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Promega atp celltitre glo
Atp Celltitre Glo, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp celltitre glo/product/Promega
Average 85 stars, based on 1 article reviews
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atp celltitre glo - by Bioz Stars, 2020-04
85/100 stars

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ATP Assay:

Article Title: Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line
Article Snippet: .. ATP assay Cells (20,000/well in six replicates) were aliquoted in an opaque 96-well microtitre plate to which the ATP CellTitre Glo (Promega, Madison, USA) reagent (50 μl) was added and allowed to react in the dark (30 min, RT). .. After incubation, the luminescent signal proportional to the cellular ATP content was detected with a Modulus™ microplate reader (Turner Biosystems, Sunnyvale, USA).

Incubation:

Article Title: Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line
Article Snippet: ATP assay Cells (20,000/well in six replicates) were aliquoted in an opaque 96-well microtitre plate to which the ATP CellTitre Glo (Promega, Madison, USA) reagent (50 μl) was added and allowed to react in the dark (30 min, RT). .. After incubation, the luminescent signal proportional to the cellular ATP content was detected with a Modulus™ microplate reader (Turner Biosystems, Sunnyvale, USA).

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  • 97
    Promega atp level
    Kaempferol rescues cells against BFA induced cell death. ( a ) Screening for cytoprotective compounds (50 µM) against BFA (1 µg/ml) induced ER stress in IMR32 cell line. Cells were plated and pre-incubated with the test compounds (90 min) and treated with ER stress inducer BFA, incubated for 24 h. Cell viability was estimated by MTT assay. BFA: Brefeldin A; Withan: Withanone; With A: Withanaloide A; With B: Withanaloide B; kaemp: kaempferol; kaempG: Kaempfeol-3-O-robinbioside-7-O-glucoside; Bacop I: Bacopaside I; PEA: Palmitoylethanolamide; PBA: Phenyl Butyric acid; Sal: Salubrinal. ( b ) Confirmation of cytoprotective activity of kaempferol by measuring cellular <t>ATP</t> level using CellTitre <t>Glo</t> reagent. ( c ) Phase contrast images of IMR32 cells after BFA treatment in presence or absence of kaempferol pre-incubation. ( d–f ) Cytoprotective activity of kaempferol against BFA induced cell death in multiple cell lines with incubation time of 24 hours. Cell viability was assessed using MTT assay. ( g ) Inhibition of caspase 3/7 activation by kaempferol against BFA and CDDO-Me induced caspase 3/7 activation in IMR32 cells. Data represents mean ± SEM of experiment performed in triplicate (n = 3). Note: *Represents the significance between cell death inducer alone treated condition compared to kaempferol pre-treated condition, at p ≤ 0.05 (one way ANOVA).
    Atp Level, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp level/product/Promega
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp level - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    92
    Promega atp based luminescence assay kit
    BNTX sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis (A) Assay data for a screening analysis of AsPC-1 cells pre-incubated with various test compounds (5 μM) and were then exposed to TRAIL (50 ng/ml). Cell survival was measured as a percentage of the control sample. The arrow indicates BNTX (5 μM). (B) AsPC-1 cells were exposed to various concentrations of BNTX with or without TRAIL (25 ng/ml) for 24 h. Cell viability was measured by a luminescent <t>CellTiter-Glo</t> assay, and relative cell survival was determined by assaying <t>ATP</t> levels. (C) Relative survival of three pancreatic cancer cell lines (AsPC-1, PANC-1, and MIA PaCa-2) exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cell viability was measured by a luminescent CellTiter-Glo assay. (D) Apoptotic activity in AsPC-1 cells exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cells were analyzed by annexin V/PI double-stained flow cytometry. (E) The percentage of apoptotic cells. Values are the mean ± SD from two independent experiments performed in duplicate. Asterisks indicate significant differences compared with the control ( *** P
    Atp Based Luminescence Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp based luminescence assay kit/product/Promega
    Average 92 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    atp based luminescence assay kit - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    99
    Promega atp content
    AQP1 functions as a genetically encoded reporter for diffusion-weighted MRI. ( a ) Illustration of the impact of aquaporin expression on water diffusion across the cell membrane and the resulting decrease in diffusion-weighted signal intensity. ( b ) Diffusion-weighted images of CHO, U87 and Neuro 2a cell pellets expressing AQP1 or GFP, acquired using a b -value of ∼1,000 s mm −2 . Scale bars, 3 mm. ( c ) ADC of water in CHO, U87 and Neuro 2a cells expressing AQP1 relative to GFP controls, measured at Δ eff =398 ms. Transgene expression in CHO cells was induced with 1 μg ml −1 doxycycline, whereas U87 and Neuro 2a cells express AQP1 from a constitutive promoter. n =4 (U87, Neuro 2a) and 5 (CHO) biological replicates. ( d ) Longitudinal ( T 1 ) and ( e ) transverse ( T 2 ) relaxation rates in cells expressing AQP1 or GFP. n =3 (Neuro 2a, CHO) or 4 (U87) biological replicates. ( f ) Cell viability on AQP1 or GFP expression. n =12 (resazurin assay), 6 <t>(ATP</t> content), 4 (LDH release) and 3 (ethidium staining) biological replicates. Error bars±s.e.m. ( g ) Phase-contrast images of CHO, U87 and Neuro 2a cells expressing AQP1 or GFP. Scale bars, 10 μm.
    Atp Content, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp content/product/Promega
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    atp content - by Bioz Stars, 2020-04
    99/100 stars
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    Image Search Results


    Kaempferol rescues cells against BFA induced cell death. ( a ) Screening for cytoprotective compounds (50 µM) against BFA (1 µg/ml) induced ER stress in IMR32 cell line. Cells were plated and pre-incubated with the test compounds (90 min) and treated with ER stress inducer BFA, incubated for 24 h. Cell viability was estimated by MTT assay. BFA: Brefeldin A; Withan: Withanone; With A: Withanaloide A; With B: Withanaloide B; kaemp: kaempferol; kaempG: Kaempfeol-3-O-robinbioside-7-O-glucoside; Bacop I: Bacopaside I; PEA: Palmitoylethanolamide; PBA: Phenyl Butyric acid; Sal: Salubrinal. ( b ) Confirmation of cytoprotective activity of kaempferol by measuring cellular ATP level using CellTitre Glo reagent. ( c ) Phase contrast images of IMR32 cells after BFA treatment in presence or absence of kaempferol pre-incubation. ( d–f ) Cytoprotective activity of kaempferol against BFA induced cell death in multiple cell lines with incubation time of 24 hours. Cell viability was assessed using MTT assay. ( g ) Inhibition of caspase 3/7 activation by kaempferol against BFA and CDDO-Me induced caspase 3/7 activation in IMR32 cells. Data represents mean ± SEM of experiment performed in triplicate (n = 3). Note: *Represents the significance between cell death inducer alone treated condition compared to kaempferol pre-treated condition, at p ≤ 0.05 (one way ANOVA).

    Journal: Scientific Reports

    Article Title: Kaempferol mitigates Endoplasmic Reticulum Stress Induced Cell Death by targeting caspase 3/7

    doi: 10.1038/s41598-018-20499-7

    Figure Lengend Snippet: Kaempferol rescues cells against BFA induced cell death. ( a ) Screening for cytoprotective compounds (50 µM) against BFA (1 µg/ml) induced ER stress in IMR32 cell line. Cells were plated and pre-incubated with the test compounds (90 min) and treated with ER stress inducer BFA, incubated for 24 h. Cell viability was estimated by MTT assay. BFA: Brefeldin A; Withan: Withanone; With A: Withanaloide A; With B: Withanaloide B; kaemp: kaempferol; kaempG: Kaempfeol-3-O-robinbioside-7-O-glucoside; Bacop I: Bacopaside I; PEA: Palmitoylethanolamide; PBA: Phenyl Butyric acid; Sal: Salubrinal. ( b ) Confirmation of cytoprotective activity of kaempferol by measuring cellular ATP level using CellTitre Glo reagent. ( c ) Phase contrast images of IMR32 cells after BFA treatment in presence or absence of kaempferol pre-incubation. ( d–f ) Cytoprotective activity of kaempferol against BFA induced cell death in multiple cell lines with incubation time of 24 hours. Cell viability was assessed using MTT assay. ( g ) Inhibition of caspase 3/7 activation by kaempferol against BFA and CDDO-Me induced caspase 3/7 activation in IMR32 cells. Data represents mean ± SEM of experiment performed in triplicate (n = 3). Note: *Represents the significance between cell death inducer alone treated condition compared to kaempferol pre-treated condition, at p ≤ 0.05 (one way ANOVA).

    Article Snippet: The cytoprotective activity of kaempferol was confirmed by an orthogonal assay that measures the ATP level (CellTiter-Glo luminescent cell viability assay, Promega, India) and trypan blue dye exclusion assay as a surrogate for the cell viability (Fig. and Supplementary Figure 4a).

    Techniques: Incubation, MTT Assay, Activity Assay, Inhibition, Activation Assay

    BNTX sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis (A) Assay data for a screening analysis of AsPC-1 cells pre-incubated with various test compounds (5 μM) and were then exposed to TRAIL (50 ng/ml). Cell survival was measured as a percentage of the control sample. The arrow indicates BNTX (5 μM). (B) AsPC-1 cells were exposed to various concentrations of BNTX with or without TRAIL (25 ng/ml) for 24 h. Cell viability was measured by a luminescent CellTiter-Glo assay, and relative cell survival was determined by assaying ATP levels. (C) Relative survival of three pancreatic cancer cell lines (AsPC-1, PANC-1, and MIA PaCa-2) exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cell viability was measured by a luminescent CellTiter-Glo assay. (D) Apoptotic activity in AsPC-1 cells exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cells were analyzed by annexin V/PI double-stained flow cytometry. (E) The percentage of apoptotic cells. Values are the mean ± SD from two independent experiments performed in duplicate. Asterisks indicate significant differences compared with the control ( *** P

    Journal: Oncotarget

    Article Title: Downregulation of X-linked inhibitor of apoptosis protein by ‘7-Benzylidenenaltrexone maleate’ sensitizes pancreatic cancer cells to TRAIL-induced apoptosis

    doi: 10.18632/oncotarget.17841

    Figure Lengend Snippet: BNTX sensitizes human pancreatic cancer cells to TRAIL-induced apoptosis (A) Assay data for a screening analysis of AsPC-1 cells pre-incubated with various test compounds (5 μM) and were then exposed to TRAIL (50 ng/ml). Cell survival was measured as a percentage of the control sample. The arrow indicates BNTX (5 μM). (B) AsPC-1 cells were exposed to various concentrations of BNTX with or without TRAIL (25 ng/ml) for 24 h. Cell viability was measured by a luminescent CellTiter-Glo assay, and relative cell survival was determined by assaying ATP levels. (C) Relative survival of three pancreatic cancer cell lines (AsPC-1, PANC-1, and MIA PaCa-2) exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cell viability was measured by a luminescent CellTiter-Glo assay. (D) Apoptotic activity in AsPC-1 cells exposed to TRAIL (25 ng/ml) with or without BNTX (2.5 μM) for 24 h. Cells were analyzed by annexin V/PI double-stained flow cytometry. (E) The percentage of apoptotic cells. Values are the mean ± SD from two independent experiments performed in duplicate. Asterisks indicate significant differences compared with the control ( *** P

    Article Snippet: Cell viability assay and flow cytometry Cells were seeded at 2∼3 × 103 cells/well in 96-well plates and were pre-treated with BNTX at indicated concentrations for 0.5 h followed by TRAIL (25 ng/ml) for an additional 24 h. Cell viability assays were performed using a cellular ATP-based luminescence assay kit (CellTiter-Glo; Promega, Madison, WI).

    Techniques: Incubation, Glo Assay, Activity Assay, Staining, Flow Cytometry, Cytometry

    Intracellular ATP levels in wild-type and CatE −/− macrophages. Macrophages (1 × 10 5 cells) were cultured on 96-well plates at 37°C for 24 h. After replacement with fresh media, the culture plate was incubated at room temperature (25°C) for 30 min. ATP levels were determined by the manufacturer’s protocol of the CellTiter-Glo assay kit according to the manufacturer’s protocol. The concentration of intracellular ATP was determined by the titration of the control medium without cells plus 0.1−1.0 µM ATP.

    Journal: PLoS ONE

    Article Title: Cathepsin E Deficiency Impairs Autophagic Proteolysis in Macrophages

    doi: 10.1371/journal.pone.0082415

    Figure Lengend Snippet: Intracellular ATP levels in wild-type and CatE −/− macrophages. Macrophages (1 × 10 5 cells) were cultured on 96-well plates at 37°C for 24 h. After replacement with fresh media, the culture plate was incubated at room temperature (25°C) for 30 min. ATP levels were determined by the manufacturer’s protocol of the CellTiter-Glo assay kit according to the manufacturer’s protocol. The concentration of intracellular ATP was determined by the titration of the control medium without cells plus 0.1−1.0 µM ATP.

    Article Snippet: The ATP assay kit (CellTiter-Gio Luminescent cell viability assay) was purchased from Promega (Madison, WI, USA).

    Techniques: Cell Culture, Incubation, Glo Assay, Concentration Assay, Titration

    AQP1 functions as a genetically encoded reporter for diffusion-weighted MRI. ( a ) Illustration of the impact of aquaporin expression on water diffusion across the cell membrane and the resulting decrease in diffusion-weighted signal intensity. ( b ) Diffusion-weighted images of CHO, U87 and Neuro 2a cell pellets expressing AQP1 or GFP, acquired using a b -value of ∼1,000 s mm −2 . Scale bars, 3 mm. ( c ) ADC of water in CHO, U87 and Neuro 2a cells expressing AQP1 relative to GFP controls, measured at Δ eff =398 ms. Transgene expression in CHO cells was induced with 1 μg ml −1 doxycycline, whereas U87 and Neuro 2a cells express AQP1 from a constitutive promoter. n =4 (U87, Neuro 2a) and 5 (CHO) biological replicates. ( d ) Longitudinal ( T 1 ) and ( e ) transverse ( T 2 ) relaxation rates in cells expressing AQP1 or GFP. n =3 (Neuro 2a, CHO) or 4 (U87) biological replicates. ( f ) Cell viability on AQP1 or GFP expression. n =12 (resazurin assay), 6 (ATP content), 4 (LDH release) and 3 (ethidium staining) biological replicates. Error bars±s.e.m. ( g ) Phase-contrast images of CHO, U87 and Neuro 2a cells expressing AQP1 or GFP. Scale bars, 10 μm.

    Journal: Nature Communications

    Article Title: Non-invasive imaging using reporter genes altering cellular water permeability

    doi: 10.1038/ncomms13891

    Figure Lengend Snippet: AQP1 functions as a genetically encoded reporter for diffusion-weighted MRI. ( a ) Illustration of the impact of aquaporin expression on water diffusion across the cell membrane and the resulting decrease in diffusion-weighted signal intensity. ( b ) Diffusion-weighted images of CHO, U87 and Neuro 2a cell pellets expressing AQP1 or GFP, acquired using a b -value of ∼1,000 s mm −2 . Scale bars, 3 mm. ( c ) ADC of water in CHO, U87 and Neuro 2a cells expressing AQP1 relative to GFP controls, measured at Δ eff =398 ms. Transgene expression in CHO cells was induced with 1 μg ml −1 doxycycline, whereas U87 and Neuro 2a cells express AQP1 from a constitutive promoter. n =4 (U87, Neuro 2a) and 5 (CHO) biological replicates. ( d ) Longitudinal ( T 1 ) and ( e ) transverse ( T 2 ) relaxation rates in cells expressing AQP1 or GFP. n =3 (Neuro 2a, CHO) or 4 (U87) biological replicates. ( f ) Cell viability on AQP1 or GFP expression. n =12 (resazurin assay), 6 (ATP content), 4 (LDH release) and 3 (ethidium staining) biological replicates. Error bars±s.e.m. ( g ) Phase-contrast images of CHO, U87 and Neuro 2a cells expressing AQP1 or GFP. Scale bars, 10 μm.

    Article Snippet: Determination of cell viability Cell viability was determined using four different approaches including staining with ethidium homodimer-1 (Thermo Fisher) and measurement of resazurin reduction (CellTiter-Blue assay, Promega), ATP content (CellTiter-Glo assay, Promega) and lactate dehydrogenase release (CytoOne, Promega).

    Techniques: Diffusion-based Assay, Magnetic Resonance Imaging, Expressing, Mass Spectrometry, Resazurin Assay, Staining