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Agilent technologies assay conditions hplc separation
<t>HPLC</t> chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the <t>Agilent</t> 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).
Assay Conditions Hplc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography

Journal: Molecular Vision

doi:

HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).
Figure Legend Snippet: HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Injection

HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).
Figure Legend Snippet: HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Injection

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Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography
Article Snippet: .. Assay conditions HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. .. A COSMOSIL 5C18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm, Nacalai Tesque, Inc., Kyoto Japan) was used and operated at 25 °C.

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    Agilent technologies assay conditions hplc separation
    <t>HPLC</t> chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the <t>Agilent</t> 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).
    Assay Conditions Hplc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/assay conditions hplc separation/product/Agilent technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    assay conditions hplc separation - by Bioz Stars, 2020-08
    85/100 stars
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    HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).

    Journal: Molecular Vision

    Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography

    doi:

    Figure Lengend Snippet: HPLC chromatograms of plasma mangiferin. HPLC separation was performed on plasma samples using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm,5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60 v:v). Typical chromatograms of blank plasma, blank plasma spiked with mangiferin and I.S., and rat plasma sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the plasma after the protein precipitation step. A : Typical chromatogram of blank plasma. B . Typical chromatogram of blank plasma spiked with standard mangiferin (5 μg/ml) and I.S. panel. C . Typical chromatogram of blood sample containing mangiferin (24.14 µg/ml) collected at 0.5 h after mangiferin administration (10 mg/kg, i.v.). D : Typical chromatogram of blood sample containing mangiferin (73.88 µg/ml) collected at 0.5 h after mangiferin administration (25 mg/kg, i.v.). E : Typical chromatogram of blood sample containing mangiferin (221.54 µg/ml) collected at 0.5 h after mangiferin administration (50 mg/kg, i.v.).

    Article Snippet: Assay conditions HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Injection

    HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).

    Journal: Molecular Vision

    Article Title: Pharmacokinetic study of mangiferin in rat plasma and retina using high-performance liquid chromatography

    doi:

    Figure Lengend Snippet: HPLC chromatograms of mangiferin in the eye. HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system. A COSMOSIL 5C 18 —MS—IIanalytical column (4.6 mm×250 mm, 5 μm) was used and operated at 25 °C. The mobile phase consisted of methanol −2% glacial acetic acid (40:60,v:v). Typical chromatograms of blank eye, blank eye spiked with mangiferin and I.S., and rat eye sample after injection of mangiferin are presented. Mangiferin and the I.S. were eluted at 5.6 and 12.16 min, respectively. The total run time was less than 30 min. A good separation of the I.S. and mangiferin was obtained under the specified chromatographic conditions. There is no disturbance from the background signals in the eye after the protein precipitation step. A : Typical chromatogram of blank eye sample. B : Typical chromatogram of blank eye sample spiked with standard mangiferin (1 μg/ml) and I.S. C : Typical chromatogram of eye sample containing mangiferin (5.63 µg/ml) collected at 1 h after mangiferin administration (50 mg/kg, i.v.).

    Article Snippet: Assay conditions HPLC separation was performed using the Agilent 1200 Series Rapid Resolution system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Injection