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qRT-PCR analysis of ferroptosis-related gene expression after sevoflurane and H 2 O 2 exposure. Relative expression of GPX4, SLC7A11, ACSL4, and PTGS2 in Control, Sevo (3.3% for 6 h), H 2 O 2 (200 μM for 24 h), and Sevo + H 2 O 2 groups in C6 glioma cells and HA astrocytes. *P < 0.05 versus Control; significant comparisons are displayed with a single asterisk. Data represent mean ± SEM from three independent experiments. Two-way ANOVA (sevoflurane × H 2 O 2 ) was followed by Dunnett’s multiple-comparisons test against Control.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Integrated re-analysis of bulk and single-cell transcriptomic data reveals limited ferroptosis and neuroinflammation signatures after sevoflurane exposure in the aged rat hippocampus and human prefrontal cortex cells

doi: 10.3389/fcell.2026.1938601

Figure Lengend Snippet: qRT-PCR analysis of ferroptosis-related gene expression after sevoflurane and H 2 O 2 exposure. Relative expression of GPX4, SLC7A11, ACSL4, and PTGS2 in Control, Sevo (3.3% for 6 h), H 2 O 2 (200 μM for 24 h), and Sevo + H 2 O 2 groups in C6 glioma cells and HA astrocytes. *P < 0.05 versus Control; significant comparisons are displayed with a single asterisk. Data represent mean ± SEM from three independent experiments. Two-way ANOVA (sevoflurane × H 2 O 2 ) was followed by Dunnett’s multiple-comparisons test against Control.

Article Snippet: To examine regulation of key ferroptosis-related genes identified in the transcriptomic analyses, quantitative RT-PCR was performed in C6 rat glioma cells (ATCC CRL-2309) and HA human astrocytes (ATCC CRL-3050).

Techniques: Quantitative RT-PCR, Analysis, Gene Expression, Expressing, Control