atp depleted adp  (Millipore)


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  • 93
    Name:
    Sodium fluoride 0 5 M solution
    Description:

    Catalog Number:
    67414
    Price:
    None
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    Structured Review

    Millipore atp depleted adp
    Sodium fluoride 0 5 M solution

    https://www.bioz.com/result/atp depleted adp/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp depleted adp - by Bioz Stars, 2020-09
    93/100 stars

    Related Products / Commonly Used Together

    \ usepackage -lcb-
    alcl3
    adp ·

    Images

    1) Product Images from "RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis"

    Article Title: RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw881

    Model for ATP-dependent G4 destabilization by RHAU. RHAU (represented by its two RecA-like domains, the conserved C-terminal domain and the N-terminal RSM domain) binds to the G4 substrate in both (i) nucleotide-free and (ii) ATP-bound states. G4 destabilization occurs during (iii and iv) ATP hydrolysis and the following ADP or phosphate release steps. The RHAU–ssDNA binding interface is drawn based on the structure of a closely related DEAH-box helicase ( 29 ). The binding interface of RSM domain with G4 is drawn based on a recent NMR-solved structure ( 26 ).
    Figure Legend Snippet: Model for ATP-dependent G4 destabilization by RHAU. RHAU (represented by its two RecA-like domains, the conserved C-terminal domain and the N-terminal RSM domain) binds to the G4 substrate in both (i) nucleotide-free and (ii) ATP-bound states. G4 destabilization occurs during (iii and iv) ATP hydrolysis and the following ADP or phosphate release steps. The RHAU–ssDNA binding interface is drawn based on the structure of a closely related DEAH-box helicase ( 29 ). The binding interface of RSM domain with G4 is drawn based on a recent NMR-solved structure ( 26 ).

    Techniques Used: Binding Assay, Nuclear Magnetic Resonance

    Related Articles

    Ubiquitin Assay:

    Article Title: Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation
    Article Snippet: .. For reagents used for in vitro ubiquitination assay, Creatine kinase (C3755), Creatine phosphate (27920), Adenosine 5′-triphosphate disodium salt hydrate (A2383) and Sodium fluoride (67414) were purchased from Sigma-Aldrich; HA-ubiquitin (U-110), Ubiquitin aldehyde (Ub-H) (U-201), UBE1/ubiquitin activating enzyme (E-305) and UBE2D1/UbcH5a (E2-616) were purchased from Boston Biochem. .. HEK293T (CRL-3216) and HeLa (CCL-2) cells were purchased from ATCC. siRNA targeting CUL4A #1 (5ʹ UCCUGCAUUAACCUUUGG 3ʹ, used in this study [ ]) and CUL4A #2 (5ʹ AUCUUCAUUAUUCUGACG 3ʹ, used in this study [ ]) was a gift from Dr. Thilo Hagen from National University of Singapore.

    In Vitro:

    Article Title: Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation
    Article Snippet: .. For reagents used for in vitro ubiquitination assay, Creatine kinase (C3755), Creatine phosphate (27920), Adenosine 5′-triphosphate disodium salt hydrate (A2383) and Sodium fluoride (67414) were purchased from Sigma-Aldrich; HA-ubiquitin (U-110), Ubiquitin aldehyde (Ub-H) (U-201), UBE1/ubiquitin activating enzyme (E-305) and UBE2D1/UbcH5a (E2-616) were purchased from Boston Biochem. .. HEK293T (CRL-3216) and HeLa (CCL-2) cells were purchased from ATCC. siRNA targeting CUL4A #1 (5ʹ UCCUGCAUUAACCUUUGG 3ʹ, used in this study [ ]) and CUL4A #2 (5ʹ AUCUUCAUUAUUCUGACG 3ʹ, used in this study [ ]) was a gift from Dr. Thilo Hagen from National University of Singapore.

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  • 91
    Millipore atp species
    Fluorescence anisotropy measurements (Δ r ) of equilibrium binding of ISWI to DNA and nucleosomes in the presence of nucleotides. (A) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of <t>ATP-γ-S.</t> These data were analyzed using Scheme 1 as described in Experimental Procedures . The solid lines in the figure represent the fits of the data to this scheme, which returned the following values: 1/β A = 140 ± 30 μM, 1/β A,1 = 390 ± 70 μM, and 1/β 1,A = 42 ± 8 nM. (B) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of <t>ADP.</t> The solid line in this figure represents the fit of equilibrium DNA binding data collected in the absence of nucleotide (Figure 1 A). (C) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ATP-γ-S. (D) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ADP. The solid lines in panels C and D are the fits of the equilibrium nucleosome binding data collected in the absence of nucleotides (Figure 1 C).
    Atp Species, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp species/product/Millipore
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    atp species - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    99
    Millipore adp atp exchange assay
    <t>ADP/ATP</t> exchange assays with human ANT isoforms expressed in isolated yeast mitochondria and Lactococcus lactis. ( A for description). ( B ) Assay validation with known compounds (10 μM) in isolated yeast mitochondria (left panel) and L. lactis (right panel) using the hANT2 isoform. The ADP/ATP exchange activity was expressed as the percent relative to the DMSO control. Mean ± SD from a single experiment. ANT, adenine nucleotide translocase; TTFA, thenoyltrifluoroacetone; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; MPTP, mitochondrial permeability transition pore
    Adp Atp Exchange Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adp atp exchange assay/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    adp atp exchange assay - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Fluorescence anisotropy measurements (Δ r ) of equilibrium binding of ISWI to DNA and nucleosomes in the presence of nucleotides. (A) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of ATP-γ-S. These data were analyzed using Scheme 1 as described in Experimental Procedures . The solid lines in the figure represent the fits of the data to this scheme, which returned the following values: 1/β A = 140 ± 30 μM, 1/β A,1 = 390 ± 70 μM, and 1/β 1,A = 42 ± 8 nM. (B) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of ADP. The solid line in this figure represents the fit of equilibrium DNA binding data collected in the absence of nucleotide (Figure 1 A). (C) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ATP-γ-S. (D) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ADP. The solid lines in panels C and D are the fits of the equilibrium nucleosome binding data collected in the absence of nucleotides (Figure 1 C).

    Journal: Biochemistry

    Article Title: Quantitative Determination of Binding of ISWI to Nucleosomes and DNA Shows Allosteric Regulation of DNA Binding by Nucleotides

    doi: 10.1021/bi500224t

    Figure Lengend Snippet: Fluorescence anisotropy measurements (Δ r ) of equilibrium binding of ISWI to DNA and nucleosomes in the presence of nucleotides. (A) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of ATP-γ-S. These data were analyzed using Scheme 1 as described in Experimental Procedures . The solid lines in the figure represent the fits of the data to this scheme, which returned the following values: 1/β A = 140 ± 30 μM, 1/β A,1 = 390 ± 70 μM, and 1/β 1,A = 42 ± 8 nM. (B) Equilibrium binding to a 20 bp FITC-labeled DNA substrate (25 nM) in the presence of ADP. The solid line in this figure represents the fit of equilibrium DNA binding data collected in the absence of nucleotide (Figure 1 A). (C) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ATP-γ-S. (D) Equilibrium binding to an Alexa488-labeled 10N5 nucleosome substrate in the presence of ADP. The solid lines in panels C and D are the fits of the equilibrium nucleosome binding data collected in the absence of nucleotides (Figure 1 C).

    Article Snippet: To separate ADP from ATP species, reaction mixtures were analyzed using thin liquid chromatography PEI-cellulose plates (EMD chemicals) in 0.6 M potassium phosphate (pH 3.4) buffer, quantified using a Typhoon Phosphor imager.

    Techniques: Fluorescence, Binding Assay, Labeling

    ADP/ATP exchange assays with human ANT isoforms expressed in isolated yeast mitochondria and Lactococcus lactis. ( A for description). ( B ) Assay validation with known compounds (10 μM) in isolated yeast mitochondria (left panel) and L. lactis (right panel) using the hANT2 isoform. The ADP/ATP exchange activity was expressed as the percent relative to the DMSO control. Mean ± SD from a single experiment. ANT, adenine nucleotide translocase; TTFA, thenoyltrifluoroacetone; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; MPTP, mitochondrial permeability transition pore

    Journal: Journal of biomolecular screening

    Article Title: Human Adenine Nucleotide Translocase (ANT) Modulators Identified by High-Throughput Screening of Transgenic Yeast

    doi: 10.1177/1087057115624637

    Figure Lengend Snippet: ADP/ATP exchange assays with human ANT isoforms expressed in isolated yeast mitochondria and Lactococcus lactis. ( A for description). ( B ) Assay validation with known compounds (10 μM) in isolated yeast mitochondria (left panel) and L. lactis (right panel) using the hANT2 isoform. The ADP/ATP exchange activity was expressed as the percent relative to the DMSO control. Mean ± SD from a single experiment. ANT, adenine nucleotide translocase; TTFA, thenoyltrifluoroacetone; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; MPTP, mitochondrial permeability transition pore

    Article Snippet: The ADP/ATP exchange assay was carried out in a filter plate format using a 96-well MultiScreenHTS FC filter plate (Millipore, Billerica, MA).

    Techniques: Isolation, Activity Assay, Permeability

    Characterization of Ant2 -deleted liver mitochondria. ( a ) ADP/ATP exchange capacity measured by [ 3 H]ADP uptake in the presence or absence of the ANT specific inhibitor, carboxyatractyloside (CATR). Left two panels show representative data from control and Ant2 cKO liver mitochondria, while the far right graph shows the average of multiple experiments ( n =5; ** P

    Journal: Nature Communications

    Article Title: Mitochondrial ATP transporter depletion protects mice against liver steatosis and insulin resistance

    doi: 10.1038/ncomms14477

    Figure Lengend Snippet: Characterization of Ant2 -deleted liver mitochondria. ( a ) ADP/ATP exchange capacity measured by [ 3 H]ADP uptake in the presence or absence of the ANT specific inhibitor, carboxyatractyloside (CATR). Left two panels show representative data from control and Ant2 cKO liver mitochondria, while the far right graph shows the average of multiple experiments ( n =5; ** P

    Article Snippet: ADP/ATP exchange assay The ADP/ATP exchange assay was performed on a filter-plate (glassfiber FB, Millipore).

    Techniques: