mircut kit tiangen biotech china catalog number 4992860  (tiangen biotech co)

 
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    tiangen biotech co mircut kit tiangen biotech china catalog number 4992860
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    rt qpcr kit q511 02 vazyme biotech nanjing  (Vazyme Biotech Co)


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    Bioz Manufacturer Symbol Vazyme Biotech Co manufactures this product  
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    Vazyme Biotech Co rt qpcr kit q511 02 vazyme biotech nanjing
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    rt qpcr kit q511 02 vazyme biotech nanjing  (Vazyme Biotech Co)


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    Vazyme Biotech Co rt qpcr kit q511 02 vazyme biotech nanjing
    Effects of LNCs@CSF1R siRNA injection on tumor growth and immune environment in a colon cancer mouse model. Note ( A ) Visualization of colonic tumors in AOM and DSS-induced colon cancer mice; ( B ) Quantification of colonic tumor numbers in each group of mice; ( C ) H&E staining of tumor tissues; ( D ) Immunohistochemistry observation of the number of Ki67-positive cells; ( E ) Immunofluorescence observation of CD11b + Gr-1 + MDSCs (scale bar = 100 μm); ( F - G ) Flow cytometry analysis of effector cytokines IFNγ and GzmB produced by CD8 + T cells in tumor tissues after injection of LNCs@CSF1R siRNA; ( H - I ) <t>RT-qPCR</t> and ELISA analysis of mRNA and protein levels of iNOS, Arg1, CXCR2, TGF-β, and IL-10 in MDSC cells after injection of LNCs@CSF1R siRNA. * represents P < 0.05, ** represents P < 0.01. Each group consisted of 6 mice
    Rt Qpcr Kit Q511 02 Vazyme Biotech Nanjing, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Targeting CSF1R in myeloid-derived suppressor cells: insights into its immunomodulatory functions in colorectal cancer and therapeutic implications"

    Article Title: Targeting CSF1R in myeloid-derived suppressor cells: insights into its immunomodulatory functions in colorectal cancer and therapeutic implications

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-024-02584-4

    Effects of LNCs@CSF1R siRNA injection on tumor growth and immune environment in a colon cancer mouse model. Note ( A ) Visualization of colonic tumors in AOM and DSS-induced colon cancer mice; ( B ) Quantification of colonic tumor numbers in each group of mice; ( C ) H&E staining of tumor tissues; ( D ) Immunohistochemistry observation of the number of Ki67-positive cells; ( E ) Immunofluorescence observation of CD11b + Gr-1 + MDSCs (scale bar = 100 μm); ( F - G ) Flow cytometry analysis of effector cytokines IFNγ and GzmB produced by CD8 + T cells in tumor tissues after injection of LNCs@CSF1R siRNA; ( H - I ) RT-qPCR and ELISA analysis of mRNA and protein levels of iNOS, Arg1, CXCR2, TGF-β, and IL-10 in MDSC cells after injection of LNCs@CSF1R siRNA. * represents P < 0.05, ** represents P < 0.01. Each group consisted of 6 mice
    Figure Legend Snippet: Effects of LNCs@CSF1R siRNA injection on tumor growth and immune environment in a colon cancer mouse model. Note ( A ) Visualization of colonic tumors in AOM and DSS-induced colon cancer mice; ( B ) Quantification of colonic tumor numbers in each group of mice; ( C ) H&E staining of tumor tissues; ( D ) Immunohistochemistry observation of the number of Ki67-positive cells; ( E ) Immunofluorescence observation of CD11b + Gr-1 + MDSCs (scale bar = 100 μm); ( F - G ) Flow cytometry analysis of effector cytokines IFNγ and GzmB produced by CD8 + T cells in tumor tissues after injection of LNCs@CSF1R siRNA; ( H - I ) RT-qPCR and ELISA analysis of mRNA and protein levels of iNOS, Arg1, CXCR2, TGF-β, and IL-10 in MDSC cells after injection of LNCs@CSF1R siRNA. * represents P < 0.05, ** represents P < 0.01. Each group consisted of 6 mice

    Techniques Used: Injection, Staining, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Produced, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay


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    cs2 0106 5ml dead cell removal kit miltenyi biotech  (Miltenyi Biotec)

     
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    Miltenyi Biotec cs2 0106 5ml dead cell removal kit miltenyi biotech
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    assays lineage cell depletion kit miltenyi biotech 130 090 858 rneasy plus micro kit qiagen 74034 iscript cdna synthesis kit biorad 1708891  (Miltenyi Biotec)

     
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    Miltenyi Biotec assays lineage cell depletion kit miltenyi biotech 130 090 858 rneasy plus micro kit qiagen 74034 iscript cdna synthesis kit biorad 1708891
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