectory of BODIPY FL-ATP associating and dissociating with AK. Association and dissociation are indicated by red and green bands, respectively. (B) Evolution of the mean intensity of BODIPY FL-ATP with time. By fitting with single exponential decay, we obtain the bleaching rate constant k 2 = 0.22 ±0.01 s −1 . (C,D) Histograms of t off and t on event durations. By fitting with distribution shown in Equation (1) and (2), we obtain the association rate constant k 1 = 1.5 ± 0.2 μM −1 s −1 and the dissociation rate constant k −1 = 6.9 ± 0.7 s −1 .

    Article Snippet: BODIPY-FL-ATP was purchased from Thermo Fisher Scientific (A12410, America).

    Techniques: Binding Assay, Fluorescence

    DNA polymerase and nucleoside kinase activities on modified nucleosides a , MS confirmation of 5hmdC, 5fdC and 5cadC in the purchased nucleosides. b , HPLC-UV chromatogram of nucleosides from DNA extracted from H1299 cells transfected with 5hmdCTP. The abundance of 5hmdC relative to dG is illustrated in the right panel (n=3, standard deviation is shown, n.d.= not detected). c , Coomassie-stained SDS-PAGE gel of recombinant purified DCK and CMPK1 enzymes used in the study. d , Two-dimensional TLC images of DCK reaction products. Dotted lines indicate reference points, which aid in tracking the migration localisation of the nucleosides. The monophosphate in each reaction is circled in red (representative picture, n=3). e , schematic map of nucleoside migration on two dimensional TLC plate (* indicates a background spot coming from ATP and used as a reference point)

    Journal: Nature

    Article Title: CDA directs metabolism of epigenetic nucleosides revealing a therapeutic window in cancer

    doi: 10.1038/nature14948

    Figure Lengend Snippet: DNA polymerase and nucleoside kinase activities on modified nucleosides a , MS confirmation of 5hmdC, 5fdC and 5cadC in the purchased nucleosides. b , HPLC-UV chromatogram of nucleosides from DNA extracted from H1299 cells transfected with 5hmdCTP. The abundance of 5hmdC relative to dG is illustrated in the right panel (n=3, standard deviation is shown, n.d.= not detected). c , Coomassie-stained SDS-PAGE gel of recombinant purified DCK and CMPK1 enzymes used in the study. d , Two-dimensional TLC images of DCK reaction products. Dotted lines indicate reference points, which aid in tracking the migration localisation of the nucleosides. The monophosphate in each reaction is circled in red (representative picture, n=3). e , schematic map of nucleoside migration on two dimensional TLC plate (* indicates a background spot coming from ATP and used as a reference point)

    Article Snippet: Nucleoside stability Nucleosides were obtained from the following sources: 5hmdC (PY-7588, Berry & Associates), 5fdC (PY-7589, Berry & Associates), 5cadC (PY-7593, Berry & Associates), 5AZAdC (A3656, Sigma Aldrich), ATP solution (Thermo Fisher), [γ-32P] ATP (Perkin Elmer), dC (Sigma Aldrich, D3897), dCMP (Sigma Aldrich, D7625), 5hmdCTP (Bioline, BIO-39046).

    Techniques: Modification, Mass Spectrometry, High Performance Liquid Chromatography, Transfection, Standard Deviation, Staining, SDS Page, Recombinant, Purification, Thin Layer Chromatography, Migration

    Anti-IAV activities of ABT-263 analogues. ( A , B ) Structures of ABT-263, ABT-737, and ABT-199, as well as WEHI-539, A-1331852, and A-1155463, showing that these molecules share similar elements; ( C ) fluorescent microscopy images showing that increasing concentrations of Bcl-2i kill IAV-infected (moi 3) but not mock-infected RPE cells at 24 hpi. Asymmetric cyanine dye stains the dsDNA of dead cells. Hoechst stains DNA in living cells; ( D ) quantification of dsDNA in dead cells. Mean ± SD, n = 3; ( E ) quantification of intracellular ATP in living cells using CTG assay. Mean ± SD, n = 3.

    Journal: Viruses

    Article Title: Antiviral Properties of Chemical Inhibitors of Cellular Anti-Apoptotic Bcl-2 Proteins

    doi: 10.3390/v9100271

    Figure Lengend Snippet: Anti-IAV activities of ABT-263 analogues. ( A , B ) Structures of ABT-263, ABT-737, and ABT-199, as well as WEHI-539, A-1331852, and A-1155463, showing that these molecules share similar elements; ( C ) fluorescent microscopy images showing that increasing concentrations of Bcl-2i kill IAV-infected (moi 3) but not mock-infected RPE cells at 24 hpi. Asymmetric cyanine dye stains the dsDNA of dead cells. Hoechst stains DNA in living cells; ( D ) quantification of dsDNA in dead cells. Mean ± SD, n = 3; ( E ) quantification of intracellular ATP in living cells using CTG assay. Mean ± SD, n = 3.

    Article Snippet: Hoechst 33342 (20 mM solution; cat #: 62249) and ATP (10 mM solution; cat #: PV3227) were from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Microscopy, Infection, CTG Assay

    At 24 h post infection, ABT-263 kills influenza A (IAV)-infected but not mock-infected RPE cells and lowers the production of infectious viral particles. ( A ) Fluorescent microscopy images showing that increasing concentrations of ABT-263 kill IAV-infected (moi 3) but not mock-infected retinal pigment epithelium (RPE) cells at 24 h. Asymmetric cyanine dye stains the dsDNA of dead cells. Hoechst stains DNA in living cells; ( B ) quantification of dsDNA in dead cells using CellToxGreen cytotoxicity (CTxG) assay. Mean ± standard deviation (SD), n = 3; ( C ) quantification of intracellular ATP in living cells using CellTiter-Glo luminescent cell viability (CTG) assay. Mean ± standard deviation (SD), n = 3; ( D ) RPE cells were non- or ABT-263-treated (0.4 μM) and infected with IAV at moi 0.08, 0.4, 2, and 10. Cell viability was measured using a CTG assay 24 h after infection. Mean ± SD, n = 3; ( E ) RPE cells were non- or ABT-263-treated (0.4 μM) and mock- or IAV-infected (moi 3), and cell viability was measured using a CTG assay at the indicated time points. Mean ± SD, n = 3; ( F ) example of plaque assay measuring virus production in Bcl-2i- (3 µM) and DMSO-treated RPE cells at 24 hpi; ( G ) table summarizing the differential effect of ABT-263 on the viability of virus- and mock-infected cells, expressed as ΔAUC CxTG and ΔAUC CTG . It also shows the effect of ABT-263 on virus production in drug- (3 µM) and DMSO-treated RPE cells, which is expressed in log 10 fold change (FC). Mean ± SD, n = 3.

    Journal: Viruses

    Article Title: Antiviral Properties of Chemical Inhibitors of Cellular Anti-Apoptotic Bcl-2 Proteins

    doi: 10.3390/v9100271

    Figure Lengend Snippet: At 24 h post infection, ABT-263 kills influenza A (IAV)-infected but not mock-infected RPE cells and lowers the production of infectious viral particles. ( A ) Fluorescent microscopy images showing that increasing concentrations of ABT-263 kill IAV-infected (moi 3) but not mock-infected retinal pigment epithelium (RPE) cells at 24 h. Asymmetric cyanine dye stains the dsDNA of dead cells. Hoechst stains DNA in living cells; ( B ) quantification of dsDNA in dead cells using CellToxGreen cytotoxicity (CTxG) assay. Mean ± standard deviation (SD), n = 3; ( C ) quantification of intracellular ATP in living cells using CellTiter-Glo luminescent cell viability (CTG) assay. Mean ± standard deviation (SD), n = 3; ( D ) RPE cells were non- or ABT-263-treated (0.4 μM) and infected with IAV at moi 0.08, 0.4, 2, and 10. Cell viability was measured using a CTG assay 24 h after infection. Mean ± SD, n = 3; ( E ) RPE cells were non- or ABT-263-treated (0.4 μM) and mock- or IAV-infected (moi 3), and cell viability was measured using a CTG assay at the indicated time points. Mean ± SD, n = 3; ( F ) example of plaque assay measuring virus production in Bcl-2i- (3 µM) and DMSO-treated RPE cells at 24 hpi; ( G ) table summarizing the differential effect of ABT-263 on the viability of virus- and mock-infected cells, expressed as ΔAUC CxTG and ΔAUC CTG . It also shows the effect of ABT-263 on virus production in drug- (3 µM) and DMSO-treated RPE cells, which is expressed in log 10 fold change (FC). Mean ± SD, n = 3.

    Article Snippet: Hoechst 33342 (20 mM solution; cat #: 62249) and ATP (10 mM solution; cat #: PV3227) were from Thermo Fisher Scientific (Waltham, MA, USA).

    Techniques: Infection, Microscopy, Standard Deviation, CTG Assay, Plaque Assay