atp glo bioluminometric cell viability assay  (Biotium)

 
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    Biotium atp glo bioluminometric cell viability assay
    Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) <t>ATP</t> content was assessed by <t>ATP-Glo</t> <t>Bioluminometric</t> Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P
    Atp Glo Bioluminometric Cell Viability Assay, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp glo bioluminometric cell viability assay/product/Biotium
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp glo bioluminometric cell viability assay - by Bioz Stars, 2020-05
    88/100 stars

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    1) Product Images from "Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development"

    Article Title: Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2017.453

    Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) ATP content was assessed by ATP-Glo Bioluminometric Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P
    Figure Legend Snippet: Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) ATP content was assessed by ATP-Glo Bioluminometric Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P

    Techniques Used: Staining, Viability Assay, Immunofluorescence, Labeling, Transmission Electron Microscopy

    Related Articles

    ATP Assay:

    Article Title: Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development
    Article Snippet: .. ATP Assay The ATP-Glo Bioluminometric Cell Viability Assay (Biotium, Hayward, CA, USA) was used to assess cellular ATP levels according to the manufacturer’s protocol. .. TUNEL Chondrocytes or cartilage sections were fixed and then stained with in situ cell death detection kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions for 30 min at 37 °C and the nuclei was stained with DAPI.

    Viability Assay:

    Article Title: Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development
    Article Snippet: .. ATP Assay The ATP-Glo Bioluminometric Cell Viability Assay (Biotium, Hayward, CA, USA) was used to assess cellular ATP levels according to the manufacturer’s protocol. .. TUNEL Chondrocytes or cartilage sections were fixed and then stained with in situ cell death detection kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions for 30 min at 37 °C and the nuclei was stained with DAPI.

    Article Title: Lemur Tyrosine Kinase 2, a novel target in prostate cancer therapy
    Article Snippet: .. We further tested the effects of a decrease in LMTK2 expression on prostate cancer cell viability using the ATP-Glo Bioluminometric cell viability assay (Biotium, CA). ..

    Expressing:

    Article Title: Lemur Tyrosine Kinase 2, a novel target in prostate cancer therapy
    Article Snippet: .. We further tested the effects of a decrease in LMTK2 expression on prostate cancer cell viability using the ATP-Glo Bioluminometric cell viability assay (Biotium, CA). ..

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  • 88
    Biotium atp glo bioluminometric cell viability assay
    Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) <t>ATP</t> content was assessed by <t>ATP-Glo</t> <t>Bioluminometric</t> Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P
    Atp Glo Bioluminometric Cell Viability Assay, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp glo bioluminometric cell viability assay/product/Biotium
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp glo bioluminometric cell viability assay - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) ATP content was assessed by ATP-Glo Bioluminometric Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P

    Journal: Cell Death & Disease

    Article Title: Trehalose ameliorates oxidative stress-mediated mitochondrial dysfunction and ER stress via selective autophagy stimulation and autophagic flux restoration in osteoarthritis development

    doi: 10.1038/cddis.2017.453

    Figure Lengend Snippet: Trehalose promotes autophagic flux and attenuates mitochondrial dysfunction in chondrocytes. ( a and b ) Mitochondrial membrane potential was detected using Mitotracker and the nuclei were stained with Hoechst. (bar: 10 μ m). ( c ) ATP content was assessed by ATP-Glo Bioluminometric Cell Viability Assay. ( d ) Immunofluorescence double-labeled staining for co-localization of Drp-1 with Tom20 (Green: drp-1, red: Tom 20, bar: 10 μ m). ( e ) TEM images of the mitochondria and autophaghic change in chondrocytes (× 15 000 or 50 000). Lightning mark: mitochondrial fission; Asterisk: swollen mitochondira with fractured cristae; Double arrow: autophagosome with double membrane structure; single arrow: autophagolysosome). ( f and g ) The protein levels of SOD2, Bax, Bcl-2 and Cyt C in chondrocytes treated as indicated. ( h and i ) Double-labeled immunofluorescence staining of p62 and Cyt C in chondrocytes. (Green: p62, red: Cyt C, bar: 10 μ m). All data represent mean±S.D ( n =5). ** P

    Article Snippet: ATP Assay The ATP-Glo Bioluminometric Cell Viability Assay (Biotium, Hayward, CA, USA) was used to assess cellular ATP levels according to the manufacturer’s protocol.

    Techniques: Staining, Viability Assay, Immunofluorescence, Labeling, Transmission Electron Microscopy