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Promega atp assay cell titer glo reagent
Atp Assay Cell Titer Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/atp assay cell titer glo reagent/product/Promega
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
atp assay cell titer glo reagent - by Bioz Stars, 2020-04
91/100 stars

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ATP Assay:

Article Title: Cholesterol esterification inhibition and imatinib treatment synergistically inhibit growth of BCR-ABL mutation-independent resistant chronic myelogenous leukemia
Article Snippet: .. Cell viability after treatment for 72 hours was measured by intensity of luminescent signal as read by a SpectraMax M5 Plate Reader using the ATP assay Cell Titer Glo reagent from Promega. ..

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Article Title: Cholesterol esterification inhibition and imatinib treatment synergistically inhibit growth of BCR-ABL mutation-independent resistant chronic myelogenous leukemia
Article Snippet: Cell viability after treatment for 72 hours was measured by intensity of luminescent signal as read by a SpectraMax M5 Plate Reader using the ATP assay Cell Titer Glo reagent from Promega. .. Combination index was analyzed by the Chou-Talalay method using CompuSyn software.

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  • 98
    Promega cell titer glo reagent
    Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . <t>ATP</t> release into the media was measured using cell-titer <t>glo</t> assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p
    Cell Titer Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell titer glo reagent/product/Promega
    Average 98 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    cell titer glo reagent - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    93
    Promega cell titer glo luminescent assay reagent
    The effect of pitavastatin-zoledronic acid combinations on cell death. ( A ) Dead cells were measured by trypan blue staining after 72 and 96 hr of exposure to the indicated drug concentration. ( B ) Relative cell viability was measured by <t>celltiter-Glo</t> assay (ATP) after 72 hrs exposure to the indicated drug concentration. In both assays, the results (mean ± SD; n = 3) were compared to the effect expected for an additive interaction calculated using the Bliss independence criterion (solid line for each drug combination) and determined using the measured effect of the individual drugs in each individual experiment. Results were significantly different from the expected Bliss effect where shown ( *P
    Cell Titer Glo Luminescent Assay Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell titer glo luminescent assay reagent/product/Promega
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    cell titer glo luminescent assay reagent - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    99
    Promega celltiter glo 2 0 cell titer glo 2 0 reagent
    The anti-ferroptotic activity of vitamin E and its metabolites. (A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by <t>CellTiter-Glo</t> ® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E ( αT ), its metabolites ( αTQ , αTHQ ), or the synthetic carbochroman ( αTCC ) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPY TM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.
    Celltiter Glo 2 0 Cell Titer Glo 2 0 Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltiter glo 2 0 cell titer glo 2 0 reagent/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    celltiter glo 2 0 cell titer glo 2 0 reagent - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    91
    Promega atp assay cell titer glo reagent
    The anti-ferroptotic activity of vitamin E and its metabolites. (A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by <t>CellTiter-Glo</t> ® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E ( αT ), its metabolites ( αTQ , αTHQ ), or the synthetic carbochroman ( αTCC ) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPY TM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.
    Atp Assay Cell Titer Glo Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp assay cell titer glo reagent/product/Promega
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp assay cell titer glo reagent - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . ATP release into the media was measured using cell-titer glo assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p

    Journal: Molecular Metabolism

    Article Title: Pannexin 1 is required for full activation of insulin-stimulated glucose uptake in adipocytes

    doi: 10.1016/j.molmet.2015.06.009

    Figure Lengend Snippet: Pannexin 1 channel function in adipocytes is regulated by alpha-adrenergic stimulation or via caspase-mediated C-terminal cleavage during apoptosis . ( A ) 3T3-L1 adipocytes were treated with indicated concentrations of phenylephrine (PE) for 30 min and then stained with 1 μM YO-PRO ® and 1 μg/mL Hoechst for 10 min. Experiment was performed in triplicate. Total cells were quantitated by counting Hoechst-positive cells. Cells positive for YO-PRO ® indicate cells in which Panx1 channels have been activated and opened, allowing dye to enter. Data are expressed as mean ± s.e.m. *p = 0.0087 by Student's t-test. ( B ) 3T3-L1 adipocytes were treated with 5 μM PE with or without pretreatment with a Panx1 intracellular loop peptide (IL2) or a C-terminal peptide (CT2). The IL2 peptide corresponds to a region of the Panx1 intracellular loop (aa 191–200) while the CT2 peptide is from a region of the C-terminal tail corresponding to aa 381–390 (inset) [48] . ATP release into the media was measured using cell-titer glo assay (Promega). Experiment was performed in triplicate. Data are expressed as mean ± s.e.m. *p

    Article Snippet: ATP concentrations in supernatants were measured using Promega Cell Titer Glo reagent.

    Techniques: Staining, Glo Assay

    The effect of pitavastatin-zoledronic acid combinations on cell death. ( A ) Dead cells were measured by trypan blue staining after 72 and 96 hr of exposure to the indicated drug concentration. ( B ) Relative cell viability was measured by celltiter-Glo assay (ATP) after 72 hrs exposure to the indicated drug concentration. In both assays, the results (mean ± SD; n = 3) were compared to the effect expected for an additive interaction calculated using the Bliss independence criterion (solid line for each drug combination) and determined using the measured effect of the individual drugs in each individual experiment. Results were significantly different from the expected Bliss effect where shown ( *P

    Journal: Scientific Reports

    Article Title: Inhibition of the mevalonate pathway augments the activity of pitavastatin against ovarian cancer cells

    doi: 10.1038/s41598-017-08649-9

    Figure Lengend Snippet: The effect of pitavastatin-zoledronic acid combinations on cell death. ( A ) Dead cells were measured by trypan blue staining after 72 and 96 hr of exposure to the indicated drug concentration. ( B ) Relative cell viability was measured by celltiter-Glo assay (ATP) after 72 hrs exposure to the indicated drug concentration. In both assays, the results (mean ± SD; n = 3) were compared to the effect expected for an additive interaction calculated using the Bliss independence criterion (solid line for each drug combination) and determined using the measured effect of the individual drugs in each individual experiment. Results were significantly different from the expected Bliss effect where shown ( *P

    Article Snippet: Cell Titer-Glo Luminescent Assay (ATP-assay) Cell growth assays were prepared as described above but instead of staining with SRB, intracellular ATP level was quantitated using the cell Titer-Glo Luminescent assay reagent (Promega, Madison, WI, USA).

    Techniques: Staining, Concentration Assay, Glo Assay

    The anti-ferroptotic activity of vitamin E and its metabolites. (A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by CellTiter-Glo ® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E ( αT ), its metabolites ( αTQ , αTHQ ), or the synthetic carbochroman ( αTCC ) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPY TM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.

    Journal: PLoS ONE

    Article Title: Vitamin E hydroquinone is an endogenous regulator of ferroptosis via redox control of 15-lipoxygenase

    doi: 10.1371/journal.pone.0201369

    Figure Lengend Snippet: The anti-ferroptotic activity of vitamin E and its metabolites. (A) Ferroptosis was induced in Q7 cells by RSL3 treatment (2 μM 18 h), as measured by CellTiter-Glo ® 2.0 ATP assay. The dose-dependent cytoprotective activity of vitamin E ( αT ), its metabolites ( αTQ , αTHQ ), or the synthetic carbochroman ( αTCC ) was assessed by co-treatment (in 24-point titration) with RSL3. Curves are representative of ≥9 independent experiments, of which 7 evaluated αTCC to a top concentration of 30 μM. (B) Ferroptosis was initiated in BODIPY TM 581/591 C11-prelabeled Q7 cells by RSL3 (2 μM) treatment, with cellular lipid oxidation assessed by the rate of change in green cellular fluorescence using time-lapse microscopy. Rescue compounds were co-treated (in 6-point titration) with RSL3. Mean ± SEM shown, (n = 6 to 9 technical replicates for each condition. Results are representative of 2 independent experiments.

    Article Snippet: After a 15-minute equilibration to room temperature, cell number/viability was assessed using the CellTiter-Glo® 2.0 Cell Titer Glo 2.0 reagent (a luminescence-based assay to quantitate ATP; Promega) which was added using the Multidrop™ Combi Reagent Dispenser (Thermo Fisher Scientific).

    Techniques: Activity Assay, ATP Assay, Titration, Concentration Assay, Fluorescence, Time-lapse Microscopy