Journal: Advances in Pharmacological Sciences

Article Title: Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

doi: 10.1155/2015/418673

Figure Lengend Snippet: Effect of raw extract and fractions from E. tereticornis on insulin-resistant C2C12 and HepG2 cells. Glucose uptake in insulin-resistant C2C12 cells (a) and glucose production in insulin-resistant HepG2 cells (b). Cells were treated with different concentrations of the fractions or raw extract and glucose was measured in cultured supernatant after 4 h of treatment by the glucose oxidase technique. Eu, crude methanol extract; Ins (100 nM insulin); Control R, insulin-resistant C2C12 myotubes as controls; Ins 100 R, insulin-resistant C2C12 myotubes treated with 100 nM insulin; Metf, metformin 1 mM positive control. Bar values correspond to the arithmetic mean of glucose concentration for each treatment/control glucose concentration, n = 6 (C2C12) and n = 3 (HepG2). ∗ Ins 100 nM versus control. ∗ Treatment versus control R. p < 0.05, Student t -test. Error bars represent SEM.

Article Snippet: The C2C12 (ATCCCRL-1772) mouse muscle cells and HepG2 (ATCC HB-8065) human liver carcinoma cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Cell Culture, Positive Control, Concentration Assay

Journal: Advances in Pharmacological Sciences

Article Title: Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice

doi: 10.1155/2015/418673

Figure Lengend Snippet: Effect of the fraction 2-4-25 enriched in compound 1 on glucose uptake in C2C12 cells. (a) Thin-layer chromatography. Fraction F2-4 was separated again on a silica gel column. Subfraction 22 shows a large amount of triterpenes; pure compound 1 obtained from subfractions 25 and 26 after TLC. (b) 2-4-25 fraction rich in compound 1 increases glucose uptake. n = 3. ∗ p < 0.05 versus control, Student t -test. (c) Structure of compound 1 : 3 β -hydroxy-urs-11-en-28,13 β -olide.

Article Snippet: The C2C12 (ATCCCRL-1772) mouse muscle cells and HepG2 (ATCC HB-8065) human liver carcinoma cells were purchased from ATCC (Manassas, VA, USA).

Techniques: Thin Layer Chromatography

Journal: Heliyon

Article Title: Janus kinase inhibitors modify the fatty acid profile of extracellular vesicles and modulate the immune response

doi: 10.1016/j.heliyon.2024.e24710

Figure Lengend Snippet: Size of extracellular vesicles derived from Jurkat and U937 cells treated with jakinibs . Extracellular vesicles were isolated from Jurkat (A) and U937 (B) cells cultured in the presence of 200 nM baricitinib (BARI), 25 μM itacitinib (ITA), or 0.5 % DMSO (vehicle control) for 24 h. The absolute sizes of EVs were determined by dynamic light scattering. C . EVs were negatively stained and analyzed under TEM. Images captured by UltraScan ®1000XP camera. Particle diameters are shown; n = 1 independent experiment.

Article Snippet: Extracellular vesicles were isolated from sorted lymphocytes, sorted monocytes, as well as from lymphoblastoid Jurkat (ATCC TIB-152) and promonocytic U937 (ATCCCRL-1593) cell lines exposed to jakinibs as follows.

Techniques: Derivative Assay, Isolation, Cell Culture, Staining

Journal: Heliyon

Article Title: Janus kinase inhibitors modify the fatty acid profile of extracellular vesicles and modulate the immune response

doi: 10.1016/j.heliyon.2024.e24710

Figure Lengend Snippet: Peripheral blood leukocytes internalize extracellular vesicles derived from lymphoid and monocytic cell lines. Whole blood samples (187.500 cells approximately) from healthy volunteers were stained with CD45-PeCy7 and incubated with different concentrations of CFSE-labeled EVs released from Jurkat and U937 cells exposed to 0.5 % DMSO, 200 nM bariticinib o 25 μM itacitinib for 24 h. Leukocyte (CD45 + cells) were further discriminated by granularity (SSC–H) to calculate the percentage of CFSE-EV + -neutrophils, -lymphocytes, and -monocytes after quenching the CFSE fluorescence on the cell surface with trypan blue. A. Representative dot plots of DMSO-U-EVs and ITA-U-EVs uptake by neutrophils. Internalization of J-EVs (B, C) and U-EVs (D, E) by neutrophils, lymphocytes, and monocytes following exposure to baricitinib (B, D) or itacitinib (C, D). Wilcoxon text for paired groups; n = 3 independent experiments. J: Jurkat, U: U937. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Extracellular vesicles were isolated from sorted lymphocytes, sorted monocytes, as well as from lymphoblastoid Jurkat (ATCC TIB-152) and promonocytic U937 (ATCCCRL-1593) cell lines exposed to jakinibs as follows.

Techniques: Derivative Assay, Staining, Incubation, Labeling, Fluorescence

Journal: Heliyon

Article Title: Janus kinase inhibitors modify the fatty acid profile of extracellular vesicles and modulate the immune response

doi: 10.1016/j.heliyon.2024.e24710

Figure Lengend Snippet: Effect of extracellular vesicles derived from lymphocytes and monocytes treated with jakinibs on platelet aggregation . Platelet rich plasma samples were exposed to A . J-EVs, B. L-EVs, C. M-EVs and D. U-EVs released in the presence of 0.5 % DMSO, 200 nM bariticinib, and 25 μM itacitinib (100 cells: 1250 EVs). After 8 m, percentages of light transmission were measured. Lines represent median ± IQR. *p < 0.05; **p < 0.01; ***p < 0.001; Kruskal Wallis and post-hoc Dunn's tests; n = 5 independent experiments. J: Jurkat, L: Lymphocytes, M: Monocytes, U: U937.

Article Snippet: Extracellular vesicles were isolated from sorted lymphocytes, sorted monocytes, as well as from lymphoblastoid Jurkat (ATCC TIB-152) and promonocytic U937 (ATCCCRL-1593) cell lines exposed to jakinibs as follows.

Techniques: Derivative Assay, Transmission Assay