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Promega atp celltitre glo reagent
Atp Celltitre Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp celltitre glo reagent/product/Promega
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
atp celltitre glo reagent - by Bioz Stars, 2020-04
91/100 stars

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Article Title: Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells
Article Snippet: ATP levels Intracellular ATP levels were measured using the ATP CellTitre Glo reagent (Promega, Madison, USA).

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    Promega atp reagent
    Application of 3D culture with LA717 to anti-cancer drug evaluation. (a) A549 cells were grown at 1000 cells/well in 96-well cell-attachable plates in DMEM (2D) or in low-attachment plates with 0.030% (w/v) LA717 (3D). After 1 day of culture, anti-cancer drugs or DMSO (Control) were added. The cells were further cultured for 7 days and the cell number was counted using <t>ATP</t> assay. Data represent means ± SD of 3 independent experiments. Statistical significance was analysed with Tukey’s test. (b) Anti-cancer drug-treated cells were prepared as in ( a ). The Y-axis indicates the Caspase <t>3/7-Glo</t> value (apoptosis activity) normalised to the ATP value (cell number). Statistical significance was analysed with Dunnett’s test (vs. Control). Data represent means ± SD of 3 independent experiments.
    Atp Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp reagent/product/Promega
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    atp reagent - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    Promega celltiter glo 3d reagent
    Mini-ring approach to unveil drug response patterns in PDTOs. a Morphology of all PDTOs established in this study as visualized by bright-field microscopy. Morphology and 3D organization of the samples is highly variable. For instance, some of Patient #3 cells are arranged in fascicles within the Matrigel, likely representing the sarcomatous component of the tumor. Scale bar, 100 µm. b Results of kinase screening experiment for Patient #1 PDTOs. Three readouts were used for this assay: ATP quantification as measured by <t>CellTiter-Glo</t> 3D and organoid number or size quantification evaluated by bright-field imaging. Bright-field images were segmented and quantified using the Celigo S Imaging Cell Cytometer Software. Both organoid number and total area were evaluated for their ability to capture response to drugs. In this plot, each vertical line is one drug, all 240 tested are shown. Values are normalized to the respective vehicle controls for each method and expressed as %. Average Z-score calculated as reported in Methods. c A representative image of the effects of the indicated drug treatments as visualized by the Celigo cell imager. Scale bar, 100 µm. d Small-scale kinase assay on Patient #1 primary PDTOs and PDX-derived cells. ATP readout. Four molecules not present in the primary screening were tested. Flavopiridol and BS-181 HCl are included as positive and negative control, respectively. t -test, ** p
    Celltiter Glo 3d Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo 3d reagent/product/Promega
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    celltiter glo 3d reagent - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Application of 3D culture with LA717 to anti-cancer drug evaluation. (a) A549 cells were grown at 1000 cells/well in 96-well cell-attachable plates in DMEM (2D) or in low-attachment plates with 0.030% (w/v) LA717 (3D). After 1 day of culture, anti-cancer drugs or DMSO (Control) were added. The cells were further cultured for 7 days and the cell number was counted using ATP assay. Data represent means ± SD of 3 independent experiments. Statistical significance was analysed with Tukey’s test. (b) Anti-cancer drug-treated cells were prepared as in ( a ). The Y-axis indicates the Caspase 3/7-Glo value (apoptosis activity) normalised to the ATP value (cell number). Statistical significance was analysed with Dunnett’s test (vs. Control). Data represent means ± SD of 3 independent experiments.

    Journal: Scientific Reports

    Article Title: Novel 3D Liquid Cell Culture Method for Anchorage-independent Cell Growth, Cell Imaging and Automated Drug Screening

    doi: 10.1038/s41598-018-21950-5

    Figure Lengend Snippet: Application of 3D culture with LA717 to anti-cancer drug evaluation. (a) A549 cells were grown at 1000 cells/well in 96-well cell-attachable plates in DMEM (2D) or in low-attachment plates with 0.030% (w/v) LA717 (3D). After 1 day of culture, anti-cancer drugs or DMSO (Control) were added. The cells were further cultured for 7 days and the cell number was counted using ATP assay. Data represent means ± SD of 3 independent experiments. Statistical significance was analysed with Tukey’s test. (b) Anti-cancer drug-treated cells were prepared as in ( a ). The Y-axis indicates the Caspase 3/7-Glo value (apoptosis activity) normalised to the ATP value (cell number). Statistical significance was analysed with Dunnett’s test (vs. Control). Data represent means ± SD of 3 independent experiments.

    Article Snippet: For the ATP assay, an equal volume of ATP reagent (CellTiter-Glo® Luminescent Cell Viability Assay; Promega, Wisconsin, USA) was added to the culture medium and the luminescence intensity (Relative Light Unit (RLU) value) was measured using FlexStation3 (Molecular Devices, California, USA).

    Techniques: Cell Culture, ATP Assay, Activity Assay

    Cells cultured with gellan gum ( FP 001) are sensitive to epidermal growth factor ( EGF ) family stimulation. (a) SKOV 3 cells were inoculated at a density of 300 cells/well into 96‐well normal plates (2‐D) and at a density of 5000 cells/well into 96‐well low attachment plates (3‐D) with or without 0.015% (w/v) FP 001. After 1 day of culture, growth factors and medium (Control) were added to each cell culture. The cells were subsequently cultured for 4 days and the numbers of cultured cells were counted using the ATP assay. (b) SKOV 3 cells were inoculated as in (a). After 1 day of culture, 100 ng/ mL heparin‐binding EGF ‐like growth factor ( HB ‐ EGF ) or medium (Vehicle), and anticancer drugs and DMSO (Control) were added to each cell culture. The cells were subsequently cultured for 3 days and the numbers of cultured cells were counted. (c) SKOV 3 cells were treated with anticancer drugs for 5 days as described for (b). The numbers of cultured cells were counted using the ATP assay and apoptosis activity was evaluated with the Caspase 3/7‐Glo assay. Statistical significance was analyzed with Dunnett's test ( vs . Control). Data represent means ± SD for three independent experiments.

    Journal: Cancer Science

    Article Title: Novel 3‐D cell culture system for in vitro evaluation of anticancer drugs under anchorage‐independent conditions

    doi: 10.1111/cas.13095

    Figure Lengend Snippet: Cells cultured with gellan gum ( FP 001) are sensitive to epidermal growth factor ( EGF ) family stimulation. (a) SKOV 3 cells were inoculated at a density of 300 cells/well into 96‐well normal plates (2‐D) and at a density of 5000 cells/well into 96‐well low attachment plates (3‐D) with or without 0.015% (w/v) FP 001. After 1 day of culture, growth factors and medium (Control) were added to each cell culture. The cells were subsequently cultured for 4 days and the numbers of cultured cells were counted using the ATP assay. (b) SKOV 3 cells were inoculated as in (a). After 1 day of culture, 100 ng/ mL heparin‐binding EGF ‐like growth factor ( HB ‐ EGF ) or medium (Vehicle), and anticancer drugs and DMSO (Control) were added to each cell culture. The cells were subsequently cultured for 3 days and the numbers of cultured cells were counted. (c) SKOV 3 cells were treated with anticancer drugs for 5 days as described for (b). The numbers of cultured cells were counted using the ATP assay and apoptosis activity was evaluated with the Caspase 3/7‐Glo assay. Statistical significance was analyzed with Dunnett's test ( vs . Control). Data represent means ± SD for three independent experiments.

    Article Snippet: For the ATP assay, an equal volume of the ATP reagent (CellTiter‐Glo Luminescent Cell Viability Assay; Promega, Madison, WI, USA) was added to the cell culture, and the luminescence intensity (relative light unit value) was measured using FlexStation3 (Molecular Devices).

    Techniques: Cell Culture, ATP Assay, Binding Assay, Activity Assay, Glo Assay

    Cells grown under 3‐D conditions with gellan gum ( FP 001) are more sensitive to molecularly targeted anticancer agents. (a) A549 cells were inoculated at a density of 2000 cells/well into 96‐well normal plates in 10% FBS ‐containing DMEM (2‐D) and into 96‐well low attachment plates in 10% FBS ‐containing DMEM (3‐D) with or without 0.015% (w/v) gellan gum ( FP 001). After 1 day of culture, anticancer drugs and DMSO (Control) were added to each cell culture. Cells were subsequently cultured for 8 days and the cell numbers were counted. (b) Anticancer drug‐treated cells were prepared as in (a). The Y ‐axis indicates the Caspase 3/7‐Glo value (apoptosis activity) normalized to the ATP value (cell number). Statistical significance was analyzed with Dunnett's test ( vs . Control). (c) A549 cells were inoculated as in (a). Paclitaxel, MK 2206, and DMSO (Control) were added to each cell culture for 72 h and the populations of annexin V + /propidium iodide ( PI ) − cells were measured. Statistical significance was analyzed with Student's t ‐test. Data represent means ± SD for three independent experiments. n.s., not significant.

    Journal: Cancer Science

    Article Title: Novel 3‐D cell culture system for in vitro evaluation of anticancer drugs under anchorage‐independent conditions

    doi: 10.1111/cas.13095

    Figure Lengend Snippet: Cells grown under 3‐D conditions with gellan gum ( FP 001) are more sensitive to molecularly targeted anticancer agents. (a) A549 cells were inoculated at a density of 2000 cells/well into 96‐well normal plates in 10% FBS ‐containing DMEM (2‐D) and into 96‐well low attachment plates in 10% FBS ‐containing DMEM (3‐D) with or without 0.015% (w/v) gellan gum ( FP 001). After 1 day of culture, anticancer drugs and DMSO (Control) were added to each cell culture. Cells were subsequently cultured for 8 days and the cell numbers were counted. (b) Anticancer drug‐treated cells were prepared as in (a). The Y ‐axis indicates the Caspase 3/7‐Glo value (apoptosis activity) normalized to the ATP value (cell number). Statistical significance was analyzed with Dunnett's test ( vs . Control). (c) A549 cells were inoculated as in (a). Paclitaxel, MK 2206, and DMSO (Control) were added to each cell culture for 72 h and the populations of annexin V + /propidium iodide ( PI ) − cells were measured. Statistical significance was analyzed with Student's t ‐test. Data represent means ± SD for three independent experiments. n.s., not significant.

    Article Snippet: For the ATP assay, an equal volume of the ATP reagent (CellTiter‐Glo Luminescent Cell Viability Assay; Promega, Madison, WI, USA) was added to the cell culture, and the luminescence intensity (relative light unit value) was measured using FlexStation3 (Molecular Devices).

    Techniques: Cell Culture, Activity Assay

    Application of 3D culture with LA717 to anti-cancer drug evaluation. (a) A549 cells were grown at 1000 cells/well in 96-well cell-attachable plates in DMEM (2D) or in low-attachment plates with 0.030% (w/v) LA717 (3D). After 1 day of culture, anti-cancer drugs or DMSO (Control) were added. The cells were further cultured for 7 days and the cell number was counted using ATP assay. Data represent means ± SD of 3 independent experiments. Statistical significance was analysed with Tukey’s test. (b) Anti-cancer drug-treated cells were prepared as in ( a ). The Y-axis indicates the Caspase 3/7-Glo value (apoptosis activity) normalised to the ATP value (cell number). Statistical significance was analysed with Dunnett’s test (vs. Control). Data represent means ± SD of 3 independent experiments.

    Journal: Scientific Reports

    Article Title: Novel 3D Liquid Cell Culture Method for Anchorage-independent Cell Growth, Cell Imaging and Automated Drug Screening

    doi: 10.1038/s41598-018-21950-5

    Figure Lengend Snippet: Application of 3D culture with LA717 to anti-cancer drug evaluation. (a) A549 cells were grown at 1000 cells/well in 96-well cell-attachable plates in DMEM (2D) or in low-attachment plates with 0.030% (w/v) LA717 (3D). After 1 day of culture, anti-cancer drugs or DMSO (Control) were added. The cells were further cultured for 7 days and the cell number was counted using ATP assay. Data represent means ± SD of 3 independent experiments. Statistical significance was analysed with Tukey’s test. (b) Anti-cancer drug-treated cells were prepared as in ( a ). The Y-axis indicates the Caspase 3/7-Glo value (apoptosis activity) normalised to the ATP value (cell number). Statistical significance was analysed with Dunnett’s test (vs. Control). Data represent means ± SD of 3 independent experiments.

    Article Snippet: For the ATP assay, an equal volume of ATP reagent (CellTiter-Glo® Luminescent Cell Viability Assay; Promega, Wisconsin, USA) was added to the culture medium and the luminescence intensity (Relative Light Unit (RLU) value) was measured using FlexStation3 (Molecular Devices, California, USA).

    Techniques: Cell Culture, ATP Assay, Activity Assay

    Mini-ring approach to unveil drug response patterns in PDTOs. a Morphology of all PDTOs established in this study as visualized by bright-field microscopy. Morphology and 3D organization of the samples is highly variable. For instance, some of Patient #3 cells are arranged in fascicles within the Matrigel, likely representing the sarcomatous component of the tumor. Scale bar, 100 µm. b Results of kinase screening experiment for Patient #1 PDTOs. Three readouts were used for this assay: ATP quantification as measured by CellTiter-Glo 3D and organoid number or size quantification evaluated by bright-field imaging. Bright-field images were segmented and quantified using the Celigo S Imaging Cell Cytometer Software. Both organoid number and total area were evaluated for their ability to capture response to drugs. In this plot, each vertical line is one drug, all 240 tested are shown. Values are normalized to the respective vehicle controls for each method and expressed as %. Average Z-score calculated as reported in Methods. c A representative image of the effects of the indicated drug treatments as visualized by the Celigo cell imager. Scale bar, 100 µm. d Small-scale kinase assay on Patient #1 primary PDTOs and PDX-derived cells. ATP readout. Four molecules not present in the primary screening were tested. Flavopiridol and BS-181 HCl are included as positive and negative control, respectively. t -test, ** p

    Journal: Communications Biology

    Article Title: A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids

    doi: 10.1038/s42003-019-0305-x

    Figure Lengend Snippet: Mini-ring approach to unveil drug response patterns in PDTOs. a Morphology of all PDTOs established in this study as visualized by bright-field microscopy. Morphology and 3D organization of the samples is highly variable. For instance, some of Patient #3 cells are arranged in fascicles within the Matrigel, likely representing the sarcomatous component of the tumor. Scale bar, 100 µm. b Results of kinase screening experiment for Patient #1 PDTOs. Three readouts were used for this assay: ATP quantification as measured by CellTiter-Glo 3D and organoid number or size quantification evaluated by bright-field imaging. Bright-field images were segmented and quantified using the Celigo S Imaging Cell Cytometer Software. Both organoid number and total area were evaluated for their ability to capture response to drugs. In this plot, each vertical line is one drug, all 240 tested are shown. Values are normalized to the respective vehicle controls for each method and expressed as %. Average Z-score calculated as reported in Methods. c A representative image of the effects of the indicated drug treatments as visualized by the Celigo cell imager. Scale bar, 100 µm. d Small-scale kinase assay on Patient #1 primary PDTOs and PDX-derived cells. ATP readout. Four molecules not present in the primary screening were tested. Flavopiridol and BS-181 HCl are included as positive and negative control, respectively. t -test, ** p

    Article Snippet: ATP assay After the organoid release with dispase as indicated above, 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) is added to each well followed by 1 min of vigorous shaking.

    Techniques: Microscopy, Imaging, Cytometry, Software, Kinase Assay, Derivative Assay, Negative Control