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Promega atp assay cell titer glo
Atp Assay Cell Titer Glo, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp assay cell titer glo/product/Promega
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
atp assay cell titer glo - by Bioz Stars, 2020-03
79/100 stars

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ATP Assay:

Article Title: Competitive HIF Prolyl Hydroxylase Inhibitors Show Protection against Oxidative Stress by a Mechanism Partially Dependent on Glycolysis
Article Snippet: .. ATP Assay Cell-titer Glo (G7570, Promega) was used to determine the total cellular ATP levels. .. This assay is based on the ATP-catalyzed monooxygenation of luciferin to luciferase.

Luciferase:

Article Title: Competitive HIF Prolyl Hydroxylase Inhibitors Show Protection against Oxidative Stress by a Mechanism Partially Dependent on Glycolysis
Article Snippet: ATP Assay Cell-titer Glo (G7570, Promega) was used to determine the total cellular ATP levels. .. This assay is based on the ATP-catalyzed monooxygenation of luciferin to luciferase.

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  • 90
    Promega cell titer glo luminescent cell viability assay kit
    IAV-induced cell death involves both apoptosis and necroptosis. Differentiated THP1 cells were either left untreated or treated for 1 h with the caspase inhibitor QVD (20 μM) and one of the following pharmacological inhibitors: Nec-1 (10 μM) for RIPK1 (A), GSK872 (5 μM) for RIPK3 (B), and NSA (1 μM) for MLKL (C). Following chemical treatment, the cells were either mock infected or infected with PR8 virus at an MOI of 5. At 18 to 20 h p.i., cell death was assessed by measuring the <t>ATP</t> concentration in cells using a Cell <t>Titer-Glo</t> luminescent viability assay.
    Cell Titer Glo Luminescent Cell Viability Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell titer glo luminescent cell viability assay kit/product/Promega
    Average 90 stars, based on 174 article reviews
    Price from $9.99 to $1999.99
    cell titer glo luminescent cell viability assay kit - by Bioz Stars, 2020-03
    90/100 stars
      Buy from Supplier

    99
    Promega cell titer glo reagent
    Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . <t>ATP</t> release into the media was measured using cell-titer <t>glo</t> assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p
    Cell Titer Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell titer glo reagent/product/Promega
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    cell titer glo reagent - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Promega cell titer glo assay
    Caspase-8 activity rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using <t>Promega’s</t> <t>Caspase-Glo</t> assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; * P ≤ 0.05, *** P ≤ 0.001)
    Cell Titer Glo Assay, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell titer glo assay/product/Promega
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    cell titer glo assay - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    75
    Promega atp based cell titer glo luminescent cell viability kit
    Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction is protective against LPS-mediated neuronal injury in culture. a Oxidative phosphorylation and glycolytic rate was measured with Seahorse Extracellular Flux in primary neurons were treated with 0.1 mg/ml LPS in the presence/absence of P110 (1 mM) for 24 h ( n = 6). b Cellular <t>ATP</t> level was measured using <t>CellTiter-Glo®</t> after treatment as in a and represented as fold change of control. c Mitochondrial membrane potential was measured using TMRE after treatment as in a and represented as fold change of control ( n = 6). d Drp1 levels and p53 levels were quantified by immunoblotting in enriched mitochondrial fractions and represented as fold change of control. VDAC was used as a loading control ( n = 3). e LDH release was measured after treatment as in a and represented as % of Control (Con) ( n = 6). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated
    Atp Based Cell Titer Glo Luminescent Cell Viability Kit, supplied by Promega, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp based cell titer glo luminescent cell viability kit/product/Promega
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp based cell titer glo luminescent cell viability kit - by Bioz Stars, 2020-03
    75/100 stars
      Buy from Supplier

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    IAV-induced cell death involves both apoptosis and necroptosis. Differentiated THP1 cells were either left untreated or treated for 1 h with the caspase inhibitor QVD (20 μM) and one of the following pharmacological inhibitors: Nec-1 (10 μM) for RIPK1 (A), GSK872 (5 μM) for RIPK3 (B), and NSA (1 μM) for MLKL (C). Following chemical treatment, the cells were either mock infected or infected with PR8 virus at an MOI of 5. At 18 to 20 h p.i., cell death was assessed by measuring the ATP concentration in cells using a Cell Titer-Glo luminescent viability assay.

    Journal: Journal of Virology

    Article Title: The NS1 Protein of Influenza A Virus Participates in Necroptosis by Interacting with MLKL and Increasing Its Oligomerization and Membrane Translocation

    doi: 10.1128/JVI.01835-18

    Figure Lengend Snippet: IAV-induced cell death involves both apoptosis and necroptosis. Differentiated THP1 cells were either left untreated or treated for 1 h with the caspase inhibitor QVD (20 μM) and one of the following pharmacological inhibitors: Nec-1 (10 μM) for RIPK1 (A), GSK872 (5 μM) for RIPK3 (B), and NSA (1 μM) for MLKL (C). Following chemical treatment, the cells were either mock infected or infected with PR8 virus at an MOI of 5. At 18 to 20 h p.i., cell death was assessed by measuring the ATP concentration in cells using a Cell Titer-Glo luminescent viability assay.

    Article Snippet: At 18 to 20 h postinfection (p.i.), the ATP concentration in cells was measured using a Cell Titer-Glo luminescent cell viability assay kit according to the manufacturer’s instructions (Promega, Madison, WI).

    Techniques: Infection, Concentration Assay, Viability Assay

    Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . ATP release into the media was measured using cell-titer glo assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p

    Journal: Molecular Metabolism

    Article Title: Pannexin 1 is required for full activation of insulin-stimulated glucose uptake in adipocytes

    doi: 10.1016/j.molmet.2015.06.009

    Figure Lengend Snippet: Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . ATP release into the media was measured using cell-titer glo assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p

    Article Snippet: ATP concentrations in supernatants were measured using Promega Cell Titer Glo reagent.

    Techniques: Staining, Glo Assay

    Caspase-8 activity rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using Promega’s Caspase-Glo assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; * P ≤ 0.05, *** P ≤ 0.001)

    Journal: Cancer drug resistance (Alhambra, Calif.)

    Article Title: Improved potency of F10 relative to 5-fluorouracil in colorectal cancer cells with p53 mutations

    doi: 10.20517/cdr.2018.01

    Figure Lengend Snippet: Caspase-8 activity rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using Promega’s Caspase-Glo assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; * P ≤ 0.05, *** P ≤ 0.001)

    Article Snippet: F10 is highly potent regardless of TP53 status We assessed growth inhibition in the four isogenic cell lines by quantifying changes in cell viability after 72 h of drug treatment using a Cell-Titer Glo assay (Promega) which quantifies cellular ATP levels.

    Techniques: Activity Assay, Caspase-Glo Assay

    Cell viability rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Cell viability was determined using Promega’s CellTiter-Glo ATP assay after 48 h and calculated following blank subtraction relative to respective controls. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; * P ≤ 0.05, **** P ≤ 0.0001)

    Journal: Cancer drug resistance (Alhambra, Calif.)

    Article Title: Improved potency of F10 relative to 5-fluorouracil in colorectal cancer cells with p53 mutations

    doi: 10.20517/cdr.2018.01

    Figure Lengend Snippet: Cell viability rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Cell viability was determined using Promega’s CellTiter-Glo ATP assay after 48 h and calculated following blank subtraction relative to respective controls. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; * P ≤ 0.05, **** P ≤ 0.0001)

    Article Snippet: F10 is highly potent regardless of TP53 status We assessed growth inhibition in the four isogenic cell lines by quantifying changes in cell viability after 72 h of drug treatment using a Cell-Titer Glo assay (Promega) which quantifies cellular ATP levels.

    Techniques: ATP Assay

    Caspase-3/7 activity rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using Promega’s Caspase-Glo assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; **** P ≤ 0.0001)

    Journal: Cancer drug resistance (Alhambra, Calif.)

    Article Title: Improved potency of F10 relative to 5-fluorouracil in colorectal cancer cells with p53 mutations

    doi: 10.20517/cdr.2018.01

    Figure Lengend Snippet: Caspase-3/7 activity rescue data. HCT116 p53 +/+ and HCT116 p53 −/− cells were co-treated with indicated concentrations of (left) F10 or (right) 5-FU along with indicated nucleoside (uridine = 1 mmol/L, thymidine = 80 μmol/L) for 48 h. Caspase activity per cell viability was determined using Promega’s Caspase-Glo assay after 48 h and calculated following blank subtraction relative to respective control activity. Experiments done in triplicate ± SEM. P -values calculated using GraphPad Prism ( n = 4; **** P ≤ 0.0001)

    Article Snippet: F10 is highly potent regardless of TP53 status We assessed growth inhibition in the four isogenic cell lines by quantifying changes in cell viability after 72 h of drug treatment using a Cell-Titer Glo assay (Promega) which quantifies cellular ATP levels.

    Techniques: Activity Assay, Caspase-Glo Assay

    Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction is protective against LPS-mediated neuronal injury in culture. a Oxidative phosphorylation and glycolytic rate was measured with Seahorse Extracellular Flux in primary neurons were treated with 0.1 mg/ml LPS in the presence/absence of P110 (1 mM) for 24 h ( n = 6). b Cellular ATP level was measured using CellTiter-Glo® after treatment as in a and represented as fold change of control. c Mitochondrial membrane potential was measured using TMRE after treatment as in a and represented as fold change of control ( n = 6). d Drp1 levels and p53 levels were quantified by immunoblotting in enriched mitochondrial fractions and represented as fold change of control. VDAC was used as a loading control ( n = 3). e LDH release was measured after treatment as in a and represented as % of Control (Con) ( n = 6). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated

    Journal: Journal of Neuroinflammation

    Article Title: Mitochondrial dysfunction mediated through dynamin-related protein 1 (Drp1) propagates impairment in blood brain barrier in septic encephalopathy

    doi: 10.1186/s12974-019-1689-8

    Figure Lengend Snippet: Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction is protective against LPS-mediated neuronal injury in culture. a Oxidative phosphorylation and glycolytic rate was measured with Seahorse Extracellular Flux in primary neurons were treated with 0.1 mg/ml LPS in the presence/absence of P110 (1 mM) for 24 h ( n = 6). b Cellular ATP level was measured using CellTiter-Glo® after treatment as in a and represented as fold change of control. c Mitochondrial membrane potential was measured using TMRE after treatment as in a and represented as fold change of control ( n = 6). d Drp1 levels and p53 levels were quantified by immunoblotting in enriched mitochondrial fractions and represented as fold change of control. VDAC was used as a loading control ( n = 3). e LDH release was measured after treatment as in a and represented as % of Control (Con) ( n = 6). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated

    Article Snippet: ATP measurements Relative intracellular ATP levels were determined using ATP-based Cell Titer-Glo Luminescent Cell Viability kit (Promega), which causes cell lysis and generates a luminescent signal proportional to the amount of ATP present.

    Techniques: Inhibition